Pharmaceutics
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2026
·Vol. 18
·期 7
·859
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IF 6.9
Xin Shen,Mian Zhao,Muhammad Hamza,Wen-Ting Wu,Jing Liu,Zi-Lu Guo,Zhi-Yu Guan,Zhe Li,Wei-Feng Zhu
Background: As a novel drug delivery vehicle, transfersomes (TFSs) have great deformability, good biocompatibility, and biodegradability, which can significantly improve drug delivery efficiency. However, their application is limited by their poor stability and vulnerability to leaks.Methods: This paper prepared and assessed the stability of transfersomes co-loaded with Pueraria total flavonoids (PTFs) and tanshinone (TAN), known as PTF/TAN-TFSs, in order to overcome this constraint.Results: The findings showed that the co-loaded system (PTF/TAN-TFSs) had better structural stability than the single-loaded systems (PTF-TFSs and TAN-TFSs), with drug leakage rates decreased by roughly 5% and 7%, respectively, and a more uniform size distribution with a particle size of 44.52 ± 0.47 nm and a PDI of 0.22, while the single-loaded systems had PDI values larger than 0.3. The co-loaded system showed a better sustained-release profile than the suspension with a 15.28% decrease in the maximum cumulative release of the poorly soluble component Tan IIA. Additionally, the co-loaded system demonstrated enhanced solubilization capability, making the poorly soluble component TAN more soluble by 89.52 times compared to the free medication and by 1.97 times compared to the single-loaded system. Furthermore, the co-loaded system significantly improved the intestinal absorption of Tan IIA, increasing the Papp and Ka values in the ileum segment by 2.02-fold and 1.80-fold, respectively.Conclusions: The stability of the system was successfully improved by co-loading many components with varying hydrophobicities. This method provides a fresh way to enhance transferosome stability, broadening their application in the field of drug delivery.
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Frontiers in Pharmacology
·
2026
·
IF 5.4
Yihuang Liu,Xiaofeng Li,Xia Zhang,Hua Cao,Nailin Zhang,Jianhui Sun,Pingping Chen
Introduction This research sought to investigate the therapeutic efficacy and underlying mechanisms of Shengjiang Powder (SJP) in the context of chronic gastritis. Methods A rat model of gastric mucosal injury was induced by 1-methyl-3-nitroso-1-nitrosoguanidine (MNNG) and a 10% NaCl solution, followed by treatment with either Vitacoenzyme or SJP. Histopathological changes in gastric tissues were evaluated using hematoxylin-eosin staining. Serum IL-6 levels were measured by enzyme-linked immunosorbent assay. Western blot (WB) analysis was performed to detect COX-2 expression. Immunohistochemistry was used to assess TFF1 and TFF2 expression. Serum metabolomics was conducted to identify differential metabolites and enriched pathways, while proteins related to the FOXO signaling pathway were further examined in vivo . WB analysis was used to detect SIRT1, FOXO1, and STAT3. CAT and SOD2 activities were measured using biochemical assays. Multiplex immunohistochemistry was used to examine SIRT1-FOXO1 co-localization and FOXO1 nuclear localization. For the in vitro experiments, normal human gastric epithelial cells were treated with MNNG to induce inflammatory injury, followed by SJP administration in the presence or absence of the SIRT1 inhibitor EX-527. Results SJP alleviated gastric mucosal injury, reduced serum IL-6 levels and COX-2 expression, and significantly increased TFF1 and TFF2 expression. Metabolomics analysis identified 13 differential metabolites. The targets of these metabolites were intersected with gastritis-related targets, yielding 522 overlapping targets that were significantly enriched in the FOXO signaling pathway. In vivo and in vitro experiments showed that SJP upregulated SIRT1 and FOXO1 expression, increased CAT and SOD2 activities, inhibited STAT3 expression, and promoted FOXO1 nuclear localization. EX-527 blocked the restorative effects of SJP on FOXO1 expression and antioxidant enzyme activities. Conclusion SJP ameliorates gastric mucosal injury and inflammation, potentially through modulation of the SIRT1/FOXO1 axis.
