ACS Omega
·
2020
·
IF 2.87
Peide Zhu,Ying Gan,Kunpeng Lin,Chen Lin,Shanshan Li,Shuling Yu,Jiahua Shi
The pH/redox dual-sensitive fluorescent carbon dots (pHRCDs) with the fluorescence quantum yield of 16.97% were synthesized by the pyrolysis of l-glutamic acid (l-glu) and dopamine (DA). Compared with the quantum dot (QD)–dopamine conjugate, when the pH value of the solution was changed from neutral to alkaline, the pHRCDs exhibited unique optical phenomenon including red-shift of fluorescence peak and the fluorescence intensity first decreasing from pH 7 to 10 and then increasing from pH 10 to 13. The pHRCDs could be developed for a discriminative and highly sensitive dual-response fluorescent probe for the detection of oxidized glutathione (GSSG) and ascorbic acid (AA) activity in human blood. Under the optimized experimental conditions, the dual-response fluorescent probe can detect GSSG and AA in the linear range of 1.2–3.6 and 27–35 μM with the detection limits of 0.1 and 3.1 μM, respectively. In addition, the pHRCDs demonstrated low cytotoxicity and good biocompatibility, which can be well applied to in vitro cell imaging, and the pHRCDs/GSH fluorescence system has been successfully developed for the detection of AA in real samples.
研究领域:
分析化学
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生物医学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Li Qiu,Yanbao Zhao,Binjie Li,Zhihua Wang,Liuqin Cao,Lei Sun
For the purpose of reducing the adverse effect of antitumor drugs, a novel type of protease/redox/pH stimuli-responsive fluorescent porous silica (pSiO 2 ) nanocarrier was designed. In this system, oxidized glutathione (GSSG), a kind of peptide with disulfide linkage, was grafted onto the pSiO 2 nanospheres (NSs) via amido bonds. After that, acid-decomposable, luminescent ZnO quantum dots (QDs) were introduced to seal the nanopores of pSiO 2 NSs via covalent attachment. The ZnO QDs behave as a dual-purpose entity that not only act as a fluorescence probe for drug-release monitoring but also play the role of gatekeeper that controls the drug release. The obtained ZnO-gated pSiO 2 -GSSG NSs delivery system would achieve minimized premature release under a physiological environment. The controlled release of the drug from the ZnO-gated delivery system was realized by the acidic dissolution of ZnO QDs, reduction of disulfide bonds and cleavage of the amino bonds, leading to ZnO falling off from the surface of pSiO 2 NSs. The constructed ZnO-gated pSiO 2 -GSSG NSs delivery system has promising applications in site-specific drug release for tumor chemotherapy.
研究领域:
药剂学
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生物医学工程
·
肿瘤学
✓ 已验证
DOI
高影响因子
Analytical Methods
·
2022
·Vol. 14
·期 48
·5034-5040
·
IF 3.532
Guoli Wu,Yongdan Zhao,Xiaofang Li,Xiaolin Lu,Tingli Qu
Glutathione (GSH) is a polypeptide with important physiological functions. Real-time and accurate detection of GSH is of great significance for clinical diagnosis, disease treatment and pathogen detection. A fluorescent nanosensor based on composite core–shell nanoparticles for the highly selective detection of GSH is reported. In the cores, the fluorescence of rhodamine b was quenched by using gold nanoparticles (AuNPs), and GSH could competitively combine with AuNPs to cause rhodamine b to fall off, thereby recovering the fluorescence. In the shell part, molecularly imprinted materials using oxidized glutathione (GSSG) as a pseudotemplate provide GSH/GSSG specific pores and improve the specificity and anti-interference ability of the sensor. The GSH sensor has a detection range of 0–100 μM and limit of detection (LOD) of 0.18 μM, and robust sensing performance in fetal bovine serum, indicating its great potential for clinical diagnosis.
