INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 14
·7574
·
IF 6.208
Weizeng Lv,Xue He,Haojuan Guo,Haibin Lan,Yanqing Jiao,Le Li,Yanhao Lian,Zhiqiang Wang,Zeyu Xin,Yongzhe Ren,Tongbao Lin
Excessive input of nitrogen fertilizer not only causes a great waste of resources but brings about a series of ecological and environmental problems. AlthoughSmall Auxin Up-regulatedRNAs(SAURs) participate in diverse biological processes, the function ofSAURsin the nitrogen starvation response has not been well-studied. Here, we identified 308TaSAURs in wheat and divided them into 10 subfamilies. The promoter regions of mostTaSAURscontain hormone responsive elements, and their expression levels change under the treatment of different hormones, such as IAA, MeJA, and ABA. Interestingly, overexpression of one of theTaSAURfamily members, a nitrogen starvation responsive gene,TaSAUR66-5B, can promote the growth ofArabidopsisand wheat roots. In addition, overexpression ofTaSAUR66-5BinArabidopsisup-regulates the expression levels of auxin biosynthesis related genes, suggesting that overexpressionTaSAUR66-5Bmay promote root growth by increasing the biosynthesis of auxin. Furthermore, overexpression ofTaSAUR66-5Bin wheat can increase the biomass and grain yields of transgenic plants, as well as the nitrogen concentration and accumulation of both shoots and grains, especially under low nitrogen conditions. This study provides important genomic information of theTaSAURgene family and lays a foundation for elucidating the functions ofTaSAURsin improving nitrogen utilization efficiency in wheat.Keywords:TaSAURgene family;TaSAUR66-5B;root;nitrogen utilization efficiency
研究领域:
植物学
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遗传学
·
分子生物学
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DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2021
·Vol. 139
·期 2
·51484
·
IF 3.125
Xin Qi,Yu Guo,Qianlin Chen,Xiaohan Zhao,Xianquan Ao
Slow-release fertilizers (MSRFs) are effective in improving fertilizer utilization and reducing environmental fertilizer pollution. In this study, polyvinyl alcohol (PVA) was modified with kitchen waste oil (KWO) and grafted with urea to prepare a urea MSRF. FTIR, Scanning electron microscopy, X-ray diffraction and water contact angle were used to study the MSRF structure and properties. The effects of the nitrogen content on its properties were also investigated through release performance and degradation behavior evaluation and plant experiments. The results showed that the oil-based polyalcohol-modified PVA (OPMPVA) solution prepared from KWO had good compatibility with the urea-modified compound hydroxylurea, and the prepared MSRF had good hydrophobicity, degradation and slow-release properties. The nutrient release period reached more than 20 days, and the degradation rate after 30 days reached 10.2%. Plant experiments showed that the MSRF prepared in this research has good application potential, and the utilization of nitrogen nutrients was improved.
研究领域:
农业科学
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环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2020
·
IF 6.551
Qingnan Chu,Sheng Xu,Lihong Xue,Yang Liu,Yanfang Feng,Shan Yu,Linzhang Yang,Baoshan Xing
Clay-hydrochar composites (CHCs) are of great significance in ammonium (NH 4 + ) adsorption and have the potential to be applied to paddy fields to prevent ammonia (NH 3 ) volatilization. In this study, three CHCs were produced by infusing different clays to poplar-sawdust-derived hydrochar , including a bentonite hydrochar composite (BTHC), montmorillonite hydrochar composite (MTHC), and kaolinite hydrochar composite (KTHC). These three CHCs were applied to a paddy soil column system growing rice. The temporal variations in NH 3 volatilization and NH 4 + loss in floodwater were monitored after three fertilization dates. The results showed that among the three CHCs, only the BTHC significantly reduced cumulative NH 3 volatilization (by 41.8%), compared to that of the unamended control (without addition of hydrochar or clay-hydrochar-composite). In the unamended control, NH 3 volatilization loss accounted for 31.4% of the applied N fertilizer; with the BTHC amendment, NH 3 volatilization loss accounted for 17.4% of the applied N fertilizer. The reduced N loss via the BTHC amendment resulted in an increased N supply and further improved the N use efficiency and yield by 37.36% and 18.8% compared to that of the control, respectively. The inhibited NH 3 volatilization was mainly attributed to the increased soil NH 4 + retention as a result of BTHC's larger pore volume and specific surface area. In addition, the BTHC treatment significantly reduced the abundance of archaeal amoA genes ( AOA ), which possibly inhibited nitrification and increased soil NH 4 + retention. This study, for the first time, screened BTHC as an excellent material for mitigating NH 3 volatilization from paddy fields. The reduced NH 3 volatilization loss might contribute to increased soil N retention and plant N use efficiency.
