INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
·
IF 8.5
Liyuan Yin,Zhenjia Zheng,Qiannan Liu,Wei Liu,Ruixuan Zhao,Honghai Hu
The purpose of this study was to investigate the effect of starch gelatinization degree on the complexation mode and complexation strength of polyphenols (chlorogenic acid), as well as the changes in the physicochemical properties of potato starch with varying degrees of gelatinization before and after the addition of chlorogenic acid. The result show that the degree of gelatinization of potato starch increased from 0 % to 100 %, while the recombination rate of chlorogenic acid increased from 21.92 ± 0.67 % to 40.93 ± 0.15 %. An increase in gelatinization degree enhances the interaction between chlorogenic acid and potato starch, resulting in the formation of V-type complexes. The setback value of potato starch with 100 % pasting degree decreased from 27.74 ± 3.65 (×10 2 mPa s) to 24.78 ± 0.73 (×10 2 mPa s) after chlorogenic acid was added. The results showed that chlorogenic acid delayed the retrogradation of potato starch. The addition of chlorogenic acid enhanced the thermal stability of potato starch. The markedly bound water content (A 21 ) of potato starch-chlorogenic acid complex gels increased from 0.96 ± 0.10 % to 2.24 % ± 0.14 % as the degree of gelatinization from 0 % to 100 %, indicative of an enhanced water-holding capacity of potato starch gels by chlorogenic acid. These findings provide a theoretical foundation for expanding the use of potato starch in the food industry and improving the quality of starch-based foods.
研究领域:
食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2024
·
IF 4.1
Xuemei Ma,Yali Gao,Wanyu Pang,Xile Chang,Zhiyong Hu,Tuoping Hu
The design of efficient electrocatalysts for the determination of chlorogenic acids (CGA) is crucial in the biological and medical fields. In this paper, ZIF-67/poly(3,4-ethylenedioxythiophene) (PEDOT) nanocomposites were synthesised. The introduction of PEDOT increased the electrical conductivity of the ZIF-67/PEDOT composites, inhibited the Co atom agglomeration, led to the exposure of more active sites and increased the transport channels. An ultrasensitive CGA sensor based on ZIF-67/PEDOT nanocomposite modified carbon paper (CP) electrode was developed. The nanomaterials were characterised using scanning electron microscopy (SEM), transmission electron microscopy (TEM) and X-ray diffraction spectroscopy (XRD). Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were used to study the electrochemical properties of the modified CP electrode. In addition, the effects of different experimental conditions such as pH, scan rate and concentration on the peak current of CGA were investigated. Under the optimised conditions, the linear range of ZIF-67/PEDOT/CP for the determination of CGA was 0.1 ∼ 40 μM, and the limit of detection (LOD) was as low as 0.003 μM (S/N = 3). Furthermore, the sensor was successfully used for the quantitative analysis of CGA in real samples.
研究领域:
生物医学
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分析化学
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材料科学
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DOI
高影响因子
FOOD CHEMISTRY
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2016
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IF 4.052
Shalu Fu,Chunhua Wu,Tiantian Wu,Haixia Yu,Shuibing Yang,Yaqin Hu
Chlorogenic acid (CGA), a type of plant polyphenol, was conjugated onto gelatin (Gel) to prepare a novel coating material for the preservation of fresh seafood. The optimal reaction molar ratio of CGA to gelatin (4:1) was determined according to the CGA content in the CGA-Gel conjugate. CGA was confirmed to be successfully conjugated onto gelatin by 1 H nuclear magnetic resonance and Fourier transform-infrared spectroscopy. The antioxidant activity of CGA-Gel was proven to be higher than that of the free CGA in 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonate) radical scavenging, hydrogen peroxide scavenging, ferric ion reducing power and lipid oxidation assays. The minimum inhibitory concentrations (MIC) of CGA against Escherichia coli , Pseudomonas aeruginosa , Listeria monocytogenes and Staphylococcus aureus were 1, 1, 2 and 2 mg/mL, respectively. The antibacterial activity of CGA-Gel was unaffected by conjugation.
