FOOD RESEARCH INTERNATIONAL
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2026
·
IF 8.8
Xiangrui Ren,Temirlan Khamiddolov,Li Niu,Qian Lin,Wenqing Zhao,Yìxiang Liu,Wenhao Li
This study systematically evaluated the effects of ultrasound-assisted vacuum freeze-drying (Ul-FD), variable-temperature and variable-speed hot air drying (VT/VS-HD), and modulated microwave drying (Mo-MD) on the quality of Osmanthus fragrans var. thunbergii ( O. fragrans ). The results indicated that Ul-FD best preserved chromatic attributes and cellular structure, retaining the highest levels of total phenolics (1.08 μg GAE/mg DW), total flavonoids (0.80 μg RE/mg DW), and antioxidant capacity (DPPH scavenging activity of 94.57%), significantly outperforming the other treatments. Mo-MD significantly reduced the drying time to 20 min and exhibited the fastest dynamic process of moisture migration and bound water transformation. HRLC-MS identified 82 phenolic compounds, with Ul-FD best preserving polyphenol diversity, Mo-MD enriching heat-resistant aglycones, and VT/VS-HD promoting the formation of small-molecular acids. PCA and PLS-DA identified 17 differential metabolites, demonstrating that drying methods significantly reshape the polyphenome. KEGG enrichment analysis indicated that the phenylpropanoid and flavonoid biosynthesis pathways were the main responsive routes. This study links the drying process to phenolic metabolism and bioactive quality. It identifies Ul-FD for high-value, Mo-MD for industrial, and VT/VS-HD for general applications, providing a basis for optimizing O. fragrans drying and product development.
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PubMed
高影响因子
Food Frontiers
·
2025
·
IF 6.9
Qinle Huang,Zhangtie Wang,Binhai Shi,Guoliang Jie,Songbai Liu,Fuli Nie,Fan Wang,Zhenjiang Zhou,Siyu Chen,Jianfu Shen,Baiyi Lu
Osmanthus fragrans extract (OFE) has significant potential for application in the beverage and cosmetic industries. However, the conventional extraction processes of OFE are affected by multiple factors, making it challenging to identify parameters that can be synergistically optimized for diverse qualities. This study aimed to establish a multi-objective optimization (MOO) method for the ultrasonic-assisted extraction of O. fragrans var. thunbergii. The effects of time, temperature, ultrasonic power, and solid-liquid ratio on yield, content of total phenylethanol glycosides, verbascoside and salidroside, and colorimetric parameters were compared using response surface methodology and machine learning algorithms. Multi-layer perceptron, XGBoost, and support vector regression were evaluated as base models for constructing an ensemble model, using the SHAP algorithm for interpretation. A MOO method based on BP-ANN and NSGA-II was developed to maximize the content of bioactive compounds in the extract while considering other quality indicators. The optimal conditions for the target quality attributes were determined to be a temperature of 54°C, an extraction time of 52 min, a solid-liquid ratio of 16 mL/g, and an ultrasonic power of 345 W. This study provides a novel approach that combines artificial intelligence and MOO for process optimization in the plant extraction industry.
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FOOD AND CHEMICAL TOXICOLOGY
·
2020
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IF 4.679
Fei Zhou,Weisu Huang,Tao Xu,Lipeng Wu,Qi Chen,Jiyu Peng,Xin Liu,Baiyi Lu
Acteoside is one of the most widespread phenylethanoid glycosides with pharmacological activities , including antioxidant, neuroprotective property, etc. However, its bioavailability is poor due to the low absorption and P-gp efflux. This study aimed to select food derived P-gp inhibitors for promoting the acteoside absorption and investigate whether the inhibitors could increase the bioavailability and stability of acteoside. Results showed that EGCG and quercetin significantly decreased the BL-to-AP efflux and promoted the AP-to-BL influx of acteoside across Caco-2 monolayers with optimum concentrations of 320 μM EGCG or 240 μM quercetin adding to 320 μM acteoside. EGCG increased the bioavailability of acteoside to 1.43-fold, but quercetin had no such effect. Further study showed that EGCG and quercetin had no effects on the storage and digestion stability of acteoside. This work revealed that EGCG could improve the acteoside absorption across the Caco-2 monolayers and enhance the bioavailability of acteoside in rats.