研究领域:
分析化学
·
纳米技术
·
生物医学工程
·
临床诊断
✓ 已验证
DOI
高影响因子
ACS Nano
·
2025
·
IF 16
Ziyu Zhang,Haolin Li,Jing Xu
The practical application of aqueous zinc-ion batteries (AZIBs) requires addressing the key challenge of temperature range limitations, facing thermodynamics dominated corrosion at high temperatures and kinetics dominated mass transfer obstruction at low temperatures. Although these temperature-related failure mechanisms have been extensively studied, the corrosion of dissolved oxygen (DO) remains frequently overlooked, necessitating further research of wide-temperature failure mechanisms in AZIBs. Herein, we demonstrate that DO corrosion occurs over a wide-temperature range and significantly exacerbates Zn corrosion and byproducts formation, surpassing the well-recognized proton corrosion. We introduce antioxidant glutathione (GSH) as an electrolyte additive to address the corrosion caused by DO and proton. Coexisting glutathione/oxidized glutathione redox couple can spontaneously capture DO and eliminate the generated H2. Moreover, GSH disrupts the intrinsic H-bond networks, depresses the electrolyte freezing point, and enhances the low-temperature mass-transfer kinetics. A self-healing hybrid solid electrolyte interphase (SEI) can also be formed during battery operation, which suppresses dendrite growth. Consequently, the Zn||Zn symmetric cell with GSH-modified electrolyte achieves an extended lifespan of up to 5000 h at −10 °C, 1700 h at 25 °C, and 2500 h at 40 °C. This work reveals a dynamic chemical self-deoxygenation/self-dehydrogenation strategy to design a non-corrosive and dendrite-free Zn anode from −10 to 40 °C for stable AZIBs.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2023
·Vol. 148
·期 10
·2375-2386
·
IF 4.2
Yuwei Du,Lei Cao,Xinlu Li,Tongtong Zhu,Ruhong Yan,Wen-Fei Dong,Li Li
Carbon dots (CDs) with red fluorescence emission are highly desirable for use in bioimaging and trace- substance detection, with potential applications in biotherapy, photothermal therapy, and tumor visualization. Most CDs emit green or blue fluorescence, thus limiting their applicability. We report a novel fluorescent detection platform based on high-brightness red fluorescence emission carbon dots (R-CDs) co-doped with nitrogen and bromine, which exhibit pH and oxidized L-glutathione (GSSG) dual-responsive characteristics. The absolute quantum yield of the R-CDs was as high as 11.93%. We discovered that the R-CDs were able to detect acidic pH in live cells and zebrafish owing to protonation and deprotonation. In addition, GSSG was detected in vitro over a broad linear range (8–200 μM) using the R-CDs with excitation-independent emission. Furthermore, cell imaging and bioimaging experiments demonstrated that the R-CDs were highly cytocompatible and could be used as fluorescent probes to target lysosomes and nucleolus. These studies highlight the promising prospects of R-CDs as biosensing tools for bioimaging and trace-substance detection applications.
研究领域:
材料科学
·
纳米技术
·
生物医学工程
·
分析化学
·
生物传感器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2013
·
IF 4.387
Shuyun Zhu,Xian-en Zhao,Wei Zhang,Zhongyuan Liu,Wenjing Qi,Saima Anjum,Guobao Xu
Fluorescent silver nanoclusters stabilized by DNA (DNA-AgNCs) exhibit distinct response rates to thiol and disulfide. Glutathione reductase can catalyze the reduction of the oxidized glutathione (GSSG) quickly to reduced glutathione (GSH) in the presence of β-nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate (NADPH). Consequently, DNA-AgNCs can serve as a new fluorescent platform for assaying the glutathione reductase (GR) activity. This newly proposed assay has a high sensitivity and a good selectivity toward GR. The GR activity can be detected in the range of 0.2–2.0 mU mL −1 with a minimum detectable concentration of 0.2 mU mL −1 . Pepsin, lysozyme, trypsin, avidin, thrombin, myoglobin, and BSA have little effect on the fluorescence intensity of DNA-AgNCs. The GR activity assay is successfully used to monitor the inhibition of GR activity by a typical inhibitor 1,3-bis(2-chloroethyl)-1-nitrosourea.
研究领域:
生物化学
·
分析化学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Environmental Science-Nano
·
2023
·Vol. 10
·期 12
·3389-3400
·
IF 7.3
Shuoli Ma,Wen-Xiong Wang
Marine hydrosphere is in grave danger if extreme weather conditions, such as high temperatures and heat waves, become the norm. The functional characteristics of nano Zn oxides (nZnO) might provide a “booster substance” that helps marine species to endure environmental stress. In the present study, physiological indicators and simulated severe environments were used to evaluate the antioxidative ability of nZnO in marine rabbitfish Siganus fuscescens, with an aim to help them resist extreme environmental stress. Rabbitfish were exposed to different diets with ZnSO4 and nZnO at the optimum requirement level (Zn content of 62.5 μg g−1), and then challenged with a low dissolved oxygen concentration and higher temperature. We demonstrated that dietary nZnO promoted the anti-oxidation defense system of the rabbitfish by improving the activities of the antioxidant enzymes, i.e., catalase (CAT), superoxide dismutase (SOD), and the reduced glutathione/oxidized glutathione (GSH/GSSG) ratio without tissue damage. Compound stressors reduced the median survival duration of the rabbitfish from 4.25 h to 2.5 h (dietary exposure) and 9 h to 5.75 h (dietary nZnO exposure) compared to single environmental stress. nZnO downregulated the HIF gene expression and increased the prolyl hydroxylase (PHD) activity in the liver of the rabbitfish. After 10 h of extreme environmental exposure, the gene expression results indicated that nZnO inhibited the NF-κB/IL-1β/IL-6 pathway and TNF-α/Bcl-2/Bax/caspase 9 pathway and activated the akt/mTOR/elovl5 and elovl8 pathway of the rabbitfish to maintain the resilience of the fish under the extreme environment. These results strongly suggested that nZnO may improve the anti-oxidation ability of fish to resist extreme environments.
