ACS Food Science & Technology
·
2025
·
IF 2.8
Jun-Hui Du,Yu-Yun Xiao,Ying-Chao Guo,Wen-Qing Zhang,Guan-Ru Si,Zheng-Fang Wu,Chuan-Qiang Gao,Jie Mei,Yi-Long Ma,Zhao-Jun Wei
T2DM (Type 2 diabetes mellitus) is a chronic metabolic disease typically managed with medications that often cause undesirable side effects, highlighting the urgent need for safe and effective natural alternatives. In this study, Baijiu distillers’ grains (BDGs) from Xiaojiao Baijiu were used to screen and identify anti-α-glucosidase peptides. The fraction under 3 kDa of the hydrolyzate generated by alcalase demonstrated the most potent inhibitory activity. This fraction was subsequently separated by RP-HPLC, resulting in the most effective subfraction, F2–2, with an inhibitory rate of 77.33 ± 1.15%. Six potential antidiabetic peptides (AAPFYAL, LAYPQ, IRLPFNQI, INPALPW, ATPVFL, SSSSAVVG) were identified from F2–2, with AAPFYAL showing the highest inhibitory activity (IC50 = 363.77 μM). Molecular docking demonstrated that these peptides competitively bind to key residues of α-glucosidase (such as D327, D542) via hydrogen bonds, which was further confirmed by pharmacophore model analysis. Our findings demonstrate that Xiaojiao BDGs are promising hypoglycemic resources.
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高影响因子
PROCESS BIOCHEMISTRY
·
2025
·
IF 4
Yumei Feng,Yuanmin Zhu,Bohan Wang,Feifan Zhao,Yanxia Zhang,Xinqi Zhu,Huaixin Li,Longjiang Yu
Rapeseed meal hydrolysate (RSMH), is a low-cost, nitrogen-rich substrate with high potential for microbial fermentation. This study investigated the feasibility of using RSMH as an alternative nitrogen source to enhance docosahexaenoic acid (DHA) production in Schizochytrium sp. H016. Nutritional analysis revealed that RSMH contains abundant amino acids and phenolics, which are favorable for microbial growth and stress tolerance. Fermentation results showed that replacing yeast extract with RSMH significantly improved biomass accumulation, lipid production, and DHA yield. At 120 h, the maximum biomass, lipid, and DHA yields reached 45.80 g/L, 18.62 g/L, and 7.30 g/L, respectively, corresponding to increases of 23.96 %, 97.66 %, and 94.15 % compared to the control. RT-qPCR analysis revealed that RSMH upregulated key genes involved in glycolysis, fatty acid biosynthesis, triacylglycerol assembly, and antioxidant defense, which together promoted carbon flux toward lipid synthesis and improved oxidative stress resistance. Confocal microscopy further confirmed the increased accumulation of lipid droplets in RSMH medium. Fed-batch fermentation in a 5-L bioreactor yielded DHA and lipids of 15.51 and 33.81 g/L, respectively, with a final biomass of 63.80 g/L. These findings demonstrate that RSMH effectively substitutes yeast extract and upgrades rapeseed meal into a feedstock for DHA production.
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Food Chemistry-X
·
2025
·
IF 8.2
Qiong Wu,Xinru Wu,Zifei Wang,Zhitong Cai,Yonggang Dai
In this study, Lactiplantibacillus plantarum BNCC 339790 was employed to extract soluble dietary fiber (SDF) from peanut shells, followed by modification using ultrasonic, microwave, and ultra-high-pressure methods to investigate their effects on SDF properties. Ultra-high-pressure modification increased SDF's water-holding and oil-holding capacities by 1.74-fold and 2.07-fold, respectively. Characterization revealed decreased crystallinity and altered surface morphology with increased roughness and porosity after modification by the three methods. In addition, microwave-treated SDF exhibited better thermal stability. Ultrasonic treatment significantly enhanced SDF's inhibitory effects on pancreatic lipase (from 62.44 % to 83.33 %) and α-amylase (from 79.81 % to 94.94 %), demonstrated improved hypoglycemic and hypolipidemic potential. Furthermore, ultrasonically modified SDF exhibited significant anti-inflammatory activity by significantly suppressing TNF-α, IL-6, and IL-1β expression. This study provides a foundation for the further development of SDF and its promotion in functional foods and other potential applications.
