Analytical Methods
·
2025
·
IF 2.6
Fengyun Zhang,Yaoqi Wei,Yaping Zhang,Huanhuan Zhang,Lei Jiang,Xiangfei Liang
The 2-(5-bromo-2-pyridylazo)-5-diethylaminophenol (Br-PADAP) spectrophotometric method is widely employed for uranium (U(VI)) determination due to its high sensitivity. However, conventional protocols require toxic sodium fluoride as both a masking agent and a stabilizer, posing environmental and operational risks. To address this limitation, we developed an eco-friendly alternative utilizing low-toxicity sodium dodecyl sulfonate (SDS) for dual-functional masking and stabilization. Critical parameters including pH, reagent stoichiometry, and reaction kinetics were systematically optimized. The Br-PADAP-U(VI) complex exhibited maximum absorbance at 576 nm (ε = 4.33 × 104 L mol−1 cm−1), with rapid color development and stable signal retention (>4 h) under ambient conditions. Linear Beer–Lambert compliance was observed within 0.0–4.0 μg per mL U(VI), yielding a Sandell sensitivity of 0.0055 μg cm−2. Notably, the method demonstrated exceptional tolerance toward coexisting ions (Na+, K+, Ca2+, Mg2+, Fe3+, Cl−, CO32−, and Cr2O72−). Validation via spike-recovery tests in environmental water samples achieved 96.4–104% recoveries (0.5%< RSD <2%), confirming the reliability for field-deployable U(VI) quantification with advantages of simplicity, rapidity, and accuracy.
研究领域:
分析化学
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环境化学
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光谱学
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核化学
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水质监测
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DOI
PubMed
高影响因子
ACS Omega
·
2025
·
IF 4.3
Hongjing Yang,Zhipeng Zang,Zhen Wang,Kai Song,Yunzhen Gao,Jinbin Wei,Lu Han,Jiandong Zhang,Chen Liu
Starch and cellulose are pivotal determinants of tobacco quality. In this work, starch and cellulose in flue-cured tobacco leaf were extracted through a sequential extraction process. First, an aqueous solution of tobacco leaves was prepared, and starch was extracted by boiling the leaves in water. The precipitate was separated, and the cellulose within it was hydrolyzed using a sulfuric acid solution. Then, starch content was quantified by a colorimetric reaction with iodine solution, while cellulose content was determined by using anthrone as a chromogenic agent. The reaction conditions were optimized to ensure accuracy, and the recovery rate and relative standard deviation (RSD) were calculated under these optimum conditions. The recovery rates for starch and cellulose in the tobacco samples ranged from 90 to 105%, with the RSD of interday and intraday determination being less than 5%. These results demonstrate that the proposed method is both sensitive and reproducible, making it suitable for the rapid and precise quantification of starch and cellulose in tobacco samples.
研究领域:
植物学
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分析化学
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DOI
PubMed
高影响因子
Analytical Methods
·
2014
·Vol. 6
·期 19
·7973-7977
·
IF 1.938
Yingfeng Qin,Liangliang Zhang,Gaojie Ye,Shulin Zhao
A homogeneous label-free colorimetric sensing strategy for bleomycin (BLM) assay was developed on the basis of BLM enhanced Fe(II)–H2O2–ABTS reaction. Fe(II) exhibits a catalytic effect on H2O2-mediated oxidation of ABTS by Fenton chemistry. BLM·Fe(II) complex possessed a significantly higher catalytic ability than Fe(II) probably because of the further formation of more active “activated oxo-iron-bleomycin complex”. The present method was convenient without any complicated chemical synthesis, modification, or tedious experimental procedures. Analysis results can be seen with the naked eye and monitored by UV-vis spectra. The peak absorbance exhibits a linear relationship with BLM concentrations from 25 nM to 1 μM, and a detection limit of 16 nM can be achieved. The designed colorimetric strategy may provide a promising alternative for BLM detection in clinical samples.
