RSC Advances
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2016
·Vol. 6
·期 35
·29638-29645
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IF 3.289
Xin Ge,Xinzhi Chen,Chao Qian,Shaodong Zhou
Ullmann type C–N coupling reaction catalyzed by copper(I) with D-glucosamine derivatives as promoters was studied by means of combined experimental/theoretical investigation. The catalytic role of D-glucosamine was addressed. In contrast with previous speculations, the amino group may not count in the catalytic cycle in which the oxidative addition/reductive elimination mechanism works. Experimental results are in good agreement with theoretical findings. Extensive work indicates the wide applicability of the C–N coupling strategy exploited in this work.
研究领域:
有机化学
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催化化学
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药物化学
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计算化学
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合成方法学
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生物正交化学
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金属有机化学
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JOURNAL OF MOLECULAR LIQUIDS
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2022
·
IF 6.633
Hongjun Zang,Yimo Feng,Jing Lou,Kai Wang,Changchun Wu,Zhipeng Liu,Xi Zhu
Ionic liquids have shown good catalytic performance and been widely used in the biomass conversion process due to their unique properties, including environmental friendship, low toxicity, and simple preparation. In this work, a series of novel piperidinium-based ionic liquids were synthesized under mild conditions via a simple protonation reaction or nucleophilic substitution reaction. Among them, 4,4′-(propane-1,3-diyl)bis(1-(carboxymethyl)piperidin-1-ium) chloride ([PDCMPi]Cl) displayed the best catalytic performance. The highest yield (42.81 %) of 3A5AF from NAG was obtained after reacting in N-methyl-pyrrolidone (NMP) in the presence of piperidinium-based ionic liquids at 180 °C for 20 min under normal atmospheric conditions. Additionally, ionic liquid [PDCMPi]Cl showed good catalytic activity in six repeated catalytic runs. Meanwhile, a possible reaction pathway was also proposed on the basis of the results of NMR spectra.
研究领域:
催化化学
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绿色化学
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生物质能源
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有机合成
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离子液体化学
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Food Chemistry-X
·
2026
·
IF 9.2
Yu Wang,Sharuna De,Hui Chu,Yujun Jiang,Jia Shi
This study investigates the effects of transglutaminase (TGase)-mediated glycosylation on the structure, functional properties, and intestinal barrier function of whey protein isolate (WPI). Structural analysis revealed that the covalent interaction between glucosamine and WPI led to a more stable and ordered protein structure. Compared with WPI, the glycosylated WPI exhibited improved foaming, emulsifying, and antioxidant properties. TGase-mediated glycosylation also reduced the degree of hydrolysis of WPI under in vitro digestion conditions. Furthermore, a cellular intestinal barrier model was established using Caco-2 cells, and treatment with digested glycosylated WPI significantly increased transepithelial electrical resistance while decreasing paracellular permeability. RT-PCR and Western blot analyses demonstrated that the digest of glycosylated WPI up-regulated the expression of tight junction proteins (Occludin, Claudin-1, and ZO-1), thereby enhancing intestinal barrier function. This study provides new insights into the development of food proteins that combine desirable functional properties with biological activities.
