Food Chemistry-X
·
2022
·
IF 6.443
Linlin Chen,Xiaofei Hu,Yunrui Xing,Yaning Sun,Mei Hu,Gaiping Zhang
In this study, a sensitive immunochromatographic assay (ICA) was developed for simultaneously detecting azaperone (AZN) and its metabolite azaperol (AZL) based on the high-affinity monoclonal antibody (mAb). Herein, the hapten AZL-SA was synthesized by succinic anhydride method, and then the conjugates AZL-SA-OVA and AZL-SA-KLH were prepared by EDC/NHS method. Subsequently, mAb was produced for targets monitoring through two detection modes. In direct ICA (using gold nanoparticles labeled specific antibodies), the visual LOD of AZN was 80 ng/g. For indirect ICA (using gold nanoparticles labeled anti-species antibodies), the visual and instrumental LODs of AZN were 8 and 0.14 ng/g, and AZL were 8 and 0.12 ng/g, respectively. The results indicated that the visual detection limit (LOD) of indirect format was tenfold lower than that of direct format. The established analytical method obtains the results within 15 min and provides a sensitive and simple tool for on-site detection of AZN and AZL.
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2023
·
IF 3.4
Huanhuan Yang,Cong Liu,Chuanhong Zhu,Yan Zheng,Juan Li,Qianyun Zhu,Hao Wang,Xiangming Fang,Quan Liu,Man Liang,Zilong Liu
Determination of antipsychotics from biological samples is meaningful in the field of clinical and forensic medicine. Compared to blood, keratinized matrices such as hair and nails have attracted increasing attention in drug analysis for wider detection window. Nevertheless, the distribution and stability of antipsychotics in keratinized matrices are not clarified yet. Therefore, we developed a LC-MS/MS based method for simultaneous determination of 10 antipsychotics from blood, hair and nails, with high recovery (78.1–107.4%, 71.3–93.5% and 75.2–90.5%), low LOD (5–10 pg/mL, 5–10 pg/mg and 5 pg/mg) and high accuracy (96.0%−101.6%, 97.5%−102.5% and 97.6–101.7%). The method was applied to sets of blood, hair, and nail samples of 54 patients who received long-term therapy, and significant correlations between drug concentrations in blood and hair was found, while the correlation between nails and other matrices varied depending on the drug. Except for olanzapine , the concentrations of antipsychotics in segmental hair samples were consistent with drug exposure. Besides, the hair and nail samples of suspects in two forensic cases were analyzed and provided supporting evidence for the suspects’ psychiatric state. Our research offered a deeper understanding of keratinized matrix as stable and retrospective bio-samples for antipsychotics detection in forensic practice.
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TALANTA
·
2022
·
IF 6.556
Mingqian Song,Changxin Li,Shijia Wu,Nuo Duan
Chlorpromazine is a phenothiazine representative drug that can be used to anesthetize and calm animals. However, chlorpromazine excess may lead to residual persistence in edible tissues, which is potentially harmful to human health and animal production. In this work, high affinity and specificity aptamers against chlorpromazine were screened out based on Capture-SELEX. After ten rounds of screening, the candidate aptamers were obtained. The optimal aptamer of CHL-3 was obtained by isothermal titration calorimetry (ITC) and SYBR Green I (SGI) fluorescence-competition methods. The Kd value of CHL-3 was 69.8 ± 9.81 nM. Subsequently, the Uio-66-NH 2 material was prepared, filled with rhodamine 6G (Rho 6G) dye into the pore, and sealed with CHL-3, and fluorescence probes were obtained. The ratiometric fluorescence detection method was established to detect the concentration of chlorpromazine. A linear relationship was obtained in a range of 1–100 nM, with a lower detection limit of 0.67 nM. Meanwhile, a good recovery was shown in spiked food samples, such as eggs and milk. These results indicate that the constructed ratiometric fluorescence strategy can be successfully applied in food detection.
