JOURNAL OF ETHNOPHARMACOLOGY
·
2025
·
IF 5.4
Yan Du,Yu-lang Chen,Ying Zhang,Yan-lin Zhao,Zhong Huang,Peng Jin,Shuai Ji,Dao-Quan Tang
Ethnopharmacological relevance Mulberry ( Morus alba L.) leaf is a well-known herbal medicine in China for thousands of years. Mulberry leaf can regulate arachidonic acid (ARA) metabolism disorder in obesity and type 2 diabetes mellitus. However, the active ingredients involved in this process are still unclear. Aim of study To explore the potential active ingredients in mulberry leaf against ARA metabolism disorder. Materials and methods In this research, an efficient method combining affinity ultrafiltration, molecular docking, and network pharmacology was developed and applied to explore cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX) inhibitors from mulberry leaf. Results A total 17 potential inhibitors were screened by affinity ultrafiltration assay and identified by high resolution mass spectrometry. In addition, 8 bioactive ingredients were obtained after re-evaluated by molecular docking and network pharmacology, and their inhibitory activities on COX-2 and 5-LOX were confirmed by in vitro inhibitory assays. The results of cell experiments showed that the expressions of COX-2 and 5-LOX were significantly suppressed by neochlorogenic acid, rutin, isoquercetin, and (−)-syringaresinol-4-O-glucoside. Conclusion Neochlorogenic acid, rutin, isoquercetin, and (−)-syringaresinol-4-O-glucoside may be the potential material basis of mulberry leaf in the regulation of ARA metabolism of obesity and type 2 diabetes mellitus.
研究领域:
药理学
·
分析化学
·
中药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2025
·
IF 9
Bingyang Lu,Junchang Guo,Hao Yang,Xiao Han,Peng Yu,Shengjun Cheng,Zhenxu Shi,Dehui Wang,Weifeng Zhao,Jing Luo,Xu Deng
Gas exchange membranes, as the crucial components of extracorporeal membrane oxygenation (ECMO) systems, enable efficient O 2 /CO 2 exchange in blood while maintaining adequate oxygen saturation in patients. However, clinical gas exchange membranes remain prone to thrombosis complications, which severely compromise their gas exchange effectiveness. To address this, we propose a blood-repellent gas exchange membrane (BRGEM) that helps in preventing thrombosis due to its minimal solid-liquid contact areas, while significantly enhancing gas exchange through a gas-liquid direct contact diffusion strategy. Notably, the BRGEM exhibits a 22.09-fold reduction in thrombus formation without anticoagulants compared to commercial ECMO membranes, demonstrating its exceptional anti-fouling properties. Furthermore, the membrane enables rapid oxygen recovery in the acute respiratory distress syndrome rabbit model, achieving approximately 12.97-fold improvement. We anticipate this strategy will significantly advance the development of next-generation blood oxygenation membranes and artificial lung technologies, offering transformative potential for future clinical applications.
研究领域:
生物医学工程
·
膜技术
·
呼吸医学
·
生物材料
·
人工器官
✓ 已验证
产品关联
DOI
高影响因子
ENERGY & FUELS
·
2022
·
IF 4.654
Yang Xu,Yudi Zhao,Xuan Guo,Yunming Fang
Co-processing of bio-oil and petroleum-derived feed in a fluid catalytic cracking process of a refinery is considered to be one of the most potential bio-liquid fuel preparation technologies. The detailed qualitative and quantitative analysis of the oxygenates in the co-cracking products facilitates optimization of the subsequent processing. In this study, an in-depth characterization of trace oxygenates from home-made co-processing liquid products in a fluid catalytic cracking (FCC) pilot plant unit at different bio-oil water-soluble phase (BOWS)/vacuum gas oil (VGO) mass blending ratios (0/100, 10/90, 20/80) at different reaction temperature (500 and 525 °C) was performed with quantitative and qualitative comprehensive two-dimensional gas chromatography–mass spectrometry (GC × GC–MS). An internal standard method with the internal standard 1,1,3,3-tetramethoxypropane was used to quantify trace oxygenates, which was selected from the qualitative analysis of the co-processing liquid products. The results showed that the contents of phenols and benzofurans in the liquid products from co-processing were low and the addition of BOWS was beneficial to improve the co-processing conversion, reduce the coke yield, but reduce the gas products and gasoline yield. The deoxygenation of catalytic cracking for oxygenates also weakened with the increase of bio-oil addition. The increase of reaction temperature can strengthen the deoxygenation of catalytic cracking, but the excessively high temperature can strengthen the thermal cracking reaction and reduce the gasoline yield.
