AMINO ACIDS
·
2025
·Vol. 57
·期 1
·1-15
·
IF 2.4
Wang Jiteng,Zhang Jingyi,Li Xinyu,Xu Hanying Y.,Yang Yeshun,Zhang Jiankun,Feng Wenping,Chen Qiang,Dong Fen,Han Tao
The main objective of this experiment was to study the metabolism of arginine in juvenile largemouth bass ( Micropterus salmoides ). A total of 300 healthy fish (average weight of 25 ± 0.5 g) were randomly assigned to ten groups. Experimental fish were orally administered or intraperitoneally injected with 0.9% sodium chloride, arginine, arginine-aspartate, citrulline, and glutamate solutions, respectively. They were euthanized at 10, 30, 60, 120, and 240 min after oral administration or intraperitoneal injection, and various tissue samples were subsequently collected for analysis. The results revealed that serum ornithine and citrulline concentrations of largemouth bass were significantly increased by oral administration of arginine or arginine-aspartate ( P < 0.05). Intraperitoneal injection of arginine or arginine-aspartate solution significantly elevated the concentrations of ornithine and citrulline in the serum, liver, kidney, and muscles ( P < 0.05). The concentrations of citrulline, ornithine, and arginine in serum and muscle increased significantly at 4 h after intraperitoneal injection of glutamate ( P < 0.05). Intraperitoneal injection of citrulline significantly increased the concentrations of ornithine and arginine in the serum and muscles ( P < 0.05). The research findings demonstrate that both free and small peptide forms of arginine were rapidly degraded to ornithine due to the high arginase activity in various tissues of largemouth bass. Additionally, the pathway of synthesizing citrulline from glutamate and then arginine from citrulline may exist in largemouth bass, but the exact location of this synthesis process may differ from that found in mammals.
研究领域:
水产养殖学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF SOLID STATE CHEMISTRY
·
2023
·
IF 3.3
Wenjing Yan,Bin Liang,Wanhe Li,Hualin Huang,Deqiang Shi,Zhenming Chen,Zhongmin Li,Meihua Yu,Guangtao Wei,Kai Huang
The formation of vaterite is well known to be influenced by many low molecular weight additives. In this study, Density functional theory calculations was employed to study the effect of biological material lysine(Lys), aspartic acid(Asp), threonine(Thr) and glycine(Gly) on the formation of vaterite CaCO 3 . The interaction energies between Gly and CaCO 3 (110) and (104) surfaces were −2.618 and −1.784 eV, respectively. With the molar ratio of Ca 2+ to Gly at 1.5, the reaction time of 11.5 min, the pH value of 7.5, the crystalline product of the reaction was almost monodisperse spherical vaterite in Ca(OH) 2 -Gly-CO 2 reaction system. The obtained products were characterized by the XRD, RAM, SEM, TEM, and FT-IR. The results showed that the increase of Gly concentration inhibited the nucleation and the growth of calcite. Lys, Asp, Thr and Gly were employed as stabilizers for controlling particles, the crystalline product of the reaction was almost monodisperse vaterite CaCO 3 . Its surface chemistry can be explored using this mechanism analysis. The methods are expected to be a reference for industrial production process.
研究领域:
材料科学
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生物化学
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产品关联
DOI
高影响因子
Food Bioscience
·
2022
·
IF 5.318
Chang Liu,Yahui Guo,He Qian,Yuliang Cheng
Inulin can regulate the intestinal micro-ecological balance, but there are few studies on the changes of intestinal microbiota and its metabolites in vitro and vivo , SCFAs and amino acids in particular. In this study, the human fecal microbiota glycolysis model and a mouse model were used to study the mechanism of inulin on intestinal microecology. Combined with metabolism of microbiome and metabolome , the cross-synergism mechanism of inulin-micribiota-metabolites-metabolism was investigated. The results revealed that after inulin was fermented by human intestinal microbiota , the relative abundance of Lactobacillus , Bifidobacterium , Prevotella , Catenibacterium , and Agathobacter increased. In vivo , the concentration of SCFAs increased after inulin was fermented, and the abundance of Parabacteroides , Muribaculaceae and Alloprevotella increased. Both in vivo and in vitro experiments revealed that inulin can regulate the expression of 5 genes involved in Valine , leucine and isoleucine degradation. In addition, the metabolome results confirmed that Valine, leucine and isoleucine degradation, Tyrosine metabolism, and Synthesis and degradation of ketone bodies are regulatory pathways of inulin. More importantly, L−tryptophan, tyrosine, L−fucose, L−ornithine, D−fructose, D−erythrose, and pyruvic acid are hallmark metabolites of inulin metabolized by gut microbiota. The cross-synergism network greatly indicated the microbiota ( Streptococcus , Lactobacillus , Muribaculaceae), metabolites ( l -tryptophan, pyruvic acid), and metabolism (Lysine degradation, Tryptophan metabolism , Synthesis and degradation of ketone bodies) may be the key factors involved in the mechanisms regulating the in vitro and vivo effects of inulin.
