ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Mengxiao Zhong,Maoqiang Chi,Yun Zhu,Ce Wang,Xiaofeng Lu
Controllable fabrication of novel and uniform noble metal nanoparticles on a specific support with a superior catalytic or electrocatalytic performance is of significantly importance for practical applications. In this report, we demonstrated an effective way to fabricate uniform thin-walled Pd/polypyrrole (PPy) hollow nanotubes . The prepared Pd/PPy hybrid nanotubes exhibited an excellent peroxidase-like activity to oxidize a typical peroxidase substrate such as 3,3′,5,5′-tetramethylbenzidine in comparison with traditional Pd/C and Pd black catalysts. The outstanding catalytic activity of the Pd/PPy hybrid nanotubes for peroxidase mimicking could be resulting from their unique hollow characteristic and an interfacial effect between PPy and Pd components. Based on the favorable catalytic property of the Pd/PPy hybrid nanotubes, a convenient and rapid colorimetric way to sensitively determine ascorbic acid has been presented. The detection limit was around 0.062 μM and an excellent selectivity was also achieved. The developed detection system in this study could be extended to the fields of bioscience and biotechnology with promising prospects.
研究领域:
生物化学
·
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Weiguo Wang,Rongkai Du,Changzhi Dong,Jinwu Yan,Lei Zhang
A sensitive enzyme-linked immunosorbent assay (ELISA) has been developed based on the dual catalysis of dendritic Pd-Pt porous nanoparticles and horseradish peroxidase (HRP). The Pd-Pt porous nanoparticles, for the first time, were used to immobilize HRP. As a proof-of-concept demonstration, HRP functioned Pd-Pt porous nanoparticles were applied in hCG detection with a detection limit of 0.07 IU L −1 . The linear intervals is 0.1–6.4 IU L -1 . The Pd-Pt-HRP possesses a better sensitivity than the traditional BA-ELISA. Furthermore, the precision and reproducibility and selectivity were assessed to be eligible for the hCG monitoring.
研究领域:
生物化学
·
医学诊断
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Yachao Zhang,Yan Gao,Xueli Zhang,Huiming Wang,Tian Xia,Congcong Bian,Shuang Liang,Xiaozhi Tang,Xia Wang
The amplification of the electrochemical signal is crucial to improving the sensing performance of an immunosensor. In this study, a signal amplification strategy based on the co-catalysis of horseradish peroxidase (HRP) and nanoporous gold (NPG) was used to fabricate an electrochemical immunosensor for the determination of Hepatitis B e antigen (HBeAg). For the resulting immunosensor, HRP catalyzed catechol and thus served as a signal label of secondary antibody. NPG with unique properties was selected as a carrier for capture antibody immobilization. Further, NPG had an efficient electrocatalytic activity towards catechol (the substrate of HRP), which thus significantly enhanced the electrochemical signal produced by the HRP-labeled secondary antibody. Based on the co-catalysis of HRP and NPG, the immunosensor exhibited a good linear relationship between peak current density and HBeAg concentration in a wide HBeAg detection range from 1 pg mL −1 to 1 ng mL −1 with a high sensitivity of 23.51 μA mL ng −1 cm −2 as well as a low detection limit of 0.064 pg mL −1 (S/N = 3). Additionally, the immunosensor presented a strong anti-interference capability against human serum, human serum/dopamine, human serum/tryptophan, human serum/NO 2 − , human serum/D-glucose, and human serum/IgG. For the detection of HBeAg in human serum, the concentrations detected by the immunosensor were in good agreement with the standard concentrations that added in the human serum samples. These unique properties potentially make the proposed immunosensor a good choice for HBeAg detection.
研究领域:
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
·
2014
·
IF 2.745
Teng Zhang,Xiao-Ling Xu,Yi-Ning Jin,Jian Wu,Zhi-Kang Xu
Carbon nanotubes are widely applied in electronics, which offers great potential for enhancing the activity of redox enzyme. In this work, polyimide (PI) was blended with multiwalled carbon nanotubes (MWCNTs) and then electrospun into nanofibers for the immobilization of horseradish peroxidase (HRP), a redox enzyme. SEM and TEM were used to characterize the surface morphology of the PI/MWCNTs nanofibers and to analyze the protruding parts induced by the blending of MWCNT. The results indicate that, compared to the virgin PI nanofibers, the blending of MWCNTs increases the retention activity of immobilized HRP from 2.38% to 12.50%. Circular dichroism and UV–vis molecular absorption spectrometry were used to explore the interactions between HRP and MWCNTs . It seems that MWCNTs increase the enzyme activity by the facilitation of electron transfer among the catalytic intermediates “HRP I”, “HRP II” and MWCNTs.
研究领域:
生物化学
·
纳米生物技术
·
酶工程
·
生物材料
·
分析化学
✓ 已验证
DOI
高影响因子
Small
·
2018
·Vol. 14
·期 31
·1801680
·
IF 9.598
Jiangjiang Zhang,Wenshu Zheng,Xingyu Jiang
Chemical regulation of enzyme-mimic activity of nanomaterials is challenging because it requires a precise understanding of the surface chemistry and mechanism, and rationally designed applications. Herein, Ag + -gated peroxidase activity is demonstrated by successfully modulating surface chemistry of cetyltrimethylammonium bromide-capped gold nanoparticles (CTAB-AuNPs). A surface blocking effect of long-chain molecules on surfaces of AuNPs that inhibit peroxidase activity of AuNPs is found. Ag + ions can selectively bind on the surfaces of AuNPs and competitively destroy CTAB membrane forming Ag + @CTAB-AuNPs complexes to result in enhanced peroxidase activity. Ag + @CTAB-AuNPs show the highest peroxidase activity compared to similar-sized citrate-capped and ascorbic acid-capped AuNPs. Ag + @CTAB-AuNPs can potentially develop into analyte-responsive systems and exhibit advantages in the optical sensing field. For example, the Ag + @CTAB-AuNPs system shows an enhanced sensitivity and selectivity for acetylcholinesterase activity sensing compared to other methods.
研究领域:
纳米生物技术
·
分析化学
·
酶学
·
生物传感器
·
分子诊断
·
转化医学
·
药物代谢
✓ 已验证
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2011
·
IF 3.723
Hui Wang,Yuming Huang
Prussian-blue (PB)-modified γ-Fe 2 O 3 magnetic nanoparticles (PBMNPs) were successfully synthesized based on electric interactions between negatively charged [Fe(CN) 6 ] 4− and positively charged γ-Fe 2 O 3 nanoparticles. The in situ PB coating was generated by the coordinating reaction between the adsorbed [Fe(CN) 6 ] 4− and the ferric ions on the surface of γ-Fe 2 O 3 NPs. The as-prepared PBMNPs were characterized by FT-IR, XRD, TEM, and used to remove organic pollutants from aqueous solution, namely, using methylene blue (MB) as model compound. The experimental results showed that the target compound could be removed efficiently from solution over a wide pH range from 3 to 10 in the presence of PBMNPs as peroxidase-like catalyst and H 2 O 2 as oxidant. Under optimal conditions, MB could be removed completely after 120 min of reaction at 298 K; the chemical oxygen demand (COD) removal efficiency and the total organic carbon (TOC) abatement efficiency were 53.6% and 35%, respectively. Furthermore, the PBMNPs catalysts showed high magnetization, temperature tolerance, long-term storage and operational stability, and they could be readily separated from solution by applying an external magnetic field. Finally, a possible reaction mechanism for MB degradation was also discussed.
研究领域:
环境科学
·
纳米材料学
·
催化化学
·
水处理技术
·
环境功能材料
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）