研究领域:
能源化学
·
分析化学
✓ 已验证
DOI
高影响因子
FOOD SCIENCE AND BIOTECHNOLOGY
·
2015
·Vol. 24
·期 4
·1257-1263
·
IF 0.653
Huang Yechuan,Wu Zhaomin,Wang Yanrong,Li Feng
To investigate the effects of pressure and temperature on lipoxygenase (LOX) in meat, changes in LOX activities in minced pork and in crude enzyme extracts under different conditions of pressure (131.82–468.18 MPa), temperature (23.18–56.82°C), and duration (6.59–23.41 min) were evaluated using response surface methodology. LOX activities in extracts exhibited a decrease with an increasing treatment temperature, pressure, or duration. Each of these factors alone had a significant ( p <0.05) effect on LOX activities. Activation of LOX in most minced pork treated at less than 400 MPa was observed. Treatment pressure and temperature, and interactions between pressure and temperature and between duration and pressure, significantly ( p <0.05) affected LOX activities. LOX showed higher stability in muscles than in extracts during combined pressure-heat treatments. The most important factor affecting LOX was treatment temperature for extracts and treatment pressure for muscles.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
高影响因子
Foods
·
2022
·Vol. 11
·期 7
·980
·
IF 5.561
Jiamei Xu,Yu Liu,Jingjing Ma,Pengpeng Li,Zhiming Geng,Daoying Wang,Muhan Zhang,Weimin Xu
Lipoxygenase (LOX)is a major endogenous enzyme for the enzymatic oxidation of lipids during meat storage and meat product manufacturing. In the present work, some characteristics, i.e., effects of inhibitors, selectivity of substrates and specificity of oxidation products, were studied using recombinant porcine 12-lipoxygenase catalytic domain(12-LOXcd). Several familiar inhibitors were found inhibit the activity of recombinant porcine 12-LOXcd;nordihydroguaiaretic acid demonstrated the strongest inhibitory effect. The enzyme could oxygenate common polyunsaturated fatty acids, and showed the highest affinity to linoleic acid (LA), followed by arachidonic acid (AA), linolenic acid (LN) and docosahexaenoic acid (DHA). Under the action of porcine 12-LOXcd, LA was oxidized into four hydroxyoctadecadienoic acid (HODE) isomers, i.e., 13-Z,E-HODE, 13-E,E-HODE, 9-Z,E-HODE and 9-E,E-HODE. Variation of pH not only affected the yield of LA oxidation products, but also the distribution of HODE isomers. These results indicated that endogenous LOX activity and LOX-catalyzed lipid oxidation can be regulated during meat storage and meat product manufacturing.Keywords:Lipoxygenase;inhibitors;substrates;oxidation products;linoleic acid;porcine
研究领域:
食品科学
·
生物化学
·
酶学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Zihao Yin,Wanqing Wei,Wei Song,Jian Wen,Guipeng Hu,Xiaomin Li,Cong Gao,Jia Liu,Jing Wu
Epoxides, a class of ethers with a three-membered ring structure, are widely used in the textile, pharmaceutical, and packaging industries. Chemoenzymatic epoxidation presents a promising method for synthesizing epoxides. However, its epoxidation efficiency is hindered by low chemoselective perhydrolysis, which is caused by the hydrolysis side reaction in the aqueous phase. In this study, a chemoenzymatic epoxidation process in the aqueous phase was developed by utilizing an acyltransferase from P. litoralis (PlAcT) for its chemoselective perhydrolysis. Crystal structure analysis, molecular dynamics simulations, and quantum mechanics calculations, along with site-specific mutagenesis, revealed that the selectivity of perhydrolysis is due to a lower energy barrier in the acyl transfer step compared to that in hydrolysis. Furthermore, the mutant PlAcTM3–2 exhibited a 7.6-fold improvement in solvent stability and a 1.3-fold increase in perhydrolysis activity compared to the wild type, achieved by reshaping interface interactions. As a result, the engineered strain Y07, harboring PlAcTM3–2, successfully synthesized compounds 3a–3n with conversions ranging from 11–99%, and the titers of compounds α-pinene oxide(3i), β-pinene oxide(3j), 3-carene oxide(3k), and limonene dioxide(3l–3) reached 55.8, 16.7, 75.2, and 21.4 g/L, respectively. These results demonstrate a sustainable method for chemoenzymatic epoxidation in the aqueous phase.
