JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Tianyi Zhang,Changwei Gong,Jian Pu,Anchun Peng,Jizhi Yang,Xuegui Wang
Solenopsis invicta, an extremely destructive invasive species, has rapidly spread in China, with queens exhibiting chemical tolerance. In this study, bioassays were conducted on S. invicta colonies collected in Nanchong, revealing that the LC50 value of flonicamid for queens (3.91 mg/L) was significantly higher than that for workers (1.07 mg/L). Comparative analysis of transcriptomes of workers and queens treated with flonicamid revealed that differentially expressed genes (DEGs) were significantly enriched in the metabolism of xenobiotics by cytochrome P450 and drug metabolism by cytochrome P450 pathways. Based on the screening of transcriptome data, CYP6AQ83 might be involved in the detoxification metabolism of flonicamid in queens. After RNA interference, the sensitivity of queens to flonicamid was significantly increased by 30% in the treatment of the dsCYP6AQ83 group. Furthermore, heterologous overexpression of CYP6AQ83 in Drosophila melanogaster also significantly enhanced the tolerance against flonicamid. These results indicated that the overexpression of CYP6AQ83 in the queen enhances the tolerance against flonicamid.
研究领域:
昆虫学
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分子生物学
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害虫防治
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DOI
PubMed
高影响因子
Insects
·
2025
·Vol. 16
·期 3
·267
·
IF 2.9
Chunyan Yin,Ziyi Chen,Wei Chen,Zhenyu Wang
Simple SummaryManagingPlutella xylostellaandSpodoptera exiguahas traditionally relied on chemical insecticides, but resistance to these chemicals has become a major challenge in controlling these pests. Introducing new insecticides with a different mode of action is crucial for effective resistance management. Our study shows that fluxametamide exhibits strong insecticidal activity againstP. xylostellaandS. exigua. We assessed the susceptibility of field populations from key vegetable-growing regions in China and established a baseline of susceptibility to fluxametamide. The findings indicated thatP. xylostellaandS. exiguaare highly susceptible to this insecticide, and the baseline susceptibility data will serve as a reference for future resistance monitoring inP. xylostellaandS. exiguamanagement.Fluxametamide, an innovative isoxazoline insecticide, acts as an antagonist of γ-aminobutyric acid-gated chloride channels. Its distinct mode of action sets it apart, lacking known cross-resistance with current insecticides. This positions fluxametamide as a promising tool for addressing insecticide resistance in Lepidoptera, thysanoptera, coleoptera, and diptera pest insects. To develop and implement successful resistance management strategies, it is crucial to establish the baseline susceptibility to this insecticide before it is registered and widely used in China. In this study, we assessed the baseline susceptibility of two widespread lepidopteran pest species,Plutella xylostellaandSpodoptera exigua, to fluxametamide. The insecticide exhibited remarkably high efficacy against populations of the two lepidopteran species sampled in their primary distribution areas in China. ForP. xylostellaandS. exigua, the median lethal concentrations (LC50) ranged between 0.040 and 0.247 mg/L, and 0.211 and 0.761 mg/L, respectively. Among populations, there was a relative low variability in susceptibility to fluxametamide, showing a 6.18-fold difference forP. xylostellaand 3.61-fold forS. exigua. The suggested diagnostic concentrations forP. xylostellaandS. exiguawere 10 and 15 mg/L, respectively. Fluxametamide exhibited high toxicity to the selected resistant strains, which displayed strong resistance to abamectin, emamectin benzoate, and deltamethrin. No cross-resistance to fluxametamide was detected in the laboratory diamide-resistant strain. Our findings offer essential insights for crafting successful resistance management initiatives to maintain the effectiveness of fluxametamide against these significant pests.
