Chinese Journal of Natural Medicines
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2021
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IF 3
Di ZHANG,Bing YANG,Shi-Quan CHANG,Sheng-Suo MA,Jian-Xin SUN,Lin YI,Xing LI,Hui-Mei SHI,Bei JING,Ya-Chun ZHENG,Chun-Lan ZHANG,Feng-Guo CHEN,Guo-Ping ZHAO
This study was to investigate the protective effect of paeoniflorin (PF) on hydrogen peroxide-induced injury. Firstly, “SMILES” of PF was searched in Pubchem and further was used for reverse molecular docking in Swiss Target Prediction database to obtain potential targets. Injury-related molecules were obtained from GeenCards database, and the predicted targets of PF for injury treatment were selected by Wayne diagram. For mechanism analysis, the protein-protein interactions were constructed by String, and the KEGG analysis was conducted in Webgestalt. Then, cell viability and cytotoxicity assay were established by CCK8 assay. Also, the experimental cells were allocated to control, model (200 μmol·L −1 H 2 O 2 ), SB203580 10 μmol·L −1 (200 μmol·L −1 H 2 O 2 + SB203580 10 μmol·L −1 ), PF 50 μmol·L −1 (200 μmol·L −1 H 2 O 2 + PF 50 μmol·L −1 ), and PF 100 μmol·L −1 (200 μmol·L −1 H 2 O 2 + PF 100 μmol·L −1 ) groups. We measured the intracellular ROS, Hoechst 33258 staining, cell apoptosis, the levels of Bcl-xl, Bcl-2, Caspase-3, Cleaved-caspase3, Cleaved-caspase7, TRPA1, TRPV1, and the phosphorylation expression of p38MAPK. There are 96 potential targets that may be associated with PF for injury treatment. Then, we chose the “Inflammatory mediator regulation of TRP channels” pathway for the experimental verification from the first 10 KEGG pathway. In experimental verification, H 2 O 2 decreased the cell viability moderately ( P < 0.05), and 100 μmol·L −1 PF increased the cell viability significantly ( P < 0.05). Depending on the difference of intracellular ROS fluorescence intensity, PF inhibited H 2 O 2 -induced reactive oxygen species production in Schwann cells. In Hoechst 33258 staining, PF reversed the condensed chromatin and apoptotic nuclei following H 2 O 2 treatment. Moreover, Flow cytometry results showed that PF could substantially inhibit H 2 O 2 induced apoptosis ( P < 0.05). Pretreatment with PF obviously reduced the levels of Caspase3, Cleaved-caspase3, Cleaved-caspase7, TRPA1, TRPV1, and the phosphorylation expression of p38MAPK after H 2 O 2 treatment ( P < 0.05), increased the levels of Bcl-2, and Bcl-xl ( P < 0.05). PF inhibited Schwann cell injury and apoptosis induced by hydrogen peroxide, which mechanism was linked to the inhibition of phosphorylation of p38MAPK.
研究领域:
神经生物学
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药理学
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分子生物学
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DOI
PubMed
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 114
·94008-94015
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IF 3.84
Fen Guo,Ke Ye,Xiaomei Huang,Yinyi Gao,Kui Cheng,Guiling Wang,Dianxue Cao
A novel Pd/polyaniline/CFC electrode is prepared by electroless deposition of palladium (Pd) onto three-dimensional polyaniline networks. The polyaniline matrix on the carbon fiber cloth (CFC) in the reduction state is electro-synthesized by cyclic voltammetry and has a lower vertex potential of −0.4 V vs. Ag/AgCl. The particle size of the Pd coated on the polyaniline chains is gradiently distributed. The as-prepared Pd/polyaniline/CFC electrode is characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FITR) and X-ray diffraction (XRD). The hydrogen peroxide (H2O2) electro-reduction reaction in H2SO4 solutions on the Pd/polyaniline/CFC electrode is investigated using cyclic voltammetry (CV), linear sweep voltammetry (LSV), chronoamperometry (CA) and electrochemical impedance spectroscopy (EIS). The results reveal that the electrode exhibited high catalytic activity and excellent stability in the strong oxidizing solution of H2O2 and H2SO4. Polyaniline itself shows electro-catalytic activity towards H2O2 to some extent involving the chemical–electrochemical (C–E) coupling mechanism.
