ACS Omega
·
2025
·
IF 4.3
Hongjing Yang,Zhipeng Zang,Zhen Wang,Kai Song,Yunzhen Gao,Jinbin Wei,Lu Han,Jiandong Zhang,Chen Liu
Starch and cellulose are pivotal determinants of tobacco quality. In this work, starch and cellulose in flue-cured tobacco leaf were extracted through a sequential extraction process. First, an aqueous solution of tobacco leaves was prepared, and starch was extracted by boiling the leaves in water. The precipitate was separated, and the cellulose within it was hydrolyzed using a sulfuric acid solution. Then, starch content was quantified by a colorimetric reaction with iodine solution, while cellulose content was determined by using anthrone as a chromogenic agent. The reaction conditions were optimized to ensure accuracy, and the recovery rate and relative standard deviation (RSD) were calculated under these optimum conditions. The recovery rates for starch and cellulose in the tobacco samples ranged from 90 to 105%, with the RSD of interday and intraday determination being less than 5%. These results demonstrate that the proposed method is both sensitive and reproducible, making it suitable for the rapid and precise quantification of starch and cellulose in tobacco samples.
研究领域:
植物学
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分析化学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2014
·Vol. 6
·期 19
·7973-7977
·
IF 1.938
Yingfeng Qin,Liangliang Zhang,Gaojie Ye,Shulin Zhao
A homogeneous label-free colorimetric sensing strategy for bleomycin (BLM) assay was developed on the basis of BLM enhanced Fe(II)–H2O2–ABTS reaction. Fe(II) exhibits a catalytic effect on H2O2-mediated oxidation of ABTS by Fenton chemistry. BLM·Fe(II) complex possessed a significantly higher catalytic ability than Fe(II) probably because of the further formation of more active “activated oxo-iron-bleomycin complex”. The present method was convenient without any complicated chemical synthesis, modification, or tedious experimental procedures. Analysis results can be seen with the naked eye and monitored by UV-vis spectra. The peak absorbance exhibits a linear relationship with BLM concentrations from 25 nM to 1 μM, and a detection limit of 16 nM can be achieved. The designed colorimetric strategy may provide a promising alternative for BLM detection in clinical samples.
研究领域:
分析化学
·
生物医学
·
临床检验
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产品关联
DOI
高影响因子
MOLECULES
·
2021
·Vol. 26
·期 9
·2738
·
IF 4.412
Xiang Lai,Yu Han,Jie Zhang,Jinyu Zhang,Weifeng Lin,Zhiwei Liu,Longgang Wang
The sensitive and selective detection of dopamine (DA) is very important for the early diagnosis of DA-related diseases. In this study, we reported the colorimetric detection of DA usingGanoderma lucidumpolysaccharide (GLP) stabilized platinum nanoclusters (Ptn-GLP NCs). When Pt600-GLP NCs was added, 3,3’,5,5’-tetramethylbenzidine (TMB) was rapidly catalyzed and oxidized to blue oxTMB, indicating the peroxidase-like activity of Pt600-GLP NCs. The catalytic reaction on the substrate TMB followed the Michaelis-Menton kinetics with the ping-pong mechanism. The mechanism of the colorimetric reaction was mainly due to the formation of hydroxyl radical (•OH). Furthermore, the catalytic reaction of Pt600-GLP NCs was used in the colorimetric detection of DA. The linear range for DA was 1–100 μM and the detection limit was 0.66 μM. The sensitive detection of DA using Pt-GLP NCs with peroxidase-like activity offers a simple and practical method that may have great potential applications in the biotechnology field.Keywords:dopamine;detection;platinum;colorimetric;Ganoderma lucidumpolysaccharide
研究领域:
生物技术
·
医学诊断
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Chenjing Xu,Jinpo Xie,Lian Yu,Bin Shu,Xiaogang Liu,Siping Chen,Qinglan Li,Shaohai Qi,Suqing Zhao
Vibrio vulnificus infection caused by contaminated aquatic products and seawater can lead to severe disease and high mortality. The development of a rapid and sensitive detection method for Vibrio vulnificus is vital to effectively prevent infection in advance. In this study, CeO 2 @PtRu with high peroxidase activity was used to construct a colorimetric immunoassay for Vibrio vulnificus detection by conjugating polyclonal antibodies via the biotin-streptavidin system. The developed colorimetric biosensor for Vibrio vulnificus demonstrated rapid operability and good sensitivity with a detection range from 10 4 CFU/mL to 10 9 CFU/mL, and the limit of detection (LOD) is 193 CFU/mL. Moreover, the colorimetric biosensor showed excellent specificity and good recoveries from 98.70% to 102.10% with RSD < 7.45% for spiked real samples. This novel CeO 2 @PtRu-based colorimetric biosensor has great application potential for the sensitive detection of Vibrio vulnificus in seafood.
