MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 12
·1-7
·
IF 5.479
Leng Yumin,Fu Yu,Lu Zhiwen,Sang Zhipei,Liu Kecheng,Du Chenxi,Ma Linfeng
A general approach is presented for synthesis of multicolored gold nanoparticles (GNPs) by Au(I)-mediated generation of interlocking rings in proteins and antibiotics. The Au(I) ions are shuttled from proteins to antibiotics, and this causes the formation of interlocking rings. The multicolored GNPs of different sizes were synthesized in the rings by using the rapid nucleation method. To take the unique colors of GNPs, a functional array was designed for the colorimetric determination and discrimination of antibiotics, specifically of amoxicillin, chlortetracycline, erythromycin, spiramycin, neomycin, thiamphenicol, gentamycin and lincomycin. The method is based on the “three color” (RGB) principle. The color response patterns are characteristic for each antibiotic and can be quantitatively differentiated by statistical techniques. The limits of detection (LOD, at S/ N = 3) for spiramycin (Sp) have been calculated to be 0.18 μM and 0.10 μM in water and milk, respectively. The good linear range (from 0.3 to 3.5 μM) has been used for the quantitative assay of Sp in a certified reference material.
Schematic presentation of gold nanoparticles (GNPs) synthesis via formation of interlocking rings in protein and antibiotics. The Au(I) ions mediate protein and antibiotics to be interlocking rings, which are quickly fixed via microwave reaction. The GNPs are synthesized and assembled in the rings.
研究领域:
生物医学工程
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纳米技术
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药物分析
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生物传感
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食品安全
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DOI
高影响因子
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
·
2023
·Vol. 110
·期 2
·1-8
·
IF 2.7
Wu Shaoxiong,Wang Lianyuan,Zhu Haiyan,Yuan Ling,Li Jian,Ge Liang,Zhang Hongpeng,Miao Ting,Cheng Zhenxing
The analysis of nerve agents is the focus of chemical warfare agent determination because of their extreme toxicity. A classical chemical colorimetric method, namely, the Schoenemann reaction, has been developed to detect G agents; however, it has not been utilized for VX analysis mainly because of its low peroxyhydrolysis rate. In this study, based on the mechanism of the Schoenemann reaction, a novel rapid quantitative determination method for VX was developed by optimizing the reaction conditions, such as concentrations of peroxide and the indicator, temperature, and reaction time. Using 2 ml 0.5 wt% sodium perborate as the peroxide source, 1 ml 0.1 wt% benzidine hydrochloride as the indicator, and 1 ml acetone as the co-solvent, VX and GD in ethanol or water solutions could be quantitatively analyzed within 15 min at 60°C. Further experiments based on 31 P NMR spectroscopy confirmed the existence of a peroxyphosphate intermediate during the GD assay. This quantitative colorimetry system for VX and GD analysis can be developed as a portable device for the water samples in fieldwork applications.
研究领域:
化学
·
环境科学
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Xiaoli Zhang,Zhaoguang Wang,Jing Yang,Yingying Li,Cheng Lu,Yanqiang Hao,Guanbo He,Yongjian Zhang,Qifang Song,Jun Long,Jiajie Liang,Yong Tang
Currently, producing NPSH after HPV infection of cells has been confirmed. These NPSH-containing substances accumulate around the urethral opening and are subsequently washed out with urine. Therefore, indirect detection of HPV infection by assessing NPSH levels in urine is feasible, but it has not been reported in detail so far. Here, an assay using phosphotungstic acid to oxidise and produce colour changes by NPSH in urine was developed. This assay enabled the rapid, non-invasive identification of HPV infection by detecting the metabolic byproduct NPSH produced by HPV-infected cells. Employing a smartphone-based device, developed using an ambient light sensor, reduces the cost and simplifies the operation associated with the colourimetric assay. The colourimetric assay was used to detect L-cysteine and L-ascorbic acid standard substance (as NPSH mimics), the limited of detection were 0.12 mM and 31.25 μM, respectively, with high reproducibility and stability. When this colourimetric assay was used to evaluate urine samples from individuals suspected of HPV infection, along with other at-home self-screening methods for HPV nucleic acid detection in urine, showed comparable sensitivity and specificity. Compared with nucleic acid detection in urine, this colourimetric assay is cost-effective, user-friendly, amenable to self-sampling, and enables testing at one’s convenience and location of choice, which is more suitable for home self-testing or population self-screening.
