FOOD CHEMISTRY
·
2020
·
IF 6.306
Yang Zhao,Ze-Hua Huang,Hui-Ming Zhou,Ke-Xue Zhu,Xiao-Na Guo,Wei Peng
Hexose oxidase was a feasible prevention for the dark spots in the fresh wet noodle sheets (FWNS). The chemical mechanism that hexose oxidase recucing the melanins of dark spots was discussed basis on the UPLC-TOF-MS analysis of the polyphenol oxidase (PPO)-catechol system. In the process of PPO browning, hexose oxidase catalyzed the oxidation of o -benzoquinone derivatives and their oligomers, hindering the formation of melanins. Hexose oxidase was efficient in FWNS with low ash content when water addition was 24%~44% or pH range was 4 ~ 7.5. Hexose oxidase could inhubit dark spots in the presence of 10 metal ions. The recommended addition amount was 40 ~ 60 ppm, by which the dark spots could be compolitely inhibited. Hexose oxidase was also suitable for wholewheat and oat FWNS, Δ L 6d of wholewheat and oat FWNS were reduced by 4 and 7.98, respectively.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
·
IF 6.1
Bin Wu,Shiyu Wang,Yunjian Ma,Shuguang Yuan,Frank Hollmann,Yonghua Wang
Alcohol oxidases (AOxs) catalyze the aerobic oxidation of alcohols to the corresponding carbonyl products (aldehydes or ketones), producing only H2O2 as the byproduct. The majority of known AOxs, however, have a strong preference for small, primary alcohols, limiting their broad applicability, e.g., in the food industry. To broaden the product scope of AOxs, we performed structure-guided enzyme engineering of a methanol oxidase from Phanerochaete chrysosporium (PcAOx). The substrate preference was extended from methanol to a broad range of benzylic alcohols by modifying the substrate binding pocket. A mutant (PcAOx-EFMH) with four substitutions exhibited improved catalytic activity toward benzyl alcohols with increased conversion and kcat toward the benzyl alcohol from 11.3 to 88.9% and from 0.5 to 2.6 s–1, respectively. The molecular basis for the change of substrate selectivity was analyzed by molecular simulation.
研究领域:
生物化学
·
酶学
·
食品科学
✓ 已验证
DOI
PubMed
高影响因子
PHYTOCHEMISTRY
·
2024
·
IF 3.2
Hao-Dong Yang,Yuan-Gui Yang,Zhi-Shu Tang,Kang Ma,Hong-Bo Xu
Acanthopanacis cortex (the dried root bark of Acanthopanax gracilistylus W. W. Smith) has been used for the treatment of rheumatic diseases in China for over 2000 years. Four previously undescribed lignans ( 1 – 4 ) and 12 known lignans ( 5 – 16 ) were isolated from Acanthopanacis cortex. In this study, the inhibitory activities of compounds 1 – 16 against neutrophil elastase (NE), cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) are reported. The results show that compounds 1 – 16 exhibit weak inhibitory activities against NE and COX-1. However, compounds 2 , 6 – 8 and 13 – 16 demonstrate better COX-2 inhibitory effects with IC 50 values from 0.75 to 8.17 μ Μ. These findings provide useful information for the search for natural selective COX-2 inhibitors.
