ELECTROPHORESIS
·
2014
·
IF 3.161
Ying Feng,Tingting Wang,Zhengjin Jiang,Bezhan Chankvetadze,Jacques Crommen
A series of eight chiral β-blocker drugs, acebutolol, atenolol, carazolol, carteolol, carvedilol, propranolol, sotalol, and talinolol, have been enantioseparated using two single-component anionic β-CD derivatives, namely heptakis (2,3-di- O -methyl-6-sulfo)-β-CD (HDMS-β-CD) and heptakis (2,3-di- O -acetyl-6-sulfo)-β-CD (HDAS-β-CD), in aqueous CE and NACE. The influence of the nature of substituents (methyl or acetyl) in positions 2 and 3 on the CD derivatives and of the electrophoretic medium (water or methanol) on the enantioselectivity and enantiomer affinity pattern (EAP) of these structurally related compounds was systematically studied. All eight β-blockers could be enantioseparated at least partially in the four CE systems, except sotalol with HDMS-β-CD in NACE. In general, lower affinity and enantioselectivity were obtained in the presence of HDMS-β-CD compared to HDAS-β-CD. Reversals of EAPs were observed for all compounds. EAPs toward these two CDs were found to be opposite to each other in NACE for all compounds except carvedilol and in aqueous CE for atenolol, carteolol, talinolol, and sotalol. It is particularly noteworthy that opposite EAPs were also observed using the same CD derivative when the aqueous BGE was replaced with the methanolic one: for carazolol, carvedilol, and propranolol in the presence of HDMS-β-CD and for acebutolol and carvedilol with HDAS-β-CD.
研究领域:
药物化学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of the Taiwan Institute of Chemical Engineers
·
2021
·
IF 5.876
Jiaxin Huang,Yatian Zhang,Chunxian Tao,Shanni Li,Qingxiang You,Dawei Zhang,Zhenqing Li,Yoshinori Yamaguchi
Background Capillary electrophoresis (CE) is an effective method for protein analysis. Although previous researchers have proved that the separation performance of large DNA molecules was improved by pulsed field capillary electrophoresis (PFCE), there is lack of research about proteins separation by PFCE. Methods Herein, we systematically investigated the separation performance of proteins sized from 44 kDa to 200 kDa under square wave and inversion electric field, respectively. Findings Results showed that for pulsed electric field with same forward and backward time duration, there was a resonance frequency for protein molecule re-orientation and applied frequency, and the apparent mobility ( μ ) was the lowest at the resonance frequency. μ was positively correlated to modulation depth ( M ) if M was below 200%, while μ was negatively correlated to M if M was higher than 200%. For CE with same forward and backward voltage, it demonstrated that μ dropped as M went up, but the separation performance for proteins with high molecular mass improved. Through PFCE, albumin and α- globulins in human serum were also resolved within 7 min. Such a work may shed new light on the analysis of proteins by PFCE.
研究领域:
分析化学
·
生物技术
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Xin-Ran Ma,Yi-Wen Wu,Qian-Xue Shen,Yan-Hua Jiao,Jun Cao,Li-Hong Ye
One-step admicelle to cyclodextrin sweeping (ACDS) method in capillary electrophoresis (CE) has been developed for the separation and preconcentration of toxic aristolochic acids (AAs) by applying cetyltrimethylammonium chloride (CTAC) between the critical micelle concentration and critical surface aggregation concentration. The analytes were captured into the CTAC admicelles, after the more stable inclusion complexes were formed, the release and preconcentration of the analytes could be realized. The CE enrichment parameters, such as the types of cationic surfactants and cyclodextrins (CDs), the concentration of cationic surfactants, disodium tetraborate decahydrate (borax) and CDs, the volume ratio of acetonitrile (ACN) in background electrolyte (BGE) and the sample injection time were optimized systemically. The optimal BGE was composed of 20 mM borax, 10 mM γ-CD and 10 % (v/v) ACN, the sample matrix consisted of 0.2 mM CTAC, 20 mM borax and five analytes. The limit of detection of this method was 3–6 ng/mL, and good linearity values (R 2 ) were obtained for five AAs in the range of 0.997–0.999. Applying optimized conditions, the enrichment efficiencies of five AAs were enhanced with the sensitivity enrichment factors of 30–68. The sweeping approach was successfully applied to the determination of A, B, Ⅶ a , C and D in Aristolochia and Asarum samples, which could provide a potential in the enrichment of the other toxic compounds.
研究领域:
分析化学
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2017
·
IF 3.981
Chen Zhao,Yan Yang,Linfang Wei,Qinghua Guo,Hao Fang,Shaolin Chen,Qiang Hua
Yarrowia lipolytica is an oleaginous yeast with promise in producing terpenoids such as lycopene . Though methods for analyzing primary metabolic intermediates have been established, further work is needed to better analyze nucleotides and coenzymes . Here, we presented an optimized method for the separation of nucleotides and coenzymes in Y. lipolytica using the capillary electrophoresis. The separation of twelve metabolites including four coenzymes, five nucleotides and three nucleosides was achieved within 32 min using a voltage of 15 kV and 70 mM sodium carbonate/hydrogencarbonate buffer with 1.0% β -CD at pH 10. The results show that the concentrations of adenosine triphosphate and nicotinamide adenine dinucleotide phosphate changed significantly between lycopene producing strain and the control, indicating that these two metabolites may be closely related with lycopene production. The optimized method provides a useful approach for future metabolic analysis of fermentation process as well as industrial strain improvement.
