CNS Neuroscience & Therapeutics
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2022
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IF 7.035
Jing Li,Jianhao Liang,Meiqin Zeng,Kaijian Sun,Yunhao Luo,Huaping Zheng,Feng Li,Wen Yuan,Hongwei Zhou,Junshan Liu,Haitao Sun
Introduction White matter injury (WMI) significantly affects neurobehavioral recovery in intracerebral hemorrhage (ICH) patients. Gut dysbiosis plays an important role in the pathogenesis of neurological disorders. Oxymatrine (OMT) has therapeutic effects on inflammation-mediated diseases. Whether OMT exerts therapeutic effects on WMI after ICH and the role of gut microbiota involved in this process is largely unknown. Methods Neurological deficits, WMI, gut microbial composition, intestinal barrier function, and systemic inflammation were investigated after ICH. Fecal microbiota transplantation (FMT) was performed to elucidate the role of gut microbiota in the pathogenesis of ICH. Results OMT promoted long-term neurological function recovery and ameliorated WMI in the peri-hematoma region and distal corticospinal tract ( CST ) region after ICH. ICH induced significant and persistent gut dysbiosis, which was obviously regulated by OMT. In addition, OMT alleviated intestinal barrier dysfunction and systemic inflammation. Correlation analysis revealed that gut microbiota alteration was significantly correlated with inflammation, intestinal barrier permeability, and neurological deficits after ICH. Moreover, OMT-induced gut microbiota alteration could confer protection against neurological deficits and intestinal barrier disruption. Conclusions Our study demonstrates that OMT ameliorates ICH-induced WMI and neurological deficits by modulating gut microbiota.
研究领域:
神经病学
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肠道微生物学
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分子药理学
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神经免疫学
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转化医学
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HUMAN & EXPERIMENTAL TOXICOLOGY
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2022
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IF 3.247
Shengwei Li,Guangqing Feng,Min Zhang,Xing Zhang,Jihong Lu,Chenyahui Feng,Fangshi Zhu
Objective: Due to its well-known anti-inflammatory property, oxymatrine (OMT) has received more attention on the aspect of treating ulcerative colitis. Although efforts have been undertaken to understand the therapeutic mechanism of OMT on ulcerative colitis (UC), the remedial principle is still ambiguous. Numerous studies have shown that TLR9/Myd88/NF-κB signal pathway played a key role in the pathogenesis of UC. Moreover, TLR9/Myd88/NF-κB signal pathway is a part of the most important pathways for regulating the immune response.Methods: We explored the influence of OMT with different dosages on UC by establishing a 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis model. Moreover, the participation of TLR9/Myd88/NF-κB signal pathway and whether OMT protects against UC though targeting this pathway are further studied.Results: Our data revealed that OMT could significantly relieve the symptom of TNBS-induced colitis in rats by reactivating the tight junction protein and, more important, by inhibiting the activation of TLR9/Myd88/NF-κB pathway and protein expression levels of its downstream inflammatory factors.Conclusion: OMT could relieve colitis in rat models by impacting tight junction proteins’ TLR9/Myd88/NF-κB signal pathways and activity.
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Cancer Management and Research
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2020
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IF 2.886
Yanshi Wang,Shaojie Yang,Shanshan Zhang,Xin Wu
Purpose To evaluate the anti-tumor effects of oxymatrine in vulvar squamous cell carcinoma (VSCC) cells and to explore the underlying mechanisms. Methods We selected SW962 and A431 VSCC cell lines. Cell proliferation was examined using MTT assay. Cell cycle and apoptosis were detected using flow cytometry. Migration and invasion were evaluated using transwell and wound-healing assays. The relevant protein expression and signaling pathways were analyzed using Western blotting. Results Oxymatrine inhibited the proliferation of SW962 and A431 VSCC cells in a time- and dose-dependent manner. Oxymatrine induced cell cycle arrest in the G2/M phase by increasing the protein expression of P21 and decreasing levels of cyclin B1 and CDC2. Oxymatrine upregulated the expression of cleaved-caspase 3 and BAX and downregulated the expression of BCL2, which led to an increase in apoptosis. Oxymatrine also suppressed the migration and invasion of SW962 and A431 cells by reducing levels of MMP2 and MMP9. After treatment with oxymatrine or a RAS inhibitor (salirasib), expression levels of RAS, p-RAF, p-MEK, p-ERK, C-MYC, and MMP2 were reduced. When TGF-β1 was used to stimulate SW962 and A431 cells, the expression of the above proteins increased; this increase was reversed by using oxymatrine or salirasib again. Conclusion Oxymatrine inhibits proliferation and migration of VSCC cells by blocking the RAS/RAF/MEK/ERK pathway.
