CURRENT BIOLOGY
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2024
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IF 8.1
Yun Chen,Xia Hua,Siyu Li,Jiamei Zhao,Huan Yu,Dongyao Wang,Jiqiang Yang,Lijun Liu
Rice paddy has been the main source of anthropogenic methane (CH 4 ) emissions, with significant variations among rice varieties. 2-Acetyl-1-pyrroline (2-AP) is the key component of the pleasant aroma in fragrant rice. Here, we show that fragrant rice is metabolically active in nitrogen assimilation and exhibits high levels of 2-AP and that CH 4 fluxes at the booting stage and cumulative emissions are 25.5% and 14.8% lower, respectively, in fragrant rice paddies compared with nonfragrant rice paddies. Three precursors involved in 2-AP synthesis—proline, glutamic acid, and ornithine—are identified as crucial nitrogen compounds that significantly promote CH 4 oxidation in the rhizosphere. Augmenting 2-AP synthesis, either through foliar spraying or by utilizing CRISPR-Cas9 technology to generate knockout lines of BETAINE ALDEHYDE DEHYDROGENASE 2 gene, effectively enhances CH 4 oxidation and reduces CH 4 fluxes. Our findings reveal that the 2-AP metabolic pathway coordinates the carbon/nitrogen cycle to improve nitrogen assimilation along with high 2-AP levels and mitigate CH 4 emissions in paddy ecosystems.
研究领域:
植物学
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环境科学
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遗传学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2022
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IF 7.129
Changjiang Zhao,Yuyuan Shi,Yanmei Xu,Ni Lin,Hang Dong,Lixia Bei
Bisphenol A (BPA) is becoming a potential environmental toxicity factor. However, BPA's effect and function mechanism on maize roots remain unknown. Here, we investigated characters of root growth of maize seedlings exposed to BPA for 8 d and without BPA for 3 d, and a series of indicators on reactive oxygen homeostasis and nitrogen assimilation were measured. High-dose BPA(15 and 50 mg·L −1 ) suppressed the root growth and caused increased contents of O 2 ˑ- , H 2 O 2 and MDA in maize seedling roots. The disturbed ROS homeostasis resulted from the change of antioxidant enzymes, including the increase of APX, GPX, and CAT, and decrease of SOD and POD, and a decrease of antioxidant substance GSH. Meanwhile, High-dose BPA caused a decrease in the soluble protein content, nitrate reductase (NR), glutamate dehydrogenase (GDH), and glutamine oxoglutarate aminotransferase (GOGAT) under the BPA processing phase and recovery period. The low-dose BPA（1.5 and 5 mg·L −1 ）significantly promoted root growth of maize seedlings and maintained the ROS homeostasis through antioxidant enzyme APX and GPX eliminating redundant ROS. Our results showed that BPA could cause a dual effect on the root growth of maize seedlings, that is, promotion of low-dose and inhibition of high-dose, through ROS homeostasis and nitrogen assimilation in Zea mays .
研究领域:
植物生理学
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环境毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2025
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IF 11.3
Yanyu Zhou,Fucun Liu,Meile Yuan,Xianhua Liu,Qipei Li,Hongwei Zhao
Ocean warming threatens the health of corals globally, and superimposed coastal environmental pollution can result in severe and irreversible coral bleaching. However, the responses of the coral symbiont Symbiodiniaceae to multiple stresses remain largely unknown. This study investigated the response of the coral symbiotic algae Cladocopium sp. to short-term exposure (4 days) to an environmentally relevant concentration (1 μg L −1 ) of the photosystem II (PSII) herbicide prometryn under heat stress (32 ℃) through physiological and omic analyses. These results showed that co-stress affected the photosynthetic efficiency of Cladocopium sp. negatively. Overproduction of reactive oxygen species and subsequent oxidative stress under co-stress activated distinct regulatory pathways in Cladocopium sp. Transcriptomic and proteomic analyses revealed that prometryn exacerbated heat stress-induced photosystem damage and reduced the regulatory capacity of Cladocopium sp. Moreover, co-stress disrupted energy metabolism, and further impaired nitrogen assimilation and nutrient transfer processes, potentially compromising the symbiotic potential between corals and Symbiodiniaceae. In summary, this study offers a valuable insight into understanding the molecular responses of Symbiodiniaceae to thermal and prometryn co-stress. It helps uncover the potential toxicity mechanisms induced by herbicide on coral symbionts in the context of climate change.