研究领域:
医学检验
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生物化学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Jianjun Du,Haoying Ge,Saran Long,Wen Sun,Jiangli Fan,Xiaojun Peng
Adenosine, as an endogenous molecule in organisms, plays an essential role in biological processes. Here, a plasmonic probe, creatinine-Ag + /gold nanoparticle (AuNPs), is assembled for adenosine detection based on synergistic coordination on AuNPs. The A 650 nm/520 nm values of AuNPs system change linearly with adenosine concentration over a range of 1.0–5.0 μM and the detection limits reached 45 nM. The adenosine detection is realized within 4 min. Furthermore, the quantitative detection of adenosine is realized by eyedropper (a function in Microsoft’s PowerPoint) for analyzing RGB value changes of colorimetric assay . Therefore, this sensor can provide accurate and rapid assay of adenosine in patients’ serum sample without complicated instrumentations.
研究领域:
生物化学
·
纳米医学
·
分析化学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Chunjuan Wang,Qin Guo,Ao Gao,Jingmei Zhang,Hailong Wan,Qianchen Liu,Junli Song
A smartphone-assisted fluorescent and colorimetric detection method for nitrite based on 2,3-diaminonaphthalene (DAN) and gold nanoclusters (AuNCs) paper strip was developed. DAN reacts with NO 2 - via a coupling reaction under acidic conditions to generate naphthotriazole (NAT), emitting strong blue fluorescence at 435 nm. The red fluorescence of AuNCs at 650 nm is quenched by the reduction of nitrite. The ratio of fluorescent intensity (I 435 /I 650 ) exhibits a good linear relationship with NO 2 - concentration (0.01–9.0 μg/mL), with LOD and LOQ of 2.3 μg/L and 8.7 μg/L, respectively. The fluorescent detection mode was successfully applied to the determination of nitrite in water and food samples, with recoveries ranging from 80.0 % to 103.0 %. Furthermore, a smartphone-integrated paper strip platform was designed for on-site colorimetric analysis. It was noteworthy that the strip's fluorescence color transitions dynamically from red to blue under ultraviolet light, thereby enabling visual semi-quantification.
研究领域:
分析化学
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生物传感
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环境监测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2023
·Vol. 28
·期 12
·4630
·
IF 4.6
Qing Li,Yumeng Guo,Xiangyi He,Guangli Li
The sensitive and accurate determination of glyphosate (Glyp) is urgently demanded because it is closely correlated with human health and environmental safety. In this work, we proposed a sensitive and convenient colorimetric assay by employing copper ion peroxidases for the detection of Glyp in the environment. Free Cu(II) ions displayed high peroxidase activity and can catalytically oxidize the colorless 3,3′,5,5′-tetramethylbenzidine (TMB) into blue oxTMB, resulting in an obviously visible discoloration reaction. Once the Glyp is added, the ability of copper ions to mimic peroxidase can be largely suppressed because of the generation of Glyp–Cu2+chelate. The favorable selectivity and sensitivity were demonstrated in the colorimetric analysis of Glyp. Furthermore, this rapid and sensitive method was successfully applied in the accurate and reliable determination of glyphosate in the real sample, holding promising applications in pesticide determination in the environment.Keywords:label-free;colorimetric analysis;copper ions;peroxidase-mimicking activity;glyphosate
研究领域:
环境科学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2020
·
IF 10.652
Ningyi Chen,Bingcai Pan
Toxic metal-organic complexes are ubiquitous in contaminated waters but more challenging than free ion counterparts for water decontamination. Currently, almost all the available analytical methods, including Au nanoparticles-mediated colorimetric assay, cannot discriminate metal-organic complexes from the total metal content, which greatly impedes the development of specific decontamination strategies for metal-organic complexes. In this study, we found that nitrilotriacetic acid (NTA) functionalized AuNPs could be used to determine various Cr(III) complexes (Cr(III)-lactate, Cr(III)-malonate, Cr(III)-oxalate, Cr(III)-tartrate, Cr(III)-citrate and Cr(III)-EDTA) in water. Mechanism study indicated that at acidic pHs (pH = 1.6), the protonation of organic ligands resulted in partial dissociation of Cr(III) from the corresponding complexes to expose its accessible orbitals for re-coordination with NTA. Such re-coordination induces the aggregation of the dispersed NTA-AuNPs and thereafter causes considerable changes both in color and UV–Vis spectrum of the solution. The nanosensor is highly selective to Cr(III)-organic complexes with detection limits of 0.029 μM for Cr(III)-lactate, 0.043 μM for Cr(III)-malonate, 0.35 μM for Cr(III)-oxalate, 0.079 μM for Cr(III)-tartrate, 2.58 μM for Cr(III)-citrate and 0.90 μM for Cr(III)-EDTA, respectively. The feasibility of the proposed colorimetric assay has been verified by using the authentic natural and industrial water samples. We believe this study will be of fundamental significance to push forward the application of Au NPs colorimetric assay in authentic waters contaminated by toxic metals.