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CHEMISTRY & BIODIVERSITY
·
2026
·Vol. 23
·期 3
·e03369
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IF 2.9
Xiaoliang Zhao,Lixia Xiong,Haidong Wen,Yuanyuan Ma,Yuxin Wang,Cunjin Wang,Pengcheng Shao,Jing Zhang,Yuhui Zhao,Ji Zhang,Weijie Zhang
The extraction methods significantly affect the yield and structure of polysaccharides. Based on single-factor experiments, response surface methodology optimized ultrasound-assisted extraction conditions for corn silk polysaccharides (CSP): liquid-to-solid 43 mL/g, extraction time 3 h, and ultrasonic power 640 W, yielding 5.83% CSP. Sulfated, phosphorylated, and selenylated derivatives (CSP-S, CSP-P, and CSP-Se) were prepared with substitution degrees of 0.6 and 0.4, and selenium content of 5.3%, respectively. After confirming structures by UV, FT-IR, SEM, monosaccharide, and Congo-red analyses, the bioactivities of the modified CSP were compared. All derivatives improved antioxidant capacity in vitro: phosphorylation boosted DPPH scavenging, while sulfation enhanced hydroxyl and superoxide anion radical elimination. Selenylation markedly inhibited the activities of α-amylase and lipase, and promoted glucose consumption in HepG2 cells, demonstrating hypoglycemic potential. Moreover, all polysaccharides enhanced RAW264.7 cell viability, phagocytosis, and nitric oxide (NO) release, with CSP-S being most effective. Chemical modifications markedly improved CSP bioactivities, highlighting their application prospects. Graphical
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ACS Applied Materials & Interfaces
·
2026
·
IF 7.8
Sifan Sun,Jiawei Li,Qianzuo Liu,Binqi He,Haoran Yang,Xinyue Xiang,Ying Hao,Xuehua Yan,Ting Zhang,Tie Li
Hydrogen peroxide (H2O2) is an indispensable mediator in cellular signaling pathways, playing a multifaceted role in biological systems. The development of advanced catalysts derived from flexible sensors for the real-time monitoring of dynamic H2O2 in vivo remains an important research issue. Recently, dual single-atom catalyst-based nanozymes exhibit synergistically enhanced catalytic performance, displaying excellent potential for H2O2 detection. Nevertheless, rationally regulating the geometric structures and electron transfer between the two metals still pose significant challenges. Hence, in this study, an advanced Fe/Ce dual single-atom catalyst (Fe/Ce–C)-based nanozyme was constructed, which proposes a Ce single-atom doping strategy to alter the electronic structure of Fe single-atom sites, endowing it with a high adsorption energy for hydroxyl groups to reduce the energy barrier of H2O2 in the reaction. This Fe/Ce–C achieves a linear sensing behavior in the range of 0.5–10,000 μM with a response speed of 1.4 s, a detection limit of 0.223 μM, and a sensitivity of 213 μA/mM·cm2. Furthermore, the nanozyme-derived flexible electrochemical sensor was assembled for real-time monitoring of H2O2 concentration changes in the brains of living rats, exhibiting long-term detection capability and excellent in vivo biocompatibility. Such an Fe/Ce dual single-atom nanozyme-based electrochemical sensor can serve as a promising tool for monitoring diseases like Parkinson’s.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2026
·
IF 8.5
Chang Liu,Wanting Zhang,Meng Li,Jinjing Chen,Yiming Chen
This study primarily focuses on the utilization of Cyperus esculentus , which is mainly used for oil extraction. The Cyperus esculentus protein (CEP) hydrolysates glycosylated product was prepared under the mediation by transglutaminase (TGase) with glucosamine. Response surface methodology was used to optimize the preparation process for the glycosylated products, with the optimal conditions being a TGase addition of 21 U/g, a ratio of protein hydrolysate to glucosamine of 3:1, and a reaction time of 1.5 h.The degree of grafting for the glycosylated products was 40.72%. The glycosylation product prepared under this condition exhibited excellent emulsifying activity index (EAI 64.75 m 2 /g), which was slightly lower than the theoretical value of 66.15 m 2 /g. It can be attributed that the actual reaction time was longer than the theoretical time, and the slight rise in viscosity of the reaction mixture has caused some aggregation, leading to a minor reduction in the emulsifying property of the glycosylated product. Infrared spectroscopy, fluorescence spectroscopy, UV - visible absorption spectroscopy and NMR results indicated that the internal groups of Cyperus esculentus protein changed after enzymatic hydrolysis and glycosylation, confirming the occurrence of both processes. The thermal stability of the glycosylated products has been enhanced, and their antioxidant properties have also been improved. This study provided a new option of protein emulsifier based on plants and supplemented methods and theories for protein modification. The obtained glycosylated protein emulsifier has potential research value in applications of food processing, cosmetic manufacturing and drug encapsulation, etc.
