INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
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2021
·Vol. 56
·期 12
·6591-6600
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IF 3.713
Jiali Yang,Tengfei Wang,Jianbing Di,Yaping Liu,Xiaoling Hao,Yu Wang
Summary Although several studies have shown the ability of l -glutamate (glutamate) to mitigate the stress imposed by pathogens, the underlying mechanism is still in its infancy. To gain further knowledge, this study focussed on the effect of glutamate on primary nitrogen and carbon metabolisms during apple- Penicillium expansum interaction. The obtained result showed that glutamate could effectively restrict blue mould rot development in apples, but had no direct impact on fungal growth in vitro . The application of glutamate increased the level of nitrogen in apples, resulting in a disrupted balance of carbon and nitrogen. Consistently, the key enzymes glutamine synthetase (GS) and glutamate synthase (GOGAT) involved in the GS/GOGAT cycle, and the deaminating activity of glutamate dehydrogenase (GDH), forming 2-oxoglutarate and ammonium from glutamate, were promptly stimulated by glutamate. Interestingly, glutamate led to considerable consumption of the tricarboxylic acid (TCA) cycle intermediates, such as isocitric acid and citric acid, accompanied by the enhancement of malate dehydrogenase and succinate dehydrogenase activities. Collectively, exogenous application of glutamate might confer blue mould resistance in apples, at least in part, by redirecting host’s primary nitrogen and carbon metabolisms, such as the activation of the GS/GOGAT cycle, deaminating activity of GDH and the TCA cycle.
研究领域:
植物病理学
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食品科学
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产品关联
DOI
高影响因子
ENVIRONMENTAL RESEARCH
·
2024
·
IF 7.7
Xingyao Ye,Xiaojun Niu,Ling Li,Mengyu Lv,Dongqing Zhang,Deye Chen,Yu Line,Zhiquan Yang
N-(1,3-Dimethylbutyl)-N′-phenyl-p-phenylenediamine (6PPD) is an antioxidant commonly used in tire manufacturing, and its release into the environment has significantly increased due to rapid urbanization. When subjected to ozonation, 6PPD converts into the harmful pollutant 6PPD quinone (6PPDQ). These substances enter wastewater treatment plants (WWTPs) via stormwater runoff and pipelines, posing significant risks to the functional microorganisms. Anammox, a strictly controlled and sensitive microbial nitrogen removal process, is especially susceptible to the effects of the pollutants. This study investigates the comprehensive impact of 6PPD-Q and 6PPD on anammox communities based on characterization analysis and metagenomics. At environmental concentrations, 6PPD-Q at 200 ng/L-1000 ng/L led to the disintegration of anammox granules. Extended exposure to both 6PPD-Q and 6PPD significantly reduces the population of anammox bacteria (AnAOB). By utilizing organic matter from dead cells and incoming carbonate as a carbon source, the system evolved into a nitrogen metabolism network primarily focused on denitrification and dissimilatory nitrate reduction to ammonium (DNRA). This transformation was accompanied by a reshuffling of the microbial community and associated genes, resulting in an accumulation of NH 4 + -N. These findings underscore the toxicity of 6PPD-Q and 6PPD to anammox and stress the importance of incorporating 6PPD into regulatory and preventive strategies.
研究领域:
环境科学
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微生物学
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DOI
PubMed
高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2025
·Vol. 41
·期 2
·358-366
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IF 3
Dong Jiaxin,Su Rui,Wu Xiaokang,Dong Deming,Wang Yining,Huang Chenni,Qiu Tao,Gu Yu,Hua Xiuyi,Liang Dapeng
The study of the metabolic toxicity of perfluorooctanoic acid (PFOA) is of great importance in assessing its potential impact on aquatic organisms and the aquatic environment, and provides a fundamental scientific basis for environmental protection and sustainable development. In this study, an electrospray ionization (ESI) source capable of direct analysis of solution samples was combined with a high-resolution Orbitrap Fusion Tribrid mass spectrometer to investigate the metabolic perturbations induced by different concentrations of perfluorooctanoic acid (PFOA) and their correlations in skin mucus and liver tissue of tilapia. Seven common metabolites were found in mucus and liver, and nitrogen metabolism pathways were disturbed in all of them. The up-regulation of glutamate in mucus may reflect an increased demand for amino acids and energy, whereas the down-regulation of glutamate in the liver may lead to dysregulation of nitrogen metabolism, protein synthesis and amino acid metabolism. Enrichment analyses suggest a dysregulation of nitrogen metabolism pathways, which may lead to impaired maintenance of tissue function and dysregulated energy metabolism in tilapia.