研究领域:
昆虫学
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害虫管理
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农业科学
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DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2023
·
IF 4.7
Tian-yi Zhang,Chang-wei Gong,Jian Pu,An-chun Peng,Xu-yang Li,Yu-meng Wang,Xue-gui Wang
Solenopsis invicta is a main issue in southern China and is causing significant damage to the local ecological environment. The extensive use of insecticides has resulted in the development of tolerance in S. invicta . In our study, ten S. invicta colonies from Sichuan Province exhibited varying degrees of tolerance against flonicamid, with LC 50 values from 0.49 mg/L to 8.54 mg/L. The sensitivity of S. invicta to flonicamid significantly increased after treatment with the P450 enzyme inhibitor piperonyl butoxide (PBO). Additionally, the activity of P450 in S. invicta was significantly enhanced after being treated with flonicamid. Flonicamid induced the expression levels of CYP4aa1 , CYP9e2 , CYP4C1 , and CYP6A14 . The expression levels of these P450 genes were significantly higher in the tolerant colonies compared to the sensitive colonies, and the relative copy numbers of CYP6A14 in the tolerant colonies were 2.01–2.15 fold. RNAi feeding treatment effectively inhibited the expression of P450 genes, thereby reducing the tolerance of S. invicta against flonicamid. In addition, the overexpression of CYP6A14 in D. melanogaster resulted in reduced sensitivity to flonicamid. Our investigations revealed hydrophobic interactions between flonicamid and seven amino acid residues of CYP6A14, along with the formation of a hydrogen bond between Glu306 and flonicamid. Our findings suggest that flonicamid can effectively control S. invicta and P450 plays a pivotal role in the tolerance of S. invicta against flonicamid. The overexpression of CYP6A14 also increased tolerance to flonicamid.
研究领域:
昆虫学
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分子生物学
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毒理学
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PubMed
高影响因子
MOLECULES
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2019
·Vol. 24
·期 3
·562
·
IF 3.06
Qi-Bo Li,Min Liao,Qing Liu,Tong Feng,Zhi-Yuan Xu,Chang-Hui Rui,Shang-Zhong Liu
New 1,3,5-trimethylpyrazole-containing malonamide derivatives based on pyflubumide were designed, synthesized, and characterized using1H-NMR,13C-NMR, and high-resolution mass spectra (HRMS). The results of preliminary bioassays showed that the target compounds possessed good activities againstTetranychus cinnabarinus,Plutella xylostella, andAphis craccivora. Most of the target compounds exhibited moderate to good acaricidal activity againstTetranychus cinnabarinusat a concentration of 400 µg/mL, and some showed moderate activity at a concentration of 200 µg/mL; in particular, compounds8mand8pexhibited 70.0% mortality. In addition, some of the target compounds exhibited good insecticidal activities againstPlutella xylostellaat a concentration of 200 µg/mL, especially compounds8iand8o,which achieved 100.0% mortality at a concentration of 100 µg/mL. Interestingly, some of the target compounds exhibited potent anti-aphid activity againstAphis craccivoraat a concentration of 200 µg/mL; furthermore, compounds8pand8qdemonstrated 100.0% anti-aphid activity at a concentration of 50 µg/mL. The preliminary analyses of the structure–activity relationships (SAR) indicated that the acaricidal and insecticidal activities varied significantly depending on the type of substituent and substitution pattern, which provides guidance for the further investigation of such structural modifications.Keywords:malonamide;pyflubumide;synthesis;acaricidal and insecticidal activities
研究领域:
农药化学
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有机合成
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植物保护
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PubMed
高影响因子
CRYSTAL GROWTH & DESIGN
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2022
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IF 4.01
Mengnan Li,Jinling Wang,Xiaoyong Xu,Guobin Ren,Bin Zhu,Minghuang Hong,Zhong Li
Cycloxaprid (CYC) is a potentially commercially available pesticide with high activity, but its further development is limited due to the poor solubility and stability. In this paper, the co-crystallization strategy is applied to improve the physical and chemical properties of CYC for the first time. The cycloxaprid-succinimide (CYC–SM) co-crystal and the cycloxaprid-trifluoromethanesulfonamide (CYC–TS) co-crystal with a stoichiometric ratio of 1:1 between API and co-crystal former were successfully prepared through experimental screening and systemically characterized by powder X-ray diffraction, nuclear magnetic resonance, differential scanning calorimetry, thermogravimetry, Fourier transform infrared spectroscopy, and single-crystal X-ray diffraction techniques. The weak interaction of CYC–SM and CYC–TS co-crystals was quantified and analyzed by quantum chemistry calculations. Compared with CYC–TS, the molecular orbital and lattice energy calculation results of CYC–SM show lower chemical reactivity and better stability, which is consistent with the results of solid-state and solution stability tests. The partially weak interaction remained in the solution, and the weakly basic nature of SM and the relatively slow release of CYC from CYC–SM contributed to the promoted solution stability of CYC–SM. In addition, due to the introduction of SM molecules, the solubility of CYC–SM was significantly improved. The CYC–SM co-crystal could be a candidate solid-state form that provides an opportunity for the further development of CYC.