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CARBOHYDRATE RESEARCH
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2026
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IF 2.5
Jiaying Sun,Le Sha,Guangyu Zhu,Linquan Li,Pengfei Li,Mengran Chen,Junhao Wang,Xueying Jiao,Na Gao,Jinhua Zhao
Free radical depolymerization has been widely employed for polysaccharide structural and functional studies. However, H 2 O 2 -induced depolymerization of the unique sea cucumber derived fucosylated chondroitin sulfate (FCS) generated diverse fragments with oxidation at their reducing ends, thus limiting preparation of well-defined oligosaccharides. Here, a thermal dissociation initiator, potassium persulfate (KPS), was utilized to induce radical depolymerization of FCS, leading to depolymerized products that maintained their basic structural units and sulfate substituents. Structural analysis of the resulting oligosaccharides revealed that the reducing ends were uniformly GalNAc 4S6S acid, distinct from those generated by H 2 O 2 -induced FCS depolymerization. The major components, tetrasaccharide (FIIIa), heptasaccharide (FVa) and decasaccharide (FVIa) possessed GalNAc 4S6S residues at their non-reducing ends, demonstrating that the D-GlcA-β1,3-D-GalNAc 4S6S linkage was preferentially cleaved. A free radical depolymerization mechanism of FCS by KPS was proposed. The regular aldonic acid terminal formation was attributed to the strong oxidative capacity of the sulfate radical for extensive oxidation of the newly formed GlcA residue after β1,3 glycosidic linkage cleavage, and further oxidation of C-1 of GalNAc. KPS was thermally dissociated to generate sulfate radicals, which subsequently attacked FCS chains and led to their fragmentation. Moreover, biological activity assay showed that heptasaccharide FVa and decasaccharide FVIa demonstrated strong anticoagulant activity, primarily via iXase inhibition mediated by high-affinity interaction with FIXa. Intriguingly, the unique oxidized reducing end exhibited enhanced iXase inhibitory activity. Hence, our findings expanded the free radical depolymerization of FCS and enriched structure-activity relationship information for iXase inhibition.
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NEW JOURNAL OF CHEMISTRY
·
2023
·Vol. 47
·期 11
·5420-5430
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IF 3.3
Qi Guo,Jihong Xu,Rui Tang,Yulin Min,Zhenhu Hu,Penghui Shi
In the degradation process of organic pollutants, compared with the free radical pathway, the non-radical pathway shows the advantages of mild oxidation ability, strong anti-interference, and adaptation to different pH values. In this study, a manganese–nitrogen co-doped porous carbon (Mn–N@C-1) with high nitrogen content was prepared to improve the contribution rate of non-radical pathways to the degradation process of organic pollutants. The high performance of Mn–N@C-1 is attributed to the formation of Mn–Nx sites between Pyridine N and Mn, which can effectively oxidize organic pollutants in water (phenol, bisphenol A, 2,4-dichlorophenol). In addition, the catalyst exhibits high catalytic activity over a wide pH range (pH = 3–9) and has good immunity to inorganic anions. Moreover, the Mn–Nx site and graphitic-N are considered to be the generation sites of singlet oxygen, and the role of metastable Mn–N@C-1/PMS* complexes in the electron transfer process is explored. Overall, this study explores the crucial role of non-radical pathways in the degradation of organic pollutants by carbon-based materials.
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FOOD CHEMISTRY
·
2022
·
IF 9.231
Cui Cao,Lilong Wang,Xueqian Zhang,Chunqing Ai,Zhongfu Wang,Linjuan Huang,Shuang Song,Beiwei Zhu
The present study investigated the utilization of an arabinogalactan from Lycium barbarum (LBP-3) by intestinal Bacteroidetes species. The mixed-culture assay showed 58.4 % LBP-3 was utilized, and Bacteroides caccae and Phocaeicola vulgatus utilized more LBP-3 in single-culture compared to others. During in vitro fermentation of LBP-3, P. vulgatus favored arabinose while B. caccae preferred galactose. Moreover, 9 and 25 oligosaccharides were identified by HPLC-MS n in conditioned media (CM) derived from B. caccae and P. vulgatus , respectively. All of 3 tested Parabacteroides species ( P. distasonis , P. goldsteinii , and P. johnsonii ) markedly proliferated in CM of B. caccae and P. vulgatus , and proliferations of B. uniformis , B. finegoldii , B. ovatus and B. thetaiotaomicron also increased significantly in CM of B. caccae. The study suggests that the ability of Bacteroidetes species to degrade LBP-3 and sheds light on cooperative interactions of Bacteroides, Phocaeicola , and Parabacteroides species in the presence of LBP-3.
