ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2017
·
IF 3.743
Xiong Pan,Saige Wang,Nan Shi,Hua Fang,Yunlong Yu
The application of the herbicide chlorimuron-ethyl has a lasting toxic effect on some succession crops. Here, a bacterium capable of utilizing chlorimuron-ethyl as the sole source of nitrogen was isolated from the contaminated soil and was identified as Enterobacter ludwigii sp. CE-1, and its detoxification and degradation of the herbicide were then examined. The biodegradation of chlorimuron-ethyl by the isolate CE-1 was significantly accelerated with increasing concentration (1–10 mg/l) and temperature (20–40 °C). The optimal pH for the degradation of chlorimuron-ethyl by the isolate CE-1 was pH 7.0. A pathway for the biodegradation of chlorimuron-ethyl by the isolate CE-1 was proposed, in which it could be first converted into 2-amino-4-chloro-6-methoxypyrimidine and an intermediate product by the cleavage of the sulfonylurea bridge and then transformed into saccharin via hydrolysis and amidation. The plant height and fresh weight of corn that had been incubated in nutrient solution containing 0.2 mg/l of chlorimuron-ethyl significantly recovered to 83.9% and 83.1% compared with those in the uninoculated control, although the root growth inhibition of chlorimuron-ethyl could not be alleviated after inoculation for 14 d. The results indicate that the isolate CE-1 is a promising bacterial resource for the biodegradation and detoxification of chlorimuron-ethyl.
研究领域:
环境科学
·
微生物学
·
植物生理学
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产品关联
DOI
PubMed
高影响因子
Nanomaterials
·
2018
·Vol. 8
·期 7
·499
·
IF 3.504
Guangyang Liu,Ruonan Zhang,Lingyun Li,Xiaodong Huang,Tengfei Li,Meng Lu,Donghui Xu,Jing Wang
Monitoring of low levels of chlorsulfuron in environmental water samples is important. Although several detection methods have been developed, they all have some drawbacks, such as being time-consuming, requiring expensive instruments and experienced operators, and consuming large volumes of organic solvents. There is an urgent need for a simple, rapid, and inexpensive detection method for chlorsulfuron. Herein, such a method was developed using anti-aggregation of gold nanoparticles (AuNPs) in the presence of acetamiprid in agricultural irrigation water samples. Aggregation of the AuNPs was induced by acetamiprid, and this produced a distinct color change from Bordeaux red to blue. However, the strong hydrogen bonding interaction between chlorsulfuron and acetamiprid could inhibit AuNP aggregation. The effect of chlorsulfuron on the anti-agglomeration behavior of AuNPs was monitored by ultraviolet–visiblespectroscopy (UV-Vis) and the naked eye over a concentration range 0.1–100 mg/L. The detection limit for chlorsulfuron was 0.025 mg/L (signal-to-noise ratio of three). This colorimetric method was successfully applied to the determination of chlorsulfuron in spiked tap water and agricultural irrigation water with satisfactory recoveries (76.3%–94.2%).
