FOOD CHEMISTRY
·
2022
·
IF 9.231
Jinxia Dai,Hui Lin,Yuning Pan,Yanmin Sun,Ye Wang,Jun-qin Qiao,Hong-zhen Lian,Chun-xiang Xu
Chlorpromazine (CPZ) is abused in animal husbandry and can be extensively metabolized in humans and animals. However, the actual monitoring mainly focuses on the parent compound but lacks attention to its metabolites. A method was developed and validated firstly for identification and determination of CPZ and its four major metabolites in animal-derived foods using ultrahigh-performance liquid chromatography coupled with quadrupole-Orbitrap mass spectrometry in combination with QuEChERS preparation method. Satisfactory recoveries of analytes spiked in fish and pork samples ranged from 72 to 117 %, and limits of quantification were 2.0 and 1.0 μg kg −1 for fish and pork samples respectively. Moreover, through the hydrolysis experiments of CPZ, its hydrolysates, such as CPZ-sulfoxide, CPZ- N -oxide and CPZ-sulfoxide- N -oxide, were identified as potential risk compounds. The developed method has been successfully applied to the determination of CPZ and its metabolites in actual commercial samples, as well as to the screening of other CPZ-related risk compounds.
研究领域:
食品安全
·
分析化学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BRITISH JOURNAL OF CANCER
·
2025
·
IF 6.8
Pu Yamin,Xu Fuyan,He Anqi,Li Ru,Wang Xiangxiu,Zhou Liang,Sun Hongbao,Zhang Yiwen,Xia Yong
Background Triple-negative breast cancer (TNBC) presents significant challenges due to its aggressive nature and high propensity for brain metastasis, often exhibiting resistance to standard treatments. In this study, we conducted a preliminary screening of potential therapeutic agents and identified chlorpromazine (CPZ) as a promising candidate for treating TNBC and its brain metastases. Methods The inhibitory activities of CPZ and its combination with several standard treatment drugs were evaluated in preclinical TNBC models. The mechanism of CPZ on TNBC was elucidated using TMT-labeled quantitative proteomics analysis. Results In vivo experiments demonstrated that CPZ robustly suppressed tumor growth and metastasis, particularly in lung and brain models. Importantly, CPZ enhanced the efficacy of standard therapeutic agents such as vinorelbine (NVB) and anti-PD-1 antibody. Mechanistically, CPZ induced G2/M phase arrest and triggered mitochondria-mediated intrinsic apoptosis in TNBC cells. Furthermore, CPZ triggered incomplete autophagy and activated PINK1-Parkin-mediated mitophagy. Inhibiting autophagy/mitophagy augmented CPZ’s anticancer effects, indicating these processes may have cell protective roles. Conclusions Our study highlights the dual function of CPZ in suppressing TNBC growth and metastasis, positioning it as a promising candidate for treating this aggressive cancer. Additionally, targeting autophagy/mitophagy may serve as an effective strategy to enhance anticancer therapies against TNBC.
研究领域:
肿瘤学
·
药理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Ying Pan,Congying Rao,Xiaolin Tan,Yun Ling,Ayushi Singh,Abhinav Kumar,Baohong Li,Jianqiang Liu
Rational designing of carbon-based adsorbent precursors is an important way to enhance adsorption performance. In this work, fascinating Co@C composites have been designed and fabricated using a new cobalt-seamed metal–organic nanocapsule (Co-MONC), which exhibits incredibly high adsorption capacity for chlorpromazine (CPZ) removal from aquatic environments. Benefiting from its interconnected micro- and mesoporous structures, well-dispersed and highly available Co adsorption active sites, the optimal Co@C-900 exhibits ultrahigh CPZ adsorption capacity (1039.53 mg g −1 ) and excellent recycling ability, outperforming the reported porous materials and commercial activated carbon. Moreover, the adsorption behaviors of CPZ for the composite have been comprehensively investigated and the plausible adsorption mechanism was proposed. Apart from this, the interaction mechanism between exposed Co adsorption active sites and CPZ have been suggested with the aid of density functional theory (DFT) calculations. The presented investigation demonstrated a facile and designable strategy for the fabrication of highly effectively adsorbents for organic contaminant removal.