研究领域:
材料科学
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电化学
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DOI
高影响因子
INSTRUMENTATION SCIENCE & TECHNOLOGY
·
2018
·
IF 0.963
Fang Liu,Baihong An,Jingwei Zhang,Shuyan Niu,Jun Wan
A polythiophene–graphene oxide compound membrane and Prussian blue were deposited sequentially on the surface of a glassy carbon electrode by cyclic voltammetry. Due to its excellent electrocatalysis and its analogy with peroxidase enzymes, Prussian blue has been widely used in amperometric biosensors. The polythiophene–graphene oxide compound membrane exhibited good electroconductibility and a large specific surface area. The fabricated Prussian blue/polythiophene/graphene oxide/glassy carbon electrode was characterized by transmission electron microscopy, scanning electron microscopy, and cyclic voltammetry. Under the optimal experimental conditions, the detection of hydrogen peroxide was studied by its amperometric current–time curve. Due to the presence of polythiophene–graphene oxide compound membrane and Prussian blue, the hydrogen peroxide sensor shows a linear calibration range of 1.0 × 10−6–1.0 × 10−4 mol L−1, detection limits of 3.2 × 10−7 mol L−1 at a signal-to-noise ratio of 3, and recoveries from 95.0 to 105.0%. The results show that the modified glassy carbon electrode has potential practical application for the determination of hydrogen peroxide based on its sensitivity and long-term stability.
研究领域:
分析化学
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生物传感技术
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DOI
高影响因子
MICROCHIMICA ACTA
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2019
·Vol. 186
·期 5
·1-8
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IF 5.479
Zhao Qi,Zhou Chaohui,Yang Qingye,Chu Zhongyun,Jia Nengqin
In the Fenton reaction, ferrous ion acts as a catalyst and reacts with hydrogen peroxide (H 2 O 2 ) to produce hydroxy radicals (·OH) and hydroperoxy radicals (·OOH). Both have much stronger oxidization ability than H 2 O 2 . A fluorescent probe for H 2 O 2 is described here that was obtained by covalent conjugation of carbon quantum dots to gold nanoclusters (AuNCs). The conjugate, under 360 nm photoexcitation, displays dual (blue and red) emission, with peaks located at 450 and 640 nm. When introducing ·OH radicals via the Fenton reaction, the fluorescence intensities of both the CQDs and the AuNCs are decreased. The ratio of the fluorescence at the two peaks is related to the concentration of H 2 O 2 in the 1.25 nM to 10 μM concentration range, and the detection limit is 0.16 nM. The probe was applied to the determination of H 2 O 2 in milk and toothpaste and to cell imaging.
Schematic diagram of the FRET-based fluorescent probe and enhanced performance of hydrogen peroxide detection via Fenton reaction. The fluorescence intensity of CQD-AuNCs nanoaster was decreased as introducing H 2 O 2 to the probe, and can be applied to the determination of milk and toothpaste and cells imaging.
研究领域:
生物化学
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分析化学
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纳米技术
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2020
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IF 3.232
Yi-lin Chai,Zi-bo Gao,Zhuang Li,Lei-liang He,Fei Yu,Song-cheng Yu,Jia Wang,Yong-mei Tian,Li-e Liu,Yi-lin Wang,Yong-jun Wu
In this paper, a label-free fluorescence nanoprobe is constructed based on poly(thymine) single strand DNA-templated Copper nanocluster (denote as: T-CuNCs) for the detection of hydrogen peroxide. In the assay, the fluorescent T-CuNCs will generate though the reaction of Cu 2+ , poly(thymine) and sodium ascorbate . However, the hydroxyl radical ( . OH) will generated in the presence of H 2 O 2 , which is able to induced the oxidative lesions of poly(thymine) single chain DNA and lead to the poly(thymine) being splitted into shorter or single oligonucleotide fragments and lose the ability to template the fluorescent T-CuNCs again. Therefore, H 2 O 2 can be detected by monitoring the fluorescence strength change of T-CuNCs. The experimental results show that the fluorescence intensity change of T-CuNCs has fantastic linearity versus H 2 O 2 concentration in the range of 1–30 μM (R 2 = 0.9947) and 30–80 μM (R 2 = 0.9972) with the limit of detection (LOD) as low as 0.5 μM (S/N = 3). More important, the fluorescent nanoprobe was also successfully utilized on the detection of H 2 O 2 in serum samples. Therefore, a label-free, costless and effective fluorescence method has been established for the detection of H 2 O 2 , the intrinsic properties of the nanoprobe endow its more potential applications in chemical and biological study.
