FOOD CHEMISTRY
·
2022
·
IF 9.231
Wei Jia,Xin Wang,Lin Shi
Endogenous hydrocortisone causes detrimental effects on public health and domestic animal products, but the potential mechanisms remain elusive. Hydrocortisone was detected from seventy-two Guanzhong-Black pigs in three replicates (216 samples) (0.00 ± 46.38 μg kg −1 ), indicating the existence of endogenous hydrocortisone. Herein, we investigated the effects of hydrocortisone on the metabolic signatures in pork via integrative metabolomics and proteomics by UHPLC-Q-Orbitrap HRMS. Animal-derived foods under hydrocortisone-bioaccumulation cause metabolic perturbation by regulating glutamine synthetase expression at the transcriptional level contributed to hydrocortisone-induced toxicity and accelerating the down-regulation of essential amino acids ( l -Histidine 10.74–6.48 mg kg −1 , l -Phenylalanine 3.70–1.57 mg kg −1 ), purine nucleotides (GMP 40.29–5.00 μg kg −1 , etc) that provide nutritional value in a positive feedback loop. Protein-metabolite interactions suggesting that hydrocortisone enhanced nitric oxide synthase and GMP reductase expression (LOQ 3.24–63.39 μg kg −1 ), and affected meat flavor perception, eventually lead to the decline of nutritional value and flesh quality of pork.
研究领域:
动物学
·
食品科学
·
毒理学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Si-xuan Zhou,Xiao-tong Lin,Jie Wang,Hai-xiang Wang,Gui-tang Chen
In this work, magnetic molecularly imprinted polymers (MMIPs) were prepared by a surface imprinting method using Fe 3 O 4 nanoparticles as a support. The products were characterized by FT-IR spectroscopy, VSM, TGA, SEM, and TEM. Combined with HPLC, hydrocortisone in milk powder and milk were separated and purified, and their contents were monitored. The results showed that MMIPs with a particle size of approximately 1000 nm were successfully prepared. The adsorption mechanism of MMIPs was confirmed by kinetic adsorption and thermodynamic adsorption experiments; the maximum adsorption capacity was found to be 17.2 mg g −1 , and adsorption equilibrium could be reached within 40 min. In the actual sample application, the recovery rates of milk powder and milk were 93.88–99.15% and 95.80–98.10%, respectively. These results showed that MMIPs had good performance in selectively identifying hydrocortisone and were suitable for determining hydrocortisone in milk products.
研究领域:
分析化学
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食品科学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY
·
2017
·
IF 2.295
Kai Dong,Hefeng Zhang,Yan Yan,Jinyao Sun,Yalin Dong,Ke Wang,Lu Zhang,Xianpeng Shi,Jianfeng Xing
Context: Extensive or long-time use of corticosteroids often causes many toxic side-effects. The ion exchange resins and the coating material, Eudragit, can be used in combination to form a new oral delivery system to deliver corticosteroids.Objectives: The resin microcapsule (DRM) composed by Amberlite 717 and Eudragit S100 was used to target hydrocortisone (HC) to the colon in order to improve its treatment effect on ulcerative colitis (UC) and reduce its toxic side-effects.Methods: Hydrocortisone sodium succinate (HSS) was sequentially encapsulated in Amberlite 717 and Eudragit S100 to prepare the HSS-loaded resin microcapsule (HSS-DRM). The scanning electron microscopy (SEM) was employed to investigate the morphology and structure of HSS-DRM. The in vitro release and in vivo studies of pharmacokinetics and intestinal drug residues in rat were used to study the colon-targeting of HSS-DRM. The mouse induced by 2,4,6-trinitrobenzenesulfonic acid was used to study the treatment of HSS-DRM on experimental colitis.Results: SEM study showed good morphology and structure of HSS-DRM. In the in vitro release study, > 80% of HSS was released in the colon environment (pH 7.4). The in vivo studies showed good colon-targeting of HSS-DRM (Tmax = 0.97 h, Cmax = 118.28 µg/mL of HSS; Tmax = 2.16 h, Cmax = 64.47 µg/mL of HSS-DRM). Moreover, the HSS-DRM could reduce adverse reactions induced by HSS and had good therapeutic effects on the experimental colitis.Conclusions: The resin microcapsule system has good colon-targeting and can be used in the development of colon-targeting preparations.