研究领域:
环境科学
·
分析化学
✓ 已验证
DOI
高影响因子
MOLECULES
·
2019
·Vol. 24
·期 17
·3060
·
IF 3.06
Shuang Gao,Yan-Yan Liu,Jing-Yu Jiang,Ying Fu,Li-Xia Zhao,Chun-Yan Li,Fei Ye
Herbicide safeners selectively protect crops from herbicide injury while maintaining the herbicidal effect on the target weed. To some extent, the detoxification of herbicides is related to the effect of herbicide safeners on the level and activity of herbicide target enzymes. In this work, the expression of the detoxifying enzyme glutathione S-transferase (GST) and antioxidant enzyme activities in maize seedlings were studied in the presence of three potential herbicide safeners: 3-dichloroacetyl oxazolidine and its two optical isomers. Further, the protective effect of chiral herbicide safeners on detoxifying chlorsulfuron in maize was evaluated. All safeners increased the expression levels of herbicide detoxifying enzymes, including GST, catalase (CAT), and peroxidase (POD) to reduce sulfonylurea herbicide phytotoxicity in maize seedlings. Our results indicate that theR-isomer of 3-(dichloroacetyl)-2,2,5-trimethyl-1,3-oxazolidine can induce glutathione (GSH) production, GST activity, and the ability of GST to react with the substrate 1-chloro-2,4-dinitrobenzene (CDNB) in maize, meaning that theR-isomer can protect maize from damage by chlorsulfuron. Information about antioxidative enzyme activity was obtained to determine the role of chiral safeners in overcoming the oxidative stress in maize attributed to herbicides. The interaction of safeners and active target sites of acetolactate synthase (ALS) was demonstrated by molecular docking modeling, which indicated that both isomers could form a good interaction with ALS. Our findings suggest that the detoxification mechanism of chiral safeners might involve the induction of the activity of herbicide detoxifying enzymes as well as the completion of the target active site between the safener and chlorsulfuron.Keywords:chiral safener;biological activity;antioxidative enzyme activity;protection mechanism;chlorsulfuron
研究领域:
植物生理学
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分子生物学
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农药科学
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DOI
PubMed
高影响因子
CANADIAN JOURNAL OF PLANT SCIENCE
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2015
·Vol. 95
·期 5
·
IF 0.919
ZhaoLi-Xia,QuHai-Tao,FuYing,GaoShuang,YeFei
Zhao, L.-X., Qu, H.-T., Fu, Y., Gao, S. and Ye, F. 2015. Alleviation of injury from chlorimuron-ethyl in maize treated with safener 3-dichloroacetyl oxazolidine. Can. J. Plant Sci. 95: 897–903. The protective effects of herbicide safeners, including 3-dichloroacetyl-2,2-dimethyl-1,3-oxazolidine (R-28725), 3-dichloroacetyl-2,2-dimethyl-4-ethyl-1,3-oxazolidine (Racemate), and its two enantiomers (R)-3-dichloroacetyl-2,2-dimethyl-4-ethyl-1,3-oxazolidine (R-enantiomer) and (S)-3-dichloroacetyl-2,2-dimethyl-4-ethyl-1,3-oxazolidine (S-enantiomer), on reducing the phytotoxicity of chlorimuron-ethyl to maize were investigated. Soaking the seeds in safeners increased the endogenous glutathione (GSH) content and glutathione-S-transferase (GST) activity of maize. When induced by R-enantiomer, the GST activity in vivo and in vitro increased 180 and 192% compared with control, respectively. R-28725 and R-enantiomer also increased the acetolactate synthase (ALS) activity inhibited by chlorimuron-ethyl from 45 to 100 and 97% compared with the control, respectively. The kinetic parameter Vmax of GST in the maize treated with R-28725 and R-enantiomer increased by 103 and 92%, respectively, compared with the control. Our results suggest that R-28725 and R-enantiomer could significantly improve the GSH content, GST activity, and ALS activity of maize. Overall, maize could be protected from the injury caused by chlorimuron-ethyl.
研究领域:
植物科学
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农业化学
✓ 已验证
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2022
·
IF 14.224
Qingyun Ma,Xiaoyan Han,Jinlong Song,Jie Wang,Qingqing Li,Rebecca E. Parales,Lin Li,Zhiyong Ruan
The widespread use of the herbicide chlorimuron-methyl is hazard to rotational crops and causes soil degradation problems. Biodegradation is considered a promising way for removing herbicide residues from the environment. Here, a new isolated strain, Cedecea sp . LAM2020, enabled complete degradation of 100 mg/L chlorimuron-methyl within five days. Transcriptome analysis revealed that ABC transporters, atrazine degradation and purine metabolism were enriched in the KEGG pathway. Integrating GO and KEGG classification with related reports, we predict that carboxylesterases are involved in the biodegradation of chlorimuron-methyl by LAM2020. Heterologous expression of the carboxylesterase gene carH showed 26.67% degradation of 50 mg/L chlorimuron-methyl within 6 h. The intracellular potential biological response and extracellular degradation process of chlorimuron-ethyl were analyzed by the nontarget metabolomic and mass spectrometry respectively, and the biodegradation characteristics and complete mineralization pathway was revealed. The cleavage of the sulfonylurea bridge and the ester bond achieved the first step in the degradation of chlorimuron-methyl. Together, these results reveal the presence of acidolysis and enzymatic degradation of chlorimuron-methyl by strain LAM2020. Hydroponic corn experiment showed that the addition of strain LAM2020 alleviated the toxic effects of chlorimuron-ethyl on the plants. Collectively, strain LAM2020 may be a promising microbial agent for plants chlorimuron-ethyl detoxification and soil biofertilizer .
