ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Nan Hu,Meng Sun,Nan Lv,Yangyang Gao,Xiaohong Fu,Dayi Xing,Xiang Guo,Shaodong Zhai,Ruiping Zhang
Myocardial infarction (MI) is the leading cause of death worldwide. The most effective way to treat myocardial infarction is to rescue ischemic cardiomyocytes. After an ischemic event, the overproduction of reactive oxygen species (ROS) is a key driver of myocardial injury. The produced ROS affects mitochondrial function and induces apoptosis in cardiomyocytes. This was accomplished by constructing platelet-membrane-encapsulated ROS-responsive drug-releasing nanoparticles (PMN@NIC-MalNPs) to deliver malonate and niclosamide (NIC). The results revealed that PMN@NIC-MalNPs degraded and released malonate and niclosamide in a high-level ROS microenvironment, effectively reducing the oxidative stress and apoptosis rate. By enhancing basal mitochondrial oxygen consumption rate (OCR), adenosine triphosphate (ATP) production, and spare respiratory capacity (SRC) in vitro, reduced the oxidative stress levels and restored mitochondrial function. In vivo studies revealed that the PMN@NIC-MalNPs improved cardiac dysfunction, inhibited succinate dehydrogenase (SDH) activity, increased ATP production, and reduced the myocardial infarct size in myocardial infarction model mice. Further, transcriptome analysis and Western blot revealed that PMN@NIC-MalNPs prevented apoptosis by activating the expressions of the signal transducer and activator of transcription 3 (STAT3) and Bcl-2, and inhibiting the expression of Bax. Thus, this study provides a novel therapeutic solution for treating myocardial infarction and predicting the viability of an antioxidant and antiapoptotic therapeutic solution in the treatment of myocardial injury.
研究领域:
心脏病学
·
药学
·
纳米技术
·
分子生物学
·
治疗学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
IONICS
·
2023
·Vol. 29
·期 8
·3373-3384
·
IF 2.8
Wan Xiuying,Zhao Mengyuan,Li Fang,Zhu Gan,Zhao Hongyuan
Herein, we reported a novel niclosamide (NA) electrochemical sensor based on the multifunctional nanocomposite of palygorskite (Pal) nanofibers and bifunctional multiwalled carbon nanotubes (BMCN) with graphitization and carboxylation. For the BMCN@Pal nanocomposite, BMCN with one-dimensional nanotube structure possessed excellent electrical conductivity and good dispersion performance due to the graphitization and carboxylation; Pal with fibrous structure possessed good electrocatalytic activity due to the high absorption capability, large specific surface area, and prominent stability. Moreover, BMCN with interconnected carbon conductive network promoted the uniform distribution of Pal nanofibers and compensated for the poor electrical conductivity of Pal nanofibers. Thanks to the functional combination of BMCN and Pal, the BMCN@Pal/GCE sensor exhibited good NA electrochemical detection performance with low limit of detection of 7.88 nM in linear NA concentration of 0.01–10 μM. The BMCN@Pal/GCE sensor exhibited good repeatability and reproducibility. In addition, a good practical feasibility can be achieved at the BMCN@Pal/GCE sensor for the detection of NA in tap water and pond water samples (recovery rate: 96.2–100.3%, relative standard deviation: 2.53–4.97).
研究领域:
材料科学
·
电化学
·
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Life-Basel
·
2024
·Vol. 14
·期 5
·544
·
IF 3.2
Hao Wu,Xiping Yuan,Xing Tian,Jinwei Gao,Min Xie,Zhonggui Xie,Rui Song,Dongsheng Ou
Niclosamide (NIC) is a potent salicylanilide molluscicide/helminthicide commonly utilized for parasite and mollusc control in aquatic environments. Due to its persistent presence in water bodies, there is growing concern regarding its impact on aquatic organisms, yet this remains inadequately elucidated. Consequently, this study aims to assess the hepatotoxic effects and detoxification capacity of black carp (Mylopharyngodon piceus) in a semi-static system, employing various parameters for analysis. NIC was applied to juvenile black carp at three different concentrations (0, 10 and 50 μg/L) for 28 days in an environmentally realistic manner. Exposure to 50 μg/L NIC resulted in an increase in hepatic lysozyme (LYZ), alkaline phosphatase (ALP), and complement 4 (C4) levels while simultaneously causing a decrease in peroxidase (POD) activity. Additionally, NIC exposure exhibited a dose-dependent effect on elevating serum levels of LYZ, ALP, complement 3 (C3), C4, and immunoglobulin T (IgT). Notably, the mRNA levels of immune-related genestnfα,il8, andil6, as well asnrampandleap2, were upregulated in fish exposed to NIC. RNA-Seq analysis identified 219 differentially expressed genes (DEGs) inM. piceusafter NIC exposure, with 94 upregulated and 125 downregulated genes. KEGG and GO analyses showed enrichment in drug metabolism pathways and activities related to oxidoreductase, lip oprotein particles, and cholesterol transport at 50 μg/L NIC. Additionally, numerous genes associated with lipid metabolism, oxidative stress, and innate immunity were upregulated in NIC-exposedM. piceus. Taken together, these findings indicate that NIC has the potential to cause hepatotoxicity and immunotoxicity inM. piceus. This research offers important insights for further understanding the impact of molluscicide/helminthicide aquatic toxicity in ecosystems.
