JOURNAL OF HAZARDOUS MATERIALS
·
2026
·
IF 10.6
Ziyan Cheng,Xinru Wang,Zhimin Yang,Jingtong Yu,Nan Zhao,Chenyang Ji,Quan Zhang,Meirong Zhao
Neonicotinoid insecticides (NNIs) are ubiquitously detected in the environment, yet their comparative neurotoxicity and mechanisms remain insufficiently characterized. This study systematically evaluated the neurotoxic potential of eight common NNIs (imidacloprid, acetamiprid, thiacloprid, thiamethoxam, clothianidin, flonicamid, sulfoxaflor, and imidaclothiz) using human SH-SY5Y cells. Results demonstrated that all tested NNIs significantly reduced cell viability. Mechanistically, NNI exposure triggered excessive reactive oxygen species (ROS) production, inhibited superoxide dismutase (SOD) activity, and elevated malondialdehyde (MDA) levels, indicating severe oxidative stress. Notably, Exposure to NNIs caused a dissipation of mitochondrial membrane potential (MMP) and profoundly disrupted mitochondrial bioenergetics. Analysis of energy metabolism revealed that NNIs suppressed both the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR), suggesting an impairment of oxidative phosphorylation and glycolysis. These findings suggested that NNIs induced neurotoxicity through coordinated oxidative stress and mitochondrial dysfunction, resulting in global bioenergetic suppression characterized by concurrent impairment of oxidative phosphorylation and glycolysis. This study provides critical toxicological evidence for the environmental risk assessment of NNIs and underscores their potential impact on human neurological health.
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高影响因子
Analytical Methods
·
2023
·Vol. 15
·期 19
·2376-2381
·
IF 3.1
Lei Jiang,Lin Yuan,Shan Gao,Yingying Xiang,Fei Song,Wensi Ma,Jing Wan,Xiuling Ji,Yujiao Tu
Pesticides are enormously important to industry requirements and in agriculture for improving yields. Parathion is widely used to control pests in vegetables, fruits, and flowers. However, excessive use of parathion poses a threat to food safety, the environment, and human health. Due to low cost, ease to use, and high selectivity and sensitivity, a fluorescent nanoprobe is a candidate for parathion detection. Blue fluorescent carbon dots were prepared by a hydrothermal method with rutinum and o-phenylenediamine as precursors. The Rut-CDs were purified by using dialysis, thin layer chromatography, and a chromatographic column. Excellent linear ranges of 0–7.5 μg L−1 and 12.5–62.5 μg L−1 parathion were obtained with a low detection limit of 0.11 ng mL−1. In addition, the quenching mechanism of parathion to the fluorescence of Rut-CDs was illuminated. Furthermore, the nanoprobe was efficiently applied to determine the parathion content in Chinese cabbage, cantaloupe, and cowpea samples. It shows great potential to detect parathion.
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高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Xinyue Huang,Lei Jiang,Lin Yuan,Wensi Ma,Xintao Cui,Jiaxuan Liu,Xiuling Ji,YujiaoTu
Pesticides are chemicals widely utilized in various industries, particularly agriculture, to manage pests and enhance crop yields. However, the excessive use of pesticides, such as phoxim, poses threats to the environment, food safety, and human health. Thus, it is crucial to use pesticides judiciously, limiting their application to what is necessary. Fluorescent probes have garnered attention for their cost-effectiveness, straightforward preparation, and high sensitivity, positioning them as promising candidates for detecting phoxim. In this context, orange ratiometric fluorescent carbon dots were synthesized using rhodamine B and NaOH as precursors through a hydrothermal method. The synthesized Rh B Na CDs were then purified via dialysis, thin-layer chromatography, and chromatographic columns to develop an accurate and precise ratiometric fluorescent probe. This nanoprobe demonstrated excellent linear ranges for detecting phoxim levels in various food samples. It exhibited a low detection limit of 4.486 nM for phoxim concentrations ranging from 0 to 25 μM and can accurately detect concentrations between 25 and 250 μM. Moreover, it proved sensitive in identifying phoxim content in samples of red grapes, green grapes, tomatoes, and pitayas.
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高影响因子
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