研究领域:
农业科学
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环境科学
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DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2021
·Vol. 139
·期 12
·51810
·
IF 3.125
Songling Chen,Yanyu Han,Chunyu Chen,Hongdou Liu,Hongtao Zou
Nearly 100 million tons of spent mushroom substrates (SMS) are discarded annually in China, which causes a series of environmental pollutants. To improve the nitrogen (N) use efficiency of and to shift dependence away from petroleum-based products, a novel bio-based epoxy coated urea was successfully developed. The bio-based epoxy coating was synthesized from the liquefied SMS and bisphenol-A diglycidyl ether. Subsequently, both nano-SiO 2 and organosilicon were employed to modify the bio-based epoxy and increase its hydrophobicity. Water absorption, ammonium adsorption, and water contact angles of the bio-based epoxy coatings were investigated. The nutrient release behaviors of the bio-based epoxy coated urea in water were also investigated and the modification mechanisms were explored by Fourier transform infrared, scanning electron microscopy–energy-dispersive X-ray spectroscopy and thermogravimetric analysis. The results revealed that the hydrophobic ability of the nano-SiO 2 and organosilicon dual modified bio-based epoxy coated urea coating was significantly enhanced and that its nutrient release longevity was increased to 56 days. Therefore, this work demonstrated that bio-based epoxy coated urea with superior release characteristics exhibited great potential in modern agricultural applications.
研究领域:
环境科学
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农业科学
✓ 已验证
产品关联
DOI
高影响因子
ChemSusChem
·
2017
·Vol. 10
·期 9
·2068-2079
·
IF 7.226
Linglin Zhou,Pan Zhao,Yu Chi,Dongfang Wang,Pan Wang,Ning Liu,Dongqing Cai,Zhengyan Wu,Naiqin Zhong
Graphical Let it grow : A network-structured nanocomposite is fabricated and used as an excellent fertilizer synergist (FS), which could effectively inhibit the hydrolysis, reduce the loss, and enhance the utilization efficiency of nitrogen and thus decrease nonpoint agricultural pollution. The FS, which has high biosafety, exerted positive effects on the expression of nitrogen-uptake-related genes, ion flux in maize roots, crop growth, microbial communities, and organic matter in soil. Urea tends to be hydrolyzed by urease and then migrate into the environment, which results in a low utilization efficiency and severe environmental contamination. To solve this problem, a network-structured nanocomposite (sodium humate–attapulgite–polyacrylamide) was fabricated and used as an excellent fertilizer synergist (FS) that could effectively inhibit the hydrolysis, reduce the loss, and enhance the utilization efficiency of nitrogen. Additionally, the FS exerted significant positive effects on the expression of several nitrogen-uptake-related genes, ion flux in maize roots, the growth of crops, and the organic matter in soil. The FS could modify the microbial community in the soil and increase the number of bacteria involved in nitrogen metabolism, organic matter degradation, the iron cycle, and photosynthesis. Importantly, this technology displayed a high biosafety and has a great potential to reduce nonpoint agricultural pollution. Therefore, this work provides a promising approach to manage nitrogen and to promote the sustainable development of agriculture and the environment.