研究领域:
环境科学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2024
·
IF 6.2
Hao Wu,Xiping Yuan,Yong He,Jinwei Gao,Min Xie,Zhonggui Xie,Rui Song,Dongsheng Ou
Niclosamide (NIC) is a commonly used insecticide and molluscicide in the prevention and treatment of parasitic diseases in fish. The utilization of NIC has the potential to disrupt the microbial community present on the mucosal tissue of fish, leading to localized inflammatory responses. The objective of this study was to evaluate the impact of NIC on the immune system and bacterial populations within the gill and gut of Mylopharyngodon piceus . Fish were subjected to varying concentrations of NIC, including a control group (0 μg/L), a low NIC group (15% 96 h LC50, LNG, 9.8 μg/L), and a high NIC group (80% 96 h LC50, HNG, 52.5 μg/L). Gill and gut samples were collected 28 days post-exposure for analysis. The findings revealed that the 96-h LC 50 for NIC was determined to be 65.7 μg/L, and histopathological examination demonstrated that exposure to NIC resulted in gill filament subepithelial edema, exfoliation, degeneration, and a decrease in gill filament length. Furthermore, the gut exhibited apical enterocyte degeneration and leucocyte infiltration following NIC exposure. Additionally, NIC exposure led to a significant elevation in the levels of immunoglobulin M (IgM), complement component 3 (C3), and complement component 4 (C4) in both gill and gut tissues. Moreover, the activity of lysozyme (LYZ) was enhanced in the gill, while the activities of peroxidase (POD) and immunoglobulin T (IgT) were increased in gut tissue. The exposure to NIC resulted in enhanced mRNA expression of c3 , c9 , tnfα , il6 , il8 , and il11 in the gill tissue, while decreasing c3 and il8 expression in the gut tissue. Furthermore, the natural resistance-associated macrophage protein ( nramp ) mRNA increased, and liver-expressed antimicrobial peptide 2 ( leap2 ) mRNA decreased in gill and gut tissues. And hepcidin ( hepc ) mRNA levels rose in gill but fell in gut tissue. NIC exposure also led to a decrease in gill bacterial richness and diversity, which significantly differed from the control group, although this separation was not significant in the gut tissue. In conclusion, the administration of NIC resulted in alterations in both the immune response and mucosal microbiota of fish. Furthermore, it was noted that gills displayed a heightened vulnerability to sublethal effects of NIC in comparison to gut tissues.
研究领域:
水生毒理学
·
免疫学
·
微生物学
·
鱼类生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Electrochemical Science
·
2025
·
IF 2.4
Yuanhang Cao,Chonggao Liang,Jiale Han,Aodi Luo,Hongyuan Zhao,Runqiang Liu
An ultrasound-assisted synthesis method was employed to prepare β-cyclodextrin (β-CD) and acetylene black (AB) nanocomposites, referred to as β-CD@AB. These nanocomposites were subsequently modified onto glassy carbon electrodes (GCE) to develop electrochemical sensors for the detection of niclosamide (NA). The bead chain-like carbon network structure of AB exhibits outstanding electrical conductivity, significantly improving electrocatalytic performance. Additionally, β-CD not only enhances the dispersibility of AB but also provides superior adsorption and molecular recognition capabilities, thereby increasing the capacity of AB to capture and enrich NA molecules. The integration of β-CD and AB enabled highly sensitive NA detection, achieving a detection limit of 0.019 μM within the concentration range of 0.09~15 μM. The β-CD@AB/GCE-based analytical platform exhibited remarkable precision and accuracy in NA quantification in complex matrices, with relative normal deviations ranging from 1.26% to 4.31% and quantitative recovery rates between 96.87% and 101.02%. This electrochemical detection system demonstrated excellent operational stability when applied to actual environmental samples. The findings indicate that the sensor, developed through the modification of GCE with β-CD@AB, provides reliable performance for NA detection.