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 2
·910
·
IF 6.208
Ji-Xin Li,Shu-Xiang Zhao,Yu-Qing Zhang
This paper describes the use of silk protein, including fibroin and sericin, from an alkaline solution of Ca(OH)2for the clean degumming of silk, which is neutralized by sulfuric acid to create calcium salt precipitation. The whole sericin (WS) can not only be recycled, but completely degummed silk fibroin (SF) is also obtained in this process. The inner layers of sericin (ILS) were also prepared from the degummed silk in boiling water by 120 °C water treatment. When the three silk proteins (SPs) were individually grafted with glycidyl methacrylate (GMA), three grafted silk proteins (G-SF, G-WS, G-ILS) were obtained. After adding I2959 (a photoinitiator), the SP bioinks were prepared with phosphate buffer (PBS) and subsequently bioprinted into various SP scaffolds with a 3D network structure. The compressive strength of the SF/ILS (20%) scaffold added to G-ILS was 45% higher than that of the SF scaffold alone. The thermal decomposition temperatures of the SF/WS (10%) and SF/ILS (20%) scaffolds, mainly composed of a β-sheet structures, were 3 °C and 2 °C higher than that of the SF scaffold alone, respectively. The swelling properties and resistance to protease hydrolysis of the SP scaffolds containing sericin were improved. The bovine insulin release rates reached 61% and 56% after 5 days. The L929 cells adhered, stretched, and proliferated well on the SP composite scaffold. Thus, the SP bioinks obtained could be used to print different types of SP composite scaffolds adapted to a variety of applications, including cells, drugs, tissues, etc. The techniques described here provide potential new applications for the recycling and utilization of sericin, which is a waste product of silk processing.Keywords:silk protein;fibroin;sericin;grafting;composite bioink;bioprinting;3D scaffolds;mechanical properties
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BIORESOURCE TECHNOLOGY
·
2022
·
IF 11.889
Xiang Zou,Junguo He,Pengfei Zhang,Xinlei Pan,Yijie Zhong,Jie Zhang,Xuewei Wu,Biqing Li,Xia Tang,Xiannian Xiao,Heliang Pang
This study introduced the excellent improvement of enzyme cocktail (lysozyme and protease) on hydrolysis efficiency and the role of reducing carbon emission as an alternative carbon source. The best dosing method after optimization was to add four parts of lysozyme at 0 h and one part of protease at 1 h. The extracellular proteins and polysaccharides increased by 118% and 64% respectively under the optimal dosing mode. Enzyme cocktails reduced more organic matters and extended the distribution of sludge particles in the small particle size part. The enzymatic-treated sludge could reduce 21.09 kg CO 2 /t VSS if utilized to replace methanol for denitrification carbon source. Enzyme cocktails did better in enhancing both solubilization and hydrolysis than single enzymes under the optimal method. This study will provide a more integrated and comprehensive system for enzymatic pretreatment and new insight into the enzymatic pretreatment enhancing hydrolysis and reducing carbon emission.
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Journal of Functional Foods
·
2022
·
IF 5.223
Yuzhe Huang,Kunfeng Zhang,Wenqiang Guo,Chan Zhang,Hao Chen,Tenglong Xu,Yongming Lu,Qingxi Wu,Yong Li,Yan Chen
Tartary buckwheat is a popular natural grain commonly used as a functional food in various countries. This study aimed to prepare buckwheat dietary fiber with improved biological activity using liquid fermentation of Aspergillus niger and to elucidate its physicochemical properties and related mechanisms. Compared with unfermented buckwheat dietary fiber, Aspergillus niger fermentation increased the soluble dietary fiber content, thereby improving dietary fiber's physical and chemical properties such as water, oil retention capacity, and swelling cholesterol-binding capacity. Fermented Tartary buckwheat dietary fiber treatment for 14 weeks significantly decreased the hepatic lipid profile, oxidative stress, and protein expression in mice. In addition, fermented Tartary buckwheat dietary fiber intervention could regulate high-fat diet-induced disorders of gut microbiota. These results suggested that fermented Tartary buckwheat dietary fiber has potential “prebiotic” effects in ameliorating obesity.