研究领域:
环境科学
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毒理学
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代谢组学
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产品关联
DOI
高影响因子
AQUATIC TOXICOLOGY
·
2023
·
IF 4.5
Yaxin Guo,Xiating Zhao,Zongcheng Yao,Zongyao Qian,Yuting Wang,Qiming Xian
Dissolved free amino acids are a significant component of dissolved organic nitrogen (DON) in natural waterbodies. The effects of four amino acids (glutamic acid, phenylalanine, leucine, and arginine) on the growth and microcystins (MCs) production of Microcystis aeruginosa were studied in batch culture. The profiles of five MCs variants and the expression levels of target genes involved in MCs biosynthesis and nitrogen metabolism were measured. When amino acids were used as the sole nitrogen source instead of nitrate at different levels (0.5, 2.0 and 8.0 mg/L based on N) in BG-11 medium, algal cell growth and intracellular MCs quotas were inhibited slightly by the treatments with glutamic acid and arginine. The treatments with phenylalanine and leucine, on the other hand, had a strong inhibitory effect on algal cell growth and MCs production. Moreover, the concentrations of Chlorophyll a , phycocyanin and allophycocyanin in cells cultured in glutamic acid, leucine and phenylalanine were lower than those in the control group with nitrate as nitrogen source. The existence of leucine or phenylalanine can lead to a significant increase in the relative abundance of MCs variants structured with the corresponding amino acids. The expression of microcystin-producing gene mcyD was downregulated while the gene pipX associated with nitrogen metabolism was upregulated during the cultivation of M. aeruginosa with amino acids as sole nitrogen source. M. aeruginosa undergoes significant alterations due to exogenous amino acids and exhibits advanced strategies for MCs production.
研究领域:
环境科学
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微生物学
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毒理学
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产品关联
DOI
PubMed
高影响因子
Toxics
·
2024
·Vol. 12
·期 5
·353
·
IF 3.9
Yuqi Zhu,Yili Zhang,Hui Chen,Lisha Zhang,Chensi Shen
The increasing concern over climate change has spurred significant interest in exploring the potential of microalgae for wastewater treatment. Among the various types of industrial wastewaters, high-salinity NH4+-N wastewater stands out as a common challenge. Investigating microalgae’s resilience to NH4+-N under high-salinity conditions and their efficacy in NH4+-N utilization is crucial for advancing industrial wastewater microalgae treatment technologies. This study evaluated the effectiveness of employing nitrogen-efficient microalgae, specificallyOocystis lacustris, for NH4+-N removal from saline wastewater. The results revealedOocystis lacustris’s tolerance to a Na2SO4concentration of 5 g/L. When the Na2SO4concentration reached 10 g/L, the growth inhibition experienced byOocystis lacustrisbegan to decrease on the 6th day of cultivation, with significant alleviation observed by the 7th day. Additionally, the toxic mechanism of saline NH4+-N wastewater onOocystis lacustriswas analyzed through various parameters, including chlorophyll-a, soluble protein, oxidative stress indicators, key nitrogen metabolism enzymes, and microscopic observations of algal cells. The results demonstrated that when theOocystis lacustriswas in the stationary growth phase with an initial density of 2 × 107cells/L, NH4+-N concentrations of 1, 5, and 10 mg/L achieved almost 100% removal of the microalgae on the 1st, 2nd, and 4th days of treatment, respectively. On the other hand, saline NH4+-N wastewater minimally impacted photosynthesis, protein synthesis, and antioxidant systems within algal cells. Additionally, NH4+-N within the cells was assimilated into glutamic acid through glutamate dehydrogenase-mediated pathways besides the conventional pathway involving NH4+-N conversion into glutamine and assimilation amino acids.
