ANALYTICAL CHEMISTRY
·
2016
·
IF 5.886
Fanpeng Kong,Yuehui Zhao,Ziye Liang,Xiaojun Liu,Xiaohong Pan,Dongrui Luan,Kehua Xu,Bo Tang
Hydrogen selenide (H2Se) is an important metabolite of dietary Se compounds and has been implicated in various pathological and physiological processes. The development of highly sensitive and selective methods for the sensing of H2Se is therefore very important. Herein, we developed a fluorescent probe (hemicyanine (Hcy)-H2Se) for detecting H2Se based on a new H2Se-specific receptor unit, 1,2-dithiane-4,5-diol. Hcy-H2Se showed high selectivity toward H2Se over thiols (RSH), hydrogen sulfide (H2S), and selenocysteine (Sec) and was further exploited for the fluorescence imaging of H2Se both in living cells and in vivo. Furthermore, with the aid of Hcy-H2Se, we demonstrated that H2Se can be generated and gradually accumulated in HepG2 cells under hypoxic conditions and in the solid tumor after treatment with Na2SeO3.
研究领域:
生物化学
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医学影像
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病理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 71
·40984-40988
·
IF 2.936
Xiaonan Gao,Wenfei Guo,Lihong Ge,Fanpeng Kong,Kehua Xu,Bo Tang
Sodium selenite (Na2SeO3), as an anti-tumor drug for inducing tumor cells' apoptosis, has been widely studied under normoxic conditions and has been shown to exhibit oxidative stress process-induced apoptosis. However, since the real tumor environment is hypoxic, the actual mechanism is still unclear. Hence, considering the main metabolite of Na2SeO3 in the metabolic process to be hydrogen selenide (H2Se) and also that it can be converted to superoxide anion (O2˙−) instantaneously in the presence of oxygen, a dual-ratiometric fluorescence imaging system for simultaneous monitoring of the changes of H2Se and O2˙− induced by Na2SeO3-guided tumor cell apoptosis under hypoxic conditions was constructed. Two molecular probes NIR-H2Se and dihydroethidium were used to detect H2Se and O2˙−, respectively, whereas Rhodamine 110 was used as the reference fluorophore. Notably, H2Se levels significantly increased under hypoxic conditions, but there was no change in the level of O2˙−, which is inconsistent with the results of the previous researches. Therefore, we hypothesize that the mechanism of Na2SeO3-induced apoptosis for tumor cells is caused by reductive stress; also, this method can be applied for the future study of other anti-cancer selenium compounds.
研究领域:
生物化学
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医学影像学
·
肿瘤学
✓ 已验证
DOI
高影响因子
Nano Today
·
2021
·
IF 20.722
Shaojun Peng,Hao Wang,Yongjie Xin,Wei Zhao,Meixiao Zhan,Jingchao Li,Rong Cai,Ligong Lu
Hydrogen selenide (H 2 Se) plays key roles in diverse physiological processes ; however, the noninvasive regulation of H 2 Se levels in living systems has been rarely achieved and the functional mechanism of H 2 Se in cancer cells remains unclear. Herein, biocompatible ferrous selenide (FeSe 2 ) nanoflowers with second near-infrared (NIR-Ⅱ) photoactivatable H 2 Se generation were developed for metastasis-inhibited cancer therapy. Upon NIR-II laser irradiation, FeSe 2 nanoflowers generated mild photothermal effect, leading to their decomposition and on-demand release of H 2 Se to induce cell death. Such a FeSe 2 nanoflower-mediated synergistic photothermal-H 2 Se gas therapy effectively suppressed the tumor growth in a subcutaneous breast tumor-bearing mouse model. Furthermore, the local generation of H 2 Se was proved to down-regulate the expression levels of metastasis-related proteins, contributing to the complete inhibition of liver and lung metastasis. Overall, we report a NIR-Ⅱ photoactivatable H 2 Se production strategy for metastasis-inhibiting cancer therapy, which may be mediated by autophagy, opening a new avenue for the biomedical application of H 2 Se in the future.
研究领域:
生物医学工程
·
癌症生物学
·
纳米技术
·
光疗
✓ 已验证
产品关联
DOI
高影响因子
BIOMATERIALS
·
2024
·
IF 12.8
Fan Yang,Rui Shu,Wenyu Dai,Bin Li,Chuang Liu,Hang Yang,Hannah M. Johnson,Sheng Yu,Ding Bai,Weizhong Yang,Yi Deng
Pathogenic infection leads to excessive senescent cell accumulation and stagnation of wound healing. To address these issues, we devise and develop a hydrogen selenide (H 2 Se)-evolving bio-heterojunction (bio-HJ) composed of graphene oxide (GO) and FeSe 2 to deracinate bacterial infection, suppress cellular senescence and remedy recalcitrant infected wounds. Excited by near-infrared (NIR) laser, the bio-HJ exerts desired photothermal and photodynamic effects, resulting in rapid disinfection. The crafted bio-HJ could also evolve gaseous H 2 Se to inhibit cellular senescence and dampen inflammation. Mechanism studies reveal the anti-senescence effects of H 2 Se-evolving bio-HJ are mediated by selenium pathway and glutathione peroxidase 1 (GPX1). More critically, in vivo experiments authenticate that the H 2 Se-evolving bio-HJ could inhibit cellular senescence and potentiate wound regeneration in rats. As envisioned, our work not only furnishes the novel gasotransmitter-delivering bio-HJ for chronic infected wounds, but also gets insight into the development of anti-senescence biomaterials.
研究领域:
生物材料
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伤口愈合
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生物医学工程
·
细胞生物学
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）