研究领域:
环境科学
·
材料科学
·
药物化学
✓ 已验证
DOI
高影响因子
Biosensors-Basel
·
2024
·Vol. 14
·期 12
·586
·
IF 4.9
Xiaowei Fan,Guping Zhang,Xiaodi Li,Yao Wang,Yi Wang,Shilei Hao,Defang Liu
In this study, we have proposed an electrochemiluminescence (ECL) signal amplification system which is based on two-dimensional (2D) flower-like CdS@Co/Mo-MOF composites as a co-reaction accelerator of the g-C3N4/S2O82−system for ultrasensitive detection of chlorpromazine hydrochloride (CPH). Specifically, the 2D flower-like Co/Mo-MOF with mesoporous alleviated the aggregation of CdS NPs while simultaneously fostering reactant-active site contact and improving the reactant–product transport rate. This allowed the material to act as a novel co-reaction accelerator, speeding up the transformation of the S2O82−into SO4•−and enhancing the cathodic ECL emission of g-C3N4. Moreover, the signal probe which was synthesized by coupling the 2D CdS@Co/Mo-MOF and graphitic carbon nitride (g-C3N4) achieved the generation of SO4•−in situ and reduced energy loss. The results confirmed that the ECL signal was enhanced 6.2-fold and stabilized by CdS@Co/Mo-MOF. Based on the extremely strong quenching effect of chlorpromazine hydrochloride (CPH) on this system, a “signal-off” type sensor was constructed. The sensor demonstrated excellent sensitivity and linear response to CPH concentrations ranging from 1 pmol L−1to 100 μmol L−1, with a low detection limit of 0.4 pmol L−1(S/N = 3).
研究领域:
材料科学
·
分析化学
·
医学检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2019
·
IF 3.261
Limei Zhang,Ju Qin,Qiang Yang,Siqi Wei,Rui Yang
Herein, water-soluble nitrogen-doped carbon dots (N-CDs) possessing strong blue fluorescence were synthesized by one-step hydrothermal treatment using citric acid monohydrate(CA)and semicarbazide hydrochloride with NaOH as solvent. What's important in this article is that the N-CDs interacted with chlorpromazine hydrochloride (CPH), leading to the quenching of strong fluorescence. Based on the above phenomenon, a “switch-off” fluorescent probe with excellent sensitivity and superior selectivity was designed for specifically detecting CPH, and an “AND” logic gate for sensing CPH was first proposed. In this system, two linear ranges of 7.5–100.0 μM and 100.0–1000.0 μM CPH could be easily tracked, and a 0.043 μM detection limit was available. Additionally, the as-constructed sensor was conditioned for CPH determination in spiked human urine with satisfactory results, proving this is a potential and promising strategy for rapid screening of CPH in biological fluids. According to what we know, it is the first research regarding the use of CDs without any material decoration as probes to test CPH, which opens up the optical path for CPH detection.
研究领域:
分析化学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2011
·Vol. 175
·期 1
·193-199
·
IF 2.578
Yang Hui,Li Xin-chun,Yang Fan,Feng Jun,Lin Miao-yin,Chen Zuan-guang
We describe an efficient and easily fabricated electrochemiluminescence detection system for microchip capillary electrophoresis. A 300-μm-diameter platinum disc working electrode was embedded in a titanium tube which provides an adequate holding for working electrode and acts as counter electrode. We also have designed a simplified detection cell with a guide channel for the electrode. The integrated working-counter electrode can be easily aligned to the outlet of the separation channel through the guide channel. The functionality of the system was demonstrated by separation and detection of proline and tripropylamine. The response to proline is linear in the range from 5 μM to 5,000 μM, and the detection limit is 1.0 μM (S/N = 3). The system was further applied to the determination of chlorpromazine hydrochloride in pharmaceutical formulations. The system is believed to have potential applications in pharmaceutical analysis.
We described an efficient and easily fabricated electrochemiluminescence detection system for microchip capillary electrophoresis. The functionality of the system was demonstrated by separation and detection of proline and tripropylamine. The response to proline is linear in the range from 5 μM to 5,000 μM, and the detection limit is 1.0 μM (S/N = 3).