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Animal Models and Experimental Medicine
·
2026
·
IF 3.3
Yuting Xu,Xuhong Li,Alvaro Munoz,Li Jiang,Lihua Huang,Yanhua Zhou,Liping Zhu,Youbo Yang
Background Stress urinary incontinence (SUI) is primarily caused by structural and functional damage to the external urethral sphincter (EUS). In previous studies, metformin, a known AMP-activated protein kinase (AMPK) activator, has shown repair effects after skeletal muscle injury. This study explored its therapeutic effect and molecular mechanisms in SUI associated with EUS injury. Methods The SUI model was established by double vaginal dilation. Rats were assigned to sham, vehicle-treated, or metformin-treated (200 mg/kg/day, oral) groups. Urodynamic testing was performed after 7 and 14 days. Subsequently, EUS tissues were isolated for histological (hematoxylin and eosin [H&E], Masson's), apoptotic (TUNEL), ultrastructural (transmission electron microscopy [TEM]), and molecular analyses. Finally, C2C12 myotubes cells were treated with varying concentrations of metformin to evaluate the expression of phosphorylated AMPK (p-AMPK) and MYH1/2, which are markers of skeletal muscle fiber type and regeneration. Results Abdominal leak point pressure (ALPP) and bladder leak point pressure (BLPP) decreased after double vaginal dilation, suggesting that SUI was established. After treatment for 7 and 14 days, respectively, metformin significantly increased ALPP and BLPP ( p < 0.05) without affecting maximum bladder capacity ( p > 0.05). Masson's staining and TEM revealed that metformin improved disorganized EUS architecture. Further, Western blot analysis demonstrated that metformin increased the expression of MYH1/2 and p-AMPK in vivo and in vitro. Meanwhile TUNEL assay showed that metformin treatment reduced apoptotic cells in the EUS from SUI rats. Conclusions These results indicate that metformin enhances EUS repair to improve SUI via activating the AMPK signaling pathway. These findings provide new insights into therapeutic strategies for SUI.
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International Journal of Nanomedicine
·
2026
·
IF 8.7
Pongsigeraxi Borjigin,Caixia Deng,Rilaga Su,Juan Li,Ying Ying,Yingsong Chen,Tegexibaiyin Wang
Purpose This study aimed to enzymatically synthesize pearl powder fluorescent carbon dots (PFCDs) and investigate their neuroprotective potential against cerebral ischemia/reperfusion injury (CIRI) by modulating the Anxa2/NF-κB signaling pathway.Methods PFCDs were synthesized through enzymatic digestion and characterized. Neuroprotective effects were assessed using an in vitro oxygen–glucose deprivation/reoxygenation (OGD/R) model in PC12 cells and a middle cerebral artery occlusion/reperfusion (MCAO/R) rat model. Cell viability, neurological function, cerebral infarct volume, and neuronal injury were evaluated. Expression of Anxa2/NF-κB signaling pathway proteins and inflammatory cytokines (TNF-α, IL-6, IL-1β) was analyzed by Western blot, immunofluorescence, and ELISA.Results The synthesized PFCDs exhibited an organic–inorganic hybrid structure, uniform particle size below 10 nm, and distinctive optical properties. In vitro, PFCDs enhanced cell viability under OGD/R conditions, inhibited phosphorylation of Anxa2 and NF-κB p65, and reduced secretion of pro-inflammatory cytokines (TNF-α, IL-6, IL-1β). In vivo, treatment with PFCDs in MCAO/R rats improved neurological function, reduced cerebral infarct volume, and alleviated neuronal injury. These protective effects were linked to downregulation of the Anxa2/NF-κB signaling pathway and reduced serum levels of inflammatory cytokines.Conclusion We successfully achieved the enzymatic synthesis of carbon dots from pearl powder, characterized by a unique organic–inorganic hybrid structure. PFCDs effectively alleviated CIRI-induced neuroinflammation by suppressing the Anxa2/NF-κB signaling pathway, highlighting their therapeutic potential as a nanomedicine derived from natural products.
