Toxics
·
2023
·Vol. 11
·期 4
·328
·
IF 4.6
Xiangqin Zheng,Yuexin Wei,Jiadong Chen,Xia Wang,Dinggang Li,Chengjun Yu,Yifan Hong,Lianju Shen,Chunlan Long,Guanghui Wei,Shengde Wu
Difenoconazole (DFZ) is a broad-spectrum triazole fungicide that is widely utilized in agriculture. Although DFZ has been demonstrated to induce reproductive toxicity in aquatic species, its toxic effects on the mammalian reproductive system have yet to be fully elucidated. In vivo, male mice were administered 0, 20 or 40 mg/kg/d of DFZ via oral gavage for 35 days. Consequently, DFZ significantly decreased testicular organ coefficient, sperm count and testosterone levels, augmented sperm malformation rates, and elicited histopathological alterations in testes. TUNEL assay showed increased apoptosis in testis. Western blotting results suggested abnormally high expression of the sperm meiosis-associated proteins STRA8 and SCP3. The concentrations of retinoic acid (RA), retinaldehyde (RE), and retinol (ROL) were increased in the testicular tissues of DFZ-treated groups. The mRNA expression level of genes implicated in RA synthesis significantly increased while genes involved in RA catabolism significantly decreased. In vitro, DFZ reduced cell viability and increased RA, RE, and ROL levels in GC-2 cells. Transcriptome analysis revealed a significant enrichment of numerous terms associated with the RA pathway and apoptosis. The qPCR experiment verified the transcriptome results. In conclusion, our results indicate that DFZ exposure can disrupt RA signaling pathway homeostasis, and induce testicular injury in mice testes.Keywords:difenoconazole;reproductive toxicity;testis;retinoic acid signaling;GC-2 cell;apoptosis
研究领域:
毒理学
·
生殖生物学
·
分子生物学
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DOI
PubMed
高影响因子
CHEMOSPHERE
·
2023
·
IF 8.8
Yajie Chen,Xingang Liu,Yanming Zhou,Lan Zhang,Liangang Mao,Lizhen Zhu,Yongquan Zheng
The aim of this study was to assess the bioavailability of epoxiconazole (EPO) and difenoconazole (DIF) in rice plants by evaluating their uptake, translocation, and accumulation. The results showed that the concentration of DIF in the roots was approximately three times higher than EPO, and both accumulated mainly in the roots. In addition, EPO continued to be transported from stems to leaves, causing a rise in its concentration in leaves. Contrastingly, only a minimal amount of DIF was transported to the leaves. This phenomenon is mainly governed by their differing octanol-water partition coefficient . The effects of dissolved organic carbon (DOC) on the accumulation of EPO and DIF in the roots were similar to those of the freely dissolved concentration measured by OECAMs . The concentrations of EPO and DIF in the roots and OECAMs consistently decreased with increasing DOC levels. Furthermore, a significant linear relationship was observed between the EPO and DIF concentrations in root and OECAMs. We also confirmed the accuracy and usefulness of the OECAMs method in predicting the bioavailability of EPO and DIF in rice roots. Therefore, OECAMs show good potential for use as a passive sampler to evaluate the bioavailability of EPO and DIF.
研究领域:
环境科学
·
植物生理学
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产品关联
DOI
PubMed
高影响因子
AUSTRALIAN JOURNAL OF CHEMISTRY
·
2021
·Vol. 74
·期 12
·838-846
·
IF 1.321
Xicheng Xie,Lingyun Li,Lumei Wang,Chaoqiang Pan,Dongwei Zhang,Guoqing Shen,Xicheng Xie,Lingyun Li,Lumei Wang,Chaoqiang Pan,Dongwei Zhang,Guoqing Shen
This paper illustrates the establishment of a colourimetric method for detection of the fungicide tebuconazole (TEB) in aqueous samples based on an unmodified TEB-specific aptamer and gold nanoparticles (AuNPs). In the absence of TEB, the AuNPs are coated with a TEB-specific aptamer and then stably dispersed a NaCl solution of high concentration, leading to a red solution and producing a maximum UV absorption peak at 520 nm. In the presence of TEB, due to the specific high affinity between TEB and the TEB-specific aptamer, the aptamer combines with TEB to form stable compounds, causing the AuNPs to be exposed in the solution and aggregate. The aggregated AuNPs turn the solution from red to blue, presenting a maximum UV absorption peak at 650 nm. Therefore, the concentration of TEB in the system can be quantitatively detected through the changes in absorbance. This TEB selective colourimetric biosensor detects TEB over a linear concentration range of 20 to 400 nM (R = 0.99385) and has a limit of detection (LOD) of 4.13 nM. The average recovery of TEB is 94.9–104.8 % in the application of actual water samples with the relative standard deviations (RSD) ranging from 1.01 to 5.34 %. With considerable sensitivity and selectivity, this aptasensor indicates great potential for TEB detection in aqueous samples.