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FITOTERAPIA
·
2023
·
IF 3.4
Yang Liu,Limei Ran,Yahong Wang,Peng Wan,Hongli Zhou
The polysaccharides from Sea buckthorn leaves (SBLPs) were extracted by hot water and purified by DEAE cellulose , then separated into six polysaccharides (SBLP-S) by DEAE-52 column. Six separated polysaccharides were characterized by Ultraviolet Spectroscopy, Infrared Spectrum, High Performance Liquid Chromatographic and Congo red analysis. The antioxidant activity and immunological activity were investigated in vitro . The results revealed that the monosaccharide composition of SBLP-S-1, SBLP-S-2, SBLP-S-3, SBLP-S-5 and SBLP-S-6 contained Man , GlcN , Rib , Rha , GluA , GalA , Glu, Gal, Xyl , Ara and Fuc , among them, rare glucosamine was found. And SBLP-S-4 contained all above components except GlcN and GluA. FT-IR showed that SBLP-S were sulfated polysaccharide containing uronic acid . Molecular weights of SBLP-S were 338.659, 401.305, 599.849, 393.904, 626.895 and 176.862 kDa. The Congo-red test indicated that SBLP-S-2, SBLP-S-4, SBLP-S-5, and SBLP-S-6 had triple helix conformation. Crude polysaccharides had the strong scavenging activities on DPPH radicals, ABTS radicals and hydroxyl radicals . The six polysaccharides had the activity of immune stimulation on RAW264.7 cell. SBLP-S-2 promoted the phagocytosis best and SBLP-S-6 promoted the NO production best. The results suggested that SBLPs could be used as potential antioxidants and immunomodulatory agents in pharmaceutical and functional food fields.
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Polymers
·
2022
·Vol. 14
·期 21
·4636
·
IF 4.967
Zhengyu Hu,Jiaming Wang,Long Jin,Tieqiang Zong,Yuanqi Duan,Jinfeng Sun,Wei Zhou,Gao Li
This investigation focuses on the three novel polysaccharides fromCordyceps militarisand then discusses their characterization and anti-complementary activity. The three polysaccharides fromC. militaris(CMP-1, CMP-2 and CMP-3) were prepared using a DEAE-52 cellulose column. The HPLC, HPGPC, FT-IR and Congo red analyses were used to characterize their monosaccharides, molecular weight and stereo conformation, which demonstrated that the three polysaccharides were homogenous polysaccharides with different molecular weights and were composed of at least ten monosaccharides with different molar ratios, and all had a triple-helix conformation. The evaluation of anti-complementary activity demonstrated that the three polysaccharides significantly inhibited complement activation through the classical pathway and alternative pathway. Preliminary mechanism studies indicated that CMP-1, CMP-2 and CMP-3 acted with C2, C5, C9, factor B, factor B, and P components in the overactivation cascade of the complement system. The analysis of the Pearson correlation and network confirmed that the ribose, glucuronic acid and galacturonic acid composition were negatively correlated with the anti-complementary activity of polysaccharides. These results suggested that the three novel polysaccharides are potential candidates for anti-complementary drugs.Keywords:Cordyceps militaris;anti-complementary activity;polysaccharides;structure-activity relationship
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INDUSTRIAL CROPS AND PRODUCTS
·
2022
·
IF 6.449
Dongxue Cao,Enwei Wei,Zeyu Wang,Zhengyu Hu,Ling Qi,Hongli Zhou,Jingli Zhao
This paper aimed to elaborate the chemical characteristics of ginseng polysaccharide via chemical methods and instrumental analyses; additionally, the anti-glioma activity and immunostimulatory capacity were also evaluated via biological experiments. Ginseng polysaccharide by microwave-assisted extraction (MEGP) method was purified by spongiform diethylaminoethyl cellulose (DEAE) and further separated by DEAE-52 column chromatography to obtain four MEGP fractions (MEGP-0, MEGP-1, MEGP-2, MEGP-3). Utilizing Zeta potential analyzer and X-ray photoelectron spectroscopy (XPS), MEGPs were, for the first time, confirmed the valence state of C and O with the binding mode of elements, and all MEGP fractions contained triple-helix structure. MEGP-2 and MEGP-3 significantly reduced the cell growth rate of TJ905 and U87 , showing an inhibitory effect on glioma. Among four fractions, MEGP-2 markedly elevated the immunostimulatory capacity via the activation of MAPK signaling route, with an increasing the expression levels of p-p38, p-ERK and p-JNK. In summary, this paper laid a solid foundation for the development of ginseng, as a promising drug or tonic, in the fields of anti-glioma and immunoregulation.
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