研究领域:
微生物学
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代谢组学
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营养科学
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胃肠病学
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产品关联
DOI
高影响因子
POULTRY SCIENCE
·
2020
·
IF 2.659
Mingfa Sun,Hongchao Jiao,Xiaojuan Wang,Victoria A. Uyanga,Jingpeng Zhao,Hai Lin
Crystalline amino acids ( AAs ) exhibit high nutritional values when supplemented AA-deficient diets. However, the AAs in crystalline form in the diet are absorbed quickly than protein-bound AAs, which may take an effect on AA utilization efficiency. In this study, 2 experiments were conducted to investigate the effect of encapsulated lysine-HCl ( Ly s) and DL-methionine ( DL-Met ) on the growth performance of broiler chickens. In experiment 1, a total of 432 one-day-old male Arbor Acres broilers were subjected to 3 dietary treatments (27 pens; 16 birds per pen) for 42 d. The control group was basal diets supplemented with crystalline Lys and DL-Met, and treatment groups had basal diets supplemented with encapsulated Lys and DL-Met at the levels of 80% and 60% of control diets ( 80CLM , 60CLM ), respectively. The growth performance, intestinal development, and transcription of AA transporters were determined. In experiment 2, 24 broiler chickens were subjected to the same treatments as in experiment 1. The plasma concentrations of free AAs were measured 0, 2, 4, and 6 h after feeding. The results showed that 80CLM treatment had no significant influence on production performance, carcass characteristics, and plasma free AAs content during the experiment compared with the control group ( P > 0.05). In addition, the 80CLM group moderately enhanced gut morphology development and increased AAs' absorption capacity. However, broilers fed the 60CLM diet had lower production performance and breast muscle weight than the control group ( P < 0.05), but increased villi height and B 0 AT mRNA expression level ( P < 0.05). At h 4 after feeding, the 60CLM broilers exhibited higher concentration of Ala, Cys, and total dispensable AAs than the control group ( P < 0.05). In conclusion, the result suggests that the supplemental levels of crystalline Lys and DL-Met can be effectively saved approximately for 20% by using the encapsulated form in broilers, with improvements to AAs utilization efficiency, while posing no detrimental effects on production performance. Encapsulated Lys and DL-Met would have greater potential for application when replacing crystalline AAs in broiler chickens.
研究领域:
动物营养学
·
家禽科学
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产品关联
DOI
PubMed
高影响因子
ORGANIC PROCESS RESEARCH & DEVELOPMENT
·
2022
·
IF 3.858
Jianjiong Li,Shanshan Yu,Yingang Wang,Peiyuan Yao,Qiaqing Wu,Dunming Zhu
(S)-2-Aminobutane, d-alanine, and d-homoalanine are important intermediates for the production of various active pharmaceutical ingredients and food additives. The preparation of these small chiral amine or amino acids with high water solubility still demands searching for efficient methods. In this work, we identified an ω-transaminase (ω-TA) from Sinirhodobacter hungdaonensis (ShdTA) that catalyzed the kinetic resolution of racemic 2-aminobutane at a concentration of 800 mM using pyruvate as the amino acceptor, leading to the simultaneous isolation of enantiopure (S)-2-aminobutane and d-alanine in 46% and 90% yield, respectively. In addition, (S)-2-aminobutane (98% ee) and d-homoalanine (99% ee) were isolated in 45% and 93% yield, respectively, in the kinetic resolution of racemic 2-aminobutane at a concentration of 400 mM coupled with deamination of l-threonine by threonine deaminase. We thus developed a biocatalytic process for the practical synthesis of these valuable small chiral amine and d-amino acids.