研究领域:
分析化学
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生物医学
·
临床检验
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DOI
高影响因子
MATERIALS LETTERS
·
2020
·
IF 3.204
Shengrui Zhang,Qin Wang,Zhifeng Liu,Jin Liu,Jiufu Lu
A colorimetric turn-on probe was developed for the selective naked-eye determination of thiophenols by using yellow-emissive carbon dots (Y-CDs) as the colorimetric reporter and 2, 4-dinitrobenzenesulfonamide (DNBS) group as the recognition unit. The surfaces of Y-CDs were covalently coated with 2, 4-dinitrobenzenesulfonyl chloride to afford the 2, 4-dinitrobenzene- functionalized carbon dots ( CD-DNBS ) as a novel probe for detecting thiophenols. CD-DNBS was nearly colorless. Upon addition of thiophenol , the DNBS moiety of CD-DNBS could be readily cleaved via nucleophilic substitution reactions, leading to a dramatic increase in absorbance at 400 nm. The established detection probe displays a high selectivity towards thiophenol over biothiols and other biologically related species.
研究领域:
分析化学
·
生物化学
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2012
·
IF 4.555
Ruimin Li,Cen Xiong,Zhiyou Xiao,Liansheng Ling
A novel colorimetric method for detection of cholesterol was developed with hemin-G-quadruplex DNAzyme by transducing oxidation of cholesterol into the color change of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS 2− ). Oligonucleotide 5′-GTGGGTAGGGCGGGTTGG-3′ (Oligo-1) formed G-quadruplex structure in the presence of K + , it acted as a horseradish peroxidase (HRP) mimicking DNAzyme when binding hemin and catalyzed the oxidation of colorless ABTS 2− to green ABTS − by H 2 O 2 , which was produced by the reaction of cholesterol and oxygen that catalyzed by cholesterol oxidase. Therefore, the oxidation of cholesterol could be transduced into the color change of ABTS 2− by combining these two reactions. Under the optimum conditions, the absorbance was proportional to the concentration of cholesterol over the range of 1.0–30 μM, with a linear regression equation of A = 0.362 + 0.0256 C ( C : μM, R = 0.998) and a detection limit of 0.10 μM (3 σ /slope). Moreover, the practicability of the assay in the detection of cholesterol in human serum was studied as well.
研究领域:
生物化学
·
医学诊断
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产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2021
·Vol. 26
·期 9
·2738
·
IF 4.412
Xiang Lai,Yu Han,Jie Zhang,Jinyu Zhang,Weifeng Lin,Zhiwei Liu,Longgang Wang
The sensitive and selective detection of dopamine (DA) is very important for the early diagnosis of DA-related diseases. In this study, we reported the colorimetric detection of DA usingGanoderma lucidumpolysaccharide (GLP) stabilized platinum nanoclusters (Ptn-GLP NCs). When Pt600-GLP NCs was added, 3,3’,5,5’-tetramethylbenzidine (TMB) was rapidly catalyzed and oxidized to blue oxTMB, indicating the peroxidase-like activity of Pt600-GLP NCs. The catalytic reaction on the substrate TMB followed the Michaelis-Menton kinetics with the ping-pong mechanism. The mechanism of the colorimetric reaction was mainly due to the formation of hydroxyl radical (•OH). Furthermore, the catalytic reaction of Pt600-GLP NCs was used in the colorimetric detection of DA. The linear range for DA was 1–100 μM and the detection limit was 0.66 μM. The sensitive detection of DA using Pt-GLP NCs with peroxidase-like activity offers a simple and practical method that may have great potential applications in the biotechnology field.Keywords:dopamine;detection;platinum;colorimetric;Ganoderma lucidumpolysaccharide
研究领域:
生物技术
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医学诊断
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DOI
PubMed
高影响因子
RSC Advances
·
2019
·Vol. 9
·期 48
·28248-28252
·
IF 3.049
Yumin Leng,Fang Hu,Chunhua Ma,Chenxi Du,Linfeng Ma,Jiao Xu,Qiyuan Lin,Zhipei Sang,Zhiwen Lu
A simple, rapid, sensitive, selective and label-free method is presented for the colorimetric determination of lincomycin (Lin) by using HAuCl4 and NaOH. Upon the addition of Lin, the mixture of HAuCl4 and NaOH shows a color change from colorless to blue (or dark blue). The limit of colorimetric detection is as low as 1 μM, observed both in Milli-Q water and real samples. The selectivity of Lin detection is excellent compared with 9 other common antibiotics. On the basis of the “three-color” principle of Thomas Young, we extracted the red, green and blue (RGB) alterations of the sensor in the absence and presence of different concentrations of Lin. The color changes are quantitatively illustrated by the total Euclidean distances (EDs = [ΔR2 + ΔG2 + ΔB2]1/2). The good linear relationship between the EDs and Lin concentration is used for the quantitative assay of Lin. The developed method demonstrates great potential for the detection of Lin in environmental water and milk.