研究领域:
环境科学
·
分析化学
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DOI
高影响因子
Journal of Materials Chemistry B
·
2019
·Vol. 7
·期 42
·6508-6514
·
IF 5.047
Hui Huang,Juan Bai,Jiao Li,Lulu Lei,Wenjing Zhang,Shujun Yan,Yongxin Li
In this work, a fluorometric and colorimetric analysis of alkaline phosphatase (ALP) activity was developed based on nanozymes. The nanozymes were composed of nucleotides (ATP, ADP and AMP) coordinated with copper ions. All three kinds of nanozymes (ATP-Cu, ADP-Cu and AMP-Cu) exhibited polyphenol oxidase (PPO)-mimic activity by catalyzing a chromogenic reaction of 2,4-dichlorophenol (2,4-DP) and 4-aminoantipyrine (4-AP). However, there were obvious differences in the PPO-like activity and the fluorescence of the three nanozymes produced from the same concentration of nucleotides (keeping the concentration of Cu2+ unchanged at 5 mM). The catalytic activities of produced ADP-Cu and AMP-Cu were obviously higher than that of ATP-Cu at a certain nucleotide concentration of 3 mM. In addition, when ATP was hydrolyzed into ADP and AMP by ALP, more nanozymes were produced and the catalytic activity of the system was enhanced, which resulted in an obvious increase of the colorimetric signal. The signal intensity was proportional to ALP concentration in the range of 0–30 U L−1, and the detection limit for ALP was 0.3 U L−1 from the colorimetric detection. Moreover, the fluorescence intensity of the produced nanozymes was also proportional to the ALP concentration in the range of 1–30 U L−1 and the detection limit was 0.45 U L−1 from the fluorescence detection. A fluorometric and colorimetric sensing ALP method was thus established. The method showed a high selectivity for ALP activity compared with proteins, amino acids and other interference components. Furthermore, the proposed method was also used to detect ALP activity in human serum samples, which showed great potential for diagnostic and practical purposes.
研究领域:
生物化学
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医学诊断
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分析化学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Chang Liu,Yanan Wu,Mengying Li,Fang Liu,Pengfei Kong,Haifeng Yang,Xinling Liu
Salivary d-alanine (d-Ala) and d-proline (d-Pro) are of concern for their potential in the noninvasive diagnosis of gastric cancer (GC). Most reports have succeeded in determining the total concentration of d-Ala and d-Pro. However, for personalized diagnosis and better elucidation of the underlying specific correlation of d-Ala (or d-Pro) with GC, it is desirable to determine the specific concentration of d-Ala or d-Pro. Herein, we propose an enantiomer-specific tandem assay of d-Ala based on the colorimetric reaction between 2,4-dinitrophenylhydrazine and pyruvic acid generated from the deamination of d-Ala catalyzed by d-amino acid oxidase, which is easily distinguished from l-form amino acids, d-Pro, and many other species. A linear concentration range is established from 20 to 400 μmol/L with a limit of detection of 1.01 μmol/L. Real saliva sample tests reveal that the levels of d-Ala in GC cases are remarkably higher than those in healthy individuals, which offers a simple and low-cost strategy for GC diagnosis. Simultaneously, the total concentrations of d-Ala and d-Pro in saliva are determined. Hence, the concentration of d-Pro and the proportion of d-Ala could be calculated, which further provides more molecule- and individual-specific information. This research may offer a convenient method for noninvasive diagnosis of GC and pave a new route to explore the potentials of rare d-form amino acids in disease diagnosis and treatment.