研究领域:
生物化学
·
代谢工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ELECTROPHORESIS
·
2020
·Vol. 41
·期 15
·1273-1279
·
IF 3.081
Litao Zhao,Fanghong Luo,Anting Wang,Jing Zhang,Yuanhang Wang,Liangtao Zhao,Zhaoyan Wang,Qiaosheng Pu
Mutations in the potassium channel genes may be linked to the development of epilepsy and affect the blood potassium levels. Therefore, accurate determination of potassium in the blood will be critical to diagnose the cause of epilepsy. CE is a competent technique for the fast detection of multiple ions, but complicated matrices of a blood sample may cause significant variation of migration times and the peak shape. In this work, a procedure for rapid stabilization of the capillary inner surface through preflushing of a blood sample was employed. The process takes only 40 min for a capillary and then it can be used for more than 2 weeks. No pretreatment of the blood sample or other surface modification of the capillary is needed for the analysis. The RSDs of the migration time and peak area were reduced to 1.5 and 5.1% from 12.6 and 14.5%, respectively. The proposed method has been successfully applied to the determination of the potassium contents in the blood sample of patients with epilepsy at different stages. The recoveries of potassium ions in these blood samples are in a range from 86.5 to 104.5%.
研究领域:
医学诊断
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
STEROIDS
·
2020
·
IF 1.948
Bairen Du,Jingjing Zhang,Yanjie Dong,Junwei Wang,Longwen Lei,Rengfei Shi
A novel method for determining the testosterone/epitestosterone concentration ratio in human urine was established by capillary electrophoresis with diode-array detector. The urine samples were firstly purified by the solid extraction. The optimal experimental conditions were: running buffer pH = 4.74, 15.0 mmol L −1 HAc-NaAc, separation voltage 25 kV, temperature 25 °C, sample injection pressure 3.43 × 10 3 Pa, and duration 10 s. The testosterone and epitestosterone linear range were determined as 8.0–960.0 ng mL −1 , respectively. The testosterone and epitestosterone detection limits were determined as 4.6 and 4.5 ng mL −1 , respectively. The relative standard deviation was less than 0.36%.
研究领域:
分析化学
·
医学检验
·
运动科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 20
·6987
·
IF 4.927
Yixuan Fan,Runze Yu,Yongfeng Chen,Yufeng Sun,Geoffrey I. N. Waterhouse,Zhixiang Xu
In this study, a sensitive capillary electrophoresis (CE) method based on molecularly imprinted solid-phase extraction (MISPE) was proposed to determine histamine in foods. A molecularly imprinted polymer (MIP) synthesized by bulk polymerization was used as the MISPE adsorbent for the selective extraction of histamine. Under the optimal conditions, the MISPE-CE method possessed good linearity for histamine detection in the concentration range of 0.1–100.0 μg/L. The limit of detection and limit of quantification of the method were calculated to be 0.087 μg/L and 0.29 μg/L, respectively. The histamine in spiked rice vinegar and liquor samples were detected by the developed method with recoveries of 92.63–111.00%. The histamine contents in fish, prawn, pork, chicken breast and soy sauce samples were determined using the developed method and a high-performance liquid chromatography method, with no significant difference found between the two methods.Keywords:histamine;molecular imprinting technology;solid-phase extraction;capillary electrophoresis
研究领域:
分析化学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2019
·
IF 2.983
Zhenqun Li,Jia Wu,Li Jia
Detection of amino acids (AAs) in blood is helpful to diagnose some diseases. In this work, a method is developed for determination of trace amounts of AAs in bovine blood by combining solid phase extraction (SPE) and capillary electrophoresis (CE). Zeolitic imidazolate framework-8 (ZIF-8) nanoparticles are prepared and used as adsorbents to simultaneously extract three AAs (tryptophan, tyrosine, phenylalanine). Under the optimum extraction conditions, the extraction efficiencies of ZIF-8 for AAs are 95.1% (tryptophan), 91.1% (tyrosine), and 90.1% (phenylalanine), respectively. Interestingly, ZIF-8 demonstrates good extraction ability for AAs in high concentration of acetonitrile (ACN) aqueous solutions. Thus, they can be directly used to extract AAs from the deproteinized blood sample using ACN. An α-cyclodextrin-mediated cation selective exhaustive injection-sweeping CE method is developed for analysis of the extracted AAs. The detection limits for AAs range from 0.13 to 0.37 μg mL −1 using the SPE/CE method. Standard addition method is used to evaluate the feasibility of the method in bovine blood samples. The standard addition curves demonstrate good linearity with determination coefficient > 0.9912.