研究领域:
农业科学
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环境科学
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植物学
✓ 已验证
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
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2023
·
IF 9.8
Wangjing Zhai,Wenqi Jiang,Qiqi Guo,Zhixuan Wang,Donghui Liu,Zhiqiang Zhou,Peng Wang
Due to sewage irrigation, manure fertilizer application or other agricultural activities, antibiotics have been introduced into farmland as an emerging contaminant , existing with other agrochemicals . However, the potential influences of antibiotics on the efficiency of agrochemicals and crops health are still unclear. In this work, the effect of antibiotics on fertilization efficiency and pea yield was evaluated, and the mechanism was explored in view of soil microbiome. Nitrogen utilization and pea yield were decreased by antibiotics. In specific, the weight of seeds decreased 9.5 % by 5 mg/kg antibiotics and decreased 25.1 % by 50 mg/kg antibiotics. For N nutrient in pea, antibiotics resulted in 62.5 %–63.7 % decrease in amino acid content and 8.3 %–35.3 % decrease in inorganic-N content. Further research showed that antibiotics interfered with N cycle in soil, inhibiting urea decomposition and denitrification process by reducing function genes ure C, nir K and nor B in soil, thus decreasing N availability. Meanwhile, antibiotics destroyed the enzyme function in N assimilation. This work evaluated the environmental risk of antibiotics from fertilization efficiency and N utilization in crop. Antibiotics could not only affect N cycle, limiting the decomposition of N fertilizer, but also affect N utilization in plants, thus affecting the yield and even the quality of leguminous crops.
研究领域:
农业
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环境科学
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微生物学
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植物科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PLANT PHYSIOLOGY
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2025
·
IF 4.1
Mi Qi-Li,Lv Hong-Tao,Huang Fei-Fei,Xu Li,Huang Zi-Ying,Yang Wen-Wu,Zou Mei-Yun,Huang Hai-Tao,Zeng Wan-Li,Zhao Jia-Yin,Chen Qing-Xian,Si-Tu Yong-En,Xiang Hai-Ying,Jiang Yuan-Qi,Mai Zhi-Tong,Ding Shu-Yuan,Liu Zhan-Yu,Hu Bo-Keng,Li Liu-Hong,Li Xue-Mei,Yu Min,Wu Fei-Hua,Gao Qian
Nitrogen is a critical nutrient for plant growth and development. While numerous studies have investigated the mechanisms by which nitrate and/or ammonium regulate plant growth, little is known about whether and how amino acids regulate plant leaf development. This study demonstrates that tobacco plants with altered expression levels of an amino acid transporter (LYSINE HISTIDINE TRANSPORTER1, NtLHT1 , Ntab0818090) exhibit significant differences in leaf morphology. Knock-out mutants exhibit elongated and narrower leaves compared to wild-type plants, whereas overexpression (OE) lines display orbicular leaves. Additionally, mutant plants exhibit decreased nitrogen use efficiency (NUE) under half MS medium and delayed development under nitrogen-depleted conditions. Moreover, overexpression lines demonstrate better performance. Although the mutant does not show significant lower level of nitrate or total amino acid content in the developing leaves, its amino acid profile, particularly glutamine (Gln), is significantly altered. Supplementation with Gln in the growth medium, rather than glutamate, can restore the morphological differences observed in mutant leaves, suggesting a pivotal role of Gln in regulating leaf shape. To further elucidate the mechanisms underlying leaf shape regulation, we analyzed endogenous hormone levels and applied exogenous hormones to these lines. Our findings suggest that amino acids transported from source organs, particularly glutamine (Gln), play a key role in controlling leaf development and morphology through the modulation of multiple phytohormones, such as gibberellic acid (GA).