研究领域:
药理学
·
中药学
·
天然产物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2018
·
IF 8.173
Xue Hu,Xidong Liu,Xiaodan Zhang,Hongxiang Chai,Yuming Huang
The fluorescent CuNCs/ZIF-8 nanocomposites were facilely prepared by mixing the PEI protected CuNCs with the precursors of ZIF-8, and characterized by UV–vis absorption, fluorescence, FT-IR, TEM , XRD and XPS . The quantum yield of the CuNCs/ZIF-8 is 15 times that of the PEI-CuNCs. Furthermore, the CuNCs/ZIF-8 possesses better stability and higher fluorescence response due to protective and confinement effects of MOFs . It was found that H 2 O 2 could cause much more quenching of fluorescent CuNCs/ZIF-8 than that of PEI-CuNCs, which might result from enriching H 2 O 2 by ZIF-8. The CuNCs/ZIF-8 can be designed as a fluorescence probe to selectively and sensitively detect H 2 O 2 with a linear range from 0.01 to 1.5 µM and a LOD of 0.01 µM, while those with PEI-CuNCs are 0.5–30 µM and 0.50 µM, respectively. Through formation of CuNCs/ZIF-8 hybrid, the sensitivity for the detection of H 2 O 2 increases by nearly 50-fold, which makes CuNCs/ZIF-8 a desirable probe to detect H 2 O 2 content in human serum samples. Also, we successfully demonstrated the potential application of the CuNCs/ZIF-8 for screening and evaluating activities of oxidase using glucose oxidase as a model. The glucose oxidase activity can be detected in a range of 0.1–10 mU/L with a LOD of 0.1 mU/L.
研究领域:
生物化学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
SCIENTIA HORTICULTURAE
·
2024
·
IF 3.9
Xiaojie Pei,Shuxin Sun,Xin Dai,Ke Liu,Ying Yang,Laifeng Lu,Yansheng Wang
Goji berry ( Lycium barbarum ) is a typical berry with a tender and thin pericarp, high moisture content and hollow interior, making it highly susceptible to mechanical damage caused by harvest and transportation. As a consequence, this susceptibility results in considerable economic losses within the fresh produce industry due to the degradation of fruit quality and subsequent decay. Here, oligogalacturonides (OGs), a well-characterized plant damage-associated molecular pattern, were applied to reduce postharvest loss and extend the shelf life of berries of the goji cultivar NingQi1. The loss rate in the OGs treatment was 44.45 % lower than that in the 0.8 mm compression treatments on Day 6, and the OGs treatment also effectively inhibited softening and delayed weight loss. The activity of superoxide dismutase increased by 27.4 % on the 4th day after OGs treatment, accompanied by a decrease in malondialdehyde content ranging from 14.2 % to 24.8 % on days 2 to 4 compared to the control group. Furthermore, the phenylalanine ammonia-lyase activity of OGs-treated goji berries was 57.0 % higher than that of the control at 6th days, which slowed the reduction in secondary metabolites such as total flavonoids. The findings of this study indicated that OGs may reduce postharvest losses caused by mechanical damage, extend the shelf life of goji berry by reducing softening and weight loss, and reduce wounding injury by minimizing free radical form of reactive oxygen species.
研究领域:
植物学
·
食品科学
·
园艺学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Tong Xing,Yaxin Lv,Gongqing Wu,Zhou Zhang,Wanqing Zhang,Xinping Wang,Zhuolang Chen,Weining Zhao,Felipe Conzuelo,Fangyuan Zhao
A new biofuel cell (BFC) was designed by assemblying a galactose oxidase (GalOx)-based bioanode and a bilirubin oxidase (BOD)-based biocathode. For the bioanode, GalOx was integrated within a poly(1-vinylimidazole-co-allylamine)-[Os(bispyridine) 2 Cl] redox polymer hydrogel nanostructured with multi-walled carbon nanotubes (MWCNTs), generating an electrocatalytic current density exceeding 1 mA cm −2 in the presence of 1 M glycerol. The biocathode was formed by immobilizing BOD on carbon cloth grafted with 4-aminobenzoic acid, showing efficient oxygen reduction reaction (ORR). When used as a gas diffusion electrode (GDE), the biocathode demonstrated improved ORR due to enhanced oxygen diffusion. The biocathode functioning as a GDE allows the bioanode working under oxygen-free conditions, enabling the construction of a membraneless BFC, generating a high power output. Crucially, the BFC operates with glycerol as the fuel, generating electricity while simultaneously converting glycerol into glyceraldehyde as a product with added value. In contrast with previous strategies requiring an energy input for the electrochemical oxidation of glycerol, converting an energy-consuming process into an energy-generating one. The membraneless BFC achieved a power density of (64 ± 3) μW cm −2 and an open circuit voltage (OCV) of (0.45 ± 0.02) V. When the system was operated in a two-compartment configuration, the power density increased to (143 ± 23) μW cm −2 , as both the anode and cathode could be operated under their respective optimal conditions. This work presents a novel BFC device, offering a sustainable and environmentally friendly method for producing both electricity and value-added chemicals by combining chemical, electrochemical, and enzymatic catalysis.