研究领域:
生物化学
·
酶工程
·
绿色化学
✓ 已验证
DOI
PubMed
高影响因子
FOOD SCIENCE AND BIOTECHNOLOGY
·
2015
·Vol. 24
·期 3
·867-873
·
IF 0.653
Niu Liying,Li Dajing,Liu Chunquan,Liu Fuguo
Lipoxygenases (LOX) in milk-stage sweet corn and waxy corn were extracted using a phosphate buffer (pH 7.0) and enzyme activities were determined using linoleic acid as a substrate. Michaelis constant ( K m) values, decimal reduction times (D value), temperature sensitivity parameters (Z value), and activation energies ( E a) were calculated. Enzymes from both corn types followed first-order inactivation kinetics within 0–25 min and 50–70°C. However, enzymes exhibited different pH profiles and affinities toward linoleic acid. Km values (4.34 and 1.40 mM for sweet corn and waxy corn, respectively), heat stability values, and Ea values (116.81 and 246.82 kJ/mol) were different. Waxy corn LOX was more heat stable below 65–C with a higher D value, but was more temperature sensitive with a lower Z value. The different characteristics suggested the presence of different isoenzymes and necessitated the use of different parameters for blanching.
研究领域:
食品科学
·
植物生理学
✓ 已验证
产品关联
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2023
·
IF 8.1
Qiaoyu Liu,Menglin Lei,Jianjun Lin,Wenhong Zhao,Xiaofang Zeng,Weidong Bai
The roles of enzymatic (Lipoxygenases, LOX) oxidation and autoxidation in the dry-cured processing of mackerel were investigated by adding exogenous substances in this study. Four groups, namely control, chlorogenic acid (inhibiting LOX activity), EDTA-2Na (inhibiting autoxidation), and exogenous LOX (adding eLOX), were assigned. The results showed that lipid oxidation of mackerel was reduced by inhibiting LOX activity and autoxidation, while adding eLOX promoted lipid oxidation. Inhibition of LOX activity and autoxidation suppressed fatty acid accumulation mainly in the air-drying and curing stage, respectively. The total contents of key flavors in the mackerel during dry-cured processing were decreased by inhibiting LOX activity and autoxidation, and the former inhibitory effect was stronger than autoxidation, while it was corresponding increased through adding eLOX, of particular in the later stage of air-drying. Collectively, LOX could promote the flavor formation of the mackerel in the dry-cured processing, which could be applied in the flavor adjustment of aquatic products or some similar fields.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Ang Yang,Huibing Chi,Zhaoxin Lu,Fengxia Lu,Ping Zhu
Lipoxygenase (LOX) can catalyze the production of hydroperoxides from polyunsaturated fatty acids, offering promising applications in the food industries with especial contribution of naturally modified function for food gelation. To date, commercially available LOXs primarily sourced from soybean meal, facing challenges such as containing isoenzymes and exhibiting low catalytic activity for broad utilization restrictions. Herein, a LOX was first derived from the marine microorganism Aliikangiella coralliicola (AcLOX) with a molecular weight of 80 kDa expressed in Escherichia coli . AcLOX exhibited good stability over a wide pH range of 4.0–9.0. Additionally, AcLOX displayed specific activity against α-Linolenic acid (ALA) of 6.67 × 10 4 U/mg, and kinetic parameters values for K m , k cat , and k cat / K m of 0.58 μM, 1.40 s −1 , and 2.42 μM −1 s −1 , respectively. Moreover, Ca 2+ and dimethyl sulfoxide (DMSO) activated the activity of AcLOX. To explore its gelation modification ability, AcLOX was applied to induce the cross-linking of soy protein isolate (SPI) subunits with increased particle size, potential, surface hydrophobicity (S o ), and as well as gel hardness at appropriate concentrations. This is the inaugural study to unveil the gelation modification ability of LOX from Aliikangiella coralliicola . These findings provide superior enzymatic performance and cross-linking potential of AcLOX for industrial applications.