研究领域:
农药化学
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材料科学
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DOI
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2024
·
IF 10.8
Jia Zhang,Aihong Wu,Lingling Guo,Xiaoling Wu,Chuanlai Xu,Hua Kuang,Xinxin Xu
Flubendiamide (FLU), a widely used diamide insecticide, has been observed to potentiate adipogenesis in 3T3-L1 preadipocytes in vitro. Whether exposure to FLU disrupts hepatic lipid homeostasis in mammals and induces visceral obesity, however, remains unclear. The aim of this study was to assess the effects of FLU when administered orally to male C57BL/6J mice under normal diet (ND) and high-fat diet (HFD) conditions. FLU accumulated at higher levels in the tissues of the HFD group than those of the ND group, indicating that an HFD contributed to the accumulation of lipophilic pesticides in vivo. Notably, FLU (logP = 4.14) is highly lipophilic and easily accumulates in fat. Exposure to FLU had opposing effects on the lipid metabolism of the liver in the ND and HFD groups. Liver triacylglycerol levels in the ND group were reduced, while those in the HFD group were increased, resulting in more severe hepatic steatosis. More lipid accumulation was also observed in HepG2 cells exposed to FLU. Changes in hepatic lipid deposition in vivo occurred as the enhanced transcriptional regulation of the genes involved in lipid uptake, de novo lipogenesis, and fatty acid β-oxidation (FAO). Moreover, an excessive increase in FAO caused oxidative stress, which in turn exacerbated the inflammation of the liver. This study revealed the disruptive effect of FLU exposure on hepatic lipid homeostasis, which may facilitate the triggering of nonalcoholic fatty liver disease in HFD-fed mice.
研究领域:
毒理学
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代谢生物学
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肝脏疾病研究
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PubMed
高影响因子
Journal of Macromolecular Science Part A-Pure and Applied Chemistry
·
2019
·
IF 1.163
Kechao Liao,Pengfei Li,Guilan Chen,Xueqing Qiu
Our main goal was to evaluate release mechanism of microcapsules with different core status. First, flufiprole microcapsules were directly encapsulated with chitosan (CS) and sodium dodecyl sulfate (SDS) through layer-by-layer (LbL) self-assembly. The changes of process parameters on the microcapsules characteristics (zeta potential, and morphology) were investigated. Also the release trends of flufiprole were determined and fitted with mathematical models. It was revealed that releases of three types of microcapsules were all very similar, but microcapsule with solid particles and oil suspending agent reached their peak values at 36–42, 42–48 h respectively, microcapsule with solution droplets cannot reach a maximum value. In the stage of initial burst release, Higuchi model performed better with oil suspending agent and solid particles, which was in agreement with the Fick's law. Core status being solution, release curves of the pesticide corresponded to zero-order which was in agreement with dissolution mechanism. In the stage of slow release, the values of R2 for solution and solid particles were all above 0.98, betokened the release phenomenon were in line with the first-order. On the other hand, release of oil suspending agent conformed to Higuchi model.