研究领域:
生物化学
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分析化学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 1
·1-10
·
IF 5.833
Zhu Junlun,Luo Guan,Xi Xiaoxue,Wang Yijia,Selvaraj Jonathan Nimal,Wen Wei,Zhang Xiuhua,Wang Shengfu
A Cu 2+ -modified carboxylated hollow carbon nanospheres (Cu 2+ -HCNSs-COOH) was designed with enhanced peroxidase-like activity for the detection of hydrogen peroxide (H 2 O 2 ) and degradation of methylene blue (MB). Hollow polymer nanospheres were fabricated from aniline, pyrrole, Triton-100, and ammonium persulfate via confined interfacial copolymerization reaction, which can be pyrolyzed to create HCNSs with the hollow gap diameter of about 20 nm under high temperature. Combining the synergistic effect of coordination and electrostatic interaction, Cu 2+ -HCNSs-COOH was constructed by anchoring Cu 2+ on the surface of HCNSs-COOH. Furthermore, Cu 2+ -HCNSs-COOH has higher affinity for 3,3′,5,5′-tetramethylbenzidine and H 2 O 2 of 0.20 mM and 0.88 mM, respectively. Based on the rapid response of Cu 2+ -HCNSs-COOH to H 2 O 2 , we constructed a colorimetric sensing platform by detecting the absorbance of the 3,3′,5,5′-tetramethylbenzidine-H 2 O 2 system at 652 nm for quantifying H 2 O 2 , which holds good linear relationship between 1 and 150 μM and has a detection limit of 0.61 μM. We also investigated the degradation of MB in the presence of Cu 2+ -HCNSs-COOH and H 2 O 2 , which can degrade 80.7% pollutants within 30 min. This research developed an unusual nanozyme for bioassays and water pollution treatment, which broadened the way for the rapid development of clinical diagnostics and water pollution treatment. Graphical abstract
研究领域:
纳米材料科学
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生物分析化学
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环境科学
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DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
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2021
·
IF 8.067
Yan-Cai Gao,Chong Wang,Chun-Xia Zhang,Hong-Wei Li,Yuqing Wu
In the present study, we have prepared glutathione capped gold and platinum alloy nanoclusters (Au-PtNCs) in a controlled way by employing the hydrothermal method and optimized through adjusting the ratio of raw materials, reaction temperature, and time. Compared with the corresponding monometallic gold and platinum nanoclusters, the alloy nanoclusters' catalytic activity is improved dramatically in the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H 2 O 2 . And the maximum velocity is calculated to be 106 × 10 −8 M · s −1 for TMB as substrate, being much better than that of other reported metallic nanoclusters and nanoparticles. Further study shows that the high catalytic activity mainly attributes to the synergistic effect of gold and platinum. Besides, they have been applied to determine H 2 O 2 in the presence of TMB, which shows high sensitivity with a limit of detection (LOD) at 100 nM. The proposed method has been used to determine H 2 O 2 in milk and contact lens solutions, which shows very good recovery and exhibits high practical application potential. Therefore, the present study provides a new type of alloy nanoclusters with high peroxidase-like activity, which will inspire more research interests on doping and alloying with Pt to improve the catalytic activity of metal nanoclusters.
