MICROCHIMICA ACTA
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2018
·Vol. 185
·期 7
·1-7
·
IF 5.705
Liu Qingju,Han Ping,Gong Wenwen,Wang Hui,Feng Xiaoyuan
A method is described for the determination of the pesticide chlorothalonil (CLT). It is based on the finding that citrate-capped gold nanoparticles (AuNPs) undergo aggregation on exposure to chlorothalonil. This is accompanied by a visually detectable color change from wine red to blue. The effect is due to the interaction of the cyano group of chlorothalonil with gold nanoparticles. The assay may also be performed by using a spectrometer. The ratio of absorbances at 700 nm and 520 nm (A 700 /A 520 ) linearly drops in the 5 to 100 ng·mL −1 CLT concentration range, with a 3.6 ng·mL −1 detection limit. This is below the Chinese guideline value for cucumber. The method is rather simple and does not require any modification of the AuNPs or the utilization of antibody. It was successfully applied to the determination of CLT in (spiked) cucumber samples. Recoveries ranged from 80.4 to 97.4%, and the analytical results compared well with those obtained by HPLC.
Schematic of the assay. The strong interaction of the cyano group of acetamiprid with gold nanoparticles (AuNPs) via Au-N bond induces the aggregation of gold nanoparticles, and this is accompanied by a color change from red to purple.
研究领域:
分析化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2018
·
IF 2.891
Huan He,Dan Xiong,Fengxia Han,Zhiang Xu,Bin Huang,Xuejun Pan
Chlorothalonil (CT) is widely used to control fungal diseases, and it is commonly detected in the environment. Phototransformation is an important process which removes CT from aquatic environments. The kinetics and mechanisms of CT photodegradation in solutions with and without humic acid (HA) were investigated, and the influence of dissolved oxygen (DO) on the photodegradation process was also considered. The rate constant observed in pure water was only 0.017 h −1 , but it increased to 0.083 h −1 with 5.0 mgC L −1 of HA present. Reactive species generated by HA were shown to play an important role. Moreover, the degradation of CT was promoted at higher HA concentrations. But higher DO concentrations inhibits CT degradation because of the reaction between DO and excited triplet state CT ( 3 CT*) or HA ( 3 HA*). The contributions of the reactive species tested were in the sequence 3 HA* > 1 O 2 > H 2 O 2 /O 2 − ≈ OH in a nitrogen-saturated system, but in an oxygen-saturated system the order was OH ≈ 1 O 2 > H 2 O 2 /O 2 − > 3 HA*. 3 CT* was confirmed as the active form of CT during degradation, with 4-hydroxy-chlorothalonil and 2,4,5-trichloroisophthalonitrile as the main products. Compared to direct photodegradation, CT photodegradation mediated by HA can decrease the toxicity of an aquatic environment as demonstrated through tests with Scenedesmus obliquus.
研究领域:
环境科学
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水污染控制
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DOI
高影响因子
RSC Advances
·
2019
·Vol. 9
·期 18
·9893-9898
·
IF 3.049
Qingju Liu,Ping Han,Hui Wang,Wenwen Gong,Xiaoyuan Feng
Currently known rapid determination of fungicides usually relies on antibody-based immunoassay. This paper reports a simple antibody-free colorimetric assay for chlorothalonil via the inhibition of an enzyme-triggered reaction. The enzymatic activity of glyceraldehyde-3-phosphate dehydrogenase was significantly inhibited by chlorothalonil, and the color change of NBT-PMS system induced from NADH formation was suppressed, which could be used indirectly to assay chlorothalonil. The limit of detection (LOD) was 0.05 μM with a linear range from 0.5 to 10 μM, and the detection of 1 μM chlorothalonil in solution was achieved with a naked-eye readout. In addition, the colorimetric measurement results of the cucumber samples showed a good recovery rate, although the sensitivity was less effective than the instrumental method. Nevertheless, the results demonstrates that the chlorometric method provides potential opportunities for reliable, cost-effective quantitative detection for chlorothalonil residues in vegetables.