研究领域:
生物催化
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有机化学
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药物化学
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DOI
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2022
·
IF 4.966
Zhiyuan Meng,Jiajia Cui,Li Liu,Chunmei Yang,Xin Bao,Jianjun Wang,Xiaojun Chen
Chlorantraniliprole (CAP), a representative bisamide insecticide, is widely used in rice fields around the world, posing potential toxicity risks to aquatic organisms . In this study, we examined the effects of exposure to CAP on growth and metabolic phenotype of zebrafish ( Danio rerio ) and oxidative stress and apoptosis in the liver of zebrafish ( Danio rerio ). First, we identified that CAP had a low bioaccumulation in zebrafish. Subsequently, growth phenotype analysis revealed that CAP could significantly increase liver weight and liver index in zebrafish. In addition, we found that CAP exposure could cause significant changes in indicators of oxidative stress, resulting in a significant increase in the content of malondialdehyde (MDA), causing oxidative stress in the liver of zebrafish. Meanwhile, the expression levels of apoptosis-related genes were also significantly changed and apoptosis was promoted in the liver of zebrafish with CAP exposure. Importantly, the results of metabolomics analysis shown that CAP exposure could significantly disrupt the metabolic phenotype of zebrafish, interfering with multiple metabolic pathways, mainly including valine , leucine and isoleucine biosynthesis and degradation, alanine , aspartate and glutamate metabolism and d -glutamine and D-glutamate metabolism. Last but not least, correlation analysis identified strong links between changes in liver function involving oxidative stress and apoptosis and changes in metabolic phenotype of zebrafish following CAP exposure. In brief, these results indicate that potential environmental risks of CAP to aquatic organisms should receive more attention.
研究领域:
环境毒理学
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水生生物学
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代谢组学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2020
·Vol. 56
·期 1
·441-451
·
IF 2.773
Geng Yang,Rui Wang,Jing-Rong Gao,Debao Niu,Jian Li,Qing-Hui Wen,Xin-An Zeng
Summary The effect of moderate pulsed electric fields (MPEF) strength on autolysis of Saccharomyces cerevisiae was evaluated in this study. After exposure to MPEF with intensity of 7 kV cm −1 for 4 ms, the integrity of the cell wall was obviously destroyed and the inactivation of S. cerevisiae reached 99.43%. During the subsequent 42-h autolysis process, the release of free α-amino nitrogen of MPEF-treated cells, as well as extracellular protease activity, was significantly ( P < 0.05) higher than that of untreated cells. Moreover, exposure to 7 kV cm −1 led to an increase of the total amino acid of 149.36%. In particular, the content of aspartic acid and glutamic acid which are important umami flavour precursors increased by 232.55% and 209.40%, respectively. These results indicate that MPEF will be an effective method to accelerate autolysis of S. cerevisiae for obtaining high-quality yeast extract as flavour enhancers and nutrition supplements.
研究领域:
食品科学
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微生物学
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DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2024
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IF 5.6
Lu Wang,Limei Xiao,Shoujing Zheng,Jie Pang,Jiebo Chen
A better understanding of amino acids (AA) characteristics is beneficial for breeding, processing and storage of sugarcane. The differences and classification in AA of 10 different sugarcane varieties (S1-S10) were investigated in this study. Varieties affected components and concentrations of free AA ( p ＜ 0.05) significantly. The total AA of sugarcanes ranged from 51.44 to 781.61 mg/L, while the content of essential amino acids (EAA) was in the range of 0.72–60.98 mg/L. Glu (glutamic acid) was the most abundant free amino acids in all varieties of sugarcane except S4. Even though S6 had the highest concentration of medicinal AA (781.61 mg/L), the highest proportion of medicinal AA in total AA was found in S5 (81.76%). S6 got the highest comprehensive score in amino acids based on the results of Principal Component Analysis (PCA). The cluster analysis roughly divided the sugarcanes into 2 groups. Group Ⅰ including S1, S6, S2 and S5 got positive scores in PCA might had great edible and medicinal development and utilization value.
研究领域:
食品科学
·
植物学
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产品关联
DOI
高影响因子
Asian Journal of Organic Chemistry
·
2017
·Vol. 6
·期 10
·1385-1389
·
IF 2.788
Kui Wang,Jian-Hua Cui,Si-Yang Xing,Xiao-Wei Ren
Graphical I recall you ! Highly selective recognition of acidic amino acids, such as glutamic acid and aspartic acid, by a positively charged calixpyridinium is reported. Highly selective recognition of acidic amino acids, such as glutamic acid and aspartic acid, by a positively charged calixpyridinium is reported. The complexation behavior of the calixpyridinium host towards a dipeptide containing the glutamic acid residue and a glutamic acid metabolite is also described.