研究领域:
环境微生物学
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生物修复
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植物科学
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产品关联
DOI
PubMed
高影响因子
Journal of the Taiwan Institute of Chemical Engineers
·
2022
·
IF 5.477
Ruilian Li,Yujiao Wen,Meng Liu,Lezhu Su,Yifan Wang,Shikai Li,Mei-e Zhong,Zhi Zhou,Nan Zhou
Background In this study, double modified biochar was prepared to remove organic matter and heavy metals at the same time. It has high removal efficiency for organic matter even in actual irrigation water. Methods Tobacco stem was used as carbon-based carrier, modified with polyacrylic acid (PAA) rich in oxygen functional groups (OFGs) and transition metals (iron and cobalt) with excellent catalytic activity. After mixing them evenly, the in-situ double modified biochar (BC PAA @CFO) of OFGs and CoFe 2 O 4 (CFO) was obtained by one-step hydrothermal carbonization (HTC). Significant findings BC PAA @CFO in QNC-Pb 2+ composite system, CFO can quickly activate peroxymonosulfate (PMS), and the degradation efficiency of quinclorac (QNC) is as high as 80.7%. Free radical quenching experiments and electron paramagnetic resonance (EPR) analysis indicated that SO 4 · − , ·OH, ·O 2 − and 1 O 2 were the main active species involved in the degradation of QNC. At the same time, the rich OFGs make the adsorption efficiency of Pb 2+ reach 73.2% within 4 h. FT-IR results further confirmed that the dominant role of adsorption should be OFGs. In general, this in-situ double modified BC PAA @CFO obtained by one pot HTC is a promising purifying agent for mixed pollutants.
研究领域:
环境科学
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水处理
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污染物去除
·
生物炭改性
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产品关联
DOI
高影响因子
JOURNAL OF ENVIRONMENTAL MANAGEMENT
·
2025
·
IF 8.4
Bingkun Liu,Zihao Zhang,Xinying Xie,Wen Zhang,Weijun Tian
Strategic engineering of heteroatomic doping configurations to precisely regulate the directional migration efficiency of photogenerated charge carriers has been demonstrated as a pivotal approach to overcome inherent kinetic limitations in semiconductor-based photocatalytic systems. Li/Co co-doped MoS 2 nanoflower structures (MLC2) were synthesized via a single-step hydrothermal protocol and applied for the photocatalytic degradation of chlorsulfuron in aqueous environments. Density functional theory analysis revealed that Li and Co atoms occupied Mo sites, generating electron-deficient centers that modified photogenerated electron transfer pathways. This strategic doping engineering enhanced charge separation efficiency, as evidenced by transient photocurrent response, electrochemical impedance spectroscopy, and Tafel curves. The optimized MLC2 catalyst demonstrated exceptional degradation performance, achieving 89.72 % chlorsulfuron removal within 360 min under visible light irradiation (λ ≥ 420 nm; 300 W), corresponding to quasi-first-order rate constants (k) 52.46-fold and 3.37-fold higher than those of undoped MoS 2 (k = 1.22 E −4 min −1 ) and Li-MoS 2 (k = 0.0019 min −1 ), respectively. The dominant role of photogenerated electrons, along with their generation and transfer pathways, had been experimentally validated. Furthermore, MLC2 demonstrated considerable potential in practical applications.
