Rongfei Zhang,Yingjun Cui,Meng Cheng,Yanli Guo,Xiangyou Wang,Juan Wang
Cinnamon essential oil-mesoporous silica nanoparticles (CEO-MSNPs)/potato starch films have better antifungal activity against molds commonly found in post-harvest white mushrooms. The antifungal effects of CEO-MSNPs against Mucor sp. FJ09 species and Mucor circinelloides CNRMA 03.0371 were investigated. The antifungal mechanisms of CEO-MSNPs against molds were elucidated. Results showed that after adding CEO-MSNPs at the minimum inhibitory concentration level, there were obvious changes in membrane permeability, reactive oxygen species (ROS) content, cell component leakage, cell component leakage, soluble sugar, and malondialdehyde (MDA) content. The scanning electron microscope assay showed that CEO-MSNPs effectively destroyed the mycelial morphology of Mucor sp. FJ09 species and Mucor circinelloides CNRMA 03.0371, and interfered with their normal growth. Notably, CEO-MSNPs could be in the up-regulation of nox1 and nox2 genes of Mucor sp. FJ09 species and Mucor circinelloides CNRMA 03.0371 to generate a large number of reactive oxygen species, thereby inhibiting spore germination and growth. The results of this study could provide a reference for screening key targets of drug resistance of molds in post-harvest white mushrooms.
Xuan Zhu,Yi-Fan Zhao,Hong-Jian Wen,Yu Lu,Shuai You,Richard Ansah Herman,Jun Wang
Silkworm pupae, by-product of sericulture industry, is massively discarded. The degradation rate of silkworm pupae protein is critical to further employment, which reduces the impact of waste on the environment. Herein, magnetic Janus mesoporous silica nanoparticles immobilized proteinase K mutant T206M and Mucor circinelloides aspartic protease were employed in the co-degradation. The thermostability of T206M improved by enhancing structural rigidity ( t 1/2 by 30 min and T 50 by 5 °C), prompting the degradation efficiency. At 65 °C and pH 7, degradation rate reached the highest of 61.7%, which improved by 26% compared with single free protease degradation. Besides, the immobilized protease is easy to separate and reuse, which maintains 50% activity after 10 recycles. Therefore, immobilized protease co-degradation was first applied to the development and utilization of silkworm pupae resulting in the release of promising antioxidant properties and reduces the environmental impact by utilizing a natural and renewable resource.
危险品化学品经营许可证（带存储）