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Frontiers in Pharmacology
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2020
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IF 4.225
Jin Yinshan,Liu Jiannan,Liu Yadong,Liu Yang,Guo Guiying,Yu Shiliang,An Ruihua
Aims: Oxymatrine (OMT) has been identified to possess immunomodulatory, antiinflammatory and anticancer properties. This study aimed to investigate its precise function and the underlying molecular mechanisms in renal cell carcinoma progression.Methods: The antineoplastic effect of oxymatrine was investigated by CCK-8 assay, cell cycle analysis, apoptosis assay, wound healing experiment, transwell assay, and drug-sensitivity analysis in renal cancer cells following oxymatrine treatment. The modulation of oxymatrine on β-catenin was analyzed through western blot and immunofluorescence assay. β-catenin overexpression was employed to determine the key role of β-catenin in oxymatrine-inhibited renal cell carcinoma in vitro. In addition, animal model was established to investigate the effect of oxymatrine on tumor growth in vivo.Results: Oxymatrine inhibited renal cell carcinoma progression in vitro, including cell proliferation, apoptosis, migration, invasion and chemotherapy sensitivity. Further mechanistic studies demonstrated that oxymatrine exerted its antineoplastic effect through suppressing the expression of β-catenin. Moreover, in nude mice model, oxymatrine exhibited remarkable inhibition of tumor growth, which was consistent with our in vitro results.Conclusions: Our findings illuminate oxymatrine as an effective antitumor agent in renal cell carcinoma, and suggest it a promising therapeutic application in renal cell carcinoma treatment.
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INTERNATIONAL IMMUNOPHARMACOLOGY
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2023
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IF 5.6
Chen He,Wenjing Wang,Guoli Wei,Yuqing Wang,Yingjie Wei,Jing Wang,Zhenhai Zhang
Hepatic fibrosis (HF) is a challenging clinical problem. Both sodium alginate (SA) and oxymatrine (OM) can be used to treat HF; however, the influence of viscosity on the therapeutic efficacy of sodium alginate is currently unknown. This study used a CCl 4 -induced HF mouse model to screen the specifications and doses of SA and investigate its therapeutic effects on HF in combination with OM. Sodium alginate of different viscosities ameliorated HF in mice, with 232 mPa·s SA delivered at a dose of 100 mg/kg showing remarkable therapeutic effect, characterized by reduced aspartate transaminase/alanine transaminase levels, reduced expression of α-SMA, collagen I, and other related genes, and increased abundance of beneficial intestinal probiotics such as Lactococcus and Blautia . The combination treatment further improved other related indices and increased the abundance of Phascolarctobacterium and Oscillospiraceae. These results suggest that the oral administration of SA may improve HF via the “gut-liver axis” based on the gut microbiota and has potential clinical applications.
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JOURNAL OF FISH DISEASES
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2023
·Vol. 46
·期 9
·977-986
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IF 2.5
Fa-Li Zhang,Liu Yang,Wei-Hao He,Ling-Jun Xie,Fei Yang,Ying-Hui Wang,Ai-Guo Huang
Streptococcosis disease caused by Streptococcus agalactiae (Group B Streptococcus, GBS) results in a huge economic loss of tilapia culture. It is urgent to find new antimicrobial agents against streptococcosis. In this study, 20 medicinal plants were evaluated in vitro and in vivo to obtain medicinal plants and potential bioactive compounds against GBS infection. The results showed that the ethanol extracts of 20 medicinal plants had low or no antibacterial properties in vitro, with a minimal inhibitory concentration ≥256 mg/L. Interestingly, in vivo tests showed that 7 medicinal plants could significantly inhibit GBS infection in tilapia, and Sophora flavescens (SF) had the strongest anti-GBS activity in tilapia, reaching 92.68%. SF could significantly reduce the bacterial loads of GBS in different tissues (liver, spleen and brain) of tilapia after treated with different tested concentrations (12.5, 25.0, 50.0 and 100.0 mg/kg) for 24 h. Moreover, 50 mg/kg SF could significantly improve the survival rate of GBS-infected tilapia by inhibiting GBS replication. Furthermore, the expression of antioxidant gene cat , immune-related gene c-type lysozyme and anti-inflammatory cytokine il-10 in liver tissue of GBS-infected tilapia significantly increased after treated with SF for 24 h. Meanwhile, SF significantly reduced the expression of immune-related gene myd88 and pro-inflammatory cytokines il-8 and il-1β in liver tissue of GBS-infected tilapia. The negative and positive models of UPLC-QE-MS, respectively, identified 27 and 57 components of SF. The major components of SF extract in the negative model were α, α-trehalose, DL-malic acid, D- (−)-fructose and xanthohumol, while in the positive model were oxymatrine, formononetin, (−)-maackiain and xanthohumol. Interestingly, oxymatrine and xanthohumol could significantly inhibit GBS infection in tilapia. Taken together, these results suggest that SF can inhibit GBS infection in tilapia, and it has potential for the development of anti-GBS agents.