研究领域:
分析化学
·
制药工程
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL PHYSICS LETTERS
·
2021
·
IF 2.328
Xufeng Hao,Weixuan Liu,Yajun Zhang,Weijun Kang,Lingmei Niu,Lianfeng Ai
Chlorpromazine hydrochloride (CPZ) is a phenothiazine antipsychotic drug, which can effectively relieve the symptoms of chronic or severe mental disorders and reduce the manic phase of manic depression. However, excessive CPZ (more than 2 g per day) can cause serious harm to the health of the body, such as cholestatic jaundice, leukopenia, orthostatic hypotension, extrapyramidal effects and contact dermatitis. Therefore, a simple, fast, and accurate method to detect and identify CPZ is very important, especially for the detection of biological samples. In this study, based on the principle of inorganic salt inducing nanoparticle agglomeration, we synthesized silver colloids (AgNPs) with plasmon resonance effect. And we introduced inorganic salt MgBr 2 to induce self-limiting aggregation of AgNPs, resulting in a large number of “hot spots” in the nanoparticle gap. Thereby significantly enhancing the SERS intensity. The surface-enhanced Raman spectroscopy (SERS) method proposed in this paper can achieve real-time rapid detection of chlorpromazine hydrochloride in the field, with a linear range of 0.1–100 ppm (R 2 = 0.992). The calculated detection limit was 0.048 ppm. This strategy enables high stability and good repeatability of the SERS spectra. The application of established method can achieve the rapid and accurate determination of CPZ in pig urine. The recovery of standard addition was 87.50–107.70% and the RSD was between 2.87% and 7.42%. Therefore, the SERS detection technology in this study provides a new insight for the rapid, effective and accurate determination and identification of CPZ in pig urine.
研究领域:
分析化学
·
药物分析
·
生物医学
✓ 已验证
产品关联
DOI
高影响因子
ANALYST
·
2023
·Vol. 148
·期 10
·2214-2224
·
IF 4.2
Jiandan Chen,Hongying Liu,Chenwei Wang,Kai Fan,Lihua Li,Yuqing Zhang,Lu Fang,Zheng-Zhi Yin,Zhong Lü
Chlorpromazine (CPZ) is a medicine for nervous system disorders. Measuring CPZ in vivo can assist doctors in evaluating patients’ blood drug concentration and monitoring drug metabolism. Therefore, an accurate in vivo detection of CPZ is crucial. In recent years, the acupuncture needle, traditionally used in Chinese medicine, has emerged as a potential electrode in the field of electrochemistry, with promising applications for in vivo detection. In this study, Au/Cu nanoparticles were electrodeposited onto an acupuncture needle electrode (ANE) to improve electrical conductivity and provide an electro-catalytic surface. Subsequently, 3-aminophenylboronic acid and CPZ were attracted to each other through intermolecular forces; at the same time, the interaction force of Au–S between CPZ and the AuNPs made the polymer layer grow around the CPZ molecules on the modified electrode surface. The imprinted nanocavities showed highly selective and sensitive detection performance for CPZ after elution. Inside the recognizable site and microenvironment of the cavities, the captured CPZ molecule provided a suitable configuration for the fluent electron transfer of the electroactive group within a short range from the Au/Cu bimetal. Under ideal conditions, the MIP/Au/Cu/ANE exhibited two good linear ranges of 0.1–100 μM and 100–1000 μM with a detection limit of 0.07 μM. Moreover, the sensors showed great selectivity, good stability and excellent repeatability, making them suitable for CPZ detection in human serum. This provides a novel idea for real-time and in vivo CPZ detection.
研究领域:
电化学
·
分析化学
·
生物传感器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Jingxia Yuan,Shulei Wang,Lu Chen,Yiwei Liu,Faqiong Zhao,Baizhao Zeng
Single-signal based sensors generally suffer from the influence of operation condition fluctuation and environmental change, resulting in reduced accuracy. Herein a novel ratiometric electrochemical sensor based on polydopamine and ZnMn₂O₄/carbon nanotube (ZMO/MWCNTs) for the detection of antipsychotic drug chlorpromazine (CPZ) was presented. The ZMO/MWCNTs composite was employed as substrate for electrode modification, which not only facilitated electron transfer but also provided a large surface for loading molecularly imprinted polymer (MIP). Dopamine coordinated with copper ions served as the monomer, and the MIP film was prepared on the electrode surface through electropolymerization. While acting as a molecularly imprinted polymer, the polydopamine also functioned as an internal reference probe to correct the influence of condition fluctuations and enhance measurement reliability. Furthermore, the introduction of copper ions improved imprinting efficiency and reduced charge transfer resistance. Under optimized conditions, the developed Cu-MIP/ZMO/MWCNTs/GCE sensor enabled CPZ quantification over a wide linear range (1 nM-10 μM) with a detection limit of 0.42 nM. Recovery tests in human serum and lake water samples showed satisfactory results (85.0%−116.0%), with no significant difference compared to the results obtained using high-performance liquid chromatography.
