INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Yushi Wang,Mengjiao Xing,Lijia Duan,Aya Fatihi,Miao Ji,Danli Wang,Ling Li,Gongshuai Song,Tinglan Yuan,Jinyan Gong
In this study, complexes of chlorogenic acid (CA) with starch from different sources were prepared by thermal treatment (70 °C, 20 min) and ultrasound (450 W, 20 kHz, 25 °C, 20 min). Both ultrasound and thermal treatment are effective methods for preparing starch-CA complexes, but the two treatments have different effects on different starches. Characterization of the complexes prepared using the two methods showed that thermal treatment and ultrasound reduced the crystallinity of starch to some extent, with thermal treatment having a greater effect. Thermal treatment also reduced the amount of double-helix structures in starch. In contrast, ultrasound was relatively mild and had less effect on starch granular structure. However, these two methods promoted the binding of CA, thereby increasing the crystallinity of starch and forming a more ordered and compact structure, which, to some extent hindered the enzymatic degradation of starch. Moreover, the RDS of starch-CA complexes were reduced to different degrees, with the most significant changes in U (PS-CA) and T (CS-CA), which were reduced by 5.96 % and 4.55 %, respectively.
研究领域:
食品科学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
FOOD SCIENCE AND BIOTECHNOLOGY
·
2015
·Vol. 24
·期 3
·1131-1138
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IF 0.653
Wang Huaqian,Li Dongli,Du Zhiyun,Huang Mou-Tuan,Cui Xiaoxing,Lu Yujing,Li Chenyue,Woo Swee Lian,Conney Allan H.,Zheng Xi,Zhang Kun
Stems and leaves of Acanthopanax trifoliatus (L) Merr (AT) are commonly used as an edible vegetable in China. The scientific basis of antioxidant and antiinflammatory activities of AT was investigated. The polyphenol contents and free radical scavenging activities of AT ethanol extracts (EAT) were determined. Chlorogenic acid, isochlorogenic acid A, isochlorogenic acid C, and rutin were identified as major components in EAT. LPS-induced NO production in RAW264.7 cells was decreased significantly ( p <0.05) after EAT treatment, compared with controls. In vivo studies indicated that EAT had more activity than chlorogenic acid per mg of dry weight for inhibition of TPA-induced ear edema. TPA-induced increases in cytokine production (TNF-α, IL-6, MMP-9, and TIMP-1) were significantly ( p <0.05) reduced by EAT treatment, compared with controls. EAT exerts anti-inflammatory activities probably due, at least in part, to chlorogenic acid.
研究领域:
药理学
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植物化学
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产品关联
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2023
·
IF 5.8
Haibo Huang,Lihang Chen,Yiyang Hou,Wanying He,Xinchuang Wang,Dan Zhang,Jiangning Hu
Wound healing remains a considerable challenge due to its complex inflammatory microenvironment. Developing novel wound dressing materials with superior wound repair capabilities is highly required. However, conventional dressing hydrogels for wound healing are often limited by their complex cross-linking, high treatment costs, and drug-related side effects. In this study, we report a novel dressing hydrogel constructed only by the self-assembly of chlorogenic acid (CA). Molecular dynamic simulation studies revealed the formation of CA hydrogel was mainly through non-covalent interactions, such as π-π and hydrogen bond . Meanwhile, CA hydrogel exhibited superior self-healing, injectability, and biocompatibility properties, making it a promising candidate for wound treatment. As expected, in vitro experiments demonstrated that CA hydrogel possessed remarkable anti-inflammatory activity, and its ability to promote the generation of microvessels in HUVEC cells, as well as the promotion of microvessel formation in HUVEC cells and proliferation of HaCAT cells. Subsequent in vivo investigation further demonstrated that CA hydrogel accelerated wound healing in rats through regulating macrophage polarization. Mechanistically, the CA hydrogel treatment enhanced the closure rate, collagen deposition, and re-epithelialization while simultaneously suppressing the secretion of pro-inflammatory cytokines and increasing the production of CD31 and VEGF during the wound healing process. Our findings indicate that this multifunctional CA hydrogel is a promising candidate for wound healing, particularly in cases of impaired angiogenesis and inflammatory responses.