研究领域:
环境催化
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材料科学
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光催化
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半导体纳米材料
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水体净化
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2021
·
IF 2.694
Guangyu Xu,Fanyuan Zhang,Zhenghui Li
Cinosulfuron was dissolved in a series of cosolvent mixtures, which involved five pure solvents as raw materials and formed four sets of mixed solvents according to their pairs by mixing methanol, ethanol, n-propanol, and N,N-dimethyl formamide (DMF) in water. Taking 278.15 K as the starting temperature and referring to the principle of the isothermal static state, the temperature is gradually increased at 5 K intervals until it reaches 323.15 K. Under normal pressure (101.1 kPa), when the mole fraction of the solute did not change, the value obtained by HPLC was the equilibrium solubility of cinosulfuron. From the point of view of temperature, the influence on the solute cannot be ignored. The amount of cinosulfuron dissolved from a low value to a high value with the temperature from low to high. In addition, the difference was that the water content of the mixed solvent was repulsive to the dissolution of the solute. When the water content was from low to high, the mole fraction of cinosulfuron was from high to low. In any temperature environment, the same proportion of water was added to the four organic solvents. Compared with other solvents, the mixture of DMF and water had an absolute dissolution advantage. Besides, the Jouyban–Acree model, van’t Hoff–Jouyban–Acree model, and Apelblat–Jouyban–Acree model were used to correlate the solubility of cinosulfuron. The largest relative average deviation and root-mean-square deviation were 1.01 × 10–2 and 1.78 × 10–4 based on the correlation results, respectively. The acquisition of solubility helps in obtaining solubility parameters, which is a measure of intermolecular forces. Solubility is required in the fields of recrystallization and fractional crystallization in the chemical industry, as well as the synthesis and separation of compounds.
研究领域:
化学工程
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物理化学
·
药学
·
材料科学
✓ 已验证
DOI
高影响因子
MARINE ENVIRONMENTAL RESEARCH
·
2025
·
IF 3.2
Yufei Dai,Xianli Song,Jing Zhao,Limin Wang,Xiaoru Cui,Li Lu,Jingyu Zhang,Haoyu Zhang,Dahai Zhang,Keqiang Li
Pesticides occur widely in coastal waters and potentially pose a risk to marine ecosystems and human health. The spatiotemporal distribution of current-use pesticides (CUPs) was studied in Laizhou Bay (LZB) and the Xiaoqing River watershed in China during the summer of 2022 and spring of 2023. The pesticide composite index ( PCI ) and persistence index ( PI ) were established in association with the risk quotient ( RQ ) to assess the ecological risk in LZB. In this study, 17 and 12 kinds of CUP were detected in rivers and LZB seawater, respectively, with atrazine and neonicotinoid insecticides (NEOs) being the most frequently detected CUPs at higher concentrations. The average concentration of CUPs decreased from rivers to the coast and towards the center of the LZB, with higher levels in spring and lower levels in summer in rivers and a reversed pattern in seawater. The high-risk areas lie in the LZB coastal area and the estuary. Atrazine, chlorpyrifos, and imidacloprid might pose a high risk to aquatic organisms based on the RQ in the LZB, whereas the priority concern pesticides are atrazine and triazophos based on the PCI and PI . This study is of great significance for assessing the composite ecological risk of CUPs in aquatic environments.
