CHEMOSPHERE
·
2024
·
IF 8.1
Tianzhu Guan,Ning Li,Ya Gao,Mingyuan Gao,Qin Hu,Yajun Gao,Lixia Xiao,Zhenquan Yang,Qiaoquan Liu
Latest observations indicated that exposure of organic environmental neurotoxins may increase the potential risk of Alzheimer's diseases (AD). As a suspected food-derived risk factor, permethrin, composed of cis -isomer and trans -isomer, is widely used as a broad-spectrum pyrethroid insecticide in agricultural crops for the arthropod pests controlling. Thus, evaluating the impact of permethrin exposure is of great importance to human health. In this study, we performed the toxicological network approach to decipher AD-related mechanisms of cis -permethrin and trans -permethrin. Based on the toxicological network construction and central network topological analysis, human serum albumin (HSA) was selected as the core targets in AD-related developing. From the analysis of the steady state and time-resolved fluorescence quenching of HSA in presence of permethrin mixture, it has been inferred that the nature of the quenching mainly originates from the dynamic modes. Experimentally, the thermodynamic parameters revealed hydrophobic interactions and van der Waals forces played a major role during quenching process. Tryptophan synchronous fluorescence spectra were blue shifted whereas the position of tyrosine synchronous spectra was red shifted during the complex formation. Three-dimensional fluorescence together with FT-IR experiment confirmed that permethrin caused the secondary structure changes in HSA. To better understand the binding patterns between HSA and cis/trans -permethrin, theoretical calculation and molecular docking were implemented. According to the electrostatic potential map, the electrophilic attack region corresponds for electron rich oxygen atoms, while the nucleophilic attack regions were mainly located at over the benzene rings and methyl on cyclopropane ring of permethrins. Docking results shown that cis -permethrin and trans -permethrin located in hydrophobic pocket nearby Domain IIA with the different binding affinity (−7.6 and −9.2 kcal/mol), which consistent with the competitive displacement experiment. All these findings generated in the present study facilitated the elucidation of the molecular mechanism details between permethrin mixture and HSA, which provided fresh insights into the links between environmental exposure and AD-related adverse health outcomes.
研究领域:
环境毒理学
·
神经生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Binghai Lv,Zhenyan Zhang,Bingfeng Chen,Siqi Yu,Minglong Song,Yitian Yu,Tao Lu,Liwei Sun,Haifeng Qian
The application of pesticides increases crop yields but affects the structure and function of the soil microbial community. Halogens are common functional modification groups in chemical compounds, and innovative pesticides have been developed on the basis of these groups. However, the effects of different halogen substituents on soil microorganisms remain unclear. This study investigated the effects of three pyrethroid pesticides (deltamethrin, cypermethrin, and cyfluthrin) on the soil microbiota. Our results revealed that all these pesticides significantly reduced the stability of the bacterial communities and decreased bacterial diversity at high concentrations. Compared with deltamethrin (Br − ) and cypermethrin (Cl − ), low concentrations (0.5 mg/kg) of cyfluthrin (F − ) increased soil bacterial diversity by 23.14 % and increased the potential for nitrogen fixation by 2.00 % and nitrification by 3.39 %, thus making it a relatively eco-friendly option. Our findings provide new insights into the potential ecological effects of halogenated pyrethroid pesticides on soil ecosystems .
研究领域:
环境科学
·
微生物学
·
土壤学
·
生态毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2017
·
IF 3.154
Xueke Liu,Peng Wang,Chang Liu,Yiran Liang,Zhiqiang Zhou,Donghui Liu
Beta-cypermethrin (beta-CP), an important pyrethroid insecticide, and its main acid metabolites are frequently detected in human samples. Because beta-CP may pose some risk to human health, we studied dynamics and residues of beta-CP and its metabolites in hen egg, droppings, blood, and 15 other tissues after continuous exposure. A digestive model was then used to study beta-CP’s digestive fate. Beta-CP and its metabolites significantly accumulated in tissues with high lipid contents and were readily transferred to eggs. Beta-CP was mainly metabolized into acid metabolites that accumulated in egg and edible tissues of laying hens, suggesting that humans may be exposed to beta-CP acid metabolites through food.