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JOURNAL OF ETHNOPHARMACOLOGY
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2025
·
IF 5.4
Changqing Wen,Hang Su,Jiaxuan Li,Jia Zou,Mingxu Gong,Fujiang Wang,Haitao Ge
Ethnopharmacological relevance IgA nephropathy (IgAN) is characterized by the deposition of thylakoid Gd-IgA1 and progresses to kidney dysfunction and failure. Thousands of years ago, Abelmoschus manihot (L.) Medic has been used as a traditional Chinese medicine to treat kidney diseases. The total flavonoid (TFA) of Abelmoschus manihot (L.) Medic is the main active ingredient of the medicine, which is known to have therapeutic effects on kidney diseases. However, the mechanism of action is still not further explored. Aim of study We aim to reveal the targets and potential mechanisms of TFA through comprehensive proteomics and Weighted Gene Co-expression Network Analysis (WGCNA), and to provide new ideas for the treatment of IgAN. Materials and methods In this study, we constructed a model of IgA nephropathy in rats and evaluated the efficacy of TFA in the IgAN model by assessing renal function, renal Gd-IgA1/IgA and serum markers. Integrated proteomics and WGCNA analysis identified core targets associated with TFA, and target validation was performed by immunohistochemistry. Results Kidney function (Scr, PCR, BUN) and serum markers (TGF-β1, TNF-α, BAFF, MCP-1) were significantly reduced after TFA treatment. Proteomics identified 2,757 differential expressed genes (DEGs). WGCNA highlighted four key modules associated with TFA effects. Six core targets (C1QBP, CYC1, Phab, VDAC2, CDH2, Dbn1) were validated by immunohistochemistry, confirming their roles in TFA renoprotection. Conclusion Induction of rat IgAN model, proteomics and WGCNA analyses demonstrated that TFA improves renal function, reduces histopathological damage and modulates inflammation. These findings further elucidate the treatment mechanism of TFA and provide new insights into the treatment of IgA nephropathy.
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Integrative Zoology
·
2025
·
IF 3.7
Yunjuan Xiao,Xin Niu,Xueting Jiao,Liangping Lin,Haitao Shi,Meiling Hong,Li Ding
Social advancement and heightened waste discharge have resulted in escalating ammonia pollution in aquatic ecosystems, presenting toxicity hazards to aquatic fauna, especially turtles, by impairing their neurological function. To assess the ammonia effect on turtle brains, we subjected Chinese striped-necked turtles ( Mauremys sinensis ) to varying ammonia concentrations (CK, control; A1, 0.790 mg/L NH3; A2, 1.418 mg/L NH3) for durations of 24 and 48 h. Our data indicate that ammonia exposure markedly elevated glutamate levels and glutamate receptor mRNA expression in turtle brains, while concurrently diminishing glutamate transporter expression. These alterations resulted in an increase in brain water content, Na + -K + -Cl − cotransporter 1 (NKCC1), and matrix metalloproteinases. The decrease in tight junction proteins and Caveolin1 levels may lead to the rupture of the blood–brain barrier and subsequent edema. The impaired blood–brain barrier and edema led to elevated calcium levels and decreased function of certain ATPases. Gene expression associated with calcium homeostasis increased, signifying an imbalance. As the exposure time and concentration of ammonia increased, the TUNEL-stained positive cells began to appear. Taken together, increased ammonia concentrations lead to glutamate accumulation, impairing the blood–brain barrier and resulting in cerebral edema. This impairs calcium homeostasis, ultimately inducing cell death. This work provides significant insights into the toxicity of ammonia to aquatic turtles, hence augmenting our comprehension of stress physiology in these species. It underscores the necessity of safeguarding aquatic ecosystems from ammonia contamination to guarantee the health and survival of turtles and other aquatic fauna.
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JOURNAL OF CLINICAL ULTRASOUND
·
2026
·
IF 1.4
Xiangxiang Lu,Lizhen Li,Gencheng Liang,Wenlin Luo,Yan Liu,Zhaohe Huang,Chengcai Chen
Objective This study aimed to evaluate the cardioprotective effects of total flavonoids of Dragon's blood (TFDB) on myocardial ischemia–reperfusion injury (MIRI) using advanced ultrasound imaging techniques. Methods Twenty-four healthy New Zealand rabbits were randomly divided into observation (TFDB treatment) and control groups ( n = 12 each). The observation group received TFDB (180 mg/kg) orally for 14 days prior to model establishment, while the control group received physiological saline. MIRI was induced via ligation and recanalization of the left anterior descending coronary artery. Hemodynamic parameters, including heart rate (HR) and left ventricular systolic pressure (LVSP), were recorded at baseline (t0), immediate reperfusion (t1), and subsequent intervals up to 120 min (t5). Results Successful MIRI modeling was confirmed in 10 rabbits per group. TFDB-treated rabbits demonstrated significantly higher HR and LVSP at t4 and t5 ( p < 0.05). Serum creatine kinase-MB and lactate dehydrogenase levels were significantly lower in the TFDB group at t4 and t5 ( p < 0.05). At t5, the TFDB group showed significantly higher circumferential strain and ejection fraction values ( p < 0.05). Ultrasound microbubble imaging revealed significantly higher video intensity and myocardial blood flow (A × β) in the TFDB group at t5 ( p < 0.05). Conclusion TFDB demonstrated significant cardioprotective effects in MIRI. Ultrasound microbubble imaging combined with speckle tracking technology provides an effective approach for assessing these cardioprotective effects.