研究领域:
食品科学
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生物化学
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DOI
PubMed
高影响因子
Biomaterials Science
·
2024
·
IF 5.8
Huiling Zhong,Zhen Zhang,Mohong Wang,Yifei Fang,Ke Liu,Junqiang Yin,Jun Wu,Jianhang Du
Acute severe trauma is often associated with rapid blood loss and a high risk of infection. Based on these concerns, this study successfully constructed a multifunctional dual-layer bioactive sponge PCCT with rapid hemostatic and infection-preventing ability. Its external surface is an electrospun poly(lactic acid) (PLA) nanofiber thin film layer, which ensures its high air permeability and effectively protects against external bacterial invasion. In vitro results showed that the film is effectively resistant to invasion by typical Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria. The inner sponge layer was formed by chlorogenic acid (CGA) grafted with chitosan (CS) and loaded with tranexamic acid (TA). The abundant cationic groups on the sponge interacted with negatively charged erythrocytes and achieved rapid hemostasis at the wound site under the action of TA. In addition, the high porosity and bioactivity of the CS-CGA sponge scaffold endowed the hydrogel with good water absorption, antibacterial properties and anti-inflammatory activity, which effectively accelerated the healing of acute infected wounds in rats and demonstrated favorable biosafety.
研究领域:
生物材料学
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创伤修复
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药剂学
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产品关联
DOI
PubMed
高影响因子
Desalination and Water Treatment
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2024
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IF 1
Shuang Wu,Yansong Zhang,Shengnan Kong,Haiyang Yan,Chuanrun Li,Huchuan Wang
s In this paper, a hybrid system of ultrafiltration and membrane distillation was used to study the resourceful reuse of active compounds and water resources in traditional Chinese medicine wastewater. Pre-treatment of turbid wastewater by ultrafiltration (UF) was found to maximise the retention of macromolecular organic matter in the wastewater using a 10 kDa ultrafiltration membrane at a trans-membrane pressure of 0.15 MPa. The UF filtrate was subjected to vacuum membrane distillation (VMD) at 60 °C, 95 kPa, 36 L·h −1 , and pH = 3, which ultimately recovered about 85 % of chlorogenic acid as well as about 74 % of baicalin. The UF-MD system can effectively separate the large organic molecules, small molecules of active compounds and water in the turbid wastewater of traditional Chinese medicine, so as to realise the recovery and reuse of valuable resources in the turbid wastewater of traditional Chinese medicine.
研究领域:
环境科学
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中药学
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水资源管理
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产品关联
DOI
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2020
·Vol. 101
·期 4
·1579-1588
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IF 2.614
Ibrahim Khalifa,Jing Du,Asad Nawaz,Chunmei Li
BACKGROUND The effect of multiple co-pigments on the color intensification of mulberry anthocyanins (ACs) using spectroscopic techniques in combination with a molecular docking study was studied. The hyperchromicity of ACs co-pigmented with chlorogenic acid (CH) and quercetin (Q) blends was measured and their color stability in liquid and encapsulated particle models was evaluated. RESULTS Multiple co-pigments exhibited higher hyperchromicity, p K H -values, and heat-stability than their individual counterparts. Surflex-docking findings confirmed that stronger binding occurred between multiple ligands and AC than single ones due to their extra -OH, -COOH groups, and delocalization systems. The binding was allowed by increased H-bonding, van der Waals forces, and π - π sites by the extra groups of the multiple co-pigments with AC in aqueous juice and whey particle-based models. CONCLUSION This is the first report of the ternary mixture of phenolic acid-flavonol-anthocyanin which could be used as promising food red-colorants. © 2020 Society of Chemical Industry
研究领域:
食品科学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL & PHARMACEUTICAL BULLETIN
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2018
·Vol. 66
·期 6
·608-611
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IF 1.258
Jiaxin Li,Yujie Wang,Jian Xue,Pengsi Wang,Shangmei Shi
To investigate the dietary exposure risk of flonicamid application on Lonicerae Japonicae Flos and the effect of flonicamid on constituents of Lonicerae Japonicae Flos, field experiments were conducted in Fengqiu, Henan province, and flonicamid residue in samples collected was detected by gas chromatography equipped with electron capture detector (GC-ECD). And chlorogenic acid and luteoloside were determined by HPLC. Dietary exposure risk assessment was conducted through comparing the estimated daily intake (EDI) which was calculated by using the consumed residual level along with the acceptable daily intake (ADI). The effect of flonicamid on chlorogenic acid and luteoloside were obtained by ANOVA statistical analysis and least significant difference (LSD)-t test. The results showed that the terminal-residue contents of flonicamid were under 1.6 mg kg−1. And risk quotient ranged from 0.0011 to 0.0028, indicating the long-term exposure to flonicamid residual through consumption of Lonicerae Japonicae Flos in consumers was relatively low. Flonicamid could suppress the generation of luteoloside, so it was not advised to be used in L. japonica flowering phase. The study aims at providing the useful suggestion on the reasonable flonicamid usage and the reference for the establishment of maximum residue limits (MRLs) of flonicamid in Lonicerae Japonicae Flos.