研究领域:
分析化学
·
环境科学
·
纳米技术
·
生物传感器
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2015
·
IF 3.391
Enze Sheng,Haiyan Shi,Liangliang Zhou,Xiude Hua,Lu Feng,Tong Yu,Minghua Wang
Europium (Eu 3+ ) and samarium (Sm 3+ ) were used as fluorescent labels to develop a highly sensitive dual-labeled time-resolved fluoroimmunoassay (TRFIA) for detect clothianidin and diniconazole in food samples. Under the optimized assay conditions, 50% inhibition concentration (IC 50 ) and the limit of detection (LOD, IC 10 ) of clothianidin were 5.08 and 0.021 μg/L, and 13.14 and 0.029 μg/L for diniconazole. The cross-reactivities (CRs) were negligible except dinotefuran (9.4%) and uniconazole (4.28%). The recoveries of clothianidin and diniconazole ranged from 79.3% to 108.7% in food samples. The results of TRFIA for the authentic samples were validated by gas chromatography (GC) analyses, and a satisfactory correlations were obtained. These results indicated that the method was an alternative tool for simultaneous detection of clothianidin and diniconazole in food samples.
研究领域:
分析化学
·
食品安全
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DOI
高影响因子
Materials Chemistry Frontiers
·
2023
·Vol. 7
·期 5
·955-963
·
IF 7
Yang Liu,Liqiang Liu,Xinxin Xu,Chuanlai Xu,Liguang Xu
As a commonly used crop fungicide with proven adverse effects against various organisms, the excessive use of flutriafol (FTF) has resulted in accumulation of a large amount of residues in crops. In this study, a novel hapten was designed by introducing a long interval arm on its benzene ring allowing sufficient exposure of the FTF active site, for the production of a highly sensitive and specific monoclonal antibody with a half-inhibitory concentration of 1.77 ng mL−1. Furthermore, for the first time, a gold nanoparticle-based lateral flow immunoassay (LFIA) was developed for the quantitative detection of FTF in foods with a visual limit of detection in rice, tomatoes, and grapes of 0.5 mg kg−1, 0.2 mg kg−1 and 0.5 mg kg−1, respectively, and a cutoff value of 4 mg kg−1, and a calculated limit of detection of 0.045 mg kg−1, 0.035 mg kg−1 and 0.060 mg kg−1, respectively. The recoveries in spiked samples ranged from 90.2% to 110.1% and these results indicated that the gold nanoparticle-based LFIA developed in this study for the rapid detection of FTF in food is a reliable on-site detection method.
研究领域:
农业化学
·
食品科学
·
分析化学
·
环境科学
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产品关联
DOI
高影响因子
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
·
2023
·Vol. 116
·期 12
·1385-1393
·
IF 2.6
Meng Min,Zhai Zhaochi,Zhang Zhenxing,Kim Jeonghan,Zhu Yongzhe
Tebuconazole is the most widely used fungicide in agriculture. Due to its long half-life, tebuconazole residues can be found in the environment media such as in soil and water bodies. Here, the metabolic pathway of tebuconazole was studied in Cunninghamella elegans ( C. elegans ). Approximately 98% of tebuconazole was degraded within 7 days, accompanied by the accumulation of five metabolites. The structures of the metabolites were completely or tentatively identified by gas chromatography-mass spectrometry (GC–MS) and ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). To identify representative oxidative enzymes that may be involved in the metabolic process, treatment with piperonyl butoxide (PB) and methimazole (MZ) was performed. PB had a strong inhibitory effect on the metabolic reactions, while MZ had a weak inhibitory effect. The results suggest that cytochrome P450 (CYP) and flavin-dependent monooxygenase are involved in the metabolism of tebuconazole. Based on the results, we propose a metabolic pathway for the fungal metabolism of tebuconazole. Data are of interest to gain insight into the toxicological effects of tebuconazole and for tebuconazole bioremediation.
