PeerJ
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2019
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IF 2.353
Hua Yang,Yan-Lin Liu,Yuan-Yuan Tao,Wei Yang,Chun-Ping Yang,Jing Zhang,Li-Zhi Qian,Hao Liu,Zhi-Yong Wang
The bamboo snout beetle Cyrtotrachelus buqueti is a widely distributed wood-boring pest found in China, and its larvae cause significant economic losses because this beetle targets a wide range of host plants. A potential pest management measure of this beetle involves regulating olfactory chemoreceptors. In the process of olfactory recognition, pheromone-binding proteins (PBPs) play an important role. Homology modeling and molecular docking were conducted in this study for the interaction between CbuqPBP1 and dibutyl phthalate to better understand the relationship between PBP structures and their ligands. Site-directed mutagenesis and binding experiments were combined to identify the binding sites of CbuqPBP1 and to explore its ligand-binding mechanism. The 3D structural model of CbuqPBP1 has six a-helices. Five of these a-helices adopt an antiparallel arrangement to form an internal ligand-binding pocket. When docking dibutyl phthalate within the active site of CbuqPBP1, a CH- π interaction between the benzene ring of dibutyl phthalate and Phe69 was observed, and a weak hydrogen bond formed between the ester carbonyl oxygen and His53. Thus, Phe69 and His53 are predicted to be important residues of CbuqPBP1 involved in ligand recognition. Site-directed mutagenesis and fluorescence assays with a His53Ala CbuqPBP1 mutant showed no affinity toward ligands. Mutation of Phe69 only affected binding of CbuqPBP1 to cedar camphor. Thus, His53 (Between α2 and α3) of CbuqPBP1 appears to be a key binding site residue, and Phe69 (Located at α3) is a very important binding site for particular ligand interactions.
研究领域:
生物信息学
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生化分析
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昆虫学
✓ 已验证
DOI
PubMed
高影响因子
Frontiers in Physiology
·
2022
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IF 4.755
Yin Haichen,Li Wenjing,Xu Min,Xu Dong,Wan Peng
The mirid bugs Apolygus lucorum and Adelphocoris suturalis are considered serious pests of many crops in China, the host plant recognition of these pests remains unclear. The current study investigated the vital odor cues of two mirid bugs and evaluated the role of olfactory recognition in host recognition. The GC-EAD response of mirid bugs to volatiles of their host plant Phaseolus vulgaris was tested. Tetradecane, 2-propyl-1-pentanol, and dodecanal elicited strong EAG responses by mirid bugs and were tested with field experiments. The results indicated tetradecane was significantly more attractive than other attractants, yielding 30.33 ± 2.19 mirid bugs trapped during 7 days. The selected response rates to tetradecane were above 60%, which was most attractive to female A. lucorum at 1.5 mg/ml. Among seven tetradecane derivatives, tetradecane and tetradecanoic acid were the most potent attractants to A. lucorum and A. suturalis. Tetradecane was present in the volatiles of 10 common hosts, and their difference in relative content was significant. The presence of tetradecane seemed relevant to the olfactory response intensity of two mirid bugs towards the different host plants. The artificial supplement of tetradecane increased the attractive effect of host plants. These results suggested that tetradecane plays a vital role in the olfactory selection by two mirid bugs, and it can be made into field baits as a novel ecological strategy to manage these pests with widely reported pesticide resistance. However, results suggested host recognition is not entirely dependent on odor cues. We demonstrated that A. suturalis and A. lucorum adults have similar olfactory recognition mechanisms to their hosts in long-distance host selection. While, the differences in host plant selection between the two pests should occur in close range due to differences in gustatory or tactile sensory organs of A. lucorum and A. suturalis.
研究领域:
昆虫学
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生态学
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农业科学
✓ 已验证
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
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IF 15.1
Jiawang Hu,Dong Liu,Xuanjie Xia,Bin Wang,Donglei Pan,Yifan Cheng,Yuan Lu
Human identification is crucial in many fields. However, it is challenging to achieve instant and accurate human identification through body odors. In this work, an in-situ growth strategy was utilized to establish a gas-sensitive nanocomposite material library with MXene and perovskite . A plug-and-play bionic sensor array module based on the material library was manufactured and assembled into an instant detection platform (IDP). Machine learning (ML) algorithms were introduced to assist IDP in identifying human odors. Due to its unique Schottky Barrier structure, the material library exhibited higher performance and responded 37 % ∼ 70 % higher than initial MXene. IDP was applied to detect the odors of volunteers’ breaths and clothes with an accuracy rate of 69.2 % and 51.1 %, respectively, with the assistance of ML. In all, an instant, convenient, and accurate human identification prototype machine was fabricated, providing a general solution for more complex application scenarios.
研究领域:
材料科学
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人工智能
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生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
WATER RESEARCH
·
2023
·
IF 12.8
Lin Huang,Weixia Zhang,Difei Tong,Lingzheng Lu,Weishang Zhou,Dandan Tian,Guangxu Liu,Wei Shi
Recently, increased production and consumption of disinfectants such as triclosan (TCS) and triclocarban (TCC) have led to massive pollution of the environment, which draws global concern over the potential risk to aquatic organisms. However, the olfactory toxicity of disinfectants in fish remains elusive to date. In the present study, the impact of TCS and TCC on the olfactory capacity of goldfish was assessed by neurophysiological and behavioral approaches. As shown by the reduced distribution shifts toward amino acid stimuli and hampered electro-olfactogram responses, our results demonstrated that TCS/TCC treatment would cause deterioration of the olfactory ability of goldfish. Our further analysis found that TCS/TCC exposure suppressed the expression of olfactory G protein-coupled receptors in the olfactory epithelium, restricted the transformation of odorant stimulation into electrical responses by disturbing the cAMP signaling pathway and ion transportation, and induced apoptosis and inflammation in the olfactory bulb. In conclusion, our results demonstrated that an environmentally realistic level of TCS/TCC would weaken the olfactory capacity of goldfish by constraining odorant recognition efficiency, disrupting olfactory signal generation and transduction, and disturbing olfactory information processing.
研究领域:
环境科学
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水生毒理学
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神经生物学
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鱼类生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）