研究领域:
微生物学
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免疫学
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生物传感技术
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ANALYTICAL CHEMISTRY
·
2020
·Vol. 75
·期 3
·330-342
·
IF 0.84
Haishuang Jia,Liu Wenfang,Li Na,Wang Jun,Song Youtao
Abstract Two novel methods for spectrophotometric determination of Cu 2+ ion using synthesized and characterized furfuraldehyde fluorescein diacetate hydrazone ( FFDH ) and furfuraldehyde fluorescein hydrazone ( FFH ) have been proposed. Addition of Cu 2+ to FFDH and FFH solutions results in a rapid color change from colorless to dark yellow. Experimental results show that FFH and FFDH both can provide a rapid, selective and sensitive response to Cu 2+ within a linear dynamic range of 7–33 µM and the detection limits of 1.1 × 10 –7 and 7 × 10 –8 M, respectively. Apparently, the detection limit of FFDH is lower than that of FFH, which indicates that FFDH forms a more stable complex with Cu 2+ . In addition, the detection of Cu 2+ in water samples was also studied; as a result, the developed FFDH was found to be more applicable than FFH for spectrophotometric determination of Cu 2+ in environmental water samples. Particularly, as a lipid chemical compound, FFDH may be transported across the cell membrane to detect Cu 2+ in vivo. It is of great significance in many fields, such as biology, biochemistry, medicine and environment.
研究领域:
分析化学
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环境科学
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生物化学
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Xinke Wang,Xia Gao,Xiaodong Lin,Shuo Zheng,Yong Yan,Shuo Wang,Yaqing Liu
Silver ion pollution is a great threat to global environment and public healthcare today. Thus, quick, portable and sensitive assays for Ag + tracing are highly needed. Herein, a reliable fluorescent and colorimetric dual-channel assay has been constructed for Ag + detection by utilizing a terbium-based fluorescent nanomaterial (named as Tb-DPA) and a Pt/Pd nanoflower (Pt/Pd NF)-triggered reaction between N-ethyl-N-(3-sulfopropyl)-3-methylaniline sodium salt (TOPS) and 4-amino-antipyrine (4-AAP). Initially, in the sensing system containing 4-AAP, TOPS, Tb-DPA and Pt/Pd NF, TOPS and 4-AAP is catalyzed by Pt/Pd NF to produce a purple compound (called as PC1), which endows a broad UV absorption that can fully cover the emission band of Tb-DPA. Thus, the system exhibits a high UV absorption (originating from PC1) and a low fluorescence intensity (originating from Tb-DPA) which has been quenched by PC1 through a filtering effect. However, when the system meets Ag + , the oxidase-like activity of Pt/Pd NF will be inhibited, leading less amount of PC1. As a result, the system exhibits a decreased UV absorption and a recovered fluorescence intensity, both of which can be used for Ag + detection, reporting low detection limits of 3.63 nM and 1.63 nM, respectively. Specially, results from the dual-channel assay can mutually validate each other, improving the detection reliability. Moreover, this dual-mode assay shows good capability toward Ag + detection in real samples, illustrating the potential for practical applications.
研究领域:
环境科学
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分析化学
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DOI
高影响因子
RSC Advances
·
2017
·Vol. 7
·期 55
·34746-34754
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IF 3.108
Ziwei Hu,Mingshan Xie,Danting Yang,Dong Chen,Jingyi Jian,Haibin Li,Kaisong Yuan,Zhengjin Jiang,Haibo Zhou
Berberine is an important anti-inflammatory drug widely used for the treatment of heart and intestinal disorders. The quantification of berberine is usually required in clinic medical assays. Herein, we report simple, fast and sensitive colorimetric visualization of berberine in human plasma using citrate-stabilized gold nanoparticles (AuNPs). Upon exposure to μg mL−1 levels of berberine, the AuNP solution exhibits a highly sensitive color change from red to blue and rapid aggregation kinetics within the initial 10 min, which can be directly seen with the naked eye and monitored by UV-vis absorbance spectroscopy. As confirmed by comparison with potential coexisting foreign substances, the presence of positively charged berberine induces the aggregation of the AuNPs owing to the electrostatic attraction and coordination interactions between the ring nitrogen atoms of berberine and the AuNPs, causing a color change of the AuNP suspension. Further evidence is that the sensitivity is significantly improved when NaHSO4 is added to promote the ligand exchange between citrate and berberine at the surface of the AuNPs. The NaHSO4-optimized AuNP system provides a simple colorimetric assay for the rapid detection of berberine in human plasma. The limit of detection (LOD) is 0.24 μg mL−1 by the naked eye and 0.06 μg mL−1 by UV-vis spectroscopy.