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CHEMICAL & PHARMACEUTICAL BULLETIN
·
2020
·Vol. 68
·期 5
·428-435
·
IF 1.416
Si-qi Wang,Xiao Yang,Yong-gang Zhang,En-bo Cai,Xiao-man Zheng,Yan Zhao,Ge Li,Mei Han,Li-min Yang
Ginseng (G) and Prepared Rehmannia Root (PRR) are commonly used in traditional Chinese medicine for blood supplementation. This study aimed to study G and PRR with different compatibility ratios changes in chemical composition and inhibition of cyclophosphamide-induced myelosuppression. HPLC was used to determine the chemical constituents of 13 ginsenosides, 5-hydroxymethylfurfural (5-HMF) and verbascoside in different proportions of G-PRR. Balb/c mice were injected intraperitoneally with cyclophosphamide (CTX) to induce bone marrow suppression. The effects of different proportions of G-PRR on peripheral blood, bone marrow nucleated cells, thymus and spleen index of myelosuppressed mice were analyzed. The results showed that the compatibility of G and PRR can promote the dissolution of ginsenosides, and the content of conventional ginsenosides decreased, and the content of rare ginsenosides increased. Different proportions of G-PRR increased the number of peripheral blood and bone marrow nucleated cells in cyclophosphamide-induced bone marrow suppression mice (p < 0.01), increased thymus index (p < 0.01), decreased spleen index (p < 0.01). Different proportions of G-PRR can improve the myelosuppression induced by cyclophosphamide in mice, and the combined effect of G-PRR is better than the single decoction of G and PRR. Among them, G-PRR 2 : 3 and G-PRR 1 : 2 were better than the other groups. These results indicate that different proportion of G-PRR can improve bone marrow suppression, and the combined decoction of G-PRR is better than the separate Decoction in improving bone marrow suppression. This improvement may be related to the changes of the substance basis and active ingredients of G-PRR.
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FOOD HYDROCOLLOIDS
·
2018
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IF 5.089
Fei Zhou,Tao Xu,Yajing Zhao,Huaxin Song,Liuquan Zhang,Xiaodan Wu,Baiyi Lu
The stability and bioavailability of acteoside were improved by encapsulating it with liposomes and chitosan. The liposomes were characterized through transmission electron microscopy , differential scanning calorimetry and Fourier transform infrared spectroscopy , and their particle size, zeta potential , encapsulation efficiency ( EE ), in vitro release, storage stability and oral bioavailability were tested. Results showed that the particles were nanosized and spherical. The average diameter, zeta potential, EE and relative bioavailability ( F rel ) of the acteoside liposome (Ac-Lip) were 78.49 ± 1.44 nm, −4.93 ± 0.79 mV, 81.06 ± 3.48% and 217.62%, respectively. These parameters increased to 92.77 ± 2.99 nm, +19.65 ± 0.90 mV, 88.10 ± 5.36% and 442.84%, respectively, after the surface of Ac-Lip was coated with 2 mg/mL of chitosan (CS-Ac-Lip). In contrast to Ac-Lip, the CS-Ac-Lip showed a reduced in vivo release rate and enhanced storage stability. The chitosan coating increased the diameter, zeta potential, EE , in vivo release time, stability and bioavailability of the Ac-Lip, and the chitosan–liposome is a promising delivery system for transporting acteoside or other bioactive components.
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Scientific Reports
·
2017
·Vol. 7
·期 1
·1-10
·
IF 4.259
Zhou Fei,Zhao Yajing,Li Maiquan,Xu Tao,Zhang Liuquan,Lu Baiyi,Wu Xiaodan,Ge Zhiwei
This study was aimed at investigating the chemical stability (the thermal, light and pH stability) of phenylethanoid glycosides (PhGs) in Osmanthus fragrans Lour. flowers, identifying the degradation products of acteoside and salidroside (major PhGs in O. fragrans flowers) by UPLC–QTOF–MS and studying the anti-hypoxia activity of PhGs after degradation. The degradation of PhGs followed first-order reaction kinetics, and the rate constant of acteoside (4.3 to 203.4 × 10 −3 day −1 ) was higher than that of salidroside (3.9 to 33.3 × 10 −3 day −1 ) in O. fragrans flowers. Salidroside was mainly hydrolyzed to tyrosol during storage, and the degradation products of acteoside were verbasoside, caffeic acid, isoacteoside, etc. In a model of cobalt chloride (CoCl 2 )-induced hypoxia in PC12 cells, the anti-hypoxia ability of PhGs decreased after degradation, which resulted from the reduction of PhGs contents. Particularly, caffeic acid exhibited stronger anti-hypoxia ability than acteoside and could slightly increase the anti-hypoxia ability of degraded acteoside. The results revealed that high temperature, high pH and light exposure caused PhGs degradation, and thus the anti-hypoxia ability of PhGs reduced.