研究领域:
毒理学
·
食品科学
·
动物科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Yuxia Yang,Yue Tang,Chunxiao Wang,Bangyan Liu,Yuangen Wu
Pyrethroid pesticides residues will not only pollute the environment, but also cause high toxicity to the human body. It is significant to establish an efficient and accurate method for pyrethroid detection in food. Considering that the common biomolecules like antibody is complicated and easy to inactivate, it is urgent to find a new type of biomolecule to specifically recognize pyrethroid pesticides. This study proposed the Capture-SELEX strategy to firstly select λ-cyhalothrin aptamer by immobilizing random ssDNA library. High-throughput sequencing was performed on the enriched ssDNA library through multiple Capture-SELEX rounds. Comprehensively inspecting structural similarity and homology , six sequences were chosen from five families for further analysis. The results showed that the aptamer (named LCT-1) could specifically recognize λ-cyhalothrin with the strongest affinity ( K d = 50.64 ± 4.33 nmol L −1 ). Molecular docking results revealed that the binding sites between λ-cyhalothrin and LCT-1 aptamer are mainly related to the bases A-5, C-6, C-28, A-29, C-30, G-31 and G-32. The LCT-1 aptamer was truncated to a shorter sequence (named as LCT-1-39) by removing other irrelevant bases, and its K d value was determined as (10.27 ± 1.33) nmol·L −1 by Microscale Thermophoresis (MST). Both LCT-1 and LCT-1-39 aptamers were employed as recognition molecules to establish the colorimetric aptasensors for λ-cyhalothrin detection, which displayed good repeatability and reproducibility. The detection limit of the aptasensors were individually calculated as 0.0197 μg ml −1 and 0.0186 μg ml −1 , and their recovery rate of λ-cyhalothrin in pear and cucumber samples was in the range of 82.93–95.50%. This article provides a promising application for the detection of λ-cyhalothrin.
研究领域:
食品安全检测
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
AQUATIC TOXICOLOGY
·
2018
·
IF 3.884
Meng Li,Qiong Wu,Qiangwei Wang,Dandan Xiang,Guonian Zhu
In aquatic environment, the presence of nanoparticles (NPs) has been reported to modify the bioavailability and toxicity of the organic toxicants. Nevertheless, the combined toxicity of NPs and the pesticides that were used world-widely still remains unclear. Cypermethrin (CYP), a synthetic pyrethroid insecticide, is commonly used for controlling agricultural and indoor pests. Therefore, the effects of titanium dioxide NPs (nTiO 2 ) on CYP bioconcentration and its effects on the neuronal development in zebrafish were investigated in our study. Zebrafish embryos (2- hour-post-fertilization, hpf) were exposed to CYP (0, 0.4, 2 and 10 μg/L) alone or co-exposed with nTiO 2 (1 mg/L) until 120-hpf. nTiO 2 is taken up by zebrafish larvae and also it can adsorb CYP. The zebrafish body burdens of CYP was observed and CYP uptake was increased by nTiO 2 , indicating that the nTiO 2 could accelerate the bioaccumulation of CYP in larvae. Co-exposure of nTiO 2 and CYP induced the generation of reactive oxygen species. Exposure to CYP alone significantly decreased the mRNA expression of genes, including glial fibrillary acidic protein (gfap) , α1-tubulin , myelin basic protein (mbp) and growth associated protein (gap-43) . Besides, reductions of serotonin, dopamine and GABA concentrations were observed in zebrafish and the larval locomotion was significantly decreased in response to the lower level of the neurotransmitters. Moreover, co-exposure of nTiO 2 and CYP caused further significantly decreased in the locomotion activity, and enhanced the down-regulation of the mRNA expression of specific genes and the neurotransmitters levels. The results demonstrated that nTiO 2 increased CYP accumulation and enhanced CYP-induced developmental neurotoxicity in zebrafish.
研究领域:
环境科学
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毒理学
·
水产生物学
✓ 已验证
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2015
·
IF 4.321
Jianming Pan,Jun Zeng,Qin Cao,Heping Gao,Yongchao Gen,Yinxian Peng,Xiaohui Dai,Yongsheng Yan
In this work, the hierarchical porous foams (HPFs) were fabricated and applied as the novel adsorbent for simultaneous removal of λ-cyhalothrin (LC) and Cu(II). Macroporous poly(glycidyl methacrylate) foams (MPGMA) with highly interconnected pore network were firstly prepared by high internal phase emulsions (HIPEs) template. Then carboxylated mesoporous silica nanoparticles (CMSNs) were grafted onto the interface of macroporous foams via the reaction between epoxy groups on MPGMA and carboxyl groups on CMSNs . The as-prepared HPFs composites possessed macropores ranging from 10 μm to 40 μm and uniform mesopores on average of 2.5 nm, and their large amount of carboxyl groups resulted in a favorable adsorption for λ-cyhalothrin (LC) and Cu(II). In single adsorption, the maximum adsorption capacity of HPFs for LC and Cu(II) were 80.11 mg g −1 and 21.79 mg g −1 at 288 K, respectively. The adsorption mechanism between LC and HPFs could be the hydrogen bonding and hydrophobic interaction, while the electrostatic attraction maybe the main interaction between Cu(II) and HPFs. Binary adsorption showed that the enhanced adsorption capacity of LC was 104.1 mg g −1 in the presence of 15 mg L −1 Cu(II) at 288 K, and the excellent result of Cu(II) was 22.23 mg g −1 with the mixed solution of 50 mg L −1 LC at 288 K, indicating the existence of promotion in adsorption ability in the case of coexistence of LC and Cu(II). Moreover, HPFs could maintain stability after five adsorption/desorption cycles in the regeneration steps.