研究领域:
药理学
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分析化学
·
纳米医学
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DOI
高影响因子
MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 7
·2249-2254
·
IF 4.58
Huang Conghui,Wang Shuzhan,Zhao Wenfeng,Zong Chenghua,Liang Aiye,Zhang Qingquan,Liu Xiaojun
The authors describe a colorimetric approach for on-site monitoring of histamine based on the use of gold nanoparticles (AuNPs) that have a negatively charged surface due to the presence of adsorbed citrate ions. Histamine has two basic functional groups, an aliphatic amino group and an imidazole ring. Under the experimental conditions, the protonated aliphatic amino group drives the imidazole ring into close proximity to the AuNPs due to electrostatic attraction. This accelerates the replacement of citrate ions by the imidazole ring because of the strong affinity between imidazole and AuNPs. As a consequence, the two groups synergistically induce the aggregation of the AuNPs and trigger a visible color change from red to blue. The minimum visually detectable histamine concentration is 1.81 μM, which is comparable to the limit of detection (LOD) of the electrochemical approaches at a signal-to-noise ratio of 3:1. When using absorbance at 522 nm, the LOD is lowered to 38 nM. The method was applied to the determination of histamine in fish samples.
Schematic of the detection scheme: Histamine induces gold nanoparticle aggregation and triggers a visible color change. The minimum visually detectable histamine concentration by bare eye is 1.81 μM. When using absorbance at 522 nm, the LOD is lowered to 38 nM.
研究领域:
分析化学
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食品科学
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DOI
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 7
·1-7
·
IF 5.705
Liu Qingju,Han Ping,Gong Wenwen,Wang Hui,Feng Xiaoyuan
A method is described for the determination of the pesticide chlorothalonil (CLT). It is based on the finding that citrate-capped gold nanoparticles (AuNPs) undergo aggregation on exposure to chlorothalonil. This is accompanied by a visually detectable color change from wine red to blue. The effect is due to the interaction of the cyano group of chlorothalonil with gold nanoparticles. The assay may also be performed by using a spectrometer. The ratio of absorbances at 700 nm and 520 nm (A 700 /A 520 ) linearly drops in the 5 to 100 ng·mL −1 CLT concentration range, with a 3.6 ng·mL −1 detection limit. This is below the Chinese guideline value for cucumber. The method is rather simple and does not require any modification of the AuNPs or the utilization of antibody. It was successfully applied to the determination of CLT in (spiked) cucumber samples. Recoveries ranged from 80.4 to 97.4%, and the analytical results compared well with those obtained by HPLC.
Schematic of the assay. The strong interaction of the cyano group of acetamiprid with gold nanoparticles (AuNPs) via Au-N bond induces the aggregation of gold nanoparticles, and this is accompanied by a color change from red to purple.
