Analytical Methods
·
2022
·Vol. 15
·期 4
·529-536
·
IF 3.532
Zian Wang,Zhiwei Liu,Tian Guan,Xi Zeng,Runlin Shen,Zhaodong Li,Yi Lei,Zhenlin Xu,Zhili Xiao,Hongtao Lei,Dingqiang Huang
With the popularity of herbal tea in China, many food fraudsters have added illegal drugs to herbal tea to enhance its functions, among which aminopyrine is widely abused as an antipyretic and analgesic. Presently, there is no immunoassays for aminopyrine, and it is difficult to achieve real-time detection in the field. Based on a polyclonal antibody of aminopyrine with high specificity and sensitivity, an optimal combination of coating antigen/antibody was obtained by screening different coating antigens. On this basis, a sensitive ic-ELISA method was established to detect aminopyrine in herbal tea. The detection limit of the ic-ELISA was 0.18 ng mL−1, which was much lower than the 100 ng mL−1 required as a standard. The method had good consistency with LC-MS in the detection of actual samples and could be used as a reliable method for the detection of aminopyrine in herbal tea.
研究领域:
食品科学
·
分析化学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2014
·Vol. 6
·期 15
·5925-5933
·
IF 1.938
Lei Zheng,Hui Mao,Liying Zhang,Yang Jin,Yujie Zhou,Yan Peng,Shuhu Du
The adulteration of traditional Chinese medicine (TCM) with synthetic drugs severely harms the TCM effect and public health. Thus, quick detection of synthetic drug found in TCM is crucial. Here, we report surface-enhanced Raman scattering (SERS) coupled with density functional theory (DFT) calculations for the direct identification and detection of aminopyrine (AP) in TCM using silver-coated gold nanoparticles (Au@Ag NPs). It has been demonstrated that the Raman enhancement of Au@Ag NPs to AP was stronger than that of Au, Ag and Ag@Au NPs, and there was a strong dependence of the Raman enhancement on the substrate concentration. Moreover, we obtained the DFT calculation result of AP and found that the calculation result was in good agreement with the experimental one. In addition, the characteristic peak at 999 cm−1, which was the strongest peak in the SERS spectra, was used for a quantitative evaluation of the AP level. The limit of detection (LOD) was 2.50 × 10−7 M (equal to 57.8 ng mL−1) in aqueous solution, which was in the same order of magnitude as that obtained by liquid chromatography-mass spectrometry (LC-MS), and the recovery of AP ranged from 93.9% to 101% in the spiked TCM (Shexiang Fengshi capsule) sample. Each test using the SERS sensor only needed a 10 μL sample solution and the whole test could be accomplished within ∼3 min. The SERS technique can be applied to authenticate and detect trace AP in real herbal products.
研究领域:
药学
·
分析化学
·
计算化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2020
·
IF 5.065
Yanqing Wang,Qianqian Han,Hongmei Zhang,Yuanyuan Yan
In this work, the binding interactions of norfloxacin (NRF) with p -acetylaminophenol (AAP), aspirin, ibuprofen , and aminopyrine were investigated by molecular modeling and fluorescence quenching methods. The results indicated the existence of the electrons transferring during the π–π stacking interaction with face to face stacking of them. NRF acts as electron acceptor during the NRF-AAP and NRF-aminopyrine complexes and acts as electron donor during the NRF-aspirin, NRF-ibuprofen complexes. Fluorescence quenching of NRF induced by AAP, aspirin, ibuprofen, and aminopyrine are the formation of complexes. The binding ability of NRF with AAP, aspirin, ibuprofen, and aminopyrine indicated that the order are from top to bottom aminopyrine, AAP, aspirin, and ibuprofen and the number of the binding site n is about 1.
研究领域:
药理学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2023
·
IF 3
Zhixuan Sun,Miao Li,Sixuan Qian,Yani Gu,Jingjie Huang,Jiancheng Li
The ultra-performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) detection method was developed for the residues of 10 NSAIDs (salicylic acid, acetylsalicylic acid, acetaminophen, diclofenac, tolfenamic acid, antipyrine, flunixin meglumine, aminophenazone, meloxicam, metamizole sodium) in swine muscle, liver, kidney, and fat. Swine tissue samples were extracted by phosphorylated acetonitrile with the addition of an appropriate amount of internal standard working solution, defatted with acetonitrile-saturated n -hexane, and purified by Hydrophile-Lipophile Balance (HLB) solid-phase extraction column, then separated by UPLC BEH shield RP 18 column with 0.1% formic acid in water/0.1% formic acid in acetonitrile with gradient elution, which was detected in the multiple reaction monitoring (MRM) modes. The correlation coefficient of the standard curve equation is greater than 0.99, and the coefficient of variation within and between batches is less than 14.4%. We evaluated the analytical method using two green assessment tools. The method established in this study met the requirements of NSAID residue analysis and provides analytical tools for determining and confirming NSAIDs in swine tissue samples. This is the first report on the simultaneous determination of 10 NSAIDs in four swine tissues by the UPLC-MS/MS method and accurate quantification using deuterated internal standards.
研究领域:
分析化学
·
兽医学
·
药理学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）