研究领域:
纳米材料科学
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分析化学
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生物医学工程
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DOI
高影响因子
ELECTROCHIMICA ACTA
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2012
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IF 3.832
Xiangheng Niu,Hongli Zhao,Chen Chen,Minbo Lan
In the present study the electrocatalytic behavior of platinum nanoparticle-decorated multi-walled carbon nanotube clusters vertically aligned on a screen-printed gold nanofilm electrode substrate (SPGFE/MWCNTC/PtNP) toward hydrogen peroxide was intensively investigated. Oriented multi-walled carbon nanotube clusters (MWCNTC) were firstly well-organized onto the screen-printed gold film electrode (SPGFE) surface by covalent immobilization, and then platinum nanoparticles (PtNPs) dispersed on the MWCNTC structure were in situ synthesized by electrochemically reducing chloroplatinic acid. It was demonstrated that the MWCNTC/PtNP hybrid structure on the SPGFE substrate could significantly enhance the electrochemical reduction of hydrogen peroxide in neutral solutions. Compared with platinum nanoparticles loading on disordered multi-walled carbon nanotubes (SPGFE/MWCNT/PtNP), the proposed SPGFE/MWCNTC/PtNP exhibited more sensitive responses for hydrogen peroxide reduction. The fabricated electrode provided linear current responses for hydrogen peroxide in the concentration range from 5 to 2000 μM with a detection limit of 1.23 μM. The non-enzymatic sensor also offered good reproducibility and high selectivity for hydrogen peroxide detection. The carbon nanotube cluster/platinum nanoparticle hybrid system holds great promise as a scaffold to fabricate enzyme biosensors.
研究领域:
材料科学
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生物医学工程
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电化学
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DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2020
·
IF 7.489
Haohao Sun,Yan Gao,Narisu Hu,Yongxia Zhang,Chongshen Guo,Guanggang Gao,Zhuo Ma,Krasnyuk Ivan Ivanovich,Yunfeng Qiu
Peroxidase nanoenzymes exhibit a specific affinity toward substrates, thereby demonstrating application potential for realizing the colorimetric immunoassays of hydrogen peroxide (H 2 O 2 ), which can be further used as a probe for imaging cancer cells. To enhance the intrinsic peroxidase activity of molybdenum sulfide (MoS 2 ) nanomaterials , gold (Au) nanoparticles with an average diameter of approximately 2.1 nm were modified on a MoS 2 /carbon surface (denoted as MoS 2 /C-Au 600 ) via ascorbic acid reduction. MoS 2 /C-Au 600 can oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) to generate a blue oxidation product in the presence of H 2 O 2 ; this product exhibits peroxidase-like activities, superior to those of most existing MoS 2 -based nanoenzymes. Furthermore, MoS 2 /C-Au 600 exhibits a high detection capability for H 2 O 2 in the range of 1 × 10 −5 to 2 × 10 −4 mol/L (R 2 = 0.99), and the lowest detection limit is 1.82 µmol/L in a sodium acetate and acetic acid buffer solution . Steady state kinetics studies indicate that the catalytic mechanism is consistent with the ping-pong mechanism. Given its strong absorption peak at 652 nm in the visible region, MoS 2 /C-Au 600 can be used to image cancer cells due to the enhanced permeability and retention effect . Our findings demonstrate that the synergistic electronic coupling between multiple components can enhance the peroxidase activity, which can facilitate the development of an effective, facile, and reliable method to perform colorimetric immunoassays of H 2 O 2 and cancer cells.