研究领域:
药剂学
·
消化病学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2022
·
IF 3.571
Lu Wang,Xia Cui,Jiameng Xu,Gege Wang,Miao Guo,Liangwei Yu,Ke Yang,Zhimin Luo,Aiguo Zeng,Guoning Chen,Jia Zhang,Qiang Fu
Hydrocortisone (HC), as a common steroid hormone drug, is also one of the key intermediates involved in the synthesis of multiple steroid hormone drugs. Residual HC in pharmaceutical wastewater frequently pollutes environmental water as steroid hormone contaminant and possesses great threat to human health as well as sustainable development of the ecosystem. Herein, in order to develop a highly efficient adsorbent system for selective enrichment and detection of HC in pharmaceutical wastewater, a novel amino-functionalized aluminum-based metal organic frameworks (Al-MOFs@NH 2 ) mesoporous nanorod is fabricated, in which 2-aminoterephthalic acid plays a dual role as organic linker and functional modification unit. The resultant Al-MOFs@NH 2 not only exhibits stable mesoporous structure but also has large specific surface area (849.76 m 2 g −1 ) and plentiful binding sites, which significantly increases the adsorption capacity for HC. Under the promotion of hydrogen bonding and hydrophobic interaction together, Al-MOFs@NH 2 possesses high adsorption capacity (218.53 mg g −1 ) for HC, as well as shows satisfactory selectivity for HC and other steroid hormones. Moreover, a method using Al-MOFs@NH 2 as solid phase extraction adsorbents combined with high performance liquid chromatography (HPLC) has been developed to specifically enrich and detect trace amount of HC in pharmaceutical wastewater. The developed method has a low limit of detection (LOD) (0.5 × 10 −3 μg mL −1 ) and shows satisfactory recoveries for HC (75.9%−102.5%) with an acceptable relative standard deviation (RSD). These results demonstrate that the facile one-step preparation and excellent adsorption capacity makes Al-MOFs@NH 2 attractive to capture and remove environmental steroid hormone pollutants. More importantly, the method proposed in this work is expected to provide a prospective solution for analysis of strong bioactive contaminants in pharmaceutical wastewater.
研究领域:
环境科学
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药物化学
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分析化学
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产品关联
DOI
PubMed
高影响因子
Biomed Research International
·
2021
·
IF 3.411
Si Wen,Mengke Sun,Li Ye,Bin Yang,Lizhi Hu,Mao-Qiang Man
The benefits of emollients for eczematous dermatitis and psoriasis have been thought to be due to the improvements in epidermal function, including epidermal permeability barrier, stratum corneum hydration, and stratum corneum pH. We determined here whether emollient can direct inhibit cutaneous inflammation. Ear inflammation was induced by topical application of either 12-O-tetradecanoylphorbol-13-acetate (TPA) or 1-fluoro-2,4-dinitrofluorobenzene (DNFB). Either 1% hydrocortisone cream or the novel emollient was applied to the right ear of the mice 45 min and 2 hours after TPA or DNFB application. The untreated left ear served as untreated controls. Both ear weight and ear thickness were measured 24 hours after TPA and DNFB application. Topical applications of either hydrocortisone cream or emollient significantly decreased both ear thickness and ear weight in comparison to untreated controls. In DNFB model, hydrocortisone significantly lowered expression levels of mRNA for IL-1α, IL-1β, and TNFα, while the emollient markedly decreased expression levels of IL-1α and TNFα mRNA. In TPA model, both hydrocortisone and emollient significantly decreased expression levels of IL-1α, IL-1β, IL-6, and TNFα mRNA. In parallel, inflammatory infiltration was also reduced by topical applications of either hydrocortisone or emollient. These results demonstrate that this novel emollient can directly inhibit cutaneous inflammation in murine models of both acute irritant contact dermatitis and acute allergic contact dermatitis. However, whether this emollient could also alleviate eczematous dermatitis in humans remains to be explored.
研究领域:
皮肤病学
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免疫学
·
药理学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2021
·Vol. 168
·期 5
·057508
·
IF 4.316
Xia Cui,Jili Han,Guoning Chen,Lu Wang,Zhimin Luo,Chun Chang,Jia Zhang,Qiang Fu
The existence of hydrocortisone in water resources is a serious threat to human health. However, the rapid and sensitive detection methods for hydrocortisone in wastewater are still lacking. Meanwhile, due to the weak redox activity of hydrocortisone, electrochemical technique cannot be used to detect hydrocortisone directly. Therefore, molecularly imprinted technique combined with prussian blue nanoparticles was synthesized for detecting HC indirectly in this work. We fabricated a novel and highly sensitive molecularly imprinted sensor based on the polydopamine/electro-reduced graphene oxide, which was used for the specific recognition of hydrocortisone in water samples. The prussian blue nanoparticles were introduced as probes to reflect the concentration of the target. The morphology of the prepared sensors was characterized by the scanning electron microscopy. And the feature of the imprinted electrode was performed by the cyclic voltammetry and differential pulse voltammetry. Under the optimal experimental conditions, the relative current signal of prussian blue decreased linearly with the increase of hydrocortisone concentration with a limit of detection 0.006 nM. Moreover, the imprinted sensor had a much higher affinity towards hydrocortisone than other analogues. Then, this system was successfully applied to determine hydrocortisone in real wastewater samples with workable results.