研究领域:
分析化学
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环境科学
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食品科学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2014
·Vol. 5
·期 5
·3888-3893
·
IF 3.708
Qian Tang,Hong-Mei Nie,Cheng-Bin Gong,Hua-Dong Liu,Kun Xiao
This study aimed to develop a recyclable, fluorescent, and colorimetric sensor for fluoride anion (F−) in aqueous media with fast response, good selectivity, and high sensitivity. A crosslinked polymer CpA1T5 was designed and synthesized as a sensor using (E)-1-allyl-4-((4-hydroxylphenyl)vinyl) quinolinium bromide as functional monomer, trimethylolpropane trimethacrylate as crosslinker, and azobisisobutyronitrile as initiator. When F− was introduced to the CpA1T5 suspension in deionized water, a change in color from gray to yellow and fluorescence enhancement accompanied with a redshift from 531 nm to 540 nm of fluorescence emission were immediately observed. This F− sensor can be recycled many times, making it cost efficient and beneficial to the environment. Analytical application of CpA1T5 to measure F− concentration in two commercial mouthwash samples was explored with good accuracy.
研究领域:
分析化学
·
材料科学
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DOI
高影响因子
SENSORS
·
2018
·Vol. 18
·期 10
·3252
·
IF 2.475
Feng Zhang,Xiaojie Wang,Xu Jie,Weili Wei
Fatty acids (FAs) are of interest to the areas of food science and medicine because they are important dietary sources of fuel for animals and play important roles in many biological processes. The health effects of FAs are different due to the diversity of olefinic bonds in the alkyl chains including number, position and configuration. However, the discrimination of FAs is difficult from a chemical sensing perspective due to the lack of diversity in terms of functional groups. Until now, only a few chemosensors have been developed for selective sensing of FAs based on their overall shape, however they are still limited in discrimination of FAs with subtle structural differences, moreover, they cannot be used for rapid and in situ inspections. Herein, for the first time, we designed a test paper for in situ colorimetric inspection for FAs based on the combination of the highly selective binding of Ag+to olefinic bonds and Ag+mediated color variation of 3,3′,5,5′,-tetramethylbenzidine. As a result, the sensor exhibited high sensitivity and good selectivity for five FAs with subtle structural differences. Furthermore, our method described herein was successfully applied to monitor the structural variations of FAs and quality changes in mixture edible hot pot oils with heat treatment in time course. Hence, the test paper presented herein holds great potential in the inspection of fats and edible oils in food industries.Keywords:test paper;colorimetric sensor;fatty acid;edible oil
研究领域:
食品科学
·
医学
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Shuangshou Wang,Haili Wang,Yuwen Ding,Wenzhi Li,Hongrui Gao,Zongpeng Ding,Peng Lin,Jing Gu,Mingfu Ye,Tingxuan Yan,Hongmei Chen,Jin Ye
Herein, a robust instrument-free point-of-care tests (POCTs) strategy was proposed for rapid naked-eye detection of artificial food colorants (AFCs) using filter paper as a sampling and response device. Indicator papers were prepared by one-step polycondensation of silane coupling agents onto glass microfiber filter papers, then colorimetric cards with fine color mappings to AFCs concentration gradients were handily fabricated by AFCs extraction with indicator papers via electrostatic interaction and hydrophobic effect. The lowest concentration for AFCs visual detection was as low as 10 −6 M and the whole POCTs process could be finished within 1 min. Furtherly, color information (RGB and gray-scale values) of AFCs-stained indicator papers and colorimetric cards from smartphone APP was used to reconstruct digitized colorimetry and reinforce its validity by mitigating the possible errors from subjective judgement on colors. Finally, such a dual-mode POCTs strategy was experimentally proved to be highly reliable for rapid visual detection of three AFCs (brilliant blue, Allura red, and sunset yellow) in real drinks by contrast with several instrumental methods like spectrophotometry, HPLC and MS. Due to its fine portability, speediness, low cost and user-friendliness, this strategy paves a promising way for AFCs-related food safety control.