研究领域:
环境科学
·
材料科学
✓ 已验证
DOI
高影响因子
Analytical Methods
·
2013
·Vol. 6
·期 3
·915-923
·
IF 1.855
Zhilong Song,Chunbo Liu,Jianming Pan,Yongsheng Yan,Xiao Wei,Minjia Meng,Ping Yu,Lin Gao,Jiangdong Dai,Shuang Lin
Porous silica nanospheres were fabricated using a facile surface-protected etching strategy. Polyvinylpyrrolidone (PVP) was used as a protecting polymer adsorbed on the surface of silica nanospheres and NaOH was employed as an etching agent. Owing to the protective action of PVP and inhomogeneous etching, mesopores were created in the silica nanospheres. Then, based on a simple and economical wet-chemical route, highly luminescent YVO4:Eu3+ nanocrystals were then integrated onto the nanospheres to form a highly luminescent mSiO2/YVO4:Eu3+ composite material. This material, which combined the mesoporous structure of SiO2 and the strong red luminescence property of YVO4:Eu3+, could be used as a novel optosensing system. The structure, morphology, porosity, and optical properties of the materials were well characterized by X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy, N2 adsorption/desorption, and photoluminescence spectra, and the optical stability and effect of pH on mSiO2/YVO4:Eu3+ were also evaluated. It was observed that λ-cyhalothrin (LC) could quench the luminescence of mSiO2/YVO4:Eu3+ in a concentration-dependent manner, which was best described by a Stern–Volmer-type equation. This study therefore demonstrated that the composite material could be potentially used as a probe to monitor the residues of LC in water.
研究领域:
材料科学
·
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Engineering Materials
·
2023
·
IF 0
Minghui Wan,Saijie Song,Wenli Feng,Xiaoyuan Wang,Jian Shen
A pesticide delivery system (PDS) is an effective strategy to overcome the low efficiency of conventional pesticide formulations. UiO-66, a metal–organic framework (MOF), is a suitable nanocarrier for pesticide delivery due to its large surface area, good stability, and nontoxicity. In this work, we developed deltamethrin-loaded UiO-66 (DM@UiO-66) as a PDS for sustained DM release and enhanced pest control. DM was loaded on UiO-66 via a physical adsorption method, forming a stable DM@UiO-66 nanoformulation (loading capacity 1049.6 mg/g). Unlike the burst release of free DM, DM@UiO-66 exhibits sustained pesticide release performance, which can maintain effective insecticidal concentration for a long time. Biological assay results indicate that DM@UiO-66 possesses better insecticidal activity than free DM, and the nanoformulation is safe for maize seeds and seedlings. In addition, DM@UiO-66 contains no toxic organic solvents or additives, which makes it environmentally friendly. Thus, the UiO-66-based PDS will hold a great future in agriculture and pest control fields.
研究领域:
材料科学
·
农药学
·
纳米技术
·
环境科学
✓ 已验证
DOI
BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Jixiang Wang,Hao Qiu,Hongqiang Shen,Jianming Pan,Xiaohui Dai,Yongsheng Yan,Guoqing Pan,Börje Sellergren
Molecularly imprinted fluorescent polymers have shown great promise in biological or chemical separations and detections, due to their high stability, selectivity and sensitivity. In this work, molecularly imprinted fluorescent hollow nanoparticles , which could rapidly and efficiently detect λ-cyhalothrin (a toxic insecticide) in water samples, was reported. The molecularly imprinted fluorescent sensor showed excellent sensitivity (the limit of detection low to 10.26 nM), rapid detection rate (quantitative detection of λ-cyhalothrin within 8 min), regeneration ability (maintaining good fluorescence properties after 8 cycling operation) and appreciable selectivity over several structural analogs. Moreover, the fluorescent sensor was further used to detect λ-cyhalothrin in real samples form the Beijing-Hangzhou Grand Canal Water. Despite the relatively complex components of the environmental water, the molecularly imprinted fluorescent hollow nanosensor still showed good recovery, clearly demonstrating the potential value of this smart sensor nanomaterial in environmental monitoring.