研究领域:
环境科学
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分析化学
·
生物医学工程
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产品关联
DOI
高影响因子
Applied Materials Today
·
2020
·
IF 8.352
Liping Yuan,Meng Pan,Minyi Lei,Xingli Zhou,Danrong Hu,Qingya Liu,Yu Chen,Wei Li,Zhiyong Qian
Transdermal drug delivery is an attractive and potentially more effective method of localized and systemic treatment with minimal side effects and greater safety than current techniques. However, a limitation of drug delivery through the skin is the presence of the stratum corneum, which inhibits the development of clinical applications. Furthermore, in order to achieve better therapeutic outcomes, a number of traditional topically-applied preparations have high drug concentrations which can lead to skin irritation and formation of lesions. The use of safer and milder hydrocortisone (HC) cream for the treatment of moderate to severe atopic dermatitis (AD) is not considered due to its low potency and poor absorption through the skin, even at a dose of 1%. Therefore, in the present study, a composite of HC-loaded micelles and carbomer hydrogel was designed to enhance transdermal HC transport to improve topical therapy of AD. Franz diffusion cell experiments indicated that this composite significantly increased the skin permeation rate and cumulative quantity of HC transferred, which were greater than a 9-fold and 50-fold increase relative to HC cream, respectively. In addition, the composite system was demonstrated to rapidly and effectively ameliorate inflammatory symptoms on an AD-like mouse model and greatly reduce skin irritation and adverse systemic effects compared to HC cream. As described above, the novel HC formulation was a feasible strategy to provide safe and effective treatment in AD therapy.
研究领域:
药剂学
·
皮肤病学
·
药物递送系统
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Xiaoting Gao,Keliang Huang,Ai Zhang,Cihao Wang,Zhuyu Sun,Yanan Liu
Glucocorticoids (GCs) have drawn great concern due to their widespread contamination in the environment and application in treating patients with COVID-19. Due to the lack of data about GC removal using advanced treatment processes, a novel Paralleling and bubbling corona discharge reactor (PBCD) combined with iron-loaded activated-carbon fibre (Fe-ACF) was addressed in this study to degrade GCs represented by Hydrocortisone (HC) and Betamethasone (BT). The results showed that the PBCD-based system can degrade GCs effectively and can achieve effective sterilization. The removal rates of GCs were ranked as PBCD/Fe-ACF > PBCD/ACF > PBCD. The concentration of E. coli was reduced from 10 9 to 10 2 CFU/mL after 60 min of PBCD-based system treatment. The abundance of bacteria in actual Hospital wastewater (HWW) was significantly reduced. Plasma changed the physical and chemical properties of ACF and Fe-ACF by etching axial grooves and enhancing stretching vibrations of surface functional groups, thus promoting adsorption and catalytic degradation. For GC degradation, the functional reactive species were identified as •OH, 1 O 2 , and •O 2 radicals. Possible degradation pathways for HC and BT were proposed, which mainly included defluorination, keto acid decarboxylation, demethylation, intramolecular cyclization, cleavage and ester hydrolysis, indicating a reduction in GC toxicity. Since GCs are widely used in patients with COVID-19 and their wastewater needs to be sterilized simultaneously, the intensive and electrically driven PBCD-based system is promising in GC pollution control and sterilization in terminal water treatment facilities.
研究领域:
环境工程
·
水处理
·
等离子体化学
·
医疗废物管理
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产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Shengliang Zhang,Hongyun Lu,Hanghang Lou,Ying Shi,Donghong Liu,Qihe Chen
The large-scale production of cultured meat has been restricted by high cost of serum, batch difference and unclear composition, thus it is important to develop serum-free media for cell growth. Here we obtained a serum-free medium (SFM), which was suitable for ex vivo culturing of myoblasts from larimichthys crocea . It offered simple composition and high effectiveness that supporting myoblast attachment, expansion and differentiation. Our findings revealed that the optimal SFM consisted of DMEM/F12 base medium, 5 mg/mL bovine serum albumin (BSA), 20 mmol HEPES, 1 % penicillin/streptomycin mixture (PS) and the exogenous additive hydrocortisone. The living cells of SFM could occupy about 65 % of the serum-containing medium (SM). Additionally, differentiation assays showed that myoblasts cultured without serum still differentiated into more muscle tubes, with a higher fusion index than those cultured with serum. Finally, transcriptome sequencing was conducted to demonstrate that hydrocortisone might act as a ligand binding to G protein-coupled receptors, triggering the downstream PI3K/AKT signaling pathway and leading to cell proliferation. Our findings will provide a basis for large-scale cell expansion and cell culture meat production in the future.
