FOOD CHEMISTRY
·
2025
·
IF 9.8
Ruiqi Zou,Junxiao Shi,Qi Lu,Chunyan Sun,Haiqing Ye,Xu Yan,Fangjie Tian,Hongxia Li
Given the potential dangers of organophosphorus pesticides to food safety and human health, the development of a reliable and precise detection platform for pesticides is essential. In this study, we present a novel ‘armor-plating’ laccase-mimetic catalyst (DNA-Cu@MOFs)-based colorimetric platform, which enables stable and selective pesticide detection. The DNA-Cu@MOFs enhance catalytic stability and overcome pH limitations, enabling effective catalysis under neutral and alkaline physiological conditions, making them well-suited for practical applications in biosensor development. By combining the catalytic properties of DNA-Cu@MOFs with a high-affinity biorecognition element (acetylcholinesterase), the platform achieves a linear detection range of 3.0–90 ng mL −1 for chlorpyrifos, with a detection limit of 0.75 ng mL −1 . Notably, this platform demonstrates significant stability in chlorpyrifos detection even in the presence of environmental interferents. This robust colorimetric platform offers new possibilities for pesticide detection and provides a solid foundation for the development of comprehensive and accurate pesticide monitoring systems.
研究领域:
生物传感
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环境科学
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食品科学
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2018
·
IF 2.88
Fei Qu,Zeqiu Chen,Jinmao You,Cuihua Song
Human telomere DNA plays a vital role in genome integrity control and carcinogenesis as an indication for extensive cell proliferation. Herein, silver nanoclusters (Ag NCs) templated by polymer and unmodified gold nanoparticles (Au NPs) are designed as a new colorimetric platform for sensitively differentiating telomere DNA with different lengths, monitoring G-quadruplex and dsDNA. Ag NCs can produce the aggregation of Au NPs, so the color of Au NPs changes to blue and the absorption peak moves to 700 nm. While the telomere DNA can protect Au NPs from aggregation, the color turns to red again and the absorption band blue shift. Benefiting from the obvious color change, we can differentiate the length of telomere DNA by naked eyes. As the length of telomere DNA is longer, the variation of color becomes more noticeable. The detection limits of telomere DNA containing 10, 22, 40, 64 bases are estimated to be 1.41, 1.21, 0.23 and 0.22 nM, respectively. On the other hand, when telomere DNA forms G-quadruplex in the presence of K + , or dsDNA with complementary sequence, both G-quadruplex and dsDNA can protect Au NPs better than the unfolded telomere DNA. Hence, a new colorimetric platform for monitoring structure conversion of DNA is established by Ag NCs-Au NPs system, and to prove this type of application, a selective K + sensor is developed.
研究领域:
生物化学
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纳米医学
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分子生物学
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Yun Zhang,Wenqing Tan,Yang Zhang,Huili Mao,Shuyou Shi,Liangwei Duan,Hui Wang,Junping Yu
To develop a specific method for the detection of S. aureus , chicken anti-protein A IgY was adopted for specifically capturing S. aureus , depending on the specific recognition of staphylococcal protein A (SPA) by chicken anti-protein A IgY, which can eliminate the interference from protein G-producing Streptococcus . HRP labeled IgG , Fc region of which has a high affinity towards SPA, was paired with IgY for the colorimeter analysis of the system. By optimizing the system, a super-low detection limit of 11 CFU of S. aureus in 100 μL PBS without enrichment, with a linear range from 5.0 × 10 2 CFU mL −1 to 5.0 × 10 4 CFU mL −1 was obtained. The entire assay was accomplished in less than 90 min and no cross-reactivity with the other tested bacterial species was observed. Moreover, the developed assay has been applied for the detection of S. aureus in three different types of real samples (sodium chloride injection, apple juice and human urine) with satisfactory results. To the best of our knowledge, it is the first time to report using chicken anti-protein A IgY and any IgG to detect S. aureus based on the dual-recognition mode of SPA. The novel method opened up a way for monitoring S. aureus in food samples with high sensitivity, specificity and simple operation.
研究领域:
微生物学
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分析化学
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食品安全
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Mengyang Zhou,Haoyu Wang,Chao Li,Chao Yan,Panzhu Qin,Lin Huang
Single base mutations detection is crucial for the diagnosis and treatment of cancer. However, the current methods with poor selectivity and sensitivity required large instruments, which are difficult to meet clinical demands. Herein, we develop a CRISPR/Cas9 based visual colorimetric platform to specifically detect all single base mutations. In this strategy, the Recombinase Polymerase Amplification (RPA) was firstly used to amplify the target, and introduced the PAM site in the target DNA sequence by designing the point mutation primer, thus achieving detection for all single base mutations by the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9-mediated specific recognition. With the help of CRISPR/Cas9 system, those RPA products can release single strand DNA to hybridize with the padlock probe and trigger rolling circle amplification (RCA). Based on the magnetic separation, HRP-gold nanoparticles complex (hGNPs) and biotin modified probe (Bio-probe) were further used to achieve enhanced visual variations assay by hybridizing with RCA products. Benefiting from the RPA assisted triple signal amplification, this method not only showed enhanced sensitivity with a limit of detection (LOD) as low as 0.2 fM and 0.01% of KRAS-G12D mutation percentage, but the specificity against KRAS-G12D mutation also be synergistically enhanced by combining the CRISPR/Cas9-mediated specific recognition with the specific T4 ligation reaction of RCA system. Furthermore, this system has been successfully used to visually detect genome in serum, suggesting its great potential for point-of-care diagnosis in clinical.