研究领域:
生物材料学
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医学
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药理学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2014
·
IF 3.259
Peng-Cheng Sun,Ying Liu,Yue-Tao Yi,Hong-Juan Li,Ping Fan,Chuan-Hai Xia
In the present study, a simple and efficient method for the preparative separation of 3-CQA from the extract of Helianthus tuberosus leaves with macroporous resins was studied. ADS-21 showed much higher adsorption capacity and better adsorption/desorption properties for 3-CQA among the tested resins. The adsorption of 3-CQA on ADS-21 resin at 25 °C was fitted best to the Langmuir isotherm model and pseudo-second-order kinetic model. Dynamic adsorption/desorption experiments were carried out in a glass column packed with ADS-21 to optimise the separation process of 3-CQA from H. tuberosus leaves extract. After one treatment with ADS-21, the content of 3-CQA in the product was increased 5.42-fold, from 12.0% to 65.2%, with a recovery yield of 89.4%. The results demonstrated that the method was suitable for large-scale separation and manufacture of 3-CQA from H. tuberosus leaves.
研究领域:
植物化学
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天然产物开发
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DOI
高影响因子
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
·
2014
·
IF 1.862
Demei Xu,Lihua Hu,Xiaomin Xia,Jianbo Song,Lingrui Li,Erqun Song,Yang Song
Tetrachlorobenzoquinone (TCBQ) is an active metabolite of pentachlorophenol (PCP). Although PCP has been investigated extensively, there are only a few reports describing the toxicity effect of TCBQ, and no report regarding TCBQ-induced liver injury in vivo . In the current study, we aimed to examine the acute hepatic toxicity of TCBQ in the mice model. Chlorogenic acid (CGA) exhibits promising antioxidant activity in the past studies, thus, the second aim of this study was to evaluate the protective effect of CGA on TCBQ-induced liver injury. Our results indicated TCBQ-intoxication caused marked liver cell necrosis and inflammation but not apoptosis, and this damage was alleviated by CGA treatment. Meantime, TCBQ-intoxication enhanced serum ALT, AST activities, TBIL content, hepatic oxidative stress and lipid peroxidation, decreased GSH content and inhibited the activities of antioxidant enzymes. Western blot and immunohistochemical analysis showed that TCBQ marked up-regulated HO-1 and NQO1 expression. On the other hand, pretreatment of CGA reduced TCBQ-induced liver damage remarkably. Taking together, these results revealed that TCBQ has strong hepatic toxic effect, and at least a part of this effect is initiated by free radical and relieved with CGA administration.
研究领域:
毒理学
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药理学
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分子生物学
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Xiaoqian Ding,Dequan Zhang,Huan Liu,Zhenyu Wang,Teng Hui
There are few studies on controlling the formation of heterocyclic amines (HAs) by adding polyphenols to roasted lamb. The aim of this study was to assess the inhibitory effect of 0.025, 0.125, and 0.625 mmol of chlorogenic acid, epicatechin, rutin, quercetin and quinic acid on the formation of HAs in charcoal roasted lamb, respectively, by UHPLC-MS/MS. The results indicated that Harman, Norharman, PhIP, IQx and 8-MeIQx were detected, but both chlorogenic acid and epicatechin greatly inhibited the formation of IQx, 8-MeIQx, Norharman, Harman and PhIP, and epicatechin had better inhibitory effect than chlorogenic acid. PLSR-VID analysis suggested the key precursors of HAs formation in roasted lamb were glucose, ribose, fructose, isoleucine, valine, and lysine. In addition, this work also implied that the potential mechanism of inhibition of HAs formation by chlorogenic acid and epicatechin in roasted lamb might attribute to competitive chemical reactions between polyphenols and key precursors of HAs.