研究领域:
食品科学
·
酶学
·
生物化学
✓ 已验证
DOI
高影响因子
ULTRASONICS SONOCHEMISTRY
·
2022
·
IF 9.336
Ting-ting Chai,Yang-na Huang,Shao-tian Ren,Dan-li Jin,Jing-jing Fu,Jun-yan Guo,Yue-wen Chen
Lipid oxidation will lead to the deterioration of flavor, color and texture of aquatic products with high fatty acid content. The mechanism of ultrasound (US) combined with rosmarinic acid (RA) on lipid oxidation and endogenous enzyme activities of large yellow croaker during cold-storage (4 ℃) was investigated. The result showed that the US and RA have synergistic effects in delaying lipid oxidation and inhibiting endogenous lipase and lipoxygenase (LOX) activities related to oxidation. The inhibition of LOX activity by RA was dose-dependent, and US showed a negative effect on the inhibition of enzyme activity in the presence of low concentration RA. Moreover, RA changes the enzyme structure through static fluorescence quenching and interaction with enzyme molecules. Hydrogen bonding and hydrophobic interaction are the main interaction forces between RA and LOX. This study could provide basic mechanism of US treatment cooperating with polyphenols to inhibit lipid oxidation during food preservation.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2022
·
IF 10.53
Lunhao Zhi,Siyu Li,Xueqin He,Yunbo Feng,Chong Cheng,Shuang Li,Shudong Sun,Changsheng Zhao
Extracorporeal membrane oxygenation (ECMO) is one of the most important supporting therapies for patients with severe cardiopulmonary failure, and the core component of ECMO is the membrane oxygenator . However, in clinical application, the membrane oxygenator faces a dilemma in balancing anticoagulation and hemorrhage risks, which restrains the service life of membrane oxygenator and affects patients’ safety seriously. In this work, we introduce poly (1-vinyl-2-pyrrolidone) (PVP) and poly (acrylic acid) (PAA) into polyethersulfone (PES) membranes simply by in-situ crosslinking polymerization and nonsolvent induced phase separation (NIPS) method. All the modified membranes exhibit favorable hemocompatibility with prolonged activated partial thromboplastin time (APTT) (>10 s). Besides, the introduction of PAA can improve CO 2 and O 2 permeabilities simultaneously (increased by 36.57% and 30.86% at maximum, respectively, compared with pristine PES membrane). Finally, an ECMO-simulated gas-liquid contactor circulation device is designed, and the modified membranes show better oxygenation performance and CO 2 removal performance. The membrane modification and fabrication methods in this work are simple, low-cost, and easily industrialized. The work has practical guiding significance for the subsequent membrane research and application toward oxygenators.
研究领域:
生物医学工程
·
材料科学
·
医疗器械
✓ 已验证
产品关联
DOI
高影响因子
Foods
·
2023
·Vol. 12
·期 13
·2504
·
IF 5.2
Qiaoyu Liu,Menglin Lei,Wenhong Zhao,Xiangluan Li,Xiaofang Zeng,Weidong Bai
In this study, lipoxygenase (LOX) extracted from dry-cured mackerel was purified, resulting in a 4.1-fold purification factor with a specific activity of 493.60 U/min·g. LOX enzymatic properties were assessed, referring to its optimal storage time (1–2 days), temperature (30 °C), and pH value (7.0). The autoxidation and LOX-induced oxidation of palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:2n9c), linoleic acid (C18:2n6c), arachidonic acid (C20:4), EPA (C20:5), and DHA (C22:6n3) were simulated to explore the main metabolic pathways of key flavors in dry-cured mackerel. The results showed that the highest LOX activity was observed when arachidonic acid was used as a substrate. Aldehydes obtained from LOX-treated C18:1n9c and C18:2n6c oxidation, which are important precursors of flavors, were the most abundant. The key flavors in dry-cured mackerel were found in the oxidative products of C16:0, C18:0, C18:1n9c, C18:2n6c, and C20:4. Heptanaldehyde could be produced from autoxidation or LOX-induced oxidation of C18:0 and C18:1n9c, while nonal could be produced from C18:1n9c and C18:2n6c oxidation. Metabolic pathway analysis revealed that C18:1n9c, C18:2n6c, EPA, and DHA made great contributions to the overall flavor of dry-cured mackerel. This study may provide a relevant theoretical basis for the scientific control of the overall taste and flavor of dry-cured mackerel and further standardize its production.Keywords:lipoxygenase;fatty acids;autoxidation;enzymatic oxidation;volatile compounds
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）