研究领域:
分析化学
·
环境科学
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2025
·
IF 6.1
Lijun Wang,Jiangchuan Tang,Shanshan Ren,Haixia Hu,Xiao Yang,Xianggui Chen
Although many colorimetric methods have been developed for on-site pesticide detection, most exhibit low sensitivity and limited resistance to interference. To address these issues, this study developed a novel dual-mode colorimetric-ratiometric fluorescence method for isocarbophos (ICP) detection using sulfur and nitrogen co-doped carbon nanodots (S, N-CDs) to inhibit catalytic Fe 3 O 4 @Cu-BTC nanozyme activity. The synthesized Fe 3 O 4 @Cu-BTC nanozymes with higher peroxidase-like activity were inhibited by S, N-CDs. The inhibitory mechanism was deeply investigated and was ascribed to (1) the restriction of the reaction between Fe 3 O 4 @Cu-BTC and hydrogen peroxide (H 2 O 2 ), which decreased the ·OH production, and (2) the ·OH scavenging ability of the S, N-CDs, forming the basis of the dual-mode method. The Fe 3 O 4 @Cu-BTC/Apt-complementary strand (CS)/S, N-CDs hybridization complexes demonstrated minimal to no peroxidase-like activity, preventing H 2 O 2 from oxidizing o -phenylenediamine (OPD) to produce yellow fluorescent 2,3-diaminophenazine (DAP). Meanwhile, the detection system demonstrated a low fluorescence intensity ratio (I 575 /I 465 ) at an excitation wavelength of 390 nm. However, the presence of ICP caused the CS/S, N-CDs to separate from the Fe 3 O 4 @Cu-BTC/Apt due to the high affinity between Apt and ICP. Then, the Fe 3 O 4 @Cu-BTC/Apt-ICP complexes recovered peroxidase-like activity, which promoted DAP production, accompanied by a pronounced fluorescence intensity ratio (I 575 /I 465 ). In optimum conditions, the detection limits (LODs) were 2.79 ng/mL (colorimetric) and 0.26 ng/mL (ratiometric fluorescence), respectively. The feasibility of the proposed method was verified via three spiked samples, yielding recovery rates of between 86.08 % and 112.64 %, indicating the significant potential for the highly sensitive, accurate on-site detection of ICP in actual samples.
研究领域:
分析化学
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环境科学
·
生物医学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Wenju Yan,Xuefei Qin,Xueqing Sang,Xueying Zhou,Yanying Zheng,Yali Yuan,Yun Zhang
A dual-mode sensing platform for Pb 2+ was constructed based on the dual optical channels of Au NPs system with the amplification of DNAzyme, and it was successfully applied for Pb 2+ determination in preserved egg with satisfactory results. The presence of Pb 2+ activated the DNAzyme and induced the dispersion change of Au NPs in high salt concentration. The sequent absorption change of Au NPs was translated to the fluorescence change of carbon dots through FRET, and the scattering change was transferred to grey value of images involving the Tyndall effect. Thus, a sensing platform based on fluorescence and colorimetric dual-technique was achieved for Pb 2+ detection, under the optimized conditions. With the assistance of DNAzyme, the linear range of fluorometric and colorimetric method were 2.0 × 10 -14 ∼ 8.0 × 10 -10 mol/L and 2.4 × 10 -13 ∼ 9.5 × 10 -9 mol/L, respectively. The dual-mode sensing platform demonstrated its promising application in the environmental monitoring and food safety field.
研究领域:
分析化学
·
环境科学
·
食品安全
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Tingting Liu,Yuhao Li,JingJing Gu,Lei Zhang,Feiyue Qian,BinRong Li,Xuedong Wang
Modulation of the nanozyme's catalytic activity is crucial for its real applications in detecting target analytes. Herein, we fabricated the nanocomposite (NSC/Co 6 Ni 3 S 8 ) of N, S co-doped carbon and Co 6 Ni 3 S 8 by a facile sol-gel approach. Compared to NSC/Ni 9 S 8 , NSC/Co 6 Ni 3 S 8 with bimetallic active sites displayed better enzyme-mimetic activity. This nanocomposite could catalyze O 2 to form ·O 2 − and oxidize colorless 3, 3′, 5, 5′-tetramethylbenzidine (TMB) into blue oxTMB. The other two free radicals (h + and ·OH) played minor roles during the catalytic reaction. Hg 2+ could integrate with S 2− to form HgS and the surface charges of O 2 were transferred to Hg 2+ to promote O 2 adsorption. DFT theoretical calculations highlight that the main reasons for the enhancing effect of Hg 2+ on color development results from electron transfer and increased adsorption energy of O 2 molecules onto the surface of NSC/Co 6 Ni 3 S 8 . By employing the oxidase-like activity of NSC/Co 6 Ni 3 S 8 and Hg 2+ -triggered promoting effect, a colorimetric sensing platform was established for Hg 2+ assay with a linear range of 10–200 μg/L and detection limit of 3 μg/L. Through integration with a smartphone-based APP “Thing Identify” software, a visual colorimetric assay for Hg 2+ was constructed with a detection limit of 5 μg/L. Compared to the data detected by the mercury vapor meter, the relative recoveries of 92.4–108.1% evidenced the higher accuracy of this smartphone-based visual detection. Overall, the NSC/Co 6 Ni 3 S 8 -based colorimetric assay is convenient, rapid, and visual, and can be applied for routine monitoring of Hg 2+ in real-world waters under outdoor conditions.
研究领域:
分析化学
·
环境科学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）