研究领域:
植物学
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药理学
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食品安全
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DOI
PubMed
高影响因子
Frontiers in Molecular Biosciences
·
2025
·
IF 4
Lian Zhu,Liu Xie,Ziming Wang,Kai-Lin Li,Wei Cai
Introduction The bioactive compound 3,5-DiCQA, derived from Duhaldea nervosa, has been traditionally utilized in folk remedies for bone fractures and osteoporosis. However, its therapeutic mechanisms remain unclear. Methods We employed UHPLC-Q Exactive Orbitrap MS-based cell metabolomics to investigate the molecular mechanisms of 3,5-DiCQA in MC3T3-E1 cells. Cell proliferation was assessed via MTT assay, differentiation by alkaline phosphatase (ALP) activity, and mineralization through alizarin red staining and cetylpyridinium chloride quantification. Metabolomic profiling compared drug-treated and control groups. Results Results from MTT assays demonstrated that 3,5-DiCQA significantly promoted cell proliferation at 100 μM. Alkaline phosphatase (ALP) assays and alizarin red staining revealed enhanced osteoblast differentiation and mineralization, respectively. Calcification deposition was significantly increased in the calcified stained cells by cetylpyridinium chloride quantization, indicating that 3,5-DiCQA can promote the mineralization of MC3T3-E1 cells. Metabolomic analysis identified key metabolic changes, including the downregulation of phytosphingosine and upregulation of sphinganine and citric acid. Discussion These findings suggest that 3,5-DiCQA promotes osteoblast proliferation, differentiation and mineralization through pathways such as sphingolipid metabolism, arginine and proline metabolism, mucin type O-glycan biosynthesis and the citrate cycle (TCA cycle). This study provides insights into the therapeutic potential of 3,5-DiCQA for osteoporosis and highlights the utility of metabolomics in elucidating traditional Chinese medicine (TCM).
研究领域:
药理学
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代谢组学
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骨生物学
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DOI
PubMed
高影响因子
Food Chemistry-X
·
2025
·
IF 8.2
Sobia Naseem,Muhammad Rizwan,Wahidah H. Al-Qahtani,Ayesha Sadiqa,Awais Ahmad
Recycling food waste to extract valuable bioactive compounds (BAC) offers a groundbreaking solution to mitigate environmental challenges. The isolation and purification of BAC from food waste are getting more attention to maintain environmental sustainability. The therapeutic potential of these compounds highlights the need for innovative approaches to extracting and purifying them from by-products. In this context, the chlorogenic (CG) acid is extracted from watermelon (Citrullus lanatus) rind (WMR) using ultrasound-assisted extraction (USAE) technique and purified using the macroporous adsorption resin (MPAR) method. The extraction and purification were carried out with water, 70 % v /v ethanol/water (70 % Et), and 90 % v/v ethanol/water (90 %-Et) solvent to analyze the impact of solvent on extraction efficiency, purity, and recovery. The extracted CG acid was qualitatively analyzed via UV spectrometry and HPLC and underwent FESEM analysis while purified CG acid was characterized via FTIR and thermal analysis. The implementation of the MPAR method notably increased the yield to 4.6 mg/g of raw material using solvents comprising 90 % ethanol than water and 70 % ethanol. The recovery of CG acid significantly escalated to 88 %, enhancing the purity percentage from an initial range of 70–94 %.
