Food Bioscience
·
2025
·
IF 5.9
Xueer Lin,Jiahui Zou,Guancheng Zhu,Linfeng Pang,Yuqi Chen,Yanyan Huang
To effectively overcome the shortcomings of traditional chromatographic and enzymatic methods for detecting organophosphorus pesticides, Cu-MOFs with inherent peroxidase (POD)-like activity were constructed for direct colorimetric detection of omethoate (OMT). Benefiting from the mesoporous and benzene ring structure, Cu-MOFs demonstrated excellent OMT enrichment capability. OMT exhibited a pronounced inhibitory effect on the POD-like activity of Cu-MOFs whereas other pesticides showed negligible interference, demonstrating the specific identification of OMT by Cu-MOFs. Experimental data and theoretical calculations indicated that OMT effectively bond to Cu-MOFs through Cu 2+ coordination and ligand binding. Owning to its enrichment, identification and catalytic abilities, OMT detection was achieved and the limit of detection (LOD) was 16 μg L −1 . Furthermore, a smartphone-assisted portable detection was established with a LOD of 79 μg L −1 . Validation experiments of cabbage samples confirmed its’ reliability for practical applications. Our system integrated the enrichment, identification, and detection of OMT within a single system, improving both sensitivity and operational convenience.
研究领域:
分析化学
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纳米技术
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环境科学
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生物传感器
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农药检测
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DOI
高影响因子
Foods
·
2022
·Vol. 11
·期 18
·2900
·
IF 5.561
Biao Zhang,Ruofan Zhou,Huiqi Zhang,Danfeng Cai,Xiaodong Lin,Yihan Lang,Yulou Qiu,Xuping Shentu,Zihong Ye,Xiaoping Yu
A smartphone colorimetric sensor based on the Pt@Au nanozyme was successfully developed for the visual and quantitative detection of omethoate in fruit and vegetables. The anti-omethoate antibody was conjugated on the surface of the Pt@Au nanozyme as a catalytic functional signal probe, and coating antigen conjugated on the surface of magnetic polystyrene microspheres (MPMs) was used as a separation capture probe. In the sensing system, when the catalytic functional signal probe was combined with a separation capture probe containing no omethoate, the visible blue color appeared with the addition of tetramethylbenzidine (TMB) chromogenic solution, and the maximum B value of the sensing system was obtained via the smartphone. With increasing concentrations of omethoate, the visualization of the sensing system decreased, and the B-value obtained via the smartphone dropped. Under optimal detection conditions, the omethoate could be detected in a linear range of 0.5–50 μg/L (R2= 0.9965), with a detection limit of 0.01 μg/L. The accuracy and reliability of the detection results of this colorimetric sensor were successfully confirmed by enzyme linked immunosorbent assay (ELISA) and gas chromatography. This colorimetric sensor provides a technical reference and potential strategy for the immunoassay of hazard factors in resource-scarce laboratories.Keywords:colorimetric sensor;Pt@Au nanozyme;visualized biosensing;smartphone-based quantitative detection;omethoate
研究领域:
分析化学
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食品安全检测
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纳米生物技术
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DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
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2015
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IF 4.513
Xiaowen Dou,Xianfeng Chu,Weijun Kong,Jiaoyang Luo,Meihua Yang
A nanomaterials-based novel molecular beacon has attracted growing attentions in fluorescent assays as many nanomaterials possess excellent quenching efficiency. In this work, a gold-based nanobeacon probe was established to detect organophosphorus pesticides for the first time. The constructed gold-based nanobeacon acted as a signal indicator and could display the decreasing of the intensity in the presence of targets, which competitively bound to single strand DNA . To achieve a high sensitive probe, some parameters including solution pH, temperature and reaction time were investigated and optimized. The gold-based nanobeacon probe assay was proved to be rapid and sensitive to achieve a detection limit of 0.035 μM for isocarbophos , 0.134 μM for profenofos , 0.384 μM for phorate and 2.35 μM for omethoate , respectively. The prepared nanobeacon effectively reduced the background and improved the detection sensitivity and selectivity . The probe is stable, easy to operate and does not need sophisticated instruments. These features makes the probe feasible for screening trace organophosphorus pesticides in real samples.
研究领域:
分析化学
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环境科学
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纳米技术
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农业科学
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DOI
PubMed
高影响因子
ANALYTICAL LETTERS
·
2022
·
IF 2.267
Dan Fei,Mengzhi Wang,Yujie Hou,Min Xie,Yaomin Zhou,Youhua Zhao,Lei Wu,Jun Xu
The indiscriminate use of organophosphorus pesticides poses a great threat to human health because of harmful substance bioaccumulation. We developed a rapid and reliable method for the simultaneous determination of chlorpyrifos, isocarbophos, methamidophos, omethoate, and triazophos in procine hair. The targets were separated by a C18 column (2.1 × 100 mm, 1.8 µm) and analyzed by electrospray ionization using the positive mode of a triple quadrupole mass spectrometer. The analytes had linear relationships from 1.0 to 100 µg/kg. The correlation coefficients were all greater than 0.998. The limits of detection ranged from 0.4 to 0.9 µg/kg, and the limits of quantification from 1.2 to 2.0 µg/kg. The recoveries were from 76.1% to 106.2%. The intra-assay and inter-assay precision rates were from 1.6% to 4.4% and 2.3% to 6.9%, respectively. The intra-day and inter-day accuracy ranged from 2.5% to 9.3% and 3.2% to 8.9%, respectively. This method was a valid tool to monitor organophosphorus pesticides in breeding and livestock products.