研究领域:
生物化学
·
能源科学
·
酶工程
✓ 已验证
产品关联
DOI
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Yujing Zhou,Jue Xu,Xiankang Fan,Zihang Shi,Wei Wang,Ming Huang,Kaiyong Yao,Hongbing Yan,Changyu Zhou,Yangyang Hu,Daodong Pan
This research sought to find a novel way to control the biogenic amines (BAs) in air-dried goose through pulsed electric field (PEF) combined with mixed bacteria fermentation. The findings indicated that Lactiplantibacillus plantarum ( L . plantarum ) PDD-1 and Lactococcus lactis subsp. lactis ( L . lactis ) JCM5805 exhibited superior monoamine oxidase (MAO) and diamine oxidase (DAO) activities and robust degradation abilities for the four BAs (putrescine, cadaverine, histamine, tyramine) across various environmental conditions (temperature, NaCl concentration, and initial pH). Subsequent investigations revealed that the mixed bacteria ( L . plantarum PDD-1: L . lactis JCM5805 at a 1:1 ratio) demonstrated a more pronounced effect on BAs reduction compared to individual starter culture, particularly for putrescine and tyramine. The study results indicated that the optimal conditions for reducing BAs in air-dried goose was an electric field strength of 0.7 kV/cm applied for 150 s, followed by air-drying for 4 d. Consequently, the total BA content in the air-dried goose decreased from 15.98 ± 1.27 mg/100 g to 7.54 ± 0.04 mg/100 g. Targeted amino acid metabolomics further defined the degradation mechanism. This study is instructive for low BAs meat processing methods.
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Jie-Qiong Chen,Shi-Fan Xue,Zi-Han Chen,Shengqiang Zhang,Guoyue Shi,Min Zhang
A novel label-free ratiometric fluorescent probe is developed for the detection of H 2 O 2 based on GelRed/[G 3 T] 5 /Tb 3+ hybrid, in which GelRed (a nucleic acid dye) intercalated into the designed single-stranded DNA [G 3 T] 5 (i.e. GelRed/[G 3 T] 5 ) acts as a stable build-in reference with red emission and Tb 3+ sensitized by [G 3 T] 5 (i.e. [G 3 T] 5 /Tb 3+ ) as a sensitive response signal with green fluorescence. With the successive addition of Hg 2+ and cysteine (Cys) to GelRed/[G 3 T] 5 /Tb 3+ , the fluorescence of [G 3 T] 5 /Tb 3+ can be effectively quenched and recovered respectively, while the fluorescence of GelRed/[G 3 T] 5 remains unchangeable. Combined with these properties, we have demonstrated its application for label-free ratiometric fluorescence detection of H 2 O 2 with self-calibration. The sensing mechanism is based on the specific reaction between H 2 O 2 and Cys, the resulting disulfide reverses the Cys-mediated fluorescence changes of [G 3 T] 5 /Tb 3+ . This method is further applied to the monitoring of oxidase-related reactions. As the glucose oxidase (GOx)-biocatalyzed oxidation of glucose and the acetylcholine esterase/choline oxidase (AChE/ChOx) cascade yield H 2 O 2 , such two biocatalytic processes are successfully examined utilized our proposed method, indicating its potential broad applications in biomedical analysis. In addition, we have also demonstrated its feasibility for oxidase-based visual biosensing, in which only a handheld UV lamp is used. Importantly, coupled with our proposed visual biosensing, a realtime quantitative scanometric assays has been also demonstrated by using a smartphone with easy-to-access color-scanning APP as the detection platform. Compared to traditional methods, the proposed design is cost-effective, simple to prepare and easy-to-use without fluorescence labeling or chemical modification.