研究领域:
食品科学
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环境科学
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药学
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2023
·
IF 6
Jinyang Chang,Xu Yang,Jing Li,Qingquan Fu,Jiaping Zhou,Juan Zhao,Nan Zhang,Qingdai Liu,Tianxin Wang,Hao Wang
Chlorogenic acid (CA), a natural polyphenolic antioxidant, is used in meat products to prevent spoilage due to oxidation. Transglutaminase (TGase) is an effective protein cross-linking agent applied to meat gel products to increase the elasticity of the protein gel. The present study illustrated the effect of CA at various additions (5, 20, and 100 μmol/g protein) and/or TGase (0.2 g/100g protein) on physicochemical and gel characteristics of myofibrillar protein (MP). Texture and rheological results demonstrated that the TGase improved the gel structures of oxidized MP treated with CA (5 and 20 μmol/g protein). Scanning electron microscope (SEM) result showed that TGase addition made the gel network orderly, and overcame the phenomenon induced by excessive aggregation of proteins by CA at a high dose (100 μmol/g protein). In addition, water-holding capability (WHC) and low-field NMR (LF-NMR) results demonstrated that the TGase dramatically enhanced the water-holding capability of oxidized MP gel treated with CA ( P < 0.05), and the moisture distribution became uniform. FT-IR analysis showed that reduced α-helix structure contributed to improving gel properties. SDS-PAGE indicated that TGase enhanced cross-linking of proteins. Conclusionally, TGase improved the physicochemical and gel properties of CA-modified oxidized myofibrillar proteins.
研究领域:
食品科学
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生物化学
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产品关联
DOI
高影响因子
JOURNAL OF POROUS MATERIALS
·
2025
·
IF 3.2
Zhao Dongliang,Zhou Peng,Li Xiangzhou,Song Ke
A rapid preparation method for high performance nitrogen-rich mesoporous carbon materials based on chitosan (CS) was explored, and its application potential in chlorogenic acid (CGA) enrichment. The mesoporous carbon (MgO-CSMC) was prepared using CS as carbon and nitrogen sources, as well as MgO nanoparticles as template, for rapid and high-capacity enrichment of CGA. Structural and morphological characterization revealed that nano-sized MgO as a template facilitated the formation of abundant mesopores, endowing MgO-CSMC with an exceptionally high specific surface area (1948 m²/g) and significant nitrogen (3.6%) and oxygen (7.3%) content. The adsorption capacity of MgO-CSMC for CGA reached up to 652 mg/g, and rapid adsorption was achieved. The adsorption mechanism showed that the adsorption of the MgO-CSMC for CGA was mainly physical adsorption, conforming to the Langmuir isothermal adsorption model and pseudo-second-order kinetic model. In addition, the adsorption thermodynamic analysis indicated that the adsorption process of the MgO-CSMC for CGA was exothermic, reversible and spontaneous. This work provides a novel route for the preparation of high-performance nitrogen-rich mesoporous carbon materials and an efficient and sustainable solution for the separation and purification of bioactive ingredients.
研究领域:
材料科学
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环境科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2016
·
IF 3.299
Qinqin Liu,Yan Zhao,Jiefeng Pan,Bart Van der Bruggen,Jiangnan Shen
Using molecularly imprinted technology and the phase inversion method, a chlorogenic acid molecularly imprinted chitosan membrane was prepared. Chlorogenic acid was used as the template molecule and chitosan as the film-forming material. Sulfuric acid was chosen as the cross-linking agent, in order to inhibit the swelling of chitosan membranes. The selectivity of these imprinted membranes in terms of permeability and adsorption capacity was studied. Furthermore, the chemical composition and surface structure of the membranes as well as their thermo-stability were characterized. Scatchard results show that there are two kinds of adsorption sites present on the molecularly imprinted membranes, one with high affinity sites and one with low affinity sites. For the high affinity sites, the maximum adsorption capacity was 139.57 μmol g −1 and that for the low affinity sites was 287.86 μmol g −1 . In contrast, for the non-imprinted membranes, only one kind of sites was found, with a maximum adsorption capacity of 83.84 μmol g −1 . The adsorption isotherms indicate that the adsorption isotherm curve of chlorogenic acid binding onto the imprinted membrane agrees with the Freundlich model while that of the non-imprinted membrane conforms to the Langmuir model. The permeation experiments proved that the imprinted membrane has a selective adsorption capacity for chlorogenic acid.
研究领域:
生物化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）