研究领域:
环境科学
·
海洋生态学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Lu Zeng,Huan Lv,Xiangrui Li,Wenjia Yang,Wencan Luo,Linghong Kong,Qiujin Zhu
Cyclosulfamuron (CS) is a widely used sulfonylurea herbicide in plant cultivation and food production, and poses significant threats to ecological balance and food safety. To address this concern, we designed a novel CS hapten using computer-simulated semi-antigens. A monoclonal antibody (mAb) exhibiting high specificity and affinity was generated, with an affinity constant of 1.049×10 9 L/mol and a semi-inhibitory concentration (IC 50 ) of 4.94 ng/mL. Leveraging this anti-CS mAb, we constructed a rapid, visually detectable, quantitative lateral flow immunoassay (LFIA) to detect CS in cereals. Under the optimal conditions, the cut-off values were determined to be 100 μg/kg for brown rice and corn samples and 50 μg/kg for rice samples. The corresponding limits of detection values (LODs) were found to be 1.93 μg/kg in brown rice, 2.18 μg/kg in corn and 1.93 μg/kg in rice, respectively. The recovery rates ranged from 96.2% to 104.8% with coefficients of variation ranging from 3.5% to 6.4%, showing good agreement with the results obtained by liquid chromatography–tandem mass spectrometry (LC–MS/MS). The proposed assay demonstrated robust analytical performance, with significant application potential for rapid, on-site detection.
研究领域:
食品安全
·
分析化学
·
免疫学
✓ 已验证
产品关联
DOI
高影响因子
Nano Research
·
2024
·
IF 9.5
Lu Qianqian,Xu Xinxin,Qu Aihua,Liu Liqiang,Li Yuzhe,Sun Maozhong,Xu Chuanlai,Kuang Hua
Herbicide residues in agricultural products can have adverse effects on the environment and human health, therefore, there is an urgent need to establish a sensitive, rapid, and wide-ranging detection method. In this study, haptens of phenylurea herbicides (PUs) and sulfonylurea herbicides (SUs) were analyzed and designed based on computational simulation techniques, and two high-performance broad-spectrum monoclonal antibodies against PUs and SUs were prepared. On this basis, a multi-colloidal gold immunochromatography assay (multi-CGIA) was developed to simultaneously detect 13 herbicides in wheat. The visual limit of detection (vLOD) for PUs including diuron, chlortoluron, neburon, chlorbromuron, and linuron was 1–2 µg/kg. The vLOD for SUs including metsulfuron methyl, ethametsulfuron-methyl, sulfometuron-methyl, tribenuron methyl, cinosulfuron, triasulfuron, chlorimuron-ethyl, and chlorsulfuron was 2–10 µg/kg. The results of real sample determination indicated that the multi-CGIA is accurate, stable, and reliable, and adaptable to on-site preliminary screening of actual samples.
研究领域:
分析化学
·
环境科学
·
农业科学
✓ 已验证
产品关联
DOI
高影响因子
Food Analytical Methods
·
2019
·Vol. 12
·期 9
·1938-1948
·
IF 2.413
Liang Ting,Gao Lei,Qin Dongli,Chen Ligang
A method for determination of sulfonylurea herbicides (SUHs) in grain was developed by using mesoporous structured molecularly imprinted polymers (M-MIPs) as adsorbents of matrix solid-phase dispersion (MSPD) extraction, coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS). In this method, M-MIPs were synthesized by selecting chlorimuron-ethyl as template molecule, cetyltrimethylammonium bromide as mesoporous structure directing agent, 3-aminopropyltriethoxysilane as functional monomer, and tetraethyl orthosilicate as crosslinker. Then, M-MIPs were characterized by diverse instrumental techniques, absorption characteristics of M-MIPs were studied, and the experimental conditions for the MSPD extraction were optimized. MSPD enabled extraction and clean-up steps to be completed simultaneously. The M-MIPs with mesoporous structure improved detection selectivity, increased adsorptive capacity, and accelerated adsorption rate. Under the best conditions, excellent linearity of SUHs was obtained in the range of 0.25–25 μg kg −1 , the determination limits of chlorimuron-ethyl, nicosulfuron, thifensulfuron-methyl and chlorsulfuron were 0.075, 0.069, 0.083, and 0.105 μg kg −1 , respectively. The relative standard deviations were less than 5.5%.
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）