研究领域:
生物化学
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医学诊断
·
癌症研究
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 5
·1-13
·
IF 5.3
Hu Anqi,Chen Guoqing,Li Lei,Ma Chaoqun,Yang Taiqun,Gao Hui,Gu Jiao,Zhu Chun,Wu Yamin
Nitrogen, boron co-doped carbon quantum dots (gCQDs), and a coloration probe (PPD-NPs) with response to cobalt ions (Co 2+ ) were prepared by using 4-hydroxyphenylboric acid as the common precursor, with ethylenediamine and p-phenylenediamine (PPD) adopted as nitrogen-doped reagents, respectively. A noticeable brown-to-purple color change can be observed with the addition of Co 2+ , and a broad absorption band emerges at 535 nm. At the same time, gCQDs, which is introduced as the fluorescence signal source, will be significantly quenched due to the enhanced inner filtration effect, induced by the overlap between the emission spectrum of gCQDs and the emerging absorption band. Therefore, a colorimetric/fluorescent dual-mode sensing probe for Co 2+ is constructed by combining the recognition unit PPD-NPs and the fluorescent gCQDs into PPD-NP/gCQD. Under the optimized experimental conditions, the calculated limits of detection are 1.51 × 10 −7 M and 3.75 × 10 −7 M for the colorimetric mode and the fluorescence mode, respectively, well qualified for the determination of Co 2+ maximum permitted level in drinking water. The feasibility of the proposed method has been verified in tap water, lake water, and black tea samples. Graphical Abstract
研究领域:
分析化学
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材料科学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Gang Chen,Can Fang,Hui Hui Chai,Ying Zhou,Wan Yun Li,Ling Yu
Smartphones-based colorimetric analysis systems are favoured for point-of-care testing applications. However, the image quality typically suffers from nonuniform and non-reproducible lighting, which negatively affects the accuracy of analyses. The present work proposed a power-free imaging box to utilize the light source of smartphone. The imaging box effectively eliminated interference arising from ambient light, while a V-shaped optical pipe coupled to the light source of smartphone was adopted to redirect the light to the sides of the box to eliminate the direct reflection of light from the surface of the target. This configuration was verified by numerical simulations. The specular reflection of the microfluidic chip was eliminated by establishing ideal diffuse light conditions. Moreover, the color images of a glucose testing on a paper strip and an enzyme-linked immunosorbent assay (ELISA) on transparent microchannels were captured with the proposed configuration, resulting a reduced assay variation and improving the lowest detection limit. The proposed imaging box improves the detection limit of colorimetric analysis, which highlights the potential of smartphone-based colorimetric analysis systems in point-of-care testing applications.
研究领域:
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Nano Research
·
2015
·Vol. 8
·期 12
·3853-3863
·
IF 7.01
Zhang Juan,Lv Jun,Wang Xiaonan,Li Defeng,Wang Zhaoxia,Li Genxi
Here we developed a saccharic colorimetric method based on the combination of chemoselective ligation and enzyme-specific catalysis using aminooxy/hydrazine-functionalized gold nanoparticles (AO/AuNPs or H/AuNPs). In the detection of galactose (Gal), galactohexodialdose (GHDA), the galactose oxidase (GalOx)-catalyzed product, has an aldehyde group, which allows it to chemoselectively react with an aminooxy or hydrazine group at the outer layer of AO/AuNPs or H/AuNPs by oxime/hydrazone click chemistry to form oxime or hydrozone. Consequently, through the specific recognition of 1,4-phenylenediboronic acid (PDBA) on cis-diols, GHDA, which contains two pairs of hydroxyls in the cis form, can bind not only with AO/AuNPs or H/AuNPs, but also with PDBA to form boronate diester, thereby triggering the aggregation of AuNPs and causing the corresponding color change. As GalOx catalyzed specific substrates, the amount of Gal correlated with the production of GHDA and the extent of AuNPs aggregation, thus allowing a simple and easily operatable colorimetric method for Gal detection to be developed. Under the optimized experimental conditions, the ratios of absorbance at a wavelength of 617 nm to that at 536 nm vary linearly with the logarithmic values of Gal concentrations within a wide range of 500 nM to 5 mM. Moreover, this colorimetric method shows anti-interference capability and high sensitivity with a detection limit of 21 nM. Thus, a universal platform for accurate and specific colorimetric analysis can be established through the integration of chemoselective ligation with enzyme specific catalysis.
研究领域:
生物化学
·
纳米生物学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）