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JOURNAL OF CHROMATOGRAPHY A
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2019
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IF 3.858
Wei Zhou,Yikun Liu,Xiaoyan Liao,Zilin Chen
Nonaqueous capillary electrophoresis (CE) and organic-aqueous CE using high content of organic solvents are common techniques in capillary electrophoresis-mass spectrometry (CE-MS) due to various advantages. However, the long-term stability of commonly used polyimide coated fused silica capillary remains a major problem that polyimide coating is prone to swelling in organic solvents and aminolysis in alkaline buffer, which cause serious problems like irregular electrospray, current drop or clogging event. In this work, robust PEEK capillary was used as separation capillary for tolerance to high content of organic solvents in CE-MS system. It is interesting that the high mechanical and chemical stability of PEEK capillary ensured stable CE separation and electrospray when used in CE-MS. All quantitative experiments were accomplished by single PEEK capillary. In addition, the using of organic solvents as running buffer reduced adsorption of hydrophobic compounds on PEEK capillary wall and improved the symmetry of peak shape. The PEEK-based CE-MS method showed good separation performance in analysis of several groups of active alkaloids in traditional Chinese medicines and was applied for quantitative analysis of peimine and peiminine in Bulbus Fritillariae thunbergii herb with high sensitivity. Based on the advantages like simple pretreatment, easy to cut, stable and good separation performance of PEEK column in CE-MS, this study provides an effective way for improving the stability and employment of high content of organic solvents in CE-MS analysis.
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JOURNAL OF CHROMATOGRAPHY A
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2018
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IF 3.716
Yu-Mei Zhao,Lv-Huan Wang,Si-Fan Luo,Qi-Qin Wang,Ruin Moaddel,Ting-Ting Zhang,Zheng-Jin Jiang
Neuraminidase (NA) is a glycoside hydrolase that has been proposed as a potential therapeutic target for influenza. Thus, the identification of compounds that modulate NA activity could be of great therapeutic importance. The aim of this study is to develop a drug discovery tool for the identification of novel modulators of NA from both compound libraries and natural plant extracts. NA was immobilized onto the surface of magnetic beads and the inherent catalytic activity of NA-functionalized magnetic beads was characterized. Based on the enzymatic activity (hydrolysis ratio), the inhibitory activities of 12 compounds from plant secondary metabolites were screened, and the desired anti-NA activities of flavonoids were certified. Ligand fishing with the immobilized enzyme was optimized using an artificial test mixture consisting of oseltamivir, lycorine and matrine prior to carrying out the proof-of-concept experiment with the crude extract of Flos Lonicerae . The combination of ligand fishing and HPLC-MS/MS identified luteolin-7- O -β-D-glucoside, luteolin, 3,5-di- O -caffeoylquinic acid and 3,4-di- O -caffeoylquinic acid as neuraminidase inhibitory ligands in Flos Lonicerae . This is the first report on the use of neuraminidase functionalized magnetic beads for the identification of active ligands from a botanical matrix, and it sets the basis for the de novo identification of NA modulators from complex biological mixtures.
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DESALINATION
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2025
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IF 9.8
Huifang Li,Yu Yue,Xiao Wang,Dahuan Liu
Solvent extraction has emerged as an extremely effective and highly promising approach for extracting lithium ions from salt lake brine, primarily due to its remarkable Li + selectivity, cost-effectiveness, and high efficiency. In this paper, the utilization of bis-(2,4,4-trimethylpentyl)-phosphinic acid (Cyanex 272) as an iron-fixed reagent in an organic solution, along with tributyl phosphate (TBP) as the extractant and FeCl 3 as the co-extractant, is proposed. Compared to the traditional TBP-FeCl 3 system, TBP-Cyanex 272 extraction system holds potential advantages in terms of a more environmentally friendly extraction process. Particularly, during the stripping process, the coordination of Fe 3+ with Cyanex 272 and TBP at a lower Cl − concentration allows Fe 3+ to be retained in the organic phase, thereby facilitating a greener extraction process. This method has effectively overcome the technical challenges associated with TBP-FeCl 3 systems, such as strong acid stripping and strong base saponification. Additionally, as an environmentally friendly extraction system, TBP-Cyanex 272 demonstrates exceptional performance in terms of lithium extraction efficiency and phase separation.