研究领域:
环境科学
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微生物学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2022
·Vol. 29
·期 45
·67837-67855
·
IF 5.19
Qing Bowen,Jiang Ye,Chen Yongjian,Chen Jiale,Xie Huijia,Mo Zhaowen
Nitrogen (N) modulates plant growth, but its impact on the early growth and physio-biochemical characteristics of rice under cadmium (Cd) and multiwall carbon nanotubes (MWCNTs) toxicity has received little attention. In this study, a hydroponic experiment was conducted on two fragrant rice cultivars, e.g., Xiangyaxiangzhan (XYXZ) and Yuxiangyouzhan (YXYZ), grown under two N levels (N and 1/4 N) and various Cd and MWCNTs treatments (CK: without CdCl 2 or MWCNTs; Cd: 100 μmol L −1 CdCl 2 ; MWCNTs: 100 mg L −1 MWCNTs; and Cd-MWCNTs: 100 μmol L −1 CdCl 2 + 100 mg L −1 MWCNTs). Results showed that when compared to CK, the total dry weight of the Cd and MWCNTs treatments did not change under 1/4 N for both varieties, whereas Cd and Cd-MWCNTs treatments resulted in a significant reduction in total dry weight by 18.78–37.85% for XYXZ and the Cd-MWCNTs treatment resulted in a significant reduction in the total dry weight by 20.24% for YXYZ. The changes in total dry weight were linked to changes in the dry weight of the different parts of the plant. Besides, the antioxidant parameters and the enzymes involved in the nitrogen metabolism changed in different varieties and different plant parts under two N levels and various Cd and MWCNTs treatments. In addition, differences in total dry weight changes at the N levels and various Cd and MWCNTs treatments were identified between the two varieties, and the relations between total dry weight and other investigated parameters indicated that the modulation processes varied between varieties. Overall, N modulates the early growth and physio-biochemical attributes in fragrant rice seedlings under Cd, MWCNTs, and their combined toxicity.
研究领域:
植物学
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环境科学
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农业科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Animal Nutrition
·
2024
·
IF 6.1
Yufeng Liu,Cheng Liu,Shuo Zhang,Jinming Hu,Meng M. Li,Guangyong Zhao
The objectives of the experiment were to compare the effects of rumen-protected taurine (RPT) and rumen-protected methionine (RPM) on the nitrogen (N) metabolism, plasma biochemical parameters, and metabolomics in beef steers and to clarify whether taurine plays similar roles as methionine (Met) in improving the regulation of N metabolism in beef steers. Six Simmental steers aged 12 months (liveweight 325 ± 7 kg) were used as experimental animals. The experimental treatments included a basal diet, the basal diet + 70.0 g/d RPT and the basal diet + 74.2 g/d RPM. The treatments were assigned in a replicated 3 × 3 Latin square design. Each experimental period included 15 d of adaptation and 5 d of sampling. The results showed that supplementing the diet with RPT or RPM did not affect the apparent nutrient digestibility ( P > 0.05). Supplementing the diet with RPT or RPM increased the N retention ( P < 0.05) and the N utilization efficiency (NUE) ( P < 0.05) and decreased the urinary excretion of 3-Methylhistidine ( P < 0.05) and the estimated skeletal protein degradation rate ( P < 0.05). Supplementing the diet with RPT increased the plasma concentrations of taurine ( P < 0.001), cysteine ( P = 0.010), valine ( P = 0.013) and total non-essential amino acids (NEAA) ( P = 0.047) and tended to increase the plasma concentrations of essential amino acids (EAA) + NEAA ( P = 0.087), but it did not affect the plasma concentrations of total EAA ( P > 0.05). Supplementing the diet with RPM increased the plasma concentrations of methionine ( P = 0.033), lysine ( P = 0.047), cysteine ( P = 0.007), leucine ( P = 0.046), isoleucine ( P = 0.046), valine ( P = 0.034), total EAA ( P = 0.028), total NEAA ( P = 0.004) and EAA + NEAA ( P = 0.004). The plasma metabolomics profiling revealed that supplementing the diet with RPT upregulated the plasma concentrations of taurine ( P < 0.001), L-Cysteine ( P = 0.004) and some amino acid (AA) analogues ( P < 0.05) and RPM upregulated the plasma concentrations of Met ( P = 0.021), L-Isoleucine ( P = 0.036), L-Tryptophan ( P = 0.006) and some AA analogues ( P < 0.05). In conclusion, taurine has similar impacts to Met in improving the N retention and the NUE in beef steers. Taurine deficiency is one of the main reasons that the NUE of beef steers is low. Supplementation of the diet with taurine is beneficial to the N utilization in beef steers.