研究领域:
分析化学
·
生物医学工程
·
生物化学
·
传感器与执行器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2025
·
IF 9.6
Qiliner Feng,Chen Wang,Si Shi,Junyu Fan,Yurong Chen,Tuanjie Zhang,Zhijun Ruan,Yongzheng Ma,Zifu Li,Jie Liu,Baizhu Chen
Tumor cells reprogram the energy metabolism and re-shape the microenvironment to maintain their fast proliferation and metastasis, leading to immunosuppression. Inspired by the design of engineered living materials, in this study, the AND-gated living hydrogel for metabolic-regulation enhanced tumor immunotherapy is constructed. Bacteria are genetically rewired to express lactate oxidase or glucose oxidase as two inputs under the control of thermosensitive promoter and then encapsulated inside the NIR-light controlled hydrogel. Triggered by laser, the engineered living hydrogel weakened the glycolysis and improved the mitochondrial respiration of tumor cells. The regulation of energy metabolism potentiated the antitumor immune responses by stimulating T cells, polarizing tumor associated macrophages to M1 phenotype, inducing the immunogenic cell death and stimulating the cGAS/STING pathway. With “high” inputs of two enzymes, the engineered living hydrogel realized the enhanced tumor immunotherapy as the “high” output. An approach of living hydrogel for metabolic-regulation AND-gated tumor immunotherapy is established.
研究领域:
肿瘤学
·
免疫学
·
合成生物学
·
生物材料
·
分子医学
·
转化医学
·
纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Huanhuan Jin,Ziyuan Wang,Zhongji Pu,Haoran Yu,Yuhuan Chen,Xiaoshu Zhou,Lirong Yang,Shuyun Ju,Jianping Wu
Optically pure 1,2,3,4-tetrahydroquinolines (THQs) represent a class of important motifs in many natural products and pharmaceutical agents. While recent advances on redox biocatalysis have demonstrated the great potential of amine oxidases , all the transformations focused on 2-substituted THQs. The corresponding biocatalytic method for the preparation of chiral 4-substituted THQs is still challenging due to the poor activity and stereoselectivity of the available enzyme . Herein, we developed a biocatalytic kinetic resolution approach for enantiodivergent synthesis of 4-phenyl- or alkyl-substituted THQs. Through structure-guided protein engineering of cyclohexylamine oxidase derived from Brevibacterium oxidans IH-35 A (CHAO), the variant of CHAO (Y215H/Y214S) displayed improved specific activity toward model substrate 4-phenyl substituted THQ (0.14 U/mg, 13-fold higher than wild-type CHAO) with superior ( R )-stereoselectivity ( E > 200). Molecular dynamics simulations show that CHAO Y215H/Y214S allows a suitable substrate positioning in the expanded binding pocket to be facilely accessed, enabling enhanced activity and stereoselectivity . Furthermore, a series of 4-alkyl-substituted THQs can be transformed by CHAO Y215H/Y214S, affording R -isomers with good yields (up to 50 %) and excellent enantioselectivity (up to ee > 99 %). Interestingly, the monoamine oxidase from Pseudomonas fluorescens Pf0–1 ( Pf MAO1) with opposite enantioselectivity was also mined. Together, this system enriches the kinetic resolution methods for the synthesis of chiral THQs.