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FOOD CHEMISTRY
·
2025
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IF 9.8
Xiuqing Zheng,Mengting Huang,Jiaxu Liu,Binwang Yang,Jianna Yu,Wen Liu,Wenshan Li,Guoxing Jing,Wenjie Liu
Ethyl carbamate (EC), a carcinogen in alcoholic beverages, poses a potential hazard to human health. This study developed a gas chromatography-Fourier deconvolution ion mobility spectrometry (GC-FDIMS) method with ammonia dopant for EC quantification. The anhydrous MgSO 4 dehydration-assisted dichloromethane extraction of EC in alcoholic beverages simplified the sample pretreatment. The IMS system was established by the FD technique to improve the resolution and sensitivity. The results showed that the limit of detection (LOD) and limit of quantification (LOQ) for EC were 0.34 ng/mL and 1.16 ng/mL. The RSDs of the intraday precision and interday precision were in the range of 2.72 %–13.74 %, and the average recovery was between 81.93 % and 100.58 %. The developed method exhibited satisfactory quantitative performance for EC in various alcoholic beverage samples (beer, yellow rice wine, rice wine, and red wine) with the advantage of high sensitivity, fast separation, good specificity, and low cost.
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Frontiers in Pharmacology
·
2023
·
IF 5.6
E. Sun,Xiangqi Meng,Zhaoxia Kang,Huimin Gu,Mingyu Li,Xiaobin Tan,Liang Feng,Xiaobin Jia
Lung cancer is a common malignant tumor in clinical practice, and its morbidity and mortality are in the forefront of malignant tumors. Radiotherapy, chemotherapy, and surgical treatment play an important role in the treatment of lung cancer, however, radiotherapy has many complications and even causes partial loss of function, the recurrence rate after surgical resection is high, and the toxic and side effects of chemotherapy drugs are strong. Traditional Chinese medicine has played a huge role in the prognosis and improvement of lung cancer, among them, Zengshengping (ZSP) has the effect of preventing and treating lung cancer. Based on the “gut-lung axis” and from the perspective of “treating the lung from the intestine”, the purpose of this study was to research the effect of Zengshengping on the intestinal physical, biological, and immune barriers, and explore its role in the prevention and treatment of lung cancer. The Lewis lung cancer and urethane-induced lung cancer models were established in C57BL/6 mice. The tumor, spleen, and thymus were weighed, and the inhibition rate, splenic and thymus indexes analyzed. Inflammatory factors and immunological indexes were detected by enzyme-linked immunosorbent assay. Collecting lung and colon tissues, hematoxylin and eosin staining was performed on lung, colon tissues to observe histopathological damage. Immunohistochemistry and Western blotting were carried out to detect tight junction protein expression in colon tissues and expression of Ki67 and p53 proteins in tumor tissues. Finally, the feces of mice were collected to investigate the changes in intestinal microbiota using 16SrDNA high-throughput sequencing technology. ZSP significantly reduced tumor weight and increased the splenic and thymus indexes. It decreased expression of Ki67 protein and increased expression of p53 protein. Compared with Model group, ZSP group reduced the serum levels of interleukin (IL)-1β, IL-6, tumor necrosis factor α (TNF-α), and ZSP group increased the concentration of secretory immunoglobulin A (sIgA) in the colon and the bronchoalveolar lavage fluid (BALF). ZSPH significantly increased the level of tight junction proteins such as ZO-1, Occludin and Claudin-1. Model group significantly reduced the relative abundance of Akkermansia ( p < 0.05) and significantly promoted the amount of norank_f_ Muribaculaceae, norank_f_ Lachnospiraceae ( p < 0.05) compared with that in the Normal group. However, ZSP groups increased in probiotic strains ( Akkermansia ) and decreased in pathogens ( norank_f_ Muribaculaceae, norank_f_ Lachnospiraceae). Compared with the urethane-induced lung cancer mice, the results showed that ZSP significantly increased the diversity and richness of the intestinal microbiota in the Lewis lung cancer mice. ZSP played an important role in the prevention and treatment of lung cancer by enhancing immunity, protecting the intestinal mucosa and regulating the intestinal microbiota.