研究领域:
动物营养学
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畜牧学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Plant Science
·
2024
·
IF 4.1
Yu Bo,Xue Xiaomin,Nie Peixian,Lu Ninglin,Wang Laiping
Previous studies have determined that fulvic acid (FA) prevents plants from suffering from abiotic stress. However, little is known about the mechanisms for using FA to increase apple trees resistance to Cd toxicity. In order to clarify the mechanism underlying FA alleviation in Cd-induced growth inhibition in apple seedlings. Herein, we treated M9T337 seedlings to either 0 or 30 μM/L Cd together with 0 or 0.2 g/L FA and analyzed the root growth, antioxidant enzyme activities, carbon (C) assimilation, nitrogen (N) metabolism, and C and N transport. The results presented that, compared with CK (without Cd addition or FA spraying application), Cd poisoning significantly inhibited the root growth of apple seedlings. However, this Cd-induced root growth inhibition was significantly alleviated by FA spraying relative to the Cd treatment (Cd addition alone). On the one hand, the mitigation of inhibition effects was due to the reduced oxidative damage by enhancing antioxdiant enzyme (SOD, POD, and CAT) activities in leaves and roots.On the other hand, this growth advantage demonstrated compared to the Cd treatment was found to be associated with the strengthen of photosynthetic performance and the elevation of C and N metabolism enzymes activities. Meanwhile, we also found that under Cd stress condition, the distribution of C and N nutrients in apple seedlings was optimised by FA spraying application relative to the Cd treatment, according to the results of 13 C and 15 N tracing. Conclusively, our results suggested that the inhibitory effect of Cd on apple seedlings root growth was alleviated by FA through regulating antioxdiant capacities and C and N metabolism.
研究领域:
植物生理学
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环境科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Hao Jiang,Haodong Wang,Cancan Qian,Zhenlong Yang,Desong Yang,Jianghu Cui
To promote the growth and yield of crops, it is necessary to develop an effective silicon fertilizer. Herein, a new type of 2 nm silicon quantum dot (SiQD) was developed, and the phenotypic, biochemical, and metabolic responses of rice seedlings treated with SiQDs were investigated. The results indicated that the foliar application of SiQDs could significantly improve the growth of rice seedlings by increasing the uptake of nutrient elements and activating the antioxidative defense system. Furthermore, metabolomics revealed that the supply of SiQDs could significantly up-regulate several antioxidative metabolites (oxalic acid, maleic acid, glycine, lysine, and proline) by reprogramming the nitrogen- and carbon-related biological pathways. The findings provide a new strategy for developing an effective and promising quantum fertilizer in agriculture.
研究领域:
植物学
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农业科学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2022
·Vol. 106
·期 19
·6551-6566
·
IF 5.56
Khan Sabir,Xu Xueqi,Song Jialei,Wu Panpan,Liu Xiaobin,Liu Jing,Chen Ketao,Xu Zhenyue,Wu Hang,Zhang Buchang
Abstract TetR family transcriptional regulators (TFRs) are widespread in actinomycetes, which exhibit diverse regulatory modes in antibiotic biosynthesis. Nitrogen regulators play vital roles in modulation of primary and secondary metabolism. However, crosstalk between TFR and nitrogen regulator has rarely been reported in actinomycetes. Herein, we demonstrated that a novel TFR, SACE_4839, was negatively correlated with erythromycin yield in Saccharopolyspora erythraea A226. SACE_4839 indirectly suppressed erythromycin synthetic gene eryAI and resistance gene ermE and directly inhibited its adjacent gene SACE_4838 encoding a homologue of nitrogen metabolite repression (NMR) regulator NmrA (herein named NmrR). The SACE_4839-binding sites within SACE_4839-nmrR intergenic region were identified. NmrR positively controlled erythromycin biosynthesis by indirectly stimulating eryAI and ermE and directly repressing SACE_4839 . NmrR was found to affect growth viability under the nitrogen source supply. Furthermore, NmrR directly repressed glutamine and glutamate utilization-related genes SACE_1623 , SACE_5070 and SACE_5979 but activated nitrate utilization-associated genes SACE_1163 , SACE_4070 and SACE_4912 as well as nitrite utilization-associated genes SACE_1476 and SACE_4514 . This is the first reported NmrA homolog for modulating antibiotic biosynthesis and nitrogen metabolism in actinomycetes. Moreover, combinatorial engineering of SACE_4839 and nmrR in the high-yield S. erythraea WB resulted in a 68.8% increase in erythromycin A production. This investigation deepens the understanding of complicated regulatory network for erythromycin biosynthesis. Key points
SACE_4839 and NmrR had opposite contributions to erythromycin biosynthesis.
NmrR was first identified as a homolog of another nitrogen regulator NmrA.
Cross regulation between SACE_4839 and NmrR was revealed.