研究领域:
食品科学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Liying Yang,Xinyu Yang,Jiatai Guo,Zhengyi Yang,Yuhang Du,Qiqi Lu,Guohua Zhong
Managing invasive plant presents a significant challenge to both the environment and the economy. There is an opportunity to address this issue by utilizing invasive plants as a sustainable and cost-effective source of valuable functional materials for environmental remediation. In this study, a promising Mikania micrantha biochar (MB) was synthesized by pyrolyzing an invasive plant, which was utilized for bacterial immobilization and adsorption of contaminants. By embedding Proteus terrae ZQ02-MB within a polyvinyl alcohol-sodium alginate gel to create a biochar-modified bacterial microsphere (BBM), the superior degradation of pesticide contaminants was observed. Taking chlorothalonil (CHT) as an example, field experiments demonstrated that BBM achieved a removal efficiency of 59.8% by the third day. Additionally, BBM not only improved the richness and diversity of soil bacteria but also enhanced the microbial community structure by the seventh day. On this basis, the adsorption capacity of MB, biofilm growth promoting ability of BBM, and the CHT mineralization ability of strain ZQ02 were validated as a joint mechanism to promote efficient CHT removal. Overall, this study proposes a composite model of invasive plant-derived biochar loaded microorganisms, which has great potential in eliminating pollutant residues and paves new paths to develop strategies for reusing invasive species resources.
研究领域:
环境科学
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微生物学
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产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2025
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IF 4.6
Qing Li,Jianghua Wang,Zikang Zhang,Yiluo Xu,Yingqiang Fu
Chlorothalonil (CHT) is a class 2B carcinogen that is capable of remaining in the environment for long periods, has an obvious bioconcentration phenomenon, and may pollute groundwater, thus it is essential to construct a simple detection method for chlorothalonil. However, common single emission fluorescent probes or dual emission fluorescent probes constructed from multi-component mixtures have low selectivity and anti-interference ability. In this work, a simple two-step hydrothermal method was used to synthesize fluorescein-modified carbon quantum dots (F-CQDs), and a dual-emission fluorescent probe was constructed to detect chlorothalonil concentration. The study found that in the presence of a certain amount of Fe 2+ , the fluorescence intensity of the fluorescence emission peaks of F-CQDs located at 435 nm and 508 nm gradually decreased with the increase of CHT concentration within 2 min. This fluorescent probe has specific recognition for CHT and shows a significant linear relationship in the range of 0.1664–3.0695 μM, with a detection limit as low as 8.6 × 10 −3 μM. The probe was successfully applied to the detection of CHT residual content in environmental water with a satisfactory average recovery, and the present study provides a new method for the preparation of dual-emission fluorescent probes and the detection of organic pesticides and shows great potential in environmental monitoring.
研究领域:
环境科学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2025
·Vol. 15
·期 1
·1-15
·
IF 3.9
Huang Minyi,Zhang Yuhao,Xu Xiang,Duan Renyan,Yang Hui
Chlorothalonil is a widely used fungicide that has a negative effect on individual movement, but its impact pathway needs further refinement. Here, the effects of exposure to chlorothalonil on the locomotion behavior of Pelophylax nigromaculatus tadpoles (GS23) were measured at three different levels (0 µg/L, 10 µg/L, and 50 µg/L), and the possible pathways of its effects were analyzed from the gut-liver axis. Chlorothalonil exposure levels of 10 µg/L and 50 µg/L significantly reduced the average speed of P. nigromaculatus tadpoles by 26% and 32.7%, respectively, and significantly decreased the locomotor frequency by 27.1% and 58.6%, respectively. Gut microbiota analysis revealed chlorothalonil exposure significantly increased the abundance of Firmicutes , while significantly decreased the abundance of Actinobacteriota , Pseudomonas , and Rhodococcus . Metabolomics analysis identified that chlorothalonil treatment changed amino acid-related metabolism pathways in the gut and liver and altered the glycerophospholipid metabolism pathway in the liver. This study indicated that chlorothalonil can affect individual locomotor abilities and interfering with the gut-liver axis of aquatic animals. These findings establish that chlorothalonil compromises aquatic organism motility through a multi-target mechanism involving gut microbiota modulation, amino acid metabolic interference, and hepatic lipid pathway disruption.