研究领域:
环境科学
·
分析化学
✓ 已验证
DOI
高影响因子
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
·
2019
·Vol. 246
·期 1
·239-251
·
IF 2.056
Ma Pei,Wang Luyao,Xu Lei,Li Junying,Zhang Xuedian,Chen Hui
Chlorpyrifos is a type of organophosphate insecticide used extensively in the plantation of tomatoes, a worldwide popular food. However, chlorpyrifos residues on tomatoes bring risks to human health considering the consumption level of tomato is relatively high across many countries. Accurate detection and strict regulation of chlorpyrifos residues are very important in controlling health risks related to chlorpyrifos. Therefore, we developed a rapid, highly sensitive, economical, and nondestructive surface-enhanced Raman spectroscopy (SERS)-based method that can quantitatively determine the content of chlorpyrifos in tomatoes. Silver colloid was optimized and used as the enhance substrate for SERS. Chlorpyrifos residues on tomato surface and in tomato extract were both measured with SERS, and the results acquired under these practical conditions well matched those of chlorpyrifos standard solutions. Linear relationships between chlorpyrifos concentrations and SERS intensities at crucial characteristic peaks under both conditions were established, and the results showed linearity across the entire measured range from 10 −3 to 10 −9 mol L −1 . The detection limit of the proposed method was 10 −9 mol L −1 , which was much lower than the standards of chlorpyrifos use for China, Japan and EU, and was the lowest reported concentration chlorpyrifos had been measured on any fruit/vegetable surface or in its extract. The proposed method was very promising in the accurate, quantitative detection of pesticide residues, and it may be adapted to a variety of agricultural products and other areas in food science.
研究领域:
食品安全
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2013
·
IF 3.334
Lin Gao,Xiuying Li,Qi Zhang,Jiangdong Dai,Xiao Wei,Zhilong Song,Yongsheng Yan,Chunxiang Li
In this study, we first present a general protocol for making fluorescent molecularly imprinted polymer microspheres via precipitation polymerisation. We first prepared the fluorescent molecularly imprinted polymer microspheres upon copolymerisation of acrylamide with a small quantity of allyl fluorescein in the presence of cyhalothrin to form recognition sites without doping. The as-synthesised microspheres exhibited spherical shape, high fluorescence intensity and highly selective recognition. Under optical conditions, polymer microspheres were successfully applied to selectively and sensitively detect cyhalothrin, and a linear relationship could be obtained covering the lower concentration range of 0–1.0 nM with a correlation coefficient of 0.9936 described by the Stern–Volmer equation. A lower limit of detection was found to be 0.004 nM. The results of practical detection suggested that the developed method was satisfactory for determination of cyhalothrin in honey samples. This study therefore demonstrated the potential of molecularly imprinted polymers for detection of cyhalothrin in food .
研究领域:
分析化学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2014
·
IF 4.058
Mengying Gan,Jianming Pan,Yunlei Zhang,Xiaohui Dai,Yijie Yin,Qin Qu,Yongsheng Yan
Lignin particles were used as stabilizers to establish a stable Pickering oil–water emulsion, and then the molecularly imprinted polymers (MIPs) were fabricated by radical polymerization in the presence of functional and cross-linked monomers in the oil phase. As-prepared MIPs were observed with a diameter from 10 μm to 65 μm when oil/water ratio increased from 0.11 to 0.68, and exhibited thermal stability and hydrophobic surface. A series of static adsorption experiments were conducted to analyze their adsorption performance for lambda-cyhalothrin (LC). The preliminary results showed that MIPs possessed good recognition toward LC , that is, the adsorption equilibrium time was about 3.0 h and the equilibrium adsorption amount was 72.25 μmol g − 1 for MIPs and 43.10 μmol g − 1 for nonimprinted Polymers (NIPs), respectively, indicating the enhanced selectively adsorption of LC. The adsorption process followed pseudo-second-order model by the kinetic analysis and Freundlich equation by the isothermal analysis, respectively. The selective recognition experiments displayed the outstanding selective adsorption effect of the MIPs for target LC. Moreover, the obtained MIPs could be effectively regenerated and recycled at less three times without significant loss of adsorption capacity.
研究领域:
材料科学
·
环境科学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）