研究领域:
分析化学
·
医学诊断
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DYES AND PIGMENTS
·
2011
·
IF 2.635
Haiyan Li,Zhongduo Xiong,Xibin Dai,Qinqfu Zeng
In order to investigate the impact of perspiration on photo-induced chemical reaction of azo dyes and the carcinogenic aromatic amine products produced from the reaction, we have carried out experimental studies on the photochemical reaction of C.I. Reactive Red 2 mixed with American Association of Textile Chemists and Colorists（AATCC）standard artificial perspiration. UV–vis spectroscopic technique was employed to monitor the reaction processes, and the reaction products were analyzed by hollow fiber protected liquid-liquid-liquid phase micro-extraction with capillary electrophoresis（HF-LLLME-CE). The results showed that perspiration had remarkable influence on the photochemical reaction of azo dyes. Aromtic amines formed during the photochemical process as a result of reduction of azo dyes by organic components in perspiration. The HF-LLLME-CE methodology was validated in analyzing aromatic amines produced from the photochemical degradation of azo dye C.I. Reactive Red 2 and C.I. Acid Red 35 mixed with artificial perspiration.
研究领域:
分析化学
·
毒理学
·
环境科学
✓ 已验证
DOI
高影响因子
FERROELECTRICS
·
2024
·
IF 0.6
Xi-Jie Wang,Shuai Lv,Hai-Lin Cong,You-Qing Shen,Qing-Hai Niu,Bing Yu
Functionally modified coated capillary is essential for reducing the nonspecific interaction between samples and analytical instruments and improving detection sensitivity and analytical efficiency. Here, we used perfluorooctanoic acid and diazo resin as raw materials, and bonded them to the inner wall of the capillary through the electrostatic interaction of the coupling agent to construct a self-assembled coating tube with anti-fouling properties. The dynamic adsorption test of fluorescent protein confirmed that the coating had obvious anti-protein adsorption effect. The coating was used for capillary electrophoresis (CE) analysis of four mixed protein standard samples and egg white samples. The excellent protein separation spectrum confirmed the application value of the coating.
研究领域:
分析化学
·
蛋白质组学
✓ 已验证
产品关联
DOI
高影响因子
ELECTROPHORESIS
·
2012
·Vol. 34
·期 2
·277-283
·
IF 3.303
Libo Li,Hongwei Du,Huan Yu,Lei Xu,Tianyan You
CE coupled with amperometric detection method was developed using ionic liquid 1-ethyl-3-methylimidazolium tetrafluoroborate (EMImBF 4 ) as additive for the simultaneous detection of clenbuterol (CLB), terbutaline (TER), and ractopamine (RAC) in feed. The effects of detection potential, concentration of EMImBF 4 , pH, and concentration of the running buffer, separation voltage as well as injection time on the separation and detection of these three β -agonists were investigated in detail. Under the optimum conditions: the detection potential at 1.05 V, 50 mmol/L Tris-HAc at pH 8.0 with 0.6% (v/v) EMImBF 4 , electrokinetic injection 6 s at 16 kV and separation voltage at 16 kV, a baseline separation for these three analytes could be achieved within 11 min. Introduction of EMImBF 4 into the running buffer resulted in significant improvement in separation selectivity and enhancement in peak currents for those β -agonists, especially for TER and RAC, which could not be separated in the running buffer without additive. The method exhibited wide linear range with LOD (S/N = 3) of 2, 1, and 2 nmol/L for CLB, TER, and RAC, respectively. The precision was determined in both intraday ( n = 5) and interday ( n = 3) assays, and the RSDs for both migration time and peak current were less than 6%. The proposed method was also applied to analyze β -agonists in feed sample.
研究领域:
分析化学
·
兽医药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2020
·
IF 5.774
Qi Zhang,Siru Ren,Song Xue,Ang Li,Siyao Liu,Xiaodong Sun
In the field of separation science, the use of tartaric acid-based ionic liquids (TTILs) for chiral separation has not yet been reported. In this work, six novel mono- and di-tetraalkylammonium l -tartrate ILs (TAA- l -TTs) with different alkane chain length were evaluated for their potential as sole chiral selectors in capillary electrophoresis (CE). In comparison with conventional l -tartaric acid ( l -TT) chiral selector, these IL-type chiral selectors exhibit remarkably improved enantioseparation performance, which is even better than most previously reported TT ester-type chiral selectors. Major factors influencing CE separation were investigated, including the background electrolyte (BGE) composition, ILs concentration, type and proportion of organic modifier, buffer pH, etc. The results show that the l -TT-based IL-type chiral selectors prefer a weak acid buffer (150 mM boric acid at pH 4.5) with a high proportion of methanol (80%-90%). The best resolutions (Rs) were obtained when the IL concentration was set at 60 mM. We further carried out a series of comparative experiments and a molecular simulation study to explore the enantiorecognition mechanism of TTILs. The result indicates that the TAA- l -TTs cannot be considered as a simple combination of TAA salt and l -TT. The dissociation degree of TTILs may be an important factor influencing the enantiorecognition process.
研究领域:
分析化学
·
分离科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）