研究领域:
生态毒理学
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微生物组学
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代谢组学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2018
·
IF 6.735
Yi Chen,Xiaoxuan Su,Yiyu Wang,Shuyuan Zhao,Qiang He
Pesticide residues in riparian zones have attracted much attention in recent decades. Their accumulations potentially deteriorate microbial activity and disturb nitrogen cycle in riparian sediments. In this study, the short-term effects of chlorothalonil (CTN, a common pesticide) on microbial denitrification were explored in riparian sediments at three levels (5, 10 and 25 mg kg −1 ). No significant differences were observed between control and 5 mg kg −1 treatment; however, CTN in 10 and 25 mg kg −1 treatments deteriorated sediment health condition and microbial activity. High concentrations of CTN also significantly decreased denitrification rates ( 15 N pairing method) by 37–62%, but increased N 2 O emission in riparian sediments by 100–285%. Our data further revealed that CTN inhibited key enzyme activities responsible for microbial metabolism, and declined electron donor (NADH) and energy source (ATP) levels during denitrification. Key denitrifying enzyme activities were also suppressed by CTN, which explained the declined denitrification process and the elevated N 2 O emission. Additionally, high-throughput sequencing and quantitative-PCR analysis showed that CTN didn’t remarkably change microbial community structures and denitrifying gene abundances after short-term exposure. Overall, this study highlights that riparian pesticides could impact nitrogen cycle of the interface between terrestrial and aquatic ecosystems , potentially accelerating water pollution and global climate change.
研究领域:
环境科学
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微生物学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
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2021
·
IF 7.514
Murtaza Ali,Da-Wen Sun,Jun-Hu Cheng,Okon Johnson Esua
The effects of combined treatment (PAL-U) of plasma-activated liquid (PAL) including plasma-activated water (PAW) and plasma-activated buffer solution (PABS) and ultrasound (U) for the degradation of chlorothalonil fungicide on tomato fruit was investigated. Distilled water and buffer solution were activated by radiofrequency plasma jet for durations of 1, 3, 5, and 10 min to obtain PAL 1 to PAL 10 . Fruits were immersed in PAL for 15 min and also in distilled water with sonication for 15 min for individual treatments, and in PAL with sonication for 15 min for combined treatments. The maximum chlorothalonil fungicide residues were reduced by 89.28 and 80.23% for PAW 10 -U and PABS 10 -U, respectively. HPLC-MS characterization revealed chlorothalonil degradation pathway and formation of 2,4,5-trichloroisophthalonitrile, 2,4-dichloroisophthalonitrile, 4-chloroisophthalonitrile, isophthalonitrile and phenylacetonitrile as degradation products. Treatments also showed no negative effects on tomato quality. Therefore, PAL and PAL-U treatments could serve as effective methods for degrading pesticides on tomatoes.
研究领域:
食品科学
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环境科学
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Murtaza Ali,Jun-Hu Cheng,Da-Wen Sun
This study investigated the effect of plasma-activated liquid (PAL) including plasma-activated water (PAW) and plasma-activated buffer solution (PABS) for the reduction of chlorothalonil (CTL) and thiram (THM) pesticide residues on tomato fruit. Results revealed that the PAL obtained by using atmospheric air as the feed gas, CTL residues were decreased to 85.3% and 74.2% and THM residues decreased to 79.47 and 72.21% after treatments with PAW 10 and PABS 10 , respectively, and increasing the activation time caused a significant reduction in fungicide residues. In addition, CTL and THM residues were also decreased while increasing the activation time of PAL using Ar/O 2 as the feed gas, the concentrations of the CTL residues were decreased to 75.07 and 69.89% for PAW 10 and PABS 10 , respectively and THM residues decreased to 65.89 and 61.91% for PAW 10 and PABS 10 , respectively. Oxidation-reduction potential (ORP) and electrical conductivity (EC) were increased significantly after plasma treatment, while pH values of both solutions were decreased significantly with activation time. NO 3 − and NO 2 − concentrations of PAW increased significantly, while for PABS, NO 3 − concentration decreased but NO 2 − , with increasing the plasma activation time. Additionally, washing with PAW and PABS caused no notable negative impact on tomato fruit. Results confirmed that PAL treatments showed a significant reduction of CTL and THM fungicide residues ( p < 0.05) in tomato without affecting the quality.