研究领域:
细胞生物学
·
组织工程
·
生物技术
·
食品科学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2020
·
IF 4.845
Luyao Zheng,Zhiyuan Zhao,Ye Yang,Yaming Li,Chengxiao Wang
This study developed novel ionic liquids (ILs) based on amino acids. We first screened 15 methyl amino acid ester hydrochlorides ([AAC1]Cl) for their skin permeation enhancements using 5-Fluorouracil (5-Fu) and Hydrocortisone (HC) as model drugs. Glycine methyl ester hydrochloride ([GlyC1]Cl), L-proline methyl ester hydrochloride ([L-ProC1]Cl), and L-leucine methyl ester hydrochloride ([L-LeuC1]Cl) were selected, and their ester sites were modified with different carbon chains (C8 and C12). The resulting ILs showed improved permeation to the two drugs. TEWL and CLSM assays revealed the moderation effects of the modified ILs on skin barrier function, whereas L-proline dodecyl ester hydrochloride ([ProC12]Cl) and L-leucine dodecyl ester hydrochloride ([L-LeuC12]Cl) exhibited the strongest activities. Permeation mechanisms were further investigated by ATR-FTIR, solid-NMR, SEM, and TEM analyses. The results suggested that [L-ProC12]Cl and [L-LeuC12]Cl combined the advantages of amino acid esters and IL solvent and could interact with the intercellular lipid domain by the multi-functions of lipid fluidization and lipid extraction, which were observed in a dosage- and time-dependent manner. Additionally, pathological examination suggested that the amino acid ester-based ILs (AAE-ILs) had good biocompatibility . In conclusion, this study has generated novel chemical penetration enhancers (CPEs) based on AAE-ILs and may be potentially utilized in drug transdermal delivery systems (TDDSs).
研究领域:
药学
·
药物递送系统
·
药剂学
·
材料科学
·
生物物理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Rong Fang,Jiao Ma,Meiling Wang,Kunpeng Guo
Generally, it is essential to externally introduce redox probes for cortisol detection through molecularly imprinted electrochemical sensor. However, this approach complicates the detection process, hinders real-time monitoring and may cause significant interference during body fluid analysis. To address the issue, we innovatively proposed a self-signal sensor based on humic acid (HA)-mediated conductive redox nanoprobe. Amphiphilic HA can effectively coat multi-walled carbon nanotubes (MWCNT) through π-π interactions, providing exceptional dispersibility while retained conductivity. Additionally, due to its colloidal characteristics, the HA coated MWCNT exhibited remarkable adsorption capacity towards various types of redox molecules, particularly cationic redox species, thus acquiring a kind of conductive redox nanoprobe in a facile way. The nanoprobe could serve as a bottom layer to prepare an electrochemical sensor in combination with cortisol-imprinted polydopamine as the upper layer. Finally, a self-signal electrochemical sensor for cortisol was successfully fabricated, achieving a detection limit of 0.72 × 10 −9 M, a wide detection range from 1 nM to 1 mM and good recovery. Overall, we believe the HA-mediated strategy proposed in this study could inspire more innovation in the field of self-signal sensing.
研究领域:
电化学传感
·
生物传感器
·
环境化学
·
分析化学
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Xin Yu,Jingzhang Wang,Guanghao Huang,Lianqun Zhou,Zhenjie Zhang,Shuzhi Sam Ge,Qing Wang,Hui Kong
The demand for early detection of inflammation-related diseases has driven interest in non-invasive methods like sweat analysis for their simplicity and rapid response. This paper develops a non-invasive C-reactive protein (CRP) sensor based on a three-dimensional dendritic gold structure electrode, which features high sensitivity, broad detection range, and excellent specificity. By in-situ growing dendritic gold on the gold electrode surface, the specific surface area of the sensor is 2.41 times that of the gold electrode. This increases the binding sites for CRP-capturing antibody (cAb), enabling the sensor to achieve a sensitivity of 3.26 µA mL ng −1 , which is 3.33 times that of the gold electrode. A double-antibody sandwich assay is employed using thionine-labeled spherical gold nanoparticles-antibody (AuNPs-dAb) conjugates as detection probes, with bovine serum albumin (BSA) blocking to minimize interference. The approach enhances sensor specificity and accuracy while enabling low-concentration detection, achieving a linear detection range of 0.5–30 ng mL −1 . The sensor's detection results exhibited a correlation coefficient of 0.995 with hospital-standard instruments. Additionally, a sweat-processing device and a flexible printed circuit board (FPCB) are integrated. Data are transmitted to mobile terminals via low-power near field communication (NFC). This platform facilitates early inflammation detection in clinical and home settings.
研究领域:
生物传感器
·
可穿戴电子设备
·
即时检测
·
炎症生物标志物
·
传感用纳米材料
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）