研究领域:
分子生物学
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生物技术
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医学诊断
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癌症研究
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2024
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IF 4.1
Jingjing Chen,Zhixiang Li,Xiaoqi Li,Yingxiang Du
A fluorescence sensor was developed based on NH 2 -MIL-101(Fe) and silicon-doped carbon quantum dots for the analysis of copper ions. Copper ions enhanced the fluorescence intensity of doped silicon-carbon quantum dots and decreased the intensity of NH 2 -MIL-101(Fe). The addition of copper ions resulted in fluorescence resonance energy transfer, which caused a reverse change in the fluorescence signal. The sensor based on the above mechanism can eliminate false positive interference. The fluorescence sensing system had a remarkable linear range of 0.25–5 µM for copper ions and a detection limit of 11 nM. It was then applied to water samples, resulting in recoveries ranging from 95.43 % to 103.1 %. The successful construction of this optical sensor not only broadened the way to detect copper ions but also provided a new idea for the detection of other targets mediated by copper ions. Finally, an attempt was made to construct a smartphone-assisted colourimetric platforms to detect copper ions in daylight.
研究领域:
分析化学
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环境科学
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材料科学
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纳米技术
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Chunda Liu,Longai Liang,Yang Yu,Yang Hou,Maosen Yuan,Yuting Zhuang,Jinyi Wang,Yue Zhang
β-lactoglobulin (β-lg) is the major allergen in dairy products, and poses a significant threat to special people including infants and young children. Therefore, a convenient, cost-efficient, and aptamer-free colorimetric sensing platform is developed for β-lg assay based on a germanium/zinc bimetallic oxide nanozyme (GeO 2 /ZnO). The CTAB-assisted intercalation growth of ZnO nanoparticles between GeO 2 layers endows GeO 2 /ZnO with enhanced peroxidase-mimicking activity and β-lg affinity. By virtue of multiple recognition-meditated specific protein corona formation, the catalytic activity of GeO 2 /ZnO is greatly deteriorated in the presence of β-lg. The selective discrimination and accurate detection of β-lg in dairy samples is achieved with a detection limit of 14.1 nM, and a portable kit is further established for point-of-care testing and visual colorimetric analysis. This study not only provides a promising strategy for allergen assay, but paves an avenue for engineering the abiotic protein affinity sensors for food safety analysis.
研究领域:
食品安全
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分析化学
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纳米技术
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生物传感器
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2015
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IF 4.513
Chaohui Chen,Ningxing Li,Jingwen Lan,Xinghu Ji,Zhike He
A target-catalyzed hairpin assembly (CHA) and graphene/Au-NPs hybrids-based platform has been developed for the determination of DNA . This new sensor not only avoided any labeling but also reduced the background signal. In the absence of target, the assembly of H1 and H2 couldn't be triggered. The catalytic activity of graphene/Au-NPs hybrids was inhibited by adsorption of H1 and H2, leading to the “inactive” hybrids unable to catalyze the oxidation reaction of 3,3′,5,5′-tetramethylbenzidine (TMB). However, with the addition of target DNA, the target-catalyzed hairpin assembly was initiated and produced plenty of H1–H2 duplex, which had a weak binding affinity with the graphene/Au-NPs. Thus, the protected interface of graphene/Au-NPs hybrids became active and catalyzed the oxidation reaction of TMB accompanied with a colorless to-blue color change. This approach exhibited good sensitivity and specificity for target DNA with a detection limit of 5.74 × 10 −11 M, and realized the assay of target DNA in human serum samples. Besides, this sensor could be further expanded to detect viruses or proteins by adapting the corresponding aptamers, showing great potential in biochemical detections.
