Hyperuricemia (HUA) has gradually become a public health burden as an independent risk factor for a variety of chronic diseases . Herein, a user-friendly point-of-care (POC) detection system (namely “Smart-HUA-Monitor”) based on smartphone-assisted paper-based microfluidic is proposed for colorimetric quantification of HUA urinary markers, including uric acid (UA), creatinine (CR) and pH. The detection limits of UA and CR were 0.0178 and 0.5983 mM, respectively, and the sensitivity of pH were 0.1. The method was successfully validated in artificial urine samples and 100 clinical samples. Bland-Altman plots showed a high consistency between μPAD and the testing instruments (HITACHI 7600 Automatic Analyzer, URIT-500B Urine Analyzer and AU5800B automatic biochemical analyzer) in hospital. Smart-HUA-Monitor provides an accurate quantitative, rapid, low-cost and reliable tool for the monitoring and early diagnosis of HUA urine indicators.
Quanming Xu,Feng Zhang,Guizhen Luo,Jiayi Liu,Xue He,Zemiao Wang,Mingdian Yu,Zongwen Wang
The growth of gold nanobipyramids (AuNBPs) provides a superior colorimetric quantification method for point-of-care testing due to their superior growth uniformity, high-resolution multicolor response, and excellent seed storage stability. However, conventional growth methods face significant limitations in sensing applications due to their reliance on a single reductant and stringent acidic conditions. This study systematically investigated the reductant mechanism in AuNBP growth, revealing that the pH-regulated redox potential is crucial for achieving anisotropic AuNBP growth. By employing nine different reductants, the growth pH was expanded from 1.5 to 8.5. Notably, dihydroxybenzene-mediated systems exhibited a slender morphology, reduced reductant consumption, enhanced stability, and comparable analytical performance compared to the conventional growth system. Among these systems, the hydroquinone (HQ)-mediated system was integrated with horseradish peroxidase (HRP)-catalyzed reactions in a pH-compatible manner. By incorporating this integrated system into a commercial HRP immunoassay, a novel multicolor immunosensor was developed for thyroglobulin (Tg) detection. This immunosensor exhibited a distinct nine-color gradient across a ten-fold Tg concentration range (0.1-1.3 ng/mL), achieving a visual detection limit of 0.1 ng/mL and an instrumental detection limit of 29 pg/mL. It accurately detected Tg in human serum with good recoveries of 94.1%-104.8% and signal stability for six hours. The results correlated well with the commercial immunoassay while offering superior visual readout for point-of-care settings. These findings validate the applicability of the HQ-mediated system in converting conventional single-color HRP immunoassays to multicolor immunosensors. Furthermore, expanding reductant selection and pH adaptability in AuNBP growth offers more possibilities for developing versatile pH-flexible multicolor sensors.
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