BIOSENSORS & BIOELECTRONICS
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2023
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IF 12.6
Yuxin Li,Ping Li,Yiqing Chen,Yue Wu,Jing Wei
The low polarization and low Raman cross section characteristics of glutathione (GSH) make it challenging to directly detect GSH molecules through surface enhanced Raman scattering (SERS) technology. Development of nanostructures for indirect detection of GSH applied to the SERS platform is of great interest. Herein, silver nanoparticles (Ag NPs)/copper-polyphenol colloidal spheres (denoted as CuTA@Ag) with adjustable Ag NPs coverage are prepared by deposition of Ag NPs on the metal-polyphenol colloidal spheres via an interfacial polyphenol reduction method. The size and density of the Ag NPs deposited on the out layer can be readily adjusted by tailoring the concentrations of silver precursor. It leads to activity difference for the nanozyme and SERS characteristics. The SERS properties of the obtained CuTA@Ag are studied using oxTMB , catalytic products of nanozyme, as the probing molecules. They provide satisfactory SERS performance with a low detection limit of 10 −7 M (S/N = 3) and linear determination in the 1–100 μM range for GSH. Moreover, it is further able to detect the glutathione content in cancer cells with well accurate and reproducible capability, catching the signs of rising cancer marker levels. This work proposes structurally tunable nanomaterials platform for a catalytic-based SERS assay, which is expected to utilize the high sensitivity of SERS tool for GSH detection in the cellular environment.
研究领域:
纳米技术
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分析化学
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生物传感器
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癌症研究
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DOI
PubMed
高影响因子
Food Analytical Methods
·
2023
·Vol. 16
·期 9
·1477-1486
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IF 2.9
Liu Lei,Zhao Binyu,Luo Xiaogang,Meng Lingkui,Wu Fengshou
Porous organic polymers (POPs) are a class of materials that combine the properties of both polymers and porosity. They are characterized by their network structure composed of repeating organic units, which form pores or cavities throughout the material. Owing to their unique features including tunable porosity, abundant accessible active sites and synthetically control ability, POPs have been widely employed in the fields of catalysis, adsorption, and biomedicine. The incorporation of porphyrin building units into POPs endows POPs with unique structural characteristics and excellent optical properties. In this work, the aldehyde porphyrin with polyethylene glycol chain was successfully synthesized with good hydrophilicity, which was then reacted with tetrakis(4-aminophenyl) methane to construct the porphyrin-based porous organic polymers. The porphyrin-based POPs was then metallized to yield iron-containing porous organic polymers (Fe-POPs), which could catalyze the transformation of colorless substrate (3,3′,5,5′-tetramethylbenzidine (TMB)) to a blue product (ox-TMB) in the presence of hydrogen peroxide (H 2 O 2 ). Upon the addition of the ascorbic acid (AA) to the aforementioned system, the blue solution faded gradually to colorless. Based on this phenomenon, a rapid and sensitive colorimetric assay for the measurement of H 2 O 2 and AA was developed using Fe-POPs as mimetic enzyme. Fe-POPs was then successfully applied in real samples to detect AA with high accuracy. As a potential peroxidase mimic, Fe-POPs demonstrated the benefits of excellent stability and catalytic efficiency, which presented the promising application in the area of biocatalysis and biosensing. Graphical
研究领域:
材料科学
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化学生物学
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分析化学
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生物化学
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纳米技术
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
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2022
·
IF 6.911
Shengmei Tai,Zhijuan Qian,Hongxin Ren,Alberta Osei Barimah,Chifang Peng,Xinlin Wei
A colorimetric assay based on an enzyme-inhibition strategy is promising for the on-site detection of pesticide residues . However, very few works of pesticide detection were reported based on the inhibition toward nanozymes although nanozymes have demonstrated many advantages in sensing various targets. Herein, a facile colorimetric detection for Glyp was developed based on β [email protected] enzyme mimics. The β [email protected] with high peroxidase-like activity was synthesized using random DNA double strands as template and β -CD as surface ligand. β [email protected] could catalyze the H 2 O 2 -3,3′,5,5′-tetramethylbenzidine (TMB) system. The oxidation product OxTMB with a blue color and presented a large absorption signal at 652 nm. However, Glyp could destroy the synergic effect between redox doublet (Cu 2+ /Cu + ) on the β [email protected] A-CuNCs surface, resulting in the inhibition of the peroxidase-like activity. Colorimetric detection for Glyp could be established by detecting the changes of absorption signal at 652 nm. The linear range was 0.02–2 μ g/mL and the detection limit was 0.85 ng/mL (3δ/s). The method was applied in measuring Glyp spiked in lake water and various food samples. This method had rapidness, high sensitivity, and selectivity advantages, indicating the high application potential in monitoring Glyp residue in food.
研究领域:
分析化学
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环境科学
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食品安全
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农业化学
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DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2019
·Vol. 411
·期 18
·4063-4071
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IF 3.286
Lin Shan,Zheng Danmin,Li Ailing,Chi Yuwu
It is well known that 3,3′,5,5′-tetramethylbenzidine (TMB) can be oxidized into blue or yellow oxidzed TMB (oxTMB) with the catalysis of peroxidase or mimetic enzyme of platinum nanoparticles (Pt NPs). In this work, we found that TMB could be oxidized into very stable black oxTMB with the catalysis of Pt NPs under certain chromogenic reaction conditions. For the first time, the black oxTMB was revealed to consist of nanowires (oxTMB NWs) with lengths of more than 100 μm and diameters of around 100 nm. The black oxTMB NWs showed very strong light absorption ability, thus could be used to greatly amplify the signal of Pt NP-based immunochromatography test strips (ICTSs). The Pt NP-based ICTSs with black oxTMB NW signal amplification have shown much better assay ability (linear response range and limit of detection) than those of gold nanoparticle (Au NP)-based ICTS, Pt NP-based ICTS, and Pt NP-based ICTS with blue or yellow oxTMB signal amplifications. The developed Pt NP-oxTMB NW-based ICTS has been demonstrated to be a new, accurate, sensitive, selective, and rapid immunosensor for quantitative detection of antigens such as human chorionic gonadotropin (HCG).
研究领域:
分析化学
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纳米技术
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生物传感器
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）