研究领域:
农药制剂
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控释技术
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高分子科学
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胶体与界面化学
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环境科学
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DOI
高影响因子
ACS Applied Materials & Interfaces
·
2020
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IF 8.758
Xinyue Yuan,Wei Jiang,Jing Wang,Huilin Liu,Baoguo Sun
High-performance, multiporous imprinted microspheres were prepared from nitrogen-doped carbon dots (N-CDs) using a one-pot reverse microemulsion surface-imprinting method. Here, the N-CDs were exfoliated from a common layer covalent organic framework in a top-down preparation, and an ionic liquid was added to improve the sensitivity and the fluorescence stability. The multiporous imprinted microspheres were successfully applied to flonicamid optosensing in fruits and vegetables with simultaneous analysis of 96 samples by multifunctional enzyme labeling. The fluorescence sensing procedure was performed on recyclable multiporous imprinted microspheres coupling with the interface of N-CDs by taking advantage of the fluorescence-resonance charge-transfer strategy between the N-doped carbon dots and flonicamid molecules, quenching the fluorescence intensity. The multiporous imprinted microspheres exhibited purple fluorescence, which decreased sharply in intensity as the concentration of flonicamid increased. The fluorescence quenching correlation with the concentration of flonicamid showed good linearity in the range of 0.02–0.2 μg g–1 with a detection limit of 0.0059 μg g–1. This research not only enriches the foundational study of flonicamid residues but also greatly expands the potential applications of multiporous imprinted microspheres for analysis of pesticide residues in agricultural, food, and environmental monitoring.
研究领域:
材料科学
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分析化学
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环境科学
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农业科学
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DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2021
·
IF 6.165
Xiaofang Niu,Han Liu,Su Xue,Jinli Zhang,Wei Li
The solubility data of flonicamid (FA) in twelve mono-solvents (methanol (MA), ethanol (EA), n-propanol (NPA), isopropanol (IPA), n-butanol (NBA), isobutanol (IBA), ethyl acetate (EAC), butyl acetate (BAC), acetone (AT), acetonitrile (AN), tetrahydrofuran (THF), 1,4-dioxane (DX)) and a binary solvent of EA + EAC were experimentally measured by using a gravimetric method under atmospheric pressure. The tested temperature range for the studied solvents was from 283.15 K to 323.15 K except of 288.15–323.15 K for DX. Powder X-ray diffraction (PXRD) was applied to evaluate the crystal form of FA, and no solvate or new form formation were observed during the whole experiment. The experimental results show that the rising temperature leads to the increase of the FA solubility in all the studied mono and mixed solvents at the tested ranges. For mono-solvents, the solubility of FA at 293.15 K increases in the order: IBA (0.002641) < IPA (0.004243) < NPA (0.004361) < NBA (0.004750) < BAC (0.006844) < EA (0.006864) < EAC (0.01342) < MA (0.01394) < DX (0.01747) < AN (0.02455) < THF (0.03433) < AT (0.04645). For EA + EAC mixed solvents, the solubility of FA increases and then decreases with the rising mole fraction of EAC. That means addition of EAC to EA can alter the FA solubility. Density functional theory (DFT) calculations were performed to analyze the interactive energy between FA and the solvent molecules. Furthermore, the Apelblat equation, van’t Hoff equation, λh equation are applied to correlate the experimental solubility data of FA in mono and mixed solvent systems, while NRTL model and Wilson model are selected for mono-solvents, CNIBS/R-K model and Jouyban-Acree model for mixed solvents. Correlated results demonstrate that the Apelblat model provides the best regression. In addition, the dissolution thermodynamic properties of FA in chosen solvents are analyzed by the van’t Hoff equation. It suggests that the dissolution processes of FA are non-spontaneous, endothermic and entropy increase in these studied systems.
研究领域:
化学
·
物理化学
·
热力学
·
材料科学
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DOI
高影响因子
危险品化学品经营许可证（带存储）