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NEUROSCIENCE LETTERS
·
2022
·
IF 3.197
Tianyi Yang,Guang Yue,Yu Ge,Yue Zhang,Peng Xu,Youmei Wang,Bo Li,Bin Di
Nitrous oxide (N 2 O) has a long history of abuse, but its abuse mechanism has not been clear yet. This research aimed at the possibility of mesolimbic dopaminergic system (MLDS) involved in the rewarding effect of N 2 O. In this work, the rewarding behavior of N 2 O in mice was evaluated using a typical gas-administered conditioned place preference (CPP) procedure. SCH 23390, a Dopamine D1 receptor (D1R) antagonist, and Haloperidol, a Dopamine D2 receptor (D2R) antagonist were administered during CPP to evaluate the role of dopamine receptors in the N 2 O-induced CPP. The accompanying changes in phosphorylation of extracellular signal-regulated kinase (ERK) in MLDS related brain regions, including the ventral tegmental area (VTA), caudate putamen (CPu), prefrontal cortex (PFC), and nucleus accumbens (NAc) were measured to assess the neural plasticity changes in the CPP mice by Western blot analysis. Results revealed that 60% N 2 O induced CPP in the gas-administered mice and promoted the ERK phosphorylation (p-ERK) in the NAc and CPu during the test session of the CPP test. Pretreatment of SCH 23390 (0.5 mg/kg) inhibited the acquisition of N 2 O-induced CPP and the enhanced p-ERK in NAc. It suggested that Dopamine D1 receptor may play an important role in the acquisition of N 2 O-induced CPP and the accompanied ERK activation in the NAc, which provide insight into the molecular mechanism in the rewarding properties of nitrous oxide.
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TALANTA
·
2022
·
IF 6.556
Qianqian Shang,Huajing Liu,Hang Mei,Chuixiu Huang,Xiantao Shen
Coextraction of different groups of analytes is vital for saving sample volumes and simplifying analytical procedures in bioanalysis . Conventionally, coextraction was achieved by using multi-extraction systems with different supported liquid membranes (SLMs). However, the different membrane solvents tended to diffuse into the aqueous solutions and the other SLM to reach distribution equilibrium during extraction process, causing the system instability. In this work, a stable multi-extraction system (integration of liquid-phase microextraction and electromembrane extraction, LPME/EME) based on the identical supported semi-liquid membrane (SsLM) was developed. Principally, the state of distribution equilibrium of the membrane solvent (polypropylene glycol with molecular weight 4000) in SsLM could be reached at the beginning of extraction, which enhanced the coextraction stability. With this multi-extraction system, acidic and basic analytes were simultaneously extracted from practical biological samples. The extraction recoveries of the six model drugs in undiluted urine samples were over 70%. Followed by LC-MS/MS, the limits of quantification (LOQs) were in the range of 5–10 ng mL −1 . The multi-extraction system using the identical SsLM in this study shows promising potential in construction of other stable multi-extraction systems (e.g., LPME/LPME and EME/EME) in the future, which will greatly benefit the group separation of analytes in complicated biological samples.
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FOOD CHEMISTRY
·
2022
·
IF 9.231
Linlin Chen,Xiaofei Hu,Yaning Sun,Yunrui Xing,Gaiping Zhang
In this study, a novel hapten was designed and successfully synthesized to prepared the artificial immunogen against xylazine (XYL), then the monoclonal antibody 1H2-2C6 was obtained and applied to a lateral flow immunoassay (LFI) strip for rapid detection of XYL in milk. The visual detection limit (cutoff value) of the LFI strip was 5 ng/mL, the linear regression formula was Y = −0.521lg(X) + 0.382 ( R 2 = 0.995, n = 6), the linear detection range was 0.16–2.23 ng/mL and the detection limit (LOD) was 0.1 ng/mL. The recovery rates were in the range from 88.95% to 91.45%, the highest coefficient of variation was 8.89% (n = 3). The LFI strip was also validated by LC-MS/MS, as revealed, there were no significance differences between the two methods (the confidence interval was 95%). In brief, the LFI strip provides a reliable, sensitive and portable testing tool for screening of XYL on-site.