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JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
·
2021
·Vol. 59
·期 6
·2381-2391
·
IF 2.701
Zhai Xinyu,Ao Huanping,Liu Wenhui,Zheng Jiaxin,Li Xingjie,Ren Difeng
Rosa roxburghii pomace was treated by steam explosion (SE) at 0.87 MPa for 97 s. After SE treatment, the Insoluble dietary fiber (IDF) content of Rosa roxburghii pomace decreased from 45.13 ± 0.23 to 30.01 ± 0.15%, and the soluble dietary fiber (SDF) content increased from 9.31 ± 0.07 to 15.82 ± 0.31%. The structure of IDF and SDF after SE showed that the original compact structures were destroyed, and the specific surface areas increased. Thermal analysis showed that the thermal stability of the modified SDF was improved. However, SE did not change the crystal structure and functional group composition of IDF and SDF. Physicochemical analysis indicated that IDF had better hydration capacity after SE treatment, and the oil-holding capacities of IDF and SDF were also significantly improved. SE is an effective method to improve the utilization of Rosa roxburghii pomace and a feasible method for modification of dietary fiber.
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Reaction Chemistry & Engineering
·
2021
·Vol. 7
·期 2
·275-283
·
IF 4.239
Yan Zhang,Bi-Cong Wang,Po Wang,Xiao-Jie Ju,Mao-Jie Zhang,Rui Xie,Zhuang Liu,Wei Wang,Liang-Yin Chu
Uniform hydrogel microparticles with MOF nanoparticles for molecular co-confinement of cascade enzymes are developed by droplet microfluidics to achieve enhanced stability and reusability under harsh conditions. Water-swollen polyacrylamide hydrogel is used for constructing the microparticles; while microporous zeolitic imidazolate framework-8 (ZIF-8) nanoparticles are incorporated in the hydrogel networks for multi-enzyme co-confinement. With the ZIF-8 nanoparticles as armour, the multiple enzymes in the microparticles show good stability for efficient biocatalytic cascades under harsh conditions. This is demonstrated by using glucose oxidase (GOx) and horseradish peroxidase (HRP) as typical cascade enzymes. The hydrogel microparticles with both GOx and HRP confined in their ZIF-8 nanoparticles exhibit enhanced stability for efficient biocatalytic cascades and storage, after treatment under harsh conditions involving high temperature, UV light, and protease. Moreover, the hydrogel microparticles allow easy recycling and good reusability after repeated treatment at high temperature (80 °C). This work provides a simple and efficient strategy to create multiple-enzyme-loaded microcarriers with good stability and reusability for biocatalytic cascades.
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JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
·
2021
·Vol. 110
·期 4
·827-837
·
IF 4.396
Hai-Yan Wang,Su-Fang Zhou,Meng Zhang,Heng-Da Wang,Yu-Qing Zhang
Temperature and humidity (TH) are highly important factors that can control the secondary structure and characterization of silk fibroin (SF) biomaterials. In this study, the water stability, secondary structure, mechanical properties, surface morphology, and degradation of silk fibroin films (SFFs) with post-processing in different TH were investigated. Fourier transform infrared indicated that the SFF secondary structure did not change under low-relative humidity (RH) despite temperatures up to 180°C, while it transformed at 40°C with 100% RH in 10 min. A film with a higher tensile strength (42.1 ± 8.2 MPa) could be obtained after post-processing at 90°C/100% RH for 10 min. While a film with higher ductility (elongation at break: 198.8 ± 31.8%) was generated after post-processing at 40°C/100% RH for 10 min. Scanning electron microscope showed that the film presented a network structure of nanoparticles in series under certain TH post-treatment. Enzymatic hydrolysis proved that the SFFs containing a higher content of silk II structure degraded more slowly. Therefore, TH post-treatment is a relatively mild way to change the secondary structure and properties of SFFs, which can be widely used in loading drugs and maintaining the activity of drugs in SF biomaterials.