研究领域:
环境科学
·
微生物学
·
毒理学
·
生物修复
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENT INTERNATIONAL
·
2023
·
IF 11.8
Tingting Ku,Yutong Liu,Yuanyuan Xie,Jindong Hu,Yanwen Hou,Xin Tan,Xia Ning,Guangke Li,Nan Sang
Tebuconazole, one of the most widely used triazole fungicides, is reported to potentially pose a risk of inducing neurological disorders in human beings. Considering the increasing exposure, whether it could influence cognitive function remains to be elucidated. Herein, we used a mouse model to evaluate the potential cognitive risks and possible mechanisms from the continuous edible application of tebuconazole at low concentrations. Our study revealed that tebuconazole deteriorated spatial learning and memory and downregulated the expression of glutamate receptor subunits. Importantly, metagenomic analysis indicated that tebuconazole not only led to significant shifts in the composition and diversity of the gut microbiota but also changed intestinal homeostasis. Specifically, after exposure, tebuconazole circulated in the bloodstream and largely entered the gut–brain axis for disruption, including disturbing the Firmicutes/Bacteroidetes ratio, interrelated neurotransmitters and systemic immune factors. Moreover, pretreatment with probiotics improved immune factor expression and restored the deterioration of synaptic function and spatial learning and memory. The current study provides novel insights concerning perturbations of the gut microbiome and its functions as a potential new mechanism by which tebuconazole exposes cognitive function-related human health.
研究领域:
神经科学
·
微生物学
·
药理学
·
免疫学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Longhui Luo,Dongmei Chen,Xiufang Yan,Chao Kang,Wei Tian,Qian Liu,Xiaoying Yang,Tingting Chen,Youhua Long
Triazole pesticides are widely used to control pathogenic fungi in agricultural production, but the irrational use of these pesticides will have many negative effects on human health and ecological environment. In this study, we constructed a relatively complete visual monitoring system for triazole pesticides based on surface-enhanced Raman spectroscopy (SERS). Silver nanoparticles (Ag@ICNPs) modified with iodide and calcium ions showed stronger SERS enhanced ability and better reproducibility, and the characteristic peaks of eight different triazole pesticides were captured simultaneously. Principal component analysis (PCA) and support vector machine (SVM) were used to identify these pesticides. The developed method has strong quantitative analysis ability, excellent detection sensitivity and wide detection range, and has been successfully applied to the determination of pesticide residues in fruits and vegetables. In addition, dynamic SERS imaging technology combined with multivariate curve resolution-alternating least squares (MCR-ALS) to visually monitor the penetration behavior of triazole pesticides could remove the influence of interfering substances, thus obtaining an accurate distribution map of pesticides, which promoted the quantification of pesticide penetration process. The diffusion ability of different systemic pesticides into fruits and vegetables was revealed. Electrochemical (EC) assisted SERS of triazole pesticides showed that potential-induced molecular reorientation of pesticide molecules on the electrode surface was induced by the polarization of active substrate, which significantly improved the SERS signal of pesticides. Overall, this systematic pesticide monitoring study not only provides an effective innovative scheme for controlling the detection of hazardous substances in agricultural products, but also brings significant prospects for food safety guarantee.
研究领域:
食品安全
·
分析化学
·
农业科学
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产品关联
DOI
高影响因子
Food Chemistry-X
·
2025
·
IF 8.2
Yuxin Wang,Jin Liu,Suzhen Li,Jizhen Fu,Xiaowen Wang,Li Li,Xu Jing
The widespread use of triazole fungicides has raised concerns over residual. This study presents an automated, environmentally friendly, simple, and efficient method for detecting triazole fungicides in food samples, utilizing high-throughput liquid processing, dispersive liquid-liquid microextraction, and ultra-high-performance liquid chromatography-tandem mass spectrometry. The automated liquid handling workstation was employed to automate the whole sample pre-treatment process. Fatty acid, bio-based solvent, and saturated sodium chloride solution were used as the extractant, dispersant, and demulsifier, respectively. The whole operation process obviated the use of toxic reagents and eliminated the need for time-consuming centrifugation. The linear ranged from 0.01 to 1 μg L −1 with limits of detection and quantification of 0.003 μg L −1 and 0.01 μg L −1 , respectively. Ten triazole fungicides were successfully detected in food samples with recoveries of 70.1 %–105.7 %. The automation of dispersive liquid-liquid microextraction demonstrates huge potential for broad applicability within the field of food safety testing.