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PHARMACOLOGICAL RESEARCH
·
2021
·
IF 7.658
Yujiao Yang,Junke Song,NanNan Liu,Guangyi Wei,Shan Liu,Sen Zhang,Nan Jiang,Haiguang Yang,Guanhua Du
Chronic cerebral ischemia (CCI) refers to long-term hypoperfusion of cerebral blood flow with the main clinical manifestations of progressive cognitive impairment . The pathological mechanism of CCI is complex, and there is a lack of effective treatments. Salvianolic acid A (SalA) is a neuroprotective extract of Salvia miltiorrhiza with the effects of anti-inflammation and anti-apoptosis. In this study, the effect of SalA on cognitive function and Drd2/Cryab/NF-κB signaling pathway in rats with CCI was investigated. Morris water maze and open field test were used to observe the effects of SalA on the cognitive function of CCI rats. The pathological changes in the brain were observed by HE, Nissl, and LFB staining. TUNEL staining, enzyme-linked immunosorbent assay, and western blot analysis were used to detect the inflammatory and apoptosis in the cortex and hippocampus. The expression of Drd2/Cryab/NF-κB pathway-related molecules and Drd2 localization were detected by western blotting and dual immunofluorescence, respectively. SH-SY5Y cells were exposed to chronic hypoglycemic and hypoxic injury in vitro, and Drd2 inhibitor haloperidol was used to verify the involved pathway. The results showed that SalA could improve the cognitive function of CCI rats, reduce pathological damage of cortex and hippocampus, inhibit neuroinflammation and apoptosis, and suppress the activation of NF-κB by regulating Drd2/Cryab pathway. And SalA inhibited NF-κB activation and nuclear translocation in SH-SY5Y cells by upregulating Drd2/Cryab pathway, which was reversed by haloperidol interference. In conclusion, SalA could relieve CCI-induced cognitive impairment in rats, at least partly through the Drd2/Cryab/NF-κB pathway.
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ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Ruiqin Zhu,Hang Mei,Huajing Liu,Changbao Hong,Xiantao Shen,Chuixiu Huang
Practical biological and environmental samples always contain both acidic and basic substances, and the samples are always precious. Thus, separation of analytes with different nature from the same sample was of great significance. Successive liquid phase microextraction (sLPME) of acidic and basic analytes under optimal extraction conditions was therefore proposed for the first time. The concept of sLPME was proved by using three acidic analytes (naproxen, flurbiprofen and diclofenac) and three basic analytes (haloperidol, fluoxetine and sertraline) as model analytes, and using polypropylene glycol with an average molecular weight of 4000 (PPG4000) as SLM . The recoveries of all target analytes by sLPME were similar to that by individual LPME due to good affinity of PPG4000 to both acidic and basic analytes. Under optimal extraction conditions, the recoveries for all analytes by sLPME from urine samples were in the range of 62%–95%. Moreover, combined with LC-MS/MS, such sLPME approach was also evaluated with urine samples. The matrix effect of sLPME-LC-MS/MS at different levels for all analytes ranged from −14.1%–13.2%. The linear ranges with R 2 > 0.996 were 5–1000 ng mL −1 for basic analytes, and 20–1000 ng mL −1 for acidic analytes except diclofenac (1–1000 ng mL −1 ). The repeatability and accuracy at four levels were in the range of 3%–10% and 86%–120%, respectively. The limit of detection (LOD, S/N = 3) and limit of quantification (LOQ, S/N = 10) were found to be 0.07–0.49 ng mL −1 and 0.25–1.63 ng mL −1 , respectively. Finally, the strategy for constructing a sLPME system was further confirmed with urine, plasma and saliva using another two versatile SLM solvents possessing high affinity to both acidic and basic analytes. Successive LPME enabled separation of acidic and basic analytes from the same sample under optimum extraction conditions for all target analytes. Thus, we believe that the sLPME system will become a potent platform for forensic toxicology analysis, food science, environmental analysis and epidemiology study.