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TALANTA
·
2023
·
IF 6.1
Feng Luo,Yue Tang,Jia Zheng,Zhengmin Xie,Junjun Wang,Jianli Zhou,Yuangen Wu
Carbendazim (CBZ) is a widely used pesticides, and its excessive intake is serious damage to humans and animals. Herein, a stable and sensitive colorimetric aptasensor for rapid detection of CBZ residue has been established based on the enhancement of CBZ-specific aptamer (CZ-13) on oxidase-mimicking activity of octahedral Ag 2 O nanoparticles (NPs). The CZ-13 aptamer can significantly increase the catalytic activity by promoting the production of superoxide anion (·O 2 − ) on the surface of Ag 2 O NPs and enhancing the affinity of octahedral Ag 2 O NPs to 3,3′,5,5′-tetramethylbenzidine (TMB) molecules. In the presence of CBZ, the quantity of CZ-13 aptamer will be exhausted due to the specific binding to CBZ pesticide. Thus, the rest CZ-13 aptamer no longer enhanced the catalytic activity of octahedral Ag 2 O NPs, which leads to the change in color of sensing solution. The color change of sensing solution can be easily converted to the corresponding RGB value by a smartphone for quantitative and rapid detection of CBZ. The designed aptasensor has excellent sensitivity and specificity, and the limit of detection was determined as low as 7.35 μg L −1 for CBZ assay. Besides, the aptasensor exhibited good recoveries in the spiked cabbage, apple and cucumber, showing that it may have broad application prospects for detecting CBZ residues in agriculture products.
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Bioactive Materials
·
2023
·
IF 18.9
Bowen Zheng,Peng Zhang,Qijun Lv,Tao Wu,Yadong Liu,Junjie Tang,Yanping Ma,Lili Cheng,Langtao Xu,Yizhen Wang,Yifan Xue,Jie Liu,Jie Ren
Image-guided thermal ablation (TA), which is less invasive, has been widely applied for treating various kinds of tumors. However, TA still poses the potential risk of thermal damage to sensitive tissue nearby. Therefore, an adjunctive thermoprotective hydrodissection technique with constant injection of 5% glucose (5% Glu) has currently been adopted for clinical application, but this may be hazardous to humans. In this study, a multifunctional hyaluronic acid-based hydrogel (HA-Dc) was developed for hydrodissection. Compared with 5% Glu (the most clinically used solution) and the previously reported F127 hydrogel, the HA-Dc hydrogel was studied in vitro in a porcine liver model and in vivo in a rabbit model and showed good injectability and better tissue retention, stability, and thermoprotective properties throughout the TA procedure. Furthermore, in the preclinical evaluation in a Macaca fascicularis (M. fascicularis) model, HA-Dc showed excellent performance in terms of stricter neuroprotection compared with 5% Glu. In addition, the HA-Dc hydrogel with good biocompatibility and controllable degradation behavior in vivo could be a promising platform for thermal protection during clinical TA procedures.
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ACS Applied Nano Materials
·
2022
·
IF 6.14
Hui-Jun Fu,Lin Luo,Yu Wang,Cheng-Long Wang,Hong Wang,Yu-Dong Shen,Hong-Tao Lei,Niko Hildebrandt,Zhen-Lin Xu
Acrylamide is a probable human carcinogen with a wide occurrence in commonly consumed food. Because of the lack of specific antibodies against acrylamide, immunoassays usually measure acrylamide derivates, which require long derivatization times at elevated temperatures. One exception is xanthylacrylamide (XAA), which can be derived from acrylamide at room temperature within 30 min. Here, we present the development of a monoclonal antibody against XAA and a competitive enzyme-linked immunosorbent assay (ELISA) based on the multicolor detection of gold nanorods (AuNRs). The biotinylated monoclonal antibodies bound streptavidin alkaline phosphatase, which induced XAA concentration-dependent silver deposition on the AuNRs. The resulting blue shift of the AuNR surface plasmon resonance peak translated into a strong gray-to-orange color change that could be conveniently distinguished by the naked eye or smartphone color detection. The assay showed a broad linear range (0.3–17.2 ng/mL), a low limit of detection (0.06 ng/mL), and a 3.6-fold sensitivity improvement compared to those of conventional tetramethylbenzidine-based ELISA. The analysis of several different food products showed excellent acrylamide concentration recoveries (84–102%) and confirmed its applicability as an effective tool for routine monitoring of acrylamide in food. Rapid and simple color analysis demonstrated a strong potential for implementation into direct consumer product testing.
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