研究领域:
食品科学
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生物化学
✓ 已验证
DOI
PubMed
高影响因子
Scientific Reports
·
2017
·Vol. 7
·期 1
·1-10
·
IF 4.259
Zhang Zhan,Wang Di,Qiao Shanlei,Wu Xinyue,Cao Shuyuan,Wang Li,Su Xiaojian,Li Lei
Hepatocellular carcinoma (HCC) treatment remains lack of effective chemopreventive agents, therefore it is very attractive and urgent to discover novel anti-HCC drugs. In the present study, the effects of chlorogenic acid (ChA) and caffeic acid (CaA) on HCC induced by diethylnitrosamine (DEN) were evaluated. ChA or CaA could reduce the histopathological changes and liver injury markers, such as alanine transarninase, aspartate aminotransferase, alkaline phosphatase, total bile acid, total cholesterol, high density lipoprotein cholesterol and low density lipoprotein cholesterol. The underlying mechanisms were investigated by a data integration strategy based on correlation analyses of metabonomics data and 16 S rRNA gene sequencing data. ChA or CaA could inhibit the increase of Rumincoccaceae UCG-004 and reduction of Lachnospiraceae incertae sedis , and Prevotella 9 in HCC rats. The principal component analysis and partial least squares discriminant analysis were applied to reveal the metabolic differences among these groups. 28 different metabolites showed a trend to return to normal in both CaA and ChA treatment. Among them, Bilirubin, L-Tyrosine, L-Methionine and Ethanolamine were correlated increased Rumincoccaceae UCG-004 and decreased of Lachnospiraceae incertae sedis and Prevotella 9 . These correlations could be identified as metabolic and microbial signatures of HCC onset and potential therapeutic targets.
研究领域:
医学
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药理学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Horticulture Research
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2021
·
IF 6.793
Liu Qun,Li Li,Cheng Haitao,Yao Lixiang,Wu Jie,Huang Hui,Ning Wei,Kai Guoyin
Polyphenols are the main active components of the anti-inflammatory compounds in dandelion, and chlorogenic acid (CGA) is one of the primary polyphenols. However, the molecular mechanism underlying the transcriptional regulation of CGA biosynthesis remains unclear. Hydroxycinnamoyl-CoA:quinate hydroxycinnamoyl transferase (HQT2) is the last rate-limiting enzyme in chlorogenic acid biosynthesis in Taraxacum antungense. Therefore, using the TaHQT2 gene promoter as a probe, a yeast one-hybrid library was performed, and a basic helix-loop-helix (bHLH) transcription factor, TabHLH1, was identified that shared substantial homology with Gynura bicolor DC bHLH1. The TabHLH1 transcript was highly induced by salt stress, and the TabHLH1 protein was localized in the nucleus. CGA and luteolin concentrations in TabHLH1-overexpression transgenic lines were significantly higher than those in the wild type, while CGA and luteolin concentrations in TabHLH1-RNA interference (RNAi) transgenic lines were significantly lower. Quantitative real-time polymerase chain reaction demonstrated that overexpression and RNAi of TabHLH1 in T. antungense significantly affected CGA and luteolin concentrations by upregulating or downregulating CGA and luteolin biosynthesis pathway genes, especially TaHQT2, 4-coumarate-CoA ligase (Ta4CL), chalcone isomerase (TaCHI), and flavonoid-3′-hydroxylase (TaF3′H). Dual-luciferase, yeast one-hybrid, and electrophoretic mobility shift assays indicated that TabHLH1 directly bound to the bHLH-binding motifs of proTaHQT2 and proTa4CL. This study suggests that TabHLH1 participates in the regulatory network of CGA and luteolin biosynthesis in T. antungense and might be useful for metabolic engineering to promote plant polyphenol biosynthesis.
研究领域:
植物生理学
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分子生物学
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转录组学
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产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2024
·Vol. 14
·期 1
·1-7
·
IF 3.8
Wang Xin,Gong Xianyun,Lin Binbin
Chlorogenic acid (CA) is an effective ingredient that can strengthen immunity during following the COVID-19 era. The current cost of CA is high owing to its complex purification process and low yield (approximately 2%). In this study, a one-step path orthogonal experiment was designed based on the results from Gauss calculation, which consisted of acidity, coordination, and hydrolysis in molecules. The optimized extraction conditions were 60 ℃, 60 min, 1:20 liquid ratio, and 40% ethanol in a nitrogen atmosphere controlled using a device of our own design, which led to CA yields of up to 6.35% from potato leaves. The purified CA was analyzed using high-performance liquid chromatography, thin-layer chromatography, ultraviolet–visible spectroscopy, and molecular fluorescence. This accurate and reproducible method can not only be used to obtain high yields of CA but can also be used for the quality control of active plant products and their isomers.