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高影响因子
GREEN CHEMISTRY
·
2021
·Vol. 23
·期 4
·1782-1793
·
IF 10.182
Hang Mei,Huajing Liu,Qianqian Shang,Ying Dong,Stig Pedersen-Bjergaard,Chuixiu Huang,Xiantao Shen
In this work, a green, stable and versatile organic-solvent-free electromembrane extraction (EME) system based on semi-interpenetrating polymer networks (semi-IPN) is proposed for the extraction and enrichment of both basic and acidic drugs from human urine. Because of its non-toxicity and high affinity in target separation, semi-solid polypropylene glycol (PPG) was selected as a component for the synthesis of semi-IPN networks via an ultrasonic-assisted blending (UAB) method. Using the homogeneous semi-IPN membrane as the supported liquid membrane (SLM), the organic-solvent-free EME system was obtained. Under the optimized conditions, the organic-solvent-free EME system showed high recovery efficiency for both basic analytes (70–97%) and acidic analytes (86–100%). Moreover, the organic-solvent-free EME set-up could be reused in eight successive extraction batches without an obvious decrease in extraction efficiency. In addition, the EME system could be conveniently stored at ambient temperature for a long time, which will greatly promote the commercialization of EME. We believe this study provides new insights and attempts in the development of green sample preparation approaches.
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JOURNAL OF CHROMATOGRAPHY A
·
2020
·
IF 4.049
Xiao Yu,Xinlin Li,Sunzhao You,Yuhang Shi,Ruiqin Zhu,Ying Dong,Chuixiu Huang
Separation of antipsychotic drugs from whole blood and urine is of great importance for clinic and forensic laboratories. In this work, chlorprothixene, haloperidol and risperidone representing the first and second generations of antipsychotic drugs were studied. Among them, chlorprothixene and risperidone were investigated for the first time by electromembrane extraction (EME). After the screening, 2-nitrophenyl octyl ether (NPOE) was used as the supported liquid membrane (SLM). The EME performance for spiked water (pH 2), whole blood and urine was tested and optimized individually. Using NPOE and 60 V, efficient EME was achieved from urine and whole blood with trifluoroacetic acid as the acceptor solution. The equilibrium time required for EME was dependent on the sample matrices. The steady-state of EME was reached in 30 min and 20 min for whole blood and urine, respectively. At steady-state, the EME recoveries of the targets from different sample matrices were satisfactory, and were in the range of 74%-100%. The proposed EME approach combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was evaluated using whole blood and urine. The obtained linearity was 1-200 ng mL −1 , and the coefficient of determination (R 2 ) was ≥ 0.9853 for haloperidol and ≥ 0.9936 for chlorprothixene and risperidone. The limit of detection (LOD) and accuracy for all the targets ranged from 0.2-0.6 ng mL −1 and 102%-110%, respectively, and the repeatability at low (1 ng mL −1 ), medium (10 ng mL −1 ) and high (200 ng mL −1 ) concentration was ≤ 12% (RSD). Finally, the validated approach was successfully used to determine chlorprothixene, risperidone and haloperidol in whole blood and urine from rats, which were treated with chlorprothixene, risperidone and haloperidol at low therapeutic dose, respectively.
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ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Libin Wan,Bin Lin,Ruiqin Zhu,Chuixiu Huang,Stig Pedersen-Bjergaard,Xiantao Shen
Isolation of substances by liquid-phase microextraction (LPME) or electromembrane extraction (EME) is becoming more and more important in analytical chemistry. However, the understanding of the mass transfer in LPME and EME is limited, especially for highly concentrated samples. In this work, the mass transfer in LPME and EME from aqueous samples (0.5–200 mg L–1) was studied in terms of recovery, equilibrium time, flux, and mass transfer capacity. In both EME and LPME, high recoveries were achieved at low analyte concentration, and the recoveries decreased at high analyte concentration. For EME, the loss in recovery was partly compensated by increasing the extraction voltage (from 50 to 200 V), while the LPME recovery at high analyte concentration was improved by increasing the extraction time (from 30 to 180 min). EME was superior in terms of equilibrium time and flux, while LPME provided much higher mass transfer capacity especially for highly concentrated samples. Moreover, the recovery was much more sensitive to high analyte concentrations in EME than in LPME, and the EME recovery decreased significantly above 50 mg L–1, indicating that LPME could be used to isolate analytes in a wider concentration range than EME. We believe that this fundamental study will be of great importance for the selection of a suitable membrane-based microextraction technique.
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