研究领域:
生物化学
·
分子识别
·
有机化学
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产品关联
DOI
高影响因子
AMINO ACIDS
·
2021
·Vol. 54
·期 1
·123-135
·
IF 3.52
Liang Xiaolei,Liu Kexin,Zhao Ping,Zhou Jingjing,Zhang Fangfang,He Yuhang,Zhang Hanru,Fareed Muhammad Subaan,Lu Yaqi,Xu Yue,Zhang Zhewen,Yan Wenjin,Wang Kairong
Due to the limited effects of conventional antibiotics on the increasing emergence of drug-resistant bacteria and fungi, novel antimicrobial agents were urgently needed to alleviate this phenomenon. Nowadays, antimicrobial peptides are believed to be a promising candidate for a new generation of antimicrobial drugs. Antimicrobial peptide polybia-MPII (MPII) was first isolated from the venom of the social wasp Polybia paulista with a broad spectrum of antimicrobial activity. In the present study, the counterparts and mimics of cationic amino acids of Lys, such as Arg, His, Orn, Dab and Dap were employed to substitute Lys in the sequence of MPII. The effects of the incorporation of these amino acids on its antimicrobial activity, hemolytic activity, cytotoxicity, enzyme stability and therapeutic potential were explored. Our results showed that although the incorporation of Arg could improve its antimicrobial activity, there is no improvement in enzyme stability. The incorporation of His makes MPII exert its antimicrobial activity in a pH-dependent manner. Notably, incorporating Dap could effectively decrease its hemolytic activity and cytotoxicity and enhance its enzyme stability against trypsin. In conclusion, this study would provide an effective strategy to improve the bioavailability and metabolic stability of AMPs while decrease their hemolytic activity and cytotoxicity.
研究领域:
微生物学
·
药学
·
生物化学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Haiyan Fu,Ou Hu,Yao Fan,Ying Hu,Jiahao Huang,Zhao Wang,Yuanbin She
Interests in the recognition of chiral amino acids have grown tremendously in recent years, particularly in the fields of food analysis and pharmaceutical studies. Herein, an innovative “on-off-on” fluorescent assay has been developed for the sensitive and selective determination of chiral Proline (Pro), Lysine (Lys), and Serine (Ser). This detection platform is constructed by exploiting the fluorescence quenching ability of chiral self-assembly zinc 5, 10, 15, 20-tetra(4-pyridyl)-21 H -23 H -porphine (ZnTPyP) to CdTe quantum dots (CdTe QDs). CdTe QDs can act as signal switches whose fluorescence signals can effectively quenched by chiral nano ZnTPyP and then restored in the presence of enantiomer amino acids. The coupling use of CdTe QDs and chiral nano ZnTPyP also permits quantitative analysis of d -Pro, d -Lys, l -Ser with detection limits of 4.46 × 10 −10 mol L −1 , 7.13 × 10 −11 mol L −1 , and 3.35 × 10 −11 mol L −1 , respectively. The approach is also suitable for the effective detection of the three amino acids in complex biological matrices. This rapid, robust and reliable strategy for recognizing enantiomer amino acids has not been reported. The proposed method can be a powerful and valuable technology in the areas of drug discovery and food chemistry.
研究领域:
分析化学
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生物技术
·
药学
·
食品科学
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Pengxu Wang,Qing Liu,Wenliang Fu,Chao Zhang,Guiling Cai,Weiwei Xing,Hongliang Dai,Hongya Geng,Donggang Xu
Marine mussels have evolved the secretion of diversified mussel foot proteins to firmly attach to wet, salt-crusty, corroded, and fouled surfaces. Biomimicry of this robust wet adhesion via translating sticky biological molecules is still at an impasse mainly due to the lack of available materials and methodology to fully validate the underline mechanisms of adhesion and cohesion. Increasing sources of evidence have shown that the pair structure of protonated 3,4-dihydroxyphenylalanine (DOPA) and lysine contributes to the adhesion on substrates. However, it is still an uncultivated field to study the roles of those commonly distributed lysine and non-lysine pair structures in the adhesion to different surfaces, while remaining the intermolecular cohesion strength of the pair structures. Herein, the adhesive and cohesive properties of precisely designed single-molecule domains were systematically analyzed using single-molecule force spectroscopy after surface modification of probe tips. It was revealed a record-high adhesion force of ∼ 1000 pN for DOPA-lysine on cation-rich substrates (mica) and tyrosine-Lys on cation-deficient substrates (hydrophilic modified silicon nitride, Si 3 N 4 (OH)) due to an identified hydrophobic force. DOPA-phenylalanine and DOPA-tryptophan pairs were found to show versatile strong adhesion to both cation-rich and -deficient substrates, spanning from 750 pN to 2250 pN. Furthermore, our results revealed that Phe-Lys had an extraordinarily robust cohesion strength, up to 12,000 pN due to cation-π interactions. This study broadens our view of peptides adhesion, provides single molecule proofs toward understanding the mechanism of pair structures in natural adhesive proteins, as well as the design principles of the artificial adhesive layer.
研究领域:
材料科学
·
生物物理学
·
仿生学
·
粘附科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）