研究领域:
食品科学
·
分析化学
·
即时检测
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Chenjing Xu,Jinpo Xie,Lian Yu,Bin Shu,Xiaogang Liu,Siping Chen,Qinglan Li,Shaohai Qi,Suqing Zhao
Vibrio vulnificus infection caused by contaminated aquatic products and seawater can lead to severe disease and high mortality. The development of a rapid and sensitive detection method for Vibrio vulnificus is vital to effectively prevent infection in advance. In this study, CeO 2 @PtRu with high peroxidase activity was used to construct a colorimetric immunoassay for Vibrio vulnificus detection by conjugating polyclonal antibodies via the biotin-streptavidin system. The developed colorimetric biosensor for Vibrio vulnificus demonstrated rapid operability and good sensitivity with a detection range from 10 4 CFU/mL to 10 9 CFU/mL, and the limit of detection (LOD) is 193 CFU/mL. Moreover, the colorimetric biosensor showed excellent specificity and good recoveries from 98.70% to 102.10% with RSD < 7.45% for spiked real samples. This novel CeO 2 @PtRu-based colorimetric biosensor has great application potential for the sensitive detection of Vibrio vulnificus in seafood.
研究领域:
微生物学
·
免疫学
·
生物传感技术
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 12
·1-7
·
IF 5.479
Leng Yumin,Fu Yu,Lu Zhiwen,Sang Zhipei,Liu Kecheng,Du Chenxi,Ma Linfeng
A general approach is presented for synthesis of multicolored gold nanoparticles (GNPs) by Au(I)-mediated generation of interlocking rings in proteins and antibiotics. The Au(I) ions are shuttled from proteins to antibiotics, and this causes the formation of interlocking rings. The multicolored GNPs of different sizes were synthesized in the rings by using the rapid nucleation method. To take the unique colors of GNPs, a functional array was designed for the colorimetric determination and discrimination of antibiotics, specifically of amoxicillin, chlortetracycline, erythromycin, spiramycin, neomycin, thiamphenicol, gentamycin and lincomycin. The method is based on the “three color” (RGB) principle. The color response patterns are characteristic for each antibiotic and can be quantitatively differentiated by statistical techniques. The limits of detection (LOD, at S/ N = 3) for spiramycin (Sp) have been calculated to be 0.18 μM and 0.10 μM in water and milk, respectively. The good linear range (from 0.3 to 3.5 μM) has been used for the quantitative assay of Sp in a certified reference material.
Schematic presentation of gold nanoparticles (GNPs) synthesis via formation of interlocking rings in protein and antibiotics. The Au(I) ions mediate protein and antibiotics to be interlocking rings, which are quickly fixed via microwave reaction. The GNPs are synthesized and assembled in the rings.
研究领域:
生物医学工程
·
纳米技术
·
药物分析
·
生物传感
·
食品安全
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DOI
高影响因子
危险品化学品经营许可证（带存储）