研究领域:
分析化学
·
药物检测
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
ELECTROCHIMICA ACTA
·
2022
·
IF 7.336
Yongtao You,Silan Bai,Yamin Ma,Cheng Liu,Lishi Wang
ABSTRACT Phenothiazine is an intermediate in the synthesis of drugs, and its derivatives, i.e., chlorpromazine (CPZ) and promethazine (PMZ), are both the major tranquilizers. Herein, a nanoscale soft sensor constructed utilizing the interface between two immiscible electrolyte solutions (ITIES) for CPZ and PMZ drugs was introduced. A nanopipette with a 60 nm diameter orifice was used to support the ITIES , and voltammetry and amperometry were used for the qualitative and quantitative determination of these two drugs crossing the liquid/liquid (L/L) interface. Half-wave potentials ( Δ w o φ 1 / 2 ) of 0.107 V and 0.092 V (vs. Δ w o φ 1 / 2 , T E A + ) for PMZ + and CPZ + transfer from water to 1,2-dichloroethane (DCE) were obtained, respectively. Subsequently, the kinetics and effective hydrophilicity of these two drugs were thoroughly investigated. Moreover, there were two calibration curves for the determination results between the current and the concentration range for CPZ + and PMZ + by cyclic voltammetry (the concentrations range from 0.1 to 10 mmol −1 ) and amperometry (the concentrations range from 0.02 to 10 mmol −1 ). Finally, the actual drug recovery for these two drugs ranged from 94.83% to 111.02%, which indicates the accuracy in actual medicine is good. The above work provides a soft nano-liquid/liquid interfacial sensor with applications in drugs, including kinetics studies and drug determination, and many applications in detecting pharmaceutical molecules, cellular secretion and other bioorganic compounds.
研究领域:
分析化学
·
药物化学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Lingqiao Zhang,Siqi Wei,Limei Zhang,Xinghang Yin,Rui Yang
A series of nitrogen-doped carbon dots (N-CDs) are prepared under different solvent conditions from citric acid and different nitrogen sources, and a novel principle for modulating their fluorescence (FL) properties has been proposed. The nitrogen-doped carbon core can act as a fluorophore, causing diverse FL properties of N-CDs to a certain extent restricting electron delocalization. The solvent effect experiments show that the FL originates from the local emission (LE) of the carbon core. Significantly, the electron-rich substituents linked directly to the carbon core can act as electron donors, which lead N-CDs to undergo a normal “surface substituents-excited” photoinduced electron transfer (S-PET) or a nontypical “carbon core-excited” PET (C-PET) under alkaline or acidic conditions, respectively, causing an uncommon pH-sensitivity FL quenching of N-CDs. Since C-PET or S-PET dominated by pH could further regulate the surface charges of N-CDs, unseparated dual-channel specific recognition and the “AND” logic gate detection for Cu 2+ and Chlorpromazine hydrochloride (CPH) in neutral conditions have been established.
研究领域:
材料科学
·
分析化学
·
生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Shanshan Zhan,Hongying Liu,Lihua Li,Zhengzhi Yin,Chunchuan Gu
Developing fast and sensitive methods to simultaneously analyze neurotransmitter and drug is of scientific significance for diagnosing and treating neurological diseases. Acupuncture can adjust and balance the blood gas, yin and yang and other physiological states through inserting acupuncture needle into the patient’s body, so as to treat diseases, care health and strengthen body. In this study, the stainless-steel needle was used as electrode matrix for the sensor. Gold nanoparticles (Au NPs), polydopamine (PDA) and multi-wall carbon nanotubes (MWCNTs) were layer-by-layer modified onto the bare acupuncture needle (AN) through green and environment-friendly method, and the new interface showed efficient performance for simultaneous monitoring dopamine (DA), acetaminophen (AC) and chlorpromazine (CPZ). It was interesting that the synergistic interface of MWCNTs, PDA and AuNPs exhibits effect conductivity, and high electrocatalytic activity for three molecules. The sensor optimally exhibits wide linear ranges of 0.08–100 and 100–1000 μM for DA, 0.07–100 and 100–1000 μM for AC, 0.1–100 and 100–450 μM for CPZ, respectively. The low detection limits are 0.015, 0.012, and 0.022 µM correspondingly, which are competitively compared to previous reports. In addition, the sensor exhibits good anti-interference ability in the presence of excessive interference. The sensor can successfully monitor DA, AC and CPZ in human serum and urine with the recovery rates from 97.2 % to 102.6 %. The sensor showed good repeatability, stability, and reproducibility. The simultaneous detection of needlelike sensor should play important roles in biomedicine.
研究领域:
分析化学
·
生物医学工程
·
神经科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）