研究领域:
微生物学
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分子生物学
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生物技术
✓ 已验证
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2024
·
IF 9.7
Shuyan Yin,Yi-Xuan Wang,Cheng Hou,Jing Wang,Jing Xu,Xinbai Jiang,Dan Chen,Yang Mu,Jinyou Shen
Anammox coupled partial S 0 -driven autotrophic denitrification (PS 0 AD) technology represents an innovative approach for removing nitrogen from wastewater. The research highlighted the crucial role of biofilm on sulfur particles in the nitrogen removal process. Further analysis revealed that sulfur-oxidizing bacteria (SOB) are primarily distributed in the inner layer of the biofilm, while anammox bacteria (AnAOB) are relatively evenly distributed in inner and outer layers, with Thiobacillus and Candidatus Brocadia being the dominant species, respectively. Except for anammox and PS 0 AD processes, 15 N isotope labeling tests determined that sulfur reshaped nitrogen metabolism pathways, providing solid evidence for the occurrence of sulfammox process. SOB and AnAOB collaborate in nitrogen and sulfur conversion, with SOB-drived PS 0 AD processes reducing nitrate to nitrite for AnAOB to remove ammonia. Conversely, the nitrate produced from anammox process can be reused by SOB. Metagenomic analyses verified that SOB drove the PS 0 AD process through encoding soxBYZ gene, while AnAOB might play an important role in simultaneously driving the anammox and sulfammox processes. These findings underscore the importance of biofilm and clarify the nitrogen-sulfur cycle mechanisms within the coupled system.
研究领域:
环境微生物学
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废水处理工程
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分子生态学
✓ 已验证
DOI
高影响因子
Animal Nutrition
·
2025
·
IF 7.5
Manman Fan,Jinming Hu,Cheng Liu,Shuo Zhang,Yufeng Liu,Guangyong Zhao
Taurine is a sulfur-containing nonproteinogenic amino acid. Recent studies have shown that taurine can improve rumen microbial crude protein (MCP) synthesis. This experiment aimed to investigate the action mechanism of taurine on rumen MCP synthesis and nitrogen (N) metabolism in beef steers using sodium sulfate (Na 2 SO 4 ) as a contrast. Six steers (bodyweight 506 ± 17 kg) were assigned to three experimental groups including a basal diet (control), a basal diet supplemented with 45 g taurine/d or 50 g Na 2 SO 4 /d, and were allocated in a replicated 3 × 3 Latin square design. The amounts of sulfur from taurine and Na 2 SO 4 were equal (11.38 g/d). The results showed that, compared with the control group, both taurine and Na 2 SO 4 increased ruminal production of MCP ( P < 0.05) by 37.50% and 29.17%, respectively, and increased the concentration of sulfide (S 2- ) ( P < 0.001). Both taurine and Na 2 SO 4 increased the digestibility of neutral detergent fiber ( P < 0.05). Taurine tended to increase ( P = 0.087) while Na 2 SO 4 decreased plasma urea concentration ( P = 0.049), with the taurine group exhibiting higher plasma urea concentrations than the Na 2 SO 4 group ( P = 0.003). Taurine did not affect urinary urea excretion ( P = 0.246) whereas Na 2 SO 4 decreased urinary urea excretion compared with the control group ( P = 0.002). Compared with the control group, both taurine and Na 2 SO 4 increased urinary allantoin excretion ( P < 0.05), total purine derivatives excretion ( P < 0.05), and the estimated rumen microbial N flow ( P < 0.05). The urinary urea excretion of the taurine group was higher than the Na 2 SO 4 group ( P = 0.019). Compared with the control group, taurine did not affect N excretion, N retention (NR) or N utilization efficiency (NUE) ( P > 0.10), but Na 2 SO 4 decreased urinary N excretion ( P = 0.018) and total N excretion ( P = 0.024), and increased NR ( P = 0.024) and NUE ( P = 0.022). No differences were found in NR and NUE between the taurine and Na 2 SO 4 groups ( P > 0.10). Taurine improved ruminal MCP synthesis by enriching the pathways associated with sulfur and amino acid metabolism while Na 2 SO 4 improved ruminal MCP synthesis by enriching pathways related to nucleotide and purine metabolism. In summary, both taurine and Na 2 SO 4 improved ruminal MCP synthesis by modulating different pathways. Taurine was less effective in decreasing total N excretion than Na 2 SO 4 but no differences in NR and NUE were found between the two groups of beef steers.
研究领域:
动物营养学
·
畜牧学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）