研究领域:
生物化学
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纳米技术
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分子生物学
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分析化学
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2023
·
IF 6.1
Jun Xu,Ying Wang,Menghua Huang,Xiaojuan Xu,Yueyun Zeng,Xuan Luo,Shicheng Pei,Keming Xu,Wenying Zhong
Compared to conventionally synthesized organic compounds, peptides with amphiphiles have unique advantages, especially in self-assembly. Herein, we reported a peptide-based molecule rationally designed for the visual detection of copper ions (Cu 2+ ) in multiple modes. The peptide exhibited excellent stability, high luminescence efficiency, and environmentally responsive molecular self-assembly in water. In the presence of Cu 2+ , the peptide undergoes an ionic coordination interaction and a coordination-driven self-assembly process that leads to the quenching of fluorescence and the formation of aggregates. Therefore, the concentration of Cu 2+ can be determined by the residual fluorescence intensity and the color difference between peptide and competing chromogenic agents before and after Cu 2+ incorporation. More importantly, this variation in fluorescence and color can be presented visually, thus allowing qualitative and quantitative analysis of Cu 2+ based on the naked eye and smartphones. Overall, our study not only extends the application of self-assembling peptides but also provides a universal method for dual-mode visual detection of Cu 2+ , which would significantly promote point-of-care testing (POCT) of metal ions in pharmaceuticals, food, and drinking water .
研究领域:
分析化学
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材料科学
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环境科学
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生物技术
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Haiyan Cao,Yuwan Lu,Xiaodan Zhang,Wenfei Dong,Wenbing Shi,Yuming Huang
The extensive applications of peroxymonosulfate (PMS) in the field of environmental pollutant treatment based on advanced oxidation processes (AOPs) have prompted a high demand for advances in the probe ranging from improving their performances to strengthening their practicalities. Here, a multisignal-output probe of PMS was achieved by the unconventional application of traditional chromogenic system, 3,3′,5,5′-tetramethylbenzidine with Co 2+ ions (Co 2+ -TMB). It works on the trace Co 2+ -catalyzed specific conversion of a colorless TMB into a blue oxidation product (oxTMB) with PMS as the oxidizing agent , accompanied by the enhanced absorption value of oxTMB at 652 nm and decreased fluorescence intensity of TMB at 405 nm. The change of color was directly observed. The combination of a hydrogel with a colorimetric platform favors the real-time and convenient sensing of PMS in the concentration range of 0.98 to 130.29 μM. A good linear relationship between the PMS concentration and both the absorption and fluorescence intensity was obtained with an r 2 value of 0.999, and a detection limit of 0.03 μM for fluorescence and 0.11 μM for spectrometry . Such a multisignal output probe with the advantages of simplicity, convenience, high sensitivity and stability improves the reliability and practical adaptability. This study successfully applied a valuable strategy for sensing PMS, and verified its availability in detecting the residual quantity of PMS in pollutant water treated by AOPs and PMS concentrations in actual water samples with peroxydisulfate (PDS).
研究领域:
环境科学
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化学工程
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分析化学
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水处理
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产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2020
·Vol. 8
·期 30
·6459-6468
·
IF 5.344
Xiaodan Zhang,Yuwan Lu,Qiumeng Chen,Yuming Huang
A self-templated strategy was adopted to design hollow Co3O4/MO3 (M = Mo, W) mixed-metal oxides via the Mo or W doping of ZIF-67, and subsequent pyrolysis under an atmosphere of air at a low temperature of 450 °C. The hollow Co3O4/MO3 (M = Mo, W) mixed-metal oxides displayed tunable oxidase-like and peroxidase-like activities able to efficiently catalyze the oxidation of TMB to generate a deep blue color in the absence or presence of H2O2. Relative to that of the un-doped Co3O4, the oxidase mimic activity of the Mo-doped Co3O4 increased to 1.3 to 2.1-fold, while its peroxidase mimic activity increased to 7.1 to 19.9-fold, depending on different Mo doping amounts. The oxidase mimic activity of the W-doped Co3O4 increased to 2.1 to 2.3-fold, while its peroxidase mimic activity increased to 4.8 to 5.9-fold, depending on the different W doping amounts. The Mo- and W-doped Co3O4 nanohybrid exhibited both higher O2 and H2O2 activating capability, and their H2O2 activating capacity was superior to the O2 activating capability. Furthermore, the Mo- and W-doped Co3O4 nanohybrids exhibited similar O2 activating abilities, while the Mo-doped one displayed a higher H2O2 activating capability than the W-doped one. The discrepant peroxidase-like nature of Mo- and W-doped Co3O4 nanohybrids is likely attributed to their different catalytic mechanisms. The peroxidase-like activity of Mo-doped Co3O4 is highly related to the ˙OH free radical, while that of W-doped Co3O4 is likely ascribed to the electron transfer between TMB and H2O2. The Km values of Co3O4/MoO3 for TMB and H2O2 were 0.0352 mM and 0.134 mM, which were 3.2- and 1.9-fold lower than that of pure Co3O4, respectively. A Co3O4/MoO3-based colorimetric platform was developed for the determination of H2O2 in the 0.1–200 μM range, with a limit of detection of 0.08 μM (3σ). Based on the thiocholine (TCh) inhibition of the excellent peroxidase-like activity of Co3O4/MoO3 and the TCh generation via acetylcholinesterase (AChE) catalyzed hydrolysis of acetylthiocholine chloride (ATCh), the colorimetric platform was extended to screen AChE activity and its inhibitor.
研究领域:
纳米材料
·
酶模拟
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分析化学
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生物传感器
·
催化
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）