研究领域:
植物学
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遗传学
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分子生物学
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营养生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nature Plants
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2023
·
IF 18
Xie Yuanming,Lv Yuanda,Jia Letian,Zheng Lulu,Li Yonghui,Zhu Ming,Tian Mengjun,Wang Ming,Qi Weicong,Luo Long,De Gernier Hugues,Pélissier Pierre-Mathieu,Motte Hans,Lin Shaoyan,Luo Le,Xu Guohua,Beeckman Tom,Xuan Wei
Ammonium toxicity affecting plant metabolism and development is a worldwide problem impeding crop production. Remarkably, rice ( Oryza sativa L.) favours ammonium as its major nitrogen source in paddy fields. We set up a forward-genetic screen to decipher the molecular mechanisms conferring rice ammonium tolerance and identified rohan showing root hypersensitivity to ammonium due to a missense mutation in an argininosuccinate lyase (ASL)-encoding gene. ASL localizes to plastids and its expression is induced by ammonium. ASL alleviates ammonium-inhibited root elongation by converting the excessive glutamine to arginine. Consequently, arginine leads to auxin accumulation in the root meristem, thereby stimulating root elongation under high ammonium. Furthermore, we identified natural variation in the ASL allele between japonica and indica subspecies explaining their different root sensitivity towards ammonium. Finally, we show that ASL expression positively correlates with root ammonium tolerance and that nitrogen use efficiency and yield can be improved through a gain-of-function approach.
研究领域:
植物科学
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分子生物学
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农业
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Animal Nutrition
·
2025
·
IF 7.5
Zhanbo Xiong,Xiaoyin Zhang,Shiqi Zhang,Kexin Li,Yuan Gao,Ying Bu,Nan Zheng,Shengguo Zhao,Jiaqi Wang
Reducing nitrogen loss and improving nitrogen utilization efficiency in dairy cows can reduce economic costs and mitigate the environmental impact of nitrogen emissions. Red clover isoflavone, a natural compound derived from plant extract, has the advantage of biological safety. This study aimed to investigate the effects of red clover isoflavone on lactation performance and nitrogen metabolism in dairy cows. Sixty-eight Holstein cows (165 ± 21 d in milk, 710 ± 75 kg initial body weight, 2.30 ± 1.21 parities, 33.93 ± 3.81 kg/d milk yield) were chosen in a randomized complete block design. Cows were divided into 5 blocks by milk yield and randomly assigned to 4 treatments (17 cows per treatment). The experimental period lasted 84 d, during which cows were fed a basal diet supplemented with red clover extract (RCE) at 0, 2, 4, or 8 g/kg of total mixed rations (TMR) on a dry matter (DM) basis. These levels corresponded to red clover isoflavones of 0, 0.46 (0.31 g/kg formononetin and 0.15 g/kg biochanin A), 0.93 (0.67 g/kg formononetin and 0.26 g/kg biochanin A), and 1.83 g/kg (1.32 g/kg formononetin and 0.52 g/kg biochanin A), respectively. With increasing supplementation of RCE in TMR, milk yield increased quadratically by 4.65% to 9.64% ( P = 0.020) and feed efficiency improved by 6.58% to 10.53% ( P = 0.005), with the optimal dose identified as 2 g/kg. Additionally, RCE supplementation significantly increased the yield of milk components, including fat, protein, lactose, non-fat milk solid, and total milk solid ( P < 0.05), with the highest in the 2 g/kg group. Regarding nitrogen metabolism, RCE supplementation linearly increased microbial nitrogen (MN) from 782.61 to 956.41 g/d ( P = 0.003), which was accompanied by a linear increase in nitrogen utilization efficiency from 26.32% to 30.31% ( P < 0.001). In contrast, urinary total nitrogen ( P = 0.027) and milk urea nitrogen ( P < 0.001) decreased quadratically with increasing doses of RCE, reaching their minimum at 4 g/kg. Following RCE supplementation, the apparent digestibility of DM, organic matter (OM), and neutral detergent fiber (NDF) exhibited a quadratic effect ( P < 0.05), with significantly higher digestibility observed in the 2 g/kg group. Metabonomics results revealed that RCE supplementation significantly upregulated the top 12 differential metabolites. These findings suggest that RCE supplementation at levels ranging from 2 to 4 g/kg DM enhances lactation performance, milk quality, and nitrogen metabolism in dairy cows.