研究领域:
海洋生物学
·
环境科学
·
水产养殖
·
分子生物学
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产品关联
DOI
高影响因子
Pharmaceutics
·
2024
·Vol. 16
·期 9
·1178
·
IF 4.9
Xiao Duan,Qiang Wang,Yue Wang,Xinping Liu,Manman Lu,Zhifang Li,Xuelian Jiang,Jingquan Ji
Using an endogenous carrier is the best method to address the biocompatibility of carriers in the drug delivery field. Herein, we prepared a glutathione-responsive paclitaxel prodrug micelle based on an endogenous molecule of L-glutathione oxidized (GSSG) for cancer therapy using one-pot synthesis. The carboxyl groups in L-glutathione oxidized were reacted with the hydroxyl group in paclitaxel (PTX) using the catalysts dicyclohexylcarbodiimide (DCC) and 4-dimethylaminopyridine (DMAP). Then, the amino-polyethylene glycol monomethyl ether (mPEG-NH2) was conjugated with GSSG to prepare PTX-GSSG-PEG. The structure of PTX-GSSG-PEG was characterized using infrared spectroscopy (FT-IR), nuclear magnetic resonance spectroscopy (NMR), and mass spectrometry (MS). The drug release kinetics of PTX within PTX-GSSG-PEG were quantified using ultraviolet spectroscopy (UV-Vis). The size of the PTX-GSSG-PEG micelles was 83 nm, as evaluated using dynamic light scattering (DLS), and their particle size remained stable in a pH 7.4 PBS for 7 days. Moreover, the micelles could responsively degrade and release PTX in a reduced glutathione environment. The drug loading of PTX in PTX-GSSG-PEG was 13%, as determined using NMR. Furthermore, the cumulative drug release rate of PTX from the micelles reached 72.1% in a reduced glutathione environment of 5 mg/mL at 120 h. Cell viability experiments demonstrated that the PTX-GSSG-PEG micelles could induce the apoptosis of MCF-7 cells. Additionally, cell uptake showed that the micelles could distribute to the cell nuclei within 7 h. To sum up, with this glutathione-responsive paclitaxel prodrug micelle based on the endogenous molecule GSSG, it may be possible to develop novel nanomedicines in the future.
研究领域:
药学
·
纳米技术
·
癌症研究
·
药物递送系统
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2022
·
IF 12.545
Xia Liu,Lei Qin,Jie Yu,Wentao Sun,Jianhong Xu,Chun Li
During industrial fermentation , microbial cell factories are usually confronted with environmental or metabolic stresses, leading to the imbalance of intracellular redox and the reduction of cell metabolic capacity. Here, we constructed the genetically encoded redox biosensor system (RBS) based on redox-sensitive fluorescent proteins to detect redox metabolites , including reactive oxygen species (ROS), oxidized glutathione , NADH, and NADPH in Saccharomyces cerevisiae . The functional biosensors were quantitatively characterized and the orthogonal redox biosensor system (oRBS) was designed for detecting multiple redox metabolites. Furthermore, the compartment targeted redox biosensor system (ctRBS) was constructed to detect ROS and NADPH, revealing the distribution and spatiotemporal dynamics of ROS in yeast under various stress conditions. As a proof-of-concept, RBS was applied to evaluate the redox states of engineered yeast with stress resistance and heterogenous triterpene synthesis in vivo , elucidating the redox balance significantly affecting the growth and production phenotypes. The RBS in this study allowed the exploration of the diversity of compartmental redox state and real-time monitoring of the production process of yeast, providing a reliable and effective approach for accurate and in-depth profiling of bottlenecks of yeast cell factories.
研究领域:
生物技术
·
微生物学
·
分子生物学
·
系统生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Mintong Tian,Zehua Li,Purui Yu,Meng Zhou,Mingming Li,Guanghao Li,Qipeng Yuan
Glucosinolates (GLs) serve as crucial precursors for bioactive isothiocyanates (ITCs). As a GL, glucoraphenin (GRE) exhibits significant potential for diverse applications. Despite being deemed stable, massive GRE decomposed during cooking and processing. To further enhance the application of GRE, the decomposition mechanisms were investigated by initiating the decomposition reactions through manipulation of concentration, temperature, pH value, solvents, and reducing agents. The decomposition products were identified using NMR and LC-MS to further elucidate the decomposition mechanisms. The solvolysis initiated the decomposition, with the decomposition products being determined by the selective removal of either glucose or thioglucose, which was influenced by exogenous factors. The removal of thioglucose disabled the formation of ITCs, and nitrile was generated through a redox mechanism involving the removal of HS-OH. These mechanisms helped elucidate the effects of various factors on GRE decomposition and provided insights for effective utilization of GRE during the cooking or processing.
研究领域:
植物化学
·
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）