研究领域:
植物化学
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药用植物学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Yong Chen,Haifeng Zhang,Guosheng Wei,Fengsong Liu,Yue Zhang,Ying Chen
The broad application of starch films has been significantly limited by their insufficient hydrophobicity and antibacterial activity. To overcome these challenges, this study developed a new starch film by incorporating polyvinyl alcohol (PVA) and chlorogenic acid. The study explored the impact of PVA polymerization on the physical and functional characteristics of the resulting films, with particular emphasis on enhancing antimicrobial functionality by incorporating chlorogenic acid. Scanning electron microscopy (SEM) and rheological tests demonstrated the excellent compatibility and exceptional film-forming performance. Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analyses validated the presence of intermolecular entanglements and hydrogen bonding within the films. Incorporating PVA with a polymerization degree of 2400 resulted in a contact angle (CA) of 79.81 ± 1.74°, a water absorption capacity (WAC) of 10.29 ± 0.71 %, and a water vapor permeability (WVP) of (0.44 ± 0.11) × 10 −11 g × m −1 × s −1 × Pa −1 . Notably, the SCP 2488 film exhibited superior mechanical properties, including the highest Young's modulus of 71.29 MPa, tensile strength of 11.77 MPa, and elongation at break of 106.75 %. Additionally, such a modified film displayed enhanced UV-blocking performance and antibacterial efficacy. Consequently, the SCP 2488 film showed great potential for maintaining the freshness and quality of strawberries.
研究领域:
食品科学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2021
·
IF 9.147
Yuxue Zheng,Xiuxiu Yin,Xiangli Kong,Shiguo Chen,Enbo Xu,Donghong Liu,Yukiharu Ogawa,Xingqian Ye,Jinhu Tian
To investigate how chlorogenic acid (CHA) affects the physicochemical properties and hydrolysis of rice flour during extrusion. CHA was extruded with rice flour through a twin-screw extruder at different concentrations (0–2.0%), and the physicochemical properties of the extrudates, including crystallinity, pasting properties and CHA binding, were characterized through X-ray diffraction, Fourier-transform infrared spectroscopy, Rapid Visco Analyzer and ultraviolet spectrophotometer. Particularly, starch hydrolysis and the release of CHA were also evaluated through enzymatic hydrolysis and high-performance liquid chromatography. Results indicated that the addition of CHA promoted the formation of V-type crystals and increased the orderliness of the complexes. The maximum binding amount of CHA in extrudates was 10.98 mg/g without any isomerization or degradation. Additionally, the rapidly digestive starch (RDS) decreased markedly (down to 41.28 ± 2.66%), and there was a pronounced increase in resistant starch (RS), which varied from 10.28 ± 2.62 to 31.16 ± 0.50% in different extrudates. Our results indicated that CHA yielded a negative effect on starch hydrolysis and might provide basic guidance for the application of CHA to healthy starchy food processing in the future.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2025
·
IF 12.5
Meng Zhang,Yinchuan Wang,Xueling Yin,Mei Xue,Xin Zhao,Runxiao Zheng,Jianfeng Qiu,Zhihong Zhu
Developing safe and effective novel wound dressings to enhance full-thickness skin wound regeneration is highly desirable. In this study, we firstly incorporated chlorogenic acid (CA) into dopamine (DA) functionalized alginate (Alg-DA) conjugates and utilized polyvinyl alcohol (PVA) as the carrier polymer to fabricate a series of novel multifunctional composite nanofiber membranes (PVA/Alg-DA/CA) for promoting wound healing. These nanofiber membranes exhibited high water absorption, water vapor transmission rate, porosity, and hydrophilicity properties. The CA endowed the PVA/Alg-DA/CA membranes with excellent antibacterial properties, and the superior antioxidant activity to effectively protect cells from oxidative damage. Meanwhile, capitalizing on the unique nanofiber architecture, as well as the inherent biofunctional activities of CA and Alg-DA, these membranes exhibited remarkable biocompatibility, fostering a conducive environment for fibroblast adhesion and proliferation. Moreover, wound healing assessments and histopathological analyses revealed that composite membranes could promote neovascularization and tissue remodeling, and thus accelerating wound closure in the mouse full-thickness wound defect model. Additionally, the upregulation of key healing markers including CD31 and TGF-β1 protein expressions, further corroborated the ability of multifunctional membrane to stimulate the wound healing cascade. This multifunctional membranes with biosafety and therapeutic outcomes are a promising candidate for wound dressing to promote skin repair.
研究领域:
生物材料学
·
医学
·
药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）