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FOOD CONTROL
·
2021
·
IF 5.548
Yaqi Hou,Jiao Liu,Yanchun Shao,Xitian Peng,Dawen Zhang,Lifang Hu,Fusheng Chen,Youxiang Zhou
Modified mycotoxins can cause an underestimation of exposure risk due to their untraceable characteristics. Hongqu , a traditional Chinese fermented product, is widely used as food colorants , fermenters and functional foods for about 2000 years. Though there were several methods to reduce citrinin (CIT) content in Hongqu , the risk assessment and prevention of modified CIT remain inadequate, causing potential risk to human health. The current study firstly identified the occurrence of modified CIT in Hongqu via acid, alkaline and enzyme hydrolysis combined with chromatographic and mass spectrometric approaches. Then 8 Hongqu samples and 5 Monascus products were treated using the optimized acid hydrolysis method and analyzed by ultra-high-performance liquid chromatography with fluorescence detector. The results showed that CIT content in the CIT positive Hongqu increased by 35.28%–458.13% after acid hydrolysis, demonstrating there could be a underestimation risk in the CIT positive Hongqu samples, and the modification of CIT might not be chemical modified, but matrix associated.
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FOOD HYDROCOLLOIDS
·
2020
·
IF 7.053
Tao Yang,Hui-Ling Yan,Chuan-He Tang
The utilization of okara , an important byproduct of soybean industry, has attracted increasing interests, due to its good nutrition and potential health effects. This work investigated the influence of a wet media planetary ball milling on some physicochemical and functional properties and cholesterol binding capacity (CBC) of okara from defatted soy flour . The tested properties included particle size, ζ -potential, water holding and swelling capacities (WHC and WSC), oil binding capacity (OBC), cation exchange capacity (CEC), emulsifying ability and emulsion stability indices (EAI and ESI), foaming ability (FA) and foam stability (FS). The results indicated that a milling with increasing time from 5 to 15 h resulted in a progressive decrease in z -average diameter (up to 270 nm) of okara particles, and thus improved their water dispersibility. In general, the milling progressively increased WHC , WSC, OBC, CEC, EAI, FA and CBC, in a milling time dependent way. Especially for the okara milled for 15 h, its EAI became comparable to that of soy protein isolate (SPI), while its ESI was considerably higher than SPI. The CBC of okara at pH 7.0 increased by 3-folds, after the milling for 15 h. Such improvements of functionalities were largely attributed to the transformation of insoluble components into water-dispersible, highly functional colloidal nanoparticles , as well as disruption of crystalline polysaccharides for milled okara. The findings are of interest not only for guiding the highly-added-value utilization of okara, but also for the development of a kind of highly functional and health-benefiting food functional ingredients with a great potential to be applied in food formulations.
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高影响因子
FOOD CONTROL
·
2021
·
IF 5.548
Hongxia Tan,Hongyuan Zhou,Ting Guo,Yuhao Zhang,Liang Ma
Zearalenone (ZEN) is one of the most polluted mycotoxins in maize and maize-products. To date, however, previous studies have only focused on the presence of free ZEN. To confirm the occurrence of hidden ZEN in maize and maize-products, the samples were pretreated using different hydrolysis methods and in vitro digestion. In this study, significantly increased amount of total detectable ZEN was found by the hydrolysis with compound protease and especially in vitro digestion, which indicated the presence of hidden ZEN in maize. The hidden ZEN can be converted into its free form by in vitro digestion and then be found/detected out. Especially, zein-bound zearalenone was the main “hidden mode” for the presence of hidden ZEN. The amount of total detectable ZEN in digested zein was 7 times higher than that in undigested zein. And also, fluorescence quenching and ultrafiltration studies also showed that ZEN can bind with zein spontaneously by non-covalent bonds. Overall, this study aroused an important issue for risk assessment that the total amount of ZEN in maize products might be underestimated due to the presence of zein-bound ZEN.
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危险品化学品经营许可证（带存储）