研究领域:
食品科学
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2019
·
IF 8.355
Xiaoxuan Su,Yiyu Wang,Qiang He,Xuebin Hu,Yi Chen
Agricultural production causes large amounts of pesticide residues into soil, disturbing soil denitrification and N 2 O emission. Recently, biochar amendment has been regarded as the effective way to stimulate soil denitrification and reduce N 2 O emission. However, the underlying mechanism has not been well understood. Here, the effects of biochar on denitrification and N 2 O emission in the chlorothalonil (CHT)-polluted soil were explored. Biochar (50 g kg −1 ) was added to soil at the beginning of the experiment, and CHT (5 and 25 mg kg −1 ) was repeatedly applied to soil at the 30-day interval for 105 days. The obtained results showed that CHT remarkably declined soil respiration rates (52–77%) and denitrification rates (50–56%), but increased N 2 O emissions by 820–1030%. After biochar application, however, soil denitrification rates were increased by 13–26%, and N 2 O emissions were declined by 442–809% compared with non-biochar spiked soils. A further mechanism study indicated that biochar remarkably promoted the activities of microbial electron transfer chain, electron transporters (complex I, II, III and cytochrome c), and four denitrifying reductases (NAR, NIR, NOR, NOS) in CHT-polluted soils, suggesting that electron transport and consumption capacities during denitrification were stimulated by biochar. Soil denitrification was thus improved and N 2 O emission was subsequently reduced. Furthermore, biochar significantly upregulated soil denitrifying gene abundances and increased denitrifiers abundances (i.e., Enterobacter and Pseudarthrobacter ). Overall, this work will promote the current understandings of molecular mechanisms through which N 2 O emission in pesticide contaminated soils responds to biochar remediation.
研究领域:
环境科学
·
微生物学
✓ 已验证
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2019
·
IF 4.561
Shuang Gao,Yanyan Liu,Jingyu Jiang,Qiuyu Ji,Ying Fu,Lixia Zhao,Chunyan Li,Fei Ye
Chlorothalonil (CHT) suffered from its poor water solubility, which limited the application of CHT and might cause environmental pollution due to the use of organic solvents. In our research, inclusion complexes of hydroxypropyl-β-cyclodextrin (HPβCD) and β-cyclodextrin (β-CD) with CHT were formed to investigate the effect of β-CD and HPβCD on the water solubility, antifungal activity and thermal stability of CHT. The structure and characteristics of the two inclusion complexes were systematically evaluated by nuclear magnetic resonance ( 1 H NMR), scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR) and thermal gravity analysis (TGA). The results of 1 H NMR, FT-IR, SEM and XRD indicated that CHT successfully entered the cavity of β-CD and HPβCD. TGA results proved that the thermal stability of CHT was significantly improved. Molecular modelling demonstrated that cyclodextrin could effectively complex with CHT. In addition, the biological activity test showed that the complexes had better antifungal activity than original CHT. Our research can provide useful information for the more reasonable use of CHT, and cyclodextrin can be more used in agriculture to improve the characteristics of pesticides.
研究领域:
农业化学
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药学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2020
·
IF 9.038
Guiling Yang,Yanhua Wang,Jian Li,Dou Wang,Zhiwei Bao,Qiang Wang,Yuanxiang Jin
Chlorothalonil (CTL), carbendazim (CBZ), and prochloraz (PCZ) are fungicides widely used in many countries. The use of these fungicides raises concerns because they are often applied together or used in the same agricultural area. However, the toxicity of these fungicides or mixtures, especially to aquatic organisms, has received limited attention. Here, embryonic and larval zebrafish were exposed to indicated concentrations of CTL, CBZ, and PCZ and their binary (CTL+CBZ, CTL+PCZ and CBZ+PCZ) and ternary (CTL+CBZ+PCZ) mixtures for 24 h. Based on metabolomics analysis, we observed that hundreds of metabolites were altered, and glycolysis metabolism and amino acid metabolism were the two most affected pathways. Interestingly, a total of 9 and 26 metabolites changed significantly in embryos and larvae treated with all fungicides, respectively. Among these altered metabolites, 2-aminoadipic acid (2-AAA) levels increased significantly in all groups, indicating that 2-AAA potentially represents a useful biomarker for evaluating the toxicity of fungicides. Furthermore, the joint effects of CTL+PCZ on embryos and larvae, especially on amino acid metabolism, were weaker than those in other groups, but combined treatment did not influence individual fungicidal activity. Data acquired from metabolomics provided important insight for understanding the mechanism by which fungicides or their mixtures affect zebrafish.
研究领域:
环境科学
·
毒理学
·
水生生物学
·
代谢组学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）