研究领域:
动物营养学
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乳品科学
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代谢学
·
植物化学
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环境生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
WASTE MANAGEMENT
·
2024
·
IF 7.1
Jun Zhang,Kun Yu,Menghan Yu,Xiongbo Dong,Muhammad Tariq Sarwar,Huaming Yang
Phosphogypsum (PG) presents considerable potential for agricultural applications as a secondary primary resource. However, it currently lacks environmentally friendly, economically viable, efficient, and sustainable reuse protocols. This study firstly developed a PG-based mineral slow-release fertilizer (MSRFs) by internalization and fixation of urea within the PG lattice via facet-engineering strategy. The molecular dynamics simulations demonstrated that the binding energy of urea to the (0 4 1) facet of PG surpassed that of the (0 2 1) and (0 2 0) facets, with urea's desorption energy on the (0 4 1) facet notably higher than on the (0 2 1) and (0 2 0) facets. Guided by these calculations, we selectively exposed the (0 4 1) dominant facet of PG, and then achieving complete urea fixation within the PG lattice to form urea-PG (UPG). UPG exhibited a remarkable 48-fold extension in N release longevity in solution and a 45.77% increase in N use efficiency by plants compared to conventional urea. The facet-engineering of PG enhances the internalization and fixation efficiency of urea for slow N delivery, thereby promoting nutrient uptake for plant growth. Furthermore, we elucidated the intricate interplay between urea and PG at the molecular level, revealing the involvement of hydrogen and ionic bonding. This specific bonding structure imparts exceptional thermal stability and water resistance to the urea within UPG under environmental conditions. This study has the potential to provide insights into the high-value utilization of PG and present innovative ideas for designing efficient MSRFs.
研究领域:
农业科学
·
材料科学
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环境科学
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Science
·
2022
·Vol. 9
·期 16
·2200732
·
IF 17.521
Huiling Zhou,Dongsheng Tang,Xiaoxu Kang,Haitao Yuan,Yingjie Yu,Xiaolu Xiong,Nier Wu,Fangzhou Chen,Xing Wang,Haihua Xiao,Dongsheng Zhou
Photothermal therapy based on conjugated polymers represents a promising antibacterial strategy but still possesses notable limitations. Herein, degradable pseudo conjugated polymers (PCPs) containing photothermal molecular backbones and reactive oxygen species (ROS)-sensitive thioketal bonds are designed. Triphenylphosphine (PPh 3 ) is introduced into PCPs to generate phosphonium-based PCPs (pPCPs), which further assembled with hyaluronic acid into pPCP nanoparticles (pPCP-NPs). pPCP-NPs with quaternary phosphonium cations selectively anchor on and destroy bacterial cell membranes through electrostatic action. Under 1064 nm laser irradiation, pPCP-NPs (pPCP-NPs/+L) produce near-infrared-II (NIR-II) photothermal antibacterial effect, thereby killing bacteria in a sustained manner. pPCP-NPs are readily degraded upon ROS abundant at infection sites, therefore exhibiting enough biosafety. pPCP-NPs/+L display an almost 100% bacterial inhibition rate in vitro and resultin a nearly complete recovery of bacteria-induced mouse wounds. A further metabolomics analysis denotes that pPCP-NPs/+L work in a concerted way to induce bacterial DNA damage, inhibit bacterial carbon/nitrogen utilization and amino acid/nucleotide synthesis. Taken together, degradable pPCP-NPs with both NIR-II photothermal effect and cationic phosphonium structural bacteriostasis provide a new avenue for antibiotics-alternative anti-infection therapy.
研究领域:
材料科学
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纳米技术
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生物医学工程
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药学
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微生物学
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治疗学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）