研究领域:
生物催化
·
有机化学
·
蛋白质工程
·
药物科学
✓ 已验证
DOI
PubMed
高影响因子
Food and Bioprocess Technology
·
2016
·Vol. 9
·期 8
·1327-1334
·
IF 2.574
Ding Yang,Zhu Zhen,Zhao Jinhong,Nie Ying,Zhang Yu,Sheng Jiping,Meng Demei,Mao Hongmei,Tang Xuanming
Postharvest browning is the primary cause of a decrease in the shelf life of the white button mushroom ( Agaricus bisporus ). This study investigated the effect of postharvest brassinolide (BL) treatment on metabolism in relation to browning of the white button mushroom. Each harvested mushroom was dipped into one of three solutions containing 0, 1, or 3 μM BL for 5 min and stored in darkness at 4 °C for 16 days. Our results indicated that treatment with BL restrains browning development and reduces the total phenolic content and polyphenol oxidase activity. In addition, BL treatment maintains lower weight loss, electrolyte leakage, and malondialdehyde content and inhibits any increase in lipoxygenase activity compared with those of the control mushrooms. Furthermore, BL treatment significantly decreases the accumulation of reactive oxygen species (ROS) and induces the antioxidant enzyme system. Compared with 1 μM BL, treatment with 3 μM BL is more effective in reducing cap browning. The reduction of membrane oxidative damage and ROS levels induced by BL inhibits enzymatic browning reaction in the white button mushroom. These findings suggest that treatment with BL could have the potential of inhibiting browning and thus maintaining the mushroom’s commercial value.
研究领域:
农业
·
食品科学
·
植物生理学
·
采后技术
✓ 已验证
DOI
高影响因子
Frontiers in Pharmacology
·
2022
·
IF 5.988
Gao Tianshu,Xu Jin,Xiao Yuxiao,Li Jiaqi,Hu Weifeng,Su Xiaoyu,Shen Xudong,Yu Wan,Chen Zhen,Huang Baosheng,Li Honglei,Wang Xing
Objective: To observe the antioxidative effects of N-(9,10-anthraquinone-2-ylcarbonyl) xanthine oxidase inhibitors (NAY) in vitro and in vivo models of hyperuricemia and explore the mechanism.Methods: A classical experimental method of acute toxicity and a chronic toxicity test were used to compare the toxic effects of different doses of NAY in mice. The hyperuricemia mouse model was established by gavage of potassium oxonate in vivo. After treatment with different doses of NAY (low dose: 10 mg/kg, medium dose: 20 mg/kg, and high dose: 40 mg/kg) and allopurinol (positive drug, 10 mg/kg), observe the levels of uric acid (UA), creatinine (CRE), and urea nitrogen (BUN) in urine and serum, respectively, and detect the activities of xanthine oxidase in the liver. The hyperuricemia cell model was induced by adenosine and xanthine oxidase in vitro. The cells were given different doses of NAY (50, 100, and 200 μmol/L) and allopurinol (100 μmol/L). Then the culture supernatant UA level of the medium was measured. The next step was to detect the xanthine oxidase activity in the liver and AML12 cells, and the levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1β), and NOD-like receptor thermal protein domain-associated protein 3 (NLRP3) inflammatory factors in the kidney and serum of mice. Western blot was used to detect xanthine oxidase protein expression in mouse liver tissue and AML12 cells, ASC, Caspase-1, NLRP3, GLUT9, OAT1, and OAT3 protein expression in mouse kidney tissue and HK-2 cells. Hematoxylin–eosin staining was used to stain the liver and kidney tissues of mice and observe the tissue lesions.Results: NAY had little effect on blood routine and biochemical indexes of mice, but significantly reduced the serum UA level. NAY significantly reduced the level of UA in hyperuricemia mice and cells by inhibiting xanthine oxidase activity and reduced the levels of TNF-α, IL-6, and other inflammatory factors in serum and kidney of mice. NAY can inhibit inflammation by inhibiting the NLRP3 pathway. In addition, NAY can downregulate GLUT9 protein expression and upregulate OAT1 and OAT3 protein expression to reduce the UA level by promoting UA excretion and inhibiting UA reabsorption.Conclusion: These findings suggested that NAY produced dual hypouricemic actions. On the one hand, it can inhibit the formation of UA by inhibiting xanthine oxidase inhibitors activity, and on the other hand, it can promote the excretion of UA by regulating the UA transporter. It provides new ideas for the development of hyperuricemia drugs in the future.
研究领域:
药理学
·
生物化学
·
分子生物学
·
炎症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）