研究领域:
分析化学
·
环境科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2026
·
IF 9.8
Gan Zhu,Meimei Guo,Mengyuan Zhao,Guifang Wang,Hongyuan Zhao
In this study, the glassy carbon electrode (GCE) was embellished with carbon nanoflowers (CNF)@halloysite nanotubes (Hal)@graphene nanoplates (Gr) nanocomposite to fabricate the CNF@Hal@Gr/GCE sensor for the sensitive determination of niclosamide (NA). CNF with unique petal-shaped structure had good conductivity and abundant active sites, which could accelerate the rate of electron transfer and provide large specific surface area. Hal with outstanding physical adsorption capacity could enrich NA molecules on the working electrode surface. Gr with nano-plates structure exhibited superior electrical conductivity and larger specific surface area. The CNF@Hal@Gr nanocomposite not only increased the contact area of working electrode with NA molecules but also possessed excellent electrical conductivity and adsorption properties. The CNF@Hal@Gr/GCE sensor exhibited ultrasensitive NA detection performance, including wide linear range (0.01–20 μM) and low limit of detection (5.83 nM). Moreover, the CNF@Hal@Gr/GCE sensor presented superior practical applicability with relative standard deviations of 0.87–4.71% and recoveries of 95.18–103.1%.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Fang Li,Runqiang Liu,Volodymyr Dubovyk,Qiwen Ran,Bo Li,Yuqi Chang,Hongliang Wang,Hongyuan Zhao,Sridhar Komarneni
Herein, a simple, low-cost, environment-friendly strategy was proposed to prepare the composite of three-dimensional hierarchical porous carbon and chitosan, which was applied to modify the glass carbon electrode to fabricate an electrochemical sensor for the determination of niclosamide. The three-dimensional porous carbon with interconnected conductive network, high surface area, and self-generated oxygen-containing functional groups was prepared by salt-templating method with glucose as carbon source and eutectic mixture of LiBr/KBr as both activating and pore-forming agent. During the subsequent ultrasonic process, chitosan with excellent filming property, strong adsorption ability, and good dispersibility was successfully decorated on the obtained porous carbon to further enhance the determination performance of niclosamide. Benefitting from the multi-functional integration of three-dimensional hierarchical porous carbon and chitosan, the fabricated sensor presented a low limit of detection (6.7 nM) in the linear concentration range from 0.01 to 10 µM. Moreover, the fabricated sensor could show good repeatability, reproducibility, stability, and selectivity. Most important, the decent practicability for the detection of niclosamide was obtained in different food samples with low relative standard deviation and satisfactory recoveries. This work provides a very valuable reference for the sensitive determination of niclosamide in food samples.
研究领域:
分析化学
·
环境科学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Hongyuan Zhao,Yuqi Chang,Runqiang Liu,Bo Li,Fang Li,Fulong Zhang,Mingwang Shi,Lin Zhou,Xiaoguang Li
We report a scalable and controllable ultrasound-assisted strategy for the preparation of Vulcan XC-72 nanoparticles-decorated halloysite nanotubes (HNTs@VXC-72), which was applied to modify glassy carbon electrode (GCE) for the highly sensitive electrochemical determination of niclosamide (NA). For the HNTs@VXC-72 nanocomposite, VXC-72 nanoparticles with excellent electrical conductivity and good dispersing property contributed to the formation of the interconnected conductive network; HNTs possessed good adsorption performance and promoted the electrochemical redox reaction. The research results showed that the combination of VXC-72 nanoparticles and HNTs produced the effect of synergistic enhancement. The HNTs@VXC-72/GCE sensor could show a relatively low detection limit of 3.28 nM in the great linear NA concentration range of 0.01–1 µM. When used for the NA determination in food samples, the HNTs@VXC-72/GCE sensor exhibited good practical feasibility with low RSD and acceptable recoveries, which provided a promising NA determination approach to ensure food safety.
研究领域:
材料科学
·
分析化学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Hae-Ni Jung,Da-Hee Park,Kyung-Hee Yoo,Hee-Jung Cho,Jae-Han Shim,Ho-Chul Shin,A.M. Abd El-Aty
Herein, an analytical method was developed for simultaneous determination of 12 anthelmintics (closantel, niclosamide, nitroxynil, rafoxanide, cymiazole, fluazuron, levamisole, morantel, praziquantel, pyrantel, thiophanate, and trichlorfon) in fishery products (eel, flatfish, and shrimp) using liquid-liquid extraction coupled with liquid chromatography-tandem mass spectrometry. A reversed-phase analytical column was then used to separate the analytes from various matrices. Linear matrix-matched calibration curves were generated with coefficients of determination ≥ 0.9935. Recovery rates at three spiking levels (5, 10, and 20 µg/kg) ranged between 61.58% and 119.37% with relative standard deviations ≤ 19.05%. Limits of detection were in the range of 0.3–1.6 μg/kg, whereas limits of quantification ranged between 1.0 and 5.0 μg/kg. The matrix effect was moderate with values ranging from −99.47% to 51.98%. Matrices procured from large markets tested negative for the 12 anthelmintics. The developed method proved amenable to real sample testing and can be used for simultaneous determination of target analytes in aquatic products.