研究领域:
海洋生物学
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环境科学
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分子生态学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
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2017
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IF 6.32
Li Cui,Kai Yang,Guowei Zhou,Wei E. Huang,Yong-Guan Zhu
Microbe-mediated biogeochemical cycle of nitrogen is a critical process in the environment. In this study, surface-enhanced Raman spectroscopy combined with 15N stable isotope probing (SERS–15N SIP) was developed as a new, nondestructive, and robust approach to probe nitrogen assimilation by bacteria at both bulk and single-cell level, and from pure culture to environmental microbial community. Multiple distinguishable SERS band shifts were observed and displayed a linear relationship with 15N content, because of the substitution of “light” nitrogen by “heavier” 15N stable isotope. These shifts, especially in 730 cm–1 band, were highly distinguishable and universal in different bacteria, providing a robust indicator for nitrogen assimilation in bacteria. SERS–15N SIP was also demonstrated in important N2-fixing bacteria via 15N2 incubations. The same prominent shifts as that induced by 15NH4Cl were observed, indicating the applicability of SERS–15N SIP to different nitrogen sources. SERS–15N SIP was further applied to environmental microbial community via 15NH4Cl, 15NO3–, and 15N2 incubation. Bacteria- and nitrogen source-dependent activity in nitrogen assimilation were revealed in environmental microbial community, pointing to the bacterial diversity and necessity of single-cell level investigation. Finally, by mixing optimized ratio of bacteria with Ag NPs, explicit single-cell SERS–15N SIP was obtained. The nondestructive SERS–15N SIP approach will be useful not only to identify active nitrogen-assimilating cells, but also enable Raman activated cell sorting and downstream genomic analysis, which will bring in deep insights into nitrogen metabolism of environmental microorganisms.
研究领域:
环境生物学
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微生物学
✓ 已验证
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
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2025
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IF 6.2
Shuhao Li,Xin Zhong,Ji Sun,Huanwen Xu,Shengmei Zhang,Qingming Li,Chengbo Zhou
Common broad-spectrum ultraviolet (UV) filters—oxybenzone (OBZ), avobenzone (AVB), octocrylene (OCR), and octinoxate (OMC)—can accumulate in natural environment at concentrations toxic to plants, yet their mechanisms of toxicity remain unclear. In this study, pakchoi ( Brassica rapa ssp. chinensis ) was individually exposed to these UV filters, and transcriptomic analysis revealed that approximately 8,500 genes were differentially expressed, especially those related to photosynthesis, antioxidant defense, nitrogen use, and amino acid biosynthesis. All four UV filters inhibited plant growth through multiple pathways, but their toxicological profiles differed. AVB, OCR, and OMC primarily suppressed genes involved in photosynthesis, reducing light capture and carbon fixation. In contrast, OBZ exposure upregulated photosynthetic genes, likely as a compensatory mechanism. All treatments triggered oxidative stress, indicated by increased reactive oxygen species (ROS) and expression of damage biomarkers, which activated antioxidant defenses; notably, OMC markedly increased peroxidase activity, possibly reinforcing cell wall resilience. UV filter treatments also impaired nitrogen assimilation by downregulating key enzymes, leading to increased nitrate uptake, and disrupted amino acid balance, with selective increases and decreases among amino acids. These combined physiological changes resulted in pronounced growth inhibition in pakchoi. The findings advance understanding of UV filter phytotoxicity and inform strategies for mitigating their environmental impact.
研究领域:
植物学
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环境毒理学
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植物生理学
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转录组学
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生态毒理学
✓ 已验证
产品关联
DOI
高影响因子
BIORESOURCE TECHNOLOGY
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2020
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IF 7.539
Xixi Cai,Ping Wen,Yong Yuan,Jiahuan Tang,Zhen Yu,Shungui Zhou
Nitrogen-incorporating bacteria in activated sludge play important roles in nitrogen removal in sequencing bactch reactor (SBR), but the active microorganisms and their interactions in the complex community are rarely revealed. Herein, a combining cultivation-dependent and cultivation-independent methods associated with DNA-stable-isotope probing (SIP) was applied to determine the microbes responsible for nitrogen-incorporating in SBR. Results revealed that Cytophagaceae and Sphingobacteriales were identified to be involved in nitrification, and Anaerolineae , Plasticicumulans and Elusimicrobia were responsible for denitrification. Cultivable nitrobacter and denitrifiers were isolated from the activated sludge, but they did not participate in the nitrogen-incorporating based on the SIP results. Additionally, the molecular ecological network analysis indicated that the SIP-identified nitrogen-incorporating bacteria exhibited more links with the intra-community, which might explain the failure of isolating these active bacteria. These findings add understanding of the removal of nitrogenous compounds drived by nitrogen-incorporating bacteria in actual wastewater treatment process.
研究领域:
环境微生物学
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废水处理
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微生物生态学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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