BIORESOURCE TECHNOLOGY
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2016
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IF 4.917
Lin Chen,Lanlan Zhang,Tianzhong Liu
During the study of Trentepohlia arborum it became clear that its cells are rich in lipids and carotenoids. Thus, lipid content, composition and fatty acids profiles in individual lipid classes, as well as pigment profiles, responding to different culture conditions, were further investigated. The results showed that the predominant carotenoids and lipid fraction in total lipid in this study was β -carotene and TAG, respectively. The lipid content increased significantly under high light while nitrogen-replete conditions induced the highest carotenoids content. However, only with a double stress of high light and nitrogen-deficiency it was possible to maximize the productivities of both carotenoids and lipids. Carotenoids (mainly β -carotene) accounted for ca . 5% of the microalgal lipid under the double stress. Data herein show the potential of T. arborum for the production of both lipids and carotenoids, and hence provide an appropriate way to produce different products from T. arborum .
研究领域:
藻类生物学
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生物技术
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
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2024
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IF 4.9
Wen-Juan Wang,Kai-Qi Liu,Jia-Lou Chen,Gang Wang,Feng Li,Yu-Qin Wang,Fen-Ying Kong,Wei Wang,Zhong-Xia Wang
Cancer is currently one of the deadliest diseases in the world. As one of the most common anti-cancer drugs, monitoring the amount of doxorubicin (Dox) with high sensitivity in serum can not only guide the dosage of Dox and minimize their toxicity, but also guarantee the effectiveness of treatment and the safety of patient’s life. Here, a green luminescent probe of the sulfur-enrichment, nitrogen-deficiency carbon nanodots (SCDs) was synthesized by melamine and 3-mercaptopropionic acid as nitrogen-sulfur-oxygen sources. The optical properties and surface functional groups of the SCDs have tremendously changed owing to the enrichment of the sulfur atoms. Interestingly, the SCDs luminescence can be suppressed by Dox through effective inner filter effect, which was permitted to construct high sensing platform for Dox determination. With the help of this mechanism, a wide linear range from 0.1 μM to 300 μM with a low limit of detection of 78.4 nM by utilizing SCDs-based constructed sensing platform were obtained. More importantly, this sensing platform has been successfully used to detect Dox in serum samples, and achieved satisfactory results with the recoveries between 97.7 and 104.2 %. This study conduces to the design of surface sulfur-containing carbon dots and broaden the utilization of functional SCDs in anthraquinone drugs detection.
研究领域:
生物医学
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药物分析
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纳米材料科学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Huan Yao,Jinlei Yang,Zhengmao Wang,Xingyu Pan,Junmin Pan,Hongmei Li,Sichun Zhang
Microalgae metabolite analysis is fundamental for the rational design of metabolic engineering strategies for the biosynthesis of high-value products. Mass spectrometry (MS) has been utilized for single-cell microalgae analysis. However, limitations in the detection throughput and polarities of detectable substances make it difficult to realize high-throughput screening of high-performance microalgae. Herein, a plasma-assisted label-free mass cytometry, named as PACyESI-MS, was proposed combining the advantages of orthogonal hybrid ionization and high-throughput MS analysis, which realized rapid metabolite detection of single microalgae. The cell detection throughput of PACyESI-MS was up to 52 cells/min. Dozens of the critical primary and secondary metabolites within single microalgae were detected simultaneously, including pigments, lipids, and energy metabolites. Furthermore, metabolite changes of Chlamydomonas reinhardtii and Haematococcus pluvialis under nitrogen deficiency stress were studied. Discrimination of Chlamydomonas under different nutrient conditions was realized using single-cell metabolite profiles obtained by PACyESI-MS. The relationships between the accumulation of bioactive astaxanthin and changes in functional primary metabolites of Haematococcus were investigated. It was demonstrated that PACyESI-MS can detect the flexible change of metabolites in single microalgae cells under different nutritional conditions and during the synthesis of high-value products, which is expected to become an important tool for the design of metabolic engineering-based high-performance microalgae factories.
研究领域:
生物化学
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代谢工程
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植物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSYSTEMS ENGINEERING
·
2021
·
IF 4.123
Jiyong Shi,Yueying Wang,Zhihua Li,Xiaowei Huang,Tingting Shen,Xiaobo Zou
Nitrogen (N), magnesium (Mg), and potassium (K) deficiency all result in the decrease of chlorophyll density (CH.D) in cucumber leaf, but it is difficult to differentiate N/Mg/K-deficient leaves using CH.D. New nutrient indicators based on CH.D distribution features of the whole cucumber leaf were proposed to simultaneously diagnose N/Mg/K-deficient cucumber plants. CH.D distribution map in N/Mg/K-deficient and control leaves were determined non-destructively using hyperspectral imaging with the wavelength range of 430–960 nm. Compared with the CH.D distribution map of control group leaves, a decrease in CH.D could be observed on the whole area of N-deficient leaves, Mg-deficient leaves presented pixels with low CH.D between main leaf vein, and pixels with low CH.D at the border of K-deficient leaves. Distribution features were extracted from the CH.D distribution map and were employed to diagnose N/Mg/K-deficient leaves simultaneously, with the diagnostics rates of 96.67%. The results indicated that CH.D distribution features can realize the simultaneous diagnosing in the early stage of N/Mg/K deficiency and timely guidance of top dressing is of great significance to increase yield.
研究领域:
植物营养学
·
农业遥感
✓ 已验证
DOI
高影响因子
Frontiers in Microbiology
·
2025
·
IF 4.5
Jianping Jia,Chenxi Ye,Sainan Jin,Qingqing Li,Zhengyi Pan,Wen Wen,Guoliang Bao
Astaxanthin is a strong antioxidant and is widely applied in food industry. The yeast Phaffia rhodozyma is an ideal microbial astaxanthin resource. However, the nitrogen-deficiency stress, which is beneficial for astaxanthin synthesis, often impairs cell growth, leading to low productivity. In this study, an imbalance between cellular growth and astaxanthin synthesis in P. rhodozyma under nitrogen-deficient (H) and nitrogen-sufficient (L) conditions was identified. A comparative RNA-seq transcriptome analysis between the H and L groups revealed well-discriminated patterns. The differentially expressed genes (DEGs) indicated that the regulation of nitrogen deficiency does not occur directly in the astaxanthin biosynthesis pathway but rather operates at the global cellular level, involving processes such as central and energy metabolism, antioxidative stress responses, signal transduction, competitive metabolic pathways, and material transportation. Based on these findings, a regulatory mechanism is proposed, which involves cellular sensing of nitrogen sources in the medium, alterations in signaling pathways that direct effectors, and the regulation of multiple downstream target genes through post-translational modifications, protein interactions, gene transcription, and the protein and metabolite levels. Six DEGs were overexpressed in the wild strain (WT) of Phaffia rhodozyma, and the mutants M2 and M6, expressing the NHEJ gene for DNA repair and the ferric reductase gene, showed higher biomass and astaxanthin content compared with the WT strain under nitrogen-deficient conditions. However, the remaining mutants exhibited unchanged or even reduced biomass and astaxanthin productivity. Subsequently, a co-expression mutant (M7) carrying the two DEGs was constructed. This mutant exhibited further increases in both biomass and astaxanthin content, with 61.5 and 133.3% higher yields than the WT strain, respectively, and a 265.8% increase in final astaxanthin production.
研究领域:
微生物学
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分子生物学
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代谢工程
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合成生物学
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工业生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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