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高影响因子
BIOMEDICAL CHROMATOGRAPHY
·
2025
·Vol. 39
·期 2
·e6065
·
IF 1.7
Yong Jiang,Wenjun Ji,Ying Lu,Qin Wang,Linwei Chen
The high mortality rate of chronic heart failure (CHF) makes it a primary battlefield in the field of cardiovascular diseases. Qiangxin Lishui Prescription (QLP) is a traditional Chinese medicine (TCM) prescription used clinically for treating CHF, but its underlying mechanism remains unclear. This study integrated plasma metabolomics, network pharmacology, and experimental validation to reveal the pharmacological effects of QLP and its potential mechanism of anti-CHF. Using ultra-high-performance liquid chromatography combined with quadrupole time-of-flight mass spectrometry (UPLC-Q/TOF-MS/MS), 119 absorbed prototype compounds of QLP were identified from rat plasma. By applying network pharmacology and molecular docking techniques, a QLP absorption components–target–CHF network was constructed. The IL6/JAK/STAT3 signaling pathway is likely critical to QLP's therapeutic effect on CHF. A CHF mouse model was established using aortic ligation surgery to investigate the regulation of the IL6/JAK/STAT3 pathway by QLP in CHF mice. Network pharmacology analysis and in vivo experimental data indicate that QLP alleviates myocardial injury and inflammatory response in CHF mice by modulating the IL6/JAK/STAT3 pathway, significantly improving cardiac function. This presents a promising therapeutic strategy for CHF treatment.
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Cellular and Molecular Bioengineering
·
2025
·
IF 5
Liu Junyu,Wang Hua,Zhang Caihua
Background Skin wound healing is a complex biological process involving cellular, molecular, and physiological events. Traditional treatments often fail to provide optimal outcomes, particularly for chronic wounds. Objectives This study aimed to develop a self-healing hydrogel loaded with Acteoside, a bioactive compound with antioxidant and anti-inflammatory properties, to enhance skin wound healing. Methods Using transcriptomic analysis, Rab31 was identified as a key target of Acteoside in regulating hair follicle stem cells (HFSCs). In vitro assays demonstrated that Acteoside promotes HFSC proliferation, migration, and differentiation by upregulating Rab31 expression. The self-healing hydrogel was prepared using quaternized chitosan derivatives, which exhibited excellent mechanical properties, antibacterial, and antioxidant activities. Results In vivo studies in a mouse model showed that Acteoside-loaded hydrogel significantly accelerated wound healing, promoting skin regeneration and improving wound closure. Conclusions This research highlights the potential of Acteoside-loaded self-healing hydrogels as an innovative therapeutic strategy for enhancing skin wound healing. By modulating HFSC activity, this hydrogel offers a promising solution for improving healing outcomes in challenging wound environments. Graphical Schematic representation of an injectable self-healing hydrogel loaded with the phenylethanoid compound acteoside for regulating the proliferation and differentiation of HFSCs to mediate the healing of skin wounds.
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Scientific Reports
·
2025
·Vol. 15
·期 1
·1-14
·
IF 3.9
Zhang Yuxi,Zhan Junbiao,Qiu Zhen,Tian Hao,Lei Shaoqing,Huang Qin,Xue Rui,Sun Qian,Xia Zhongyuan
Myocardial cold ischemia/reperfusion (I/R) injury is an inevitable consequence of heart transplantation, significantly affecting survival rates and therapeutic outcomes. Growth Differentiation Factor 15 (GDF15) has been shown to regulate GPX4-mediated ferroptosis, playing a critical role in mitigating I/R injury. Meanwhile, verbascoside (VB), an active compound extracted from the herbaceous plant, has demonstrated myocardial protective effects. In this study, heart transplantation was performed using a modified non-suture cuff technique, with VB administered at a dose of 20 mg/kg/day via intraperitoneal injection for 3 days in vivo. In vitro, cardiomyocytes were pretreated with 50 µg/ml VB for 24 h. VB treatment significantly reduced histopathological injury, decreased myocardial injury markers, and inhibited ferroptosis and oxidative stress during myocardial cold I/R injury in vivo. In vitro experiments further demonstrated that GDF15 alleviates ferroptosis induced by hypoxic reoxygenation by upregulating GPX4. Therefore, it is concluded that VB preconditioning can effectively reduce ferroptosis induced by myocardial cold I/R after heterotopic heart transplantation, possibly through up-regulation of GDF15/GPX4/SLC7A11 pathway.
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