研究领域:
分析化学
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环境科学
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兽医学
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2013
·
IF 2.906
Qin Zhao,Qian Lu,Qiong-Wei Yu,Yu-Qi Feng
This article presents a novel application of dispersive microextraction based on “magnetic water” (m-water) for the purification of organophosphorus pesticides (methamidophos, omethoate, monocrotophos) from cold-pressed vegetable oils. In the present study, a trace amount of water (extractant) was adsorbed on bare Fe3O4 by hydrophilic interaction to form m-water. Rapid extraction can be achieved while the m-water is dispersed in the sample solution with the aid of a vigorous vortex. After extraction, the analyte-adsorbed m-water can be readily isolated from the sample solution by a magnet, which could greatly simplify the operation and reduce the whole pretreatment time. Several parameters affecting the extraction efficiency were investigated, and under the optimized conditions, a simple and effective method for pesticide analysis was established by coupling with gas chromatography/mass spectrometry (GC/MS). The linearity range of the proposed method was 2–100 ng/g with satisfactory correlation coefficients (R) of 0.9997–0.9998, and the limits of quantification (LOQ) for the target compounds were in the range of 0.70–1.27 ng/g. In addition, the reproducibility was obtained by evaluating the intra- and interday precisions with relative standard deviations (RSDs) less than 7.2% and 6.5%, respectively. Finally, the established “magnetic water” microextraction method was successfully applied for the determination of pesticide residues in several kinds of cold-pressed vegetable oils.
研究领域:
分析化学
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环境科学
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食品科学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2021
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IF 7.46
Yisong Zhao,Xiaolu Li,Jianmin Chen,Xiong Lu,Yunxia Yang,Dandan Song,Faming Gao
Efficient and sensitive electrochemical biosensors are of great significance for on-site measurement and evaluation of pesticides in the environment, and various functional nanomaterials have been designed and developed to achieve this goal. Herein, a kind of three-dimensional holey hierarchical cobalt-based bimetallic oxides randomly assembled by micro-mesoporous ultrathin nanosheets has been exploited. This unique porous hierarchical structure and bimetal composition not only possesses excellent electron pathways and consequent an enhanced electro-conductivity and better catalytic activity , but also exhibits high electroactive surface area and more vacant sites for enzyme immobilization . Along with the highly conductive and biocompatible layered graphdiyne , the 3D porous Co-based bimetallic oxides have been applied to construct an electrochemical AChE biosensor for monitoring dipterex and omethoate (as model OPs pesticides). As a result, the reported biosensor demonstrates superior sensing performance, for example, the wide detection range of 3.88 × 10 −12 –3.88 × 10 −6 M for diptetex and 4.69 × 10 −13 –4.69 × 10 −7 M for omethoate , together with the relative low detection limit of 0.36 pM and 0.033 pM (S/N = 3), respectively. Additionally, the biosensor is feasible in real samples, and the recoveries (94.8–105.1%) are satisfactory. And a possible mechanism has also been proposed. This study offers a new strategy to modulate the property of metal oxides , which make them more attractive in electrochemical sensors .
研究领域:
材料科学
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电化学
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环境科学
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纳米技术
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生物传感器
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DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2022
·Vol. 414
·期 24
·7277-7289
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IF 4.478
Wang Min,Hou Li,Chen Xiaoyu,Lin Tianran
A simple homogeneous photoelectrochemical (PEC) sensing platform based on an alkaline phosphatase (ALP)–mediated pesticide assay was established for the sensitive detection of omethoate (OM). The Bi 2 S 3 @Bi 2 Sn 2 O 7 heterojunction was used as a photoactive material to provide stable background photocurrent signals. The inhibition of OM on ALP and PEC determination was carried out in the homogeneous system. In the absence of OM, dephosphorylation of L-ascorbic acid 2-phosphate trisodium salt (AAP) was catalyzed by ALP to produce the enzyme-catalyzed product (L-ascorbic acid, AA). AA, as an electron donor, could capture photogenerated holes on the Bi 2 S 3 @Bi 2 Sn 2 O 7 heterojunction, thus inhibiting the recombination of electron holes to achieve an increase of the photocurrent signal. When the OM was introduced, the enzyme activity of ALP was reduced due to the organophosphorus pesticides (OPs)–based enzyme inhibition, and the AA produced by catalytic hydrolysis was also reduced, thus reducing the photocurrent signal. Compared with the traditional PEC sensor for OPs, this homogeneous PEC sensor avoided immobilization procedures, covalent labeling, separation, and the steric hindrance effect caused by immobilized biomolecules, which achieved high recognition efficiency and caused a reduction in analysis time. Additionally, an ALP-mediated pesticide assay for the determination of OPs with a simplified experimental process further improved the stability and reproducibility of the PEC sensor. The PEC sensor showed high sensitivity to the target OM within a dynamic range of 0.05 ~ 500 ng mL −1 , and the detection limit was 0.0146 ng mL −1 . Additionally, the PEC biosensing system showed good selectivity and anti-interference ability, and exhibited a satisfactory result in spinach and mustard samples. Graphical abstract A homogeneous PEC biosensor based on ALP inhibition strategy was constructed for OM detection in vegetable samples via Bi 2 S 3 @Bi 2 Sn 2 O 7 heterojunction as the photoactive substrate material
研究领域:
分析化学
·
环境科学
·
材料科学
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生物传感器
·
农药分析
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）