研究领域:
生物化学
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纳米医学
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分析化学
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Yan Zhang,Tao Lin,Minghui Han,Min Hu,Yun Xu,Wei Huang,Fei Xiao,Anshun Zhao
In this work, we developed a new strategy to fabricate a series of transition metallic nanoparticles (NPs) embedded on B, N co-doped carbon nanotubes (CNTs) arrays modified flexible carbon fiber electrodes (M@BNCNTs/CF, M = Co, Fe, Ni) via facile inkjet printing assisted with chemical vapor deposition using Ionic liquid as solvent of printing ink and heteroatom dopants. Furthermore, Pt NPs via impregnation-thermal reduction process was anchored on the surface of Co@BNCNTs/CF (Pt–Co@BNCNTs/CF), which holds enhanced peroxidase-like activity and could be directly used as freestanding electrode to detect H 2 O 2 , exhibiting a low detection limit of 0.19 μM with wide linear range (0.5 μM–9.4 mM), and high sensitivity (1679 μA cm −2 mM −1 ). The excellent sensing performance of Pt–Co@BNCNTs/CF is attributed to the Pt, Co NPs anchored on CNTs with great catalytic activity, and the doping B, N would cause graphitic carbon with more defects to improve its inherent reactivity toward H 2 O 2 . Besides, CNTs arrays with high surface area also enlarge the exposure of active sites. Moreover, the Pt–Co@NBCNTs/CF microelectrode has been successfully applied in monitoring H 2 O 2 secreted from human colonic cancer cells and normal colonic epithelial cells, which could offer crucial data for distinguishing various cell types and identifying cancer cells from normal cells. This work opens a new horizon to fabricate flexible miniaturized sensing device for extracellular analysis and offers an extended strategy to fabricate other metallic NPs embedded in heteroatoms doped CNTs functionalized flexible fiber electrode, by choosing diverse metal ions and ILs as inkjet printing precursors.
研究领域:
生物传感器
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材料科学
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分析化学
·
癌症研究
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产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 43
·期 1
·146-153
·
IF 3.201
Haotian Zhu,Jing Du,Ying Lu,Fang Su,Yangguang Li
A new polyoxometalate (POM)-based organic–inorganic hybrid compound, {[(Zn(H2biim)2)3(P2W18O62)]·6H2O}n (1) (H2biim = 2,2′-biimidazole), has been hydrothermally synthesized and characterized by physico-chemical and spectroscopic methods. The structure of compound 1 exhibits a two-dimensional (2-D) network character, in which each Dawson-type polyoxoanion [P2W18O62]6− ({P2W18}) is connected with six [Zn(H2biim)2]2+ ({Zn(II)-H2biim}) complex fragments, while each {Zn(II)-H2biim} unit bridges two {P2W18} clusters, forming a 2-D network with a (6,3)-net topology. Compound 1 shows high enzyme-immobilizing capability for horseradish peroxidase (HRP). The maximum HRP load is 90.1 mg g−1 under optimal conditions. Furthermore, the immobilized enzyme (HRP/1) exhibits high catalytic activity and good stability, which can be applied to detect trace amounts of H2O2 in solution with a low detection limit of 3.11 × 10−3 mmol L−1.
研究领域:
生物化学
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材料科学
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DOI
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 74
·70124-70132
·
IF 3.289
T. M. Chen,J. Xiao,G. W. Yang
Inorganic artificial enzymes have been developed as potential candidates to naturally occurring enzymes, and three inorganic enzyme mimics, noble metals, metal oxides and carbon materials, have been reported so far. Here, we reported the inorganic enzyme mimic of nitride-based materials. We demonstrated that cubic boron nitride (c-BN) as an enzyme mimic showed intrinsic peroxidase-like activity towards classical colorimetric substrates in the presence of hydrogen peroxide (H2O2). The Michaelis–Menten kinetics studies indicated that the catalytic efficiency of c-BN is superior to its natural peroxidase counterparts. We also established that the peroxidase-like activity of c-BN is induced by catalyzing the decomposition of H2O2 and generating hydroxyl radicals (˙OH). Based on the color reaction, a strategy was developed for H2O2 and glucose quantitative detection with high sensitivity. A reactor was constructed by entrapping c-BN in a porous platform and the peroxidase mimic immobilization for removal of organic pollutants was successfully conducted. Additionally, c-BN can be re-used up to 5 times and retain its catalytic activity after incubation at extremes of pH and temperature. These findings open the door for the application of c-BN as a catalyst and the development of nitride-based materials in the enzyme-mimics field.
研究领域:
材料科学
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生物催化
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分析化学
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DOI
高影响因子
危险品化学品经营许可证（带存储）