研究领域:
分析化学
·
食品科学
·
兽医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2023
·
IF 5.2
Yunhang Liu,Qian Wang,Gan Zhu,Qiwen Ran,Fang Li,Meimei Guo,Guifang Wang,Hongyuan Zhao
We reported an one-pot ultrasonic-assisted method for the preparation of palygorskite nanorods/Super P Li carbon nanoparticles-graphitized carbon nanotubes (PNRs/SPCNPs-g-CNTs) nanocomposite, which was used to modify the glassy carbon electrode (GCE) for the fabrication of PNRs/SPCNPs-g-CNTs/GCE sensor. For the PNRs/SPCNPs-g-CNTs nanocomposite, PNRs with good stability presented large specific surface area and high adsorption, which promoted the enrichment of NA molecules on the electrode surface. SPCNPs with pearl chain-like nanostructure exhibited good electrical conductivity , and the combination of SPCNPs and g-CNTs with high graphitization degree formed an interconnected carbon conductive network with excellent electrical conductivity, which enhanced the charge transport efficiency. Moreover, the interconnected carbon conductive network of SPCNPs-g-CNTs not only promoted the dispersion degree of PNRs but also made up for the poor conductivity property of PNRs. When used for the detection of niclosamide (NA), an acceptable limit of detection (3.6 nM) was achieved at the PNRs/SPCNPs-g-CNTs/GCE sensor in linear NA concentration range of 0.01–10 μM. The PNRs/SPCNPs-g-CNTs/GCE sensor exhibited good reproducibility, repeatability, and anti-interference performance. For the practicability measurement, the fabricated sensor showed good practicability with satisfactory recoveries (97.0–102.7%) and low RSD values of 0.99–4.78% for the detection of NA in tap water and lake water samples.
研究领域:
分析化学
·
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Materials Research and Technology-JMR&T
·
2022
·
IF 6.267
Cuiling Wang,Fang Li,Jinna Li,Liusu Cui,Jiateng Zhong,Hongyuan Zhao,Sridhar Komarneni
A highly sensitive electrochemical sensor was successfully fabricated for the determination of niclosamide (NA) based on the chitosan (CTS) functionalized carbon nanotube@carbon black scaffolds (CNT@CBS) nanocomposite modified glassy carbon electrode (GCE). CNT@CBS with interconnected long- and short-range conductive network provided more efficient charge transfer channels and high electrochemical active surface area;CTS not only promoted the uniform dispersion of CNT@CBS but also possessed good biocompatibility, high adsorption capability, and excellent film-forming property. Benefitting from the synergistic effect of CNT@CBS and CTS, the CNT@CBS@CTS/GCE sensor showed a low limit of detection of 4.3 nM (Linear NA concentration: 0.01–10 μM). When applied for the NA detection in real samples, the fabricated sensor presented good practicability with satisfactory RSD values of 2.62%–4.35% and recoveries of 95.6–100.5%.
研究领域:
分析化学
·
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Wei Zhong,Jin Zou,Qi Yu,Yansha Gao,Fengli Qu,Shuwu Liu,Hui Zhou,Limin Lu
The development of effective electrochemical methods for the determination of pesticide residues is highly desirable for food safety requirements. Herein, a novel electrochemical sensing strategy for indirect detection of thiabendazole (TBZ) was achieved by monitoring the anodic stripping peak signal change of media Cu 2+ induced by a significant activity difference between active Cu 2+ and inactive Cu 2+ -TBZ complexes. In this sensing system, a heterostructured Ti 3 C 2 T x -TiO 2 composite synthesized via a simple in-situ-oxidization strategy is used as the electrode material to boost the anodic stripping peak signal. After optimizing various conditions, the developed sensor presents satisfactory analytical performance for TBZ assay with a linear range from 0.3 to 100.0 nM and a limit of detection as low as 0.1 nM (S/N = 3). Furthermore, the proposed sensing platform also exhibits outstanding anti-interference, repeatability, and stability, which is effective for the determination of TBZ in fruit and water samples.
研究领域:
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）