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ANALYTICAL LETTERS
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2013
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IF 0.965
Juan Zhang,Zilin Chen
A simple, rapid, and sensitive nonaqueous capillary electrophoresis-electrospray ionization-ion trap-mass spectrometry (NACE-ESI-IT-MS) method was developed for determination of matrine and oxymatrine in Sophora Flavescens and medicinal preparations. The conditions for NACE separation and MS detection were systematically optimized. The optimum NACE buffer contained 30 mM ammonium acetate, 1% acetic acid, and 15% acetonitrile in methanol and the applied voltage on separation capillary was set at 25 kV. Berberine was selected as internal standard. In order to generate a stable electrospray, a sheath liquid (isopropanol/H2O, 2/1, v/v) was used, which could also boost the flow through the ESI needle. The matrine and oxymatrine solutions were introduced into MS detection by a syringe pump for collecting the MSn spectra to investigate the main fragment ions and its possible cleavage pathways. Both matrine and oxymatrine showed good linearity in the concentration ranges from 0.5 to 400 µg/mL, with linear correlation coefficient R > 0.99 and the limit of detections were 37.5 ng/mL for matrine and 50.0 ng/mL for oxymatrine, respectively. The recoveries at different content of Sophora Flavescens were 98.3%–102.9% for MT and 95.3%–100.6% for OMT, which indicates the reliability of this method.
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LANGMUIR
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2024
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IF 3.7
Siyu Gan,Zhenxing Wang,Chunli Zheng,Zishen Lin,Ai-bin Zhu,Bo Lai
Commercial micron zerovalent iron (mZVI) and sulfur were used to prepare sulfidated micro zerovalent iron (S-mZVI) through ball milling. The corrosion potentials of mZVI and S-mZVI were −0.01 and −0.37 V, respectively, indicating S-mZVI possessed a stronger electron-donating ability. The practical antimony mine wastewater (C0(Sb(V)) = 3.8296 mg/L, pH = 8.29) was treated. If meeting the national discharge standard of 5 μg/L, 2.0 g/L mZVI and 1.6 g/L S-mZVI were required within 120 min. Passing N2 or reducing wastewater pH enhanced the treatment of Sb(V) by S-mZVI, in which the wastewater acidification was more effective. Once the wastewater pH was adjusted to 3.00, only 0.7 g/L S-mZVI and 40 min long time were needed to achieve the emission below 5 μg/L. Even S-mZVI underwent four cycles, and the final concentration of Sb(V) was as low as 4.67 μg/L. As the pHzpc value was 4.09 and the corrosion potential was −0.56 V at pH 3.0, the electron-donating ability of S-mZVI as well as the electrostatic attraction between the surface of S-mZVI and Sb(V) increased. Sulfidation of mZVI and then application under the acid condition significantly improved the treatment efficiency of Sb(V).
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JOURNAL OF MOLECULAR LIQUIDS
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2024
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IF 5.3
Fan Luo,Changchao Zhang,Yuyang Zhang,Jie Han,Ziteng Wang,Wenge Yang,Yonghong Hu
4-Hydroxyphenylacetamide (HPAD) is commonly used in the synthesis of high value-added chemicals in the fields of medicine, food and agriculture, such as atenolol, p -hydroxyphenylacetic acid, L -phenylalanine and D -phenylalanine. HPAD relies on chemical synthesis, involving multi-step reactions and multiple chemical catalysts. It is crucial to select appropriate organic solvent to improve yield and purity. In this work, the solubility of HPAD in 12 pure solvents (water, acetonitrile, methanol, ethanol, n-propanol, isopropanol, n-butanol, n-hexane, n-heptane, methyl acetate, ethyl acetate, n-butyl acetate) and 3 binary solvents (n-hexane + methanol, acetonitrile + methanol, n-heptane + methanol) was investigated in the range of 278.15–323.15 K. The solubility of HPAD in the selected solvents was positively correlated with the temperature. Hansen solubility parameters were used to study the dissolution behavior of HPAD. Then, the solvent effect was analyzed by KAT-LSER model, and it was found that the hydrogen bond acidity and nonspecific dipolarity/polarizability of solvents favored the dissolution of HPAD. Moreover, the modified Apelblat model (MA model), λ h model, CNIBS/R-K model (CNK model), Jouyban-Acree model (JA model), and SUN model were used to fit and analyze experimental data. The results showed that the MA model and the CNK model were the best models for single and binary solvents, respectively. Finally, van’t Hoff analysis showed that the dissolution of HPAD in the selected solvents was an endothermic and entropic driven process. This work provided reference data for the separation, purification and crystallization of HPAD, and had certain guiding significance for its industrial production and application.
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