The efficacy of nanoparticle (NP)-based drug delivery technology is hampered by aberrant tumor stromal microenvironments (TSMs) that hinder NP transportation. Therefore, the promotion of NP permeation into deep tumor sites via the regulation of tumor microenvironments is of critical importance. Herein, we propose a potential solution using a dihydralazine (HDZ)-loaded nanoparticle drug delivery system containing a pH-responsive, cyclic RGD peptide-modified prodrug based on doxorubicin (cRGD-Dex-DOX). With a combined experimental and theoretical approach, we find that the designed NP system can recognize the acid tumor environments and precisely release the encapsulated HDZ into tumor tissues. HDZ can notably downregulate the expression levels of hypoxia-inducible factor 1α (HIF1α), α-smooth muscle actin, and fibronectin through the dilation of tumor blood vessels. These changes in the TSMs enhance the enrichment and penetration of NPs and also unexpectedly promote the infiltration of activated T cells into tumors, suggesting that such a system may offer an effective “multifunctional therapy” through both improving the chemotherapeutic effect and enhancing the immune response to tumors. In vivo experiments on 4T1 breast cancer bearing mice indeed validate that this therapy has the most outstanding antitumor effects over all the other tested control regimens, with the lowest side effects as well.
Two colorimetric and fluorescent probes based on ferrocene derivatives have been designed and synthesized. Among studied anions (F − , AcO − , H 2 PO 4 − , Cl − , Br − and I − ), probe 1 with p -nitrophenyl hydrazine structure has the strongest binding ability for F − , as well as probe 2 for H 2 PO 4 − . It had strong binding ability and was not affected by other anions. The determination limit of 1 toward F − was 5.0×10 −6 mol⋅L −1 which can be used for the trace detection of F − . The interacted process of host–guest accompanied by colorimetric changes and increment in fluorescent intensity of the probes. A 1 H-NMR titration experiment and theoretical investigation indicated that −NH in hydrazine participated in the interaction, and the hydrogen-bonding formed between the probes and anions. These results provided an essential clue to the developing the colorimetric probe based on hydrazine participation.
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