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Jin Liu,Yuanjun Nie,Yu Niu,Li Li,Xu Jing
A universal, green, and rapid lignin-based emulsive liquid-liquid microextraction (ELLME) method was established to detect nine triazole fungicides in water, juice, vinegar , and alcoholic beverages via UHPLC-MS/MS. By employing an environmentally friendly emulsifier (lignin), the proposed ELLME was compatible with more extractants, and not restricted to fatty acids. Due to the high amphiphilic properties and three-dimensional structure of lignin, the emulsion was quickly formed through several aspirate-dispense cycles of the green extractant (guaiacol) and lignin solution. And a micropipette was used for rapid microextraction . The limit of detection was 0.0002–0.0057 μg L −1 . The extraction recoveries and relative standard deviation were 81.7%–102.0% and 0.9%–7.1%, respectively. Finally, three green metric tools were used to verify the greenness of the whole procedure. The proposed lignin-based ELLME successfully emulsified green solvents , indicating that emerging solvents may be excellent alternatives as extractants in ELLME for pesticide residue analysis in food samples.
研究领域:
分析化学
·
环境科学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2021
·Vol. 29
·期 17
·25581-25590
·
IF 4.223
Li Haocong,Li Yong,Wang Wenfeng,Wan Qun,Yu Xiangyang,Sun Wenjing
Triazole pesticides are widely used for the control of pathogenic fungi in crops, which were frequently detected in edible parts. Its extensive use has caused many environmental pollution and food safety problems. In this study, the uptake, translocation, and subcellular distribution of three triazole pesticides (triadimefon, tebuconazole, and epoxiconazole) in rice were investigated. The results showed that the three triazole pesticides could be taken up by rice roots, but their distribution in plant tissues were different. The accumulation of the three pesticides in rice root followed the order of epoxiconazole (4.26 mg/kg, 24 h) > tebuconazole (2.63 mg/kg, 24 h) > triadimefon (1.37 mg/kg, 24 h), while a reversed order was observed in rice shoots, triadimefon (0.48 mg/kg, 24 h) > tebuconazole (0.40 mg/kg, 24 h) > epoxiconazole (0.21 mg/kg, 24 h). The translocation of triazole pesticides within rice tissues involved both symplast and apoplast pathways, with triadimefon preferentially through by the apoplast pathway and epoxiconazole through by the symplast pathway. The proportions of triadimefon, tebuconazole, and epoxiconazole in the symplast and apoplast of rice plants were 15–33%, 6–31%, 7–37%, and 67–85%, 69–94%, 63–93%, respectively. The subcellular distribution revealed that all pesticides have a higher proportion in cell walls than in cell organelles and soluble components. Epoxiconazole has the highest accumulated capacity in the cell wall (45–67%) and triadimefon was more concentrated in the soluble components (24–29%). However, there were no significant differences in the amount of three pesticides in cell organelles. The distribution of the three pesticides in aboveground and underground parts of rice plant, uptake and transportation in symplast and apoplast pathways, and distribution in the subcellular tissue are all related to their hydrophobicity.
研究领域:
农业科学
·
环境科学
·
植物生理学
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产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2022
·
IF 10.753
Jianan Liu,Jinjin Cheng,Chunli Zhou,Liya Ma,Xiaolong Chen,Yong Li,Xing Sun,Xiaolong Yan,Renhua Geng,Qun Wan,Xiangyang Yu
Food safety problems caused by pesticide residues have always been a concern for many people. In this study, we investigated the uptake, translocation and subcellular distribution of neonicotinoid insecticides, triazole fungicides, and sulfonylurea herbicides in rice plants ( Oryza sativa L.). The time-dependent uptake kinetics of the three categories of pesticides with different molecular structures fit a first-order one-compartment kinetic model. The neonicotinoids (log K ow −0.66–0.8) were mainly concentrated in the leaves, and the triazoles (log K ow 3.72–4.4) were mainly concentrated in the roots. Neonicotinoid pesticides in the roots were preferentially transported across the membrane through the symplastic pathway; triazole pesticides except for triadimefon and myclobutanil preferentially passed through the symplastic pathway; and sulfonylurea pesticides (log K ow 0.034–2.89) were first transported upward through the apoplastic pathway. In the roots, neonicotinoids, triazoles, and sulfonylurea herbicides were mainly concentrated in the soluble fractions, cell wall and apoplast fractions, respectively. In addition, there was a high positive correlation between the subcellular distribution of pesticides in the roots, stems and leaves. Molecular weight and log K ow jointly affected the enrichment of triazole pesticides in the roots, stems and leaves and the transfer from stems to leaves, while water solubility and log K ow commonly affected neonicotinoids. There was a correlation between pesticide absorption and the molecular structures of pesticides. To develop pesticides with strong uptake and transport capabilities, it is necessary to consider that the electronegativity of some atoms is stronger, the sum of the topological indices of heteroatoms can be large, and the van der Waals volume increases accordingly.
研究领域:
农业科学
·
植物生理学
·
环境毒理学
·
农药化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）