PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2021
·
IF 3.963
Bao Dong,Xi-Ya Liu,Bin Li,Mao-Ye Li,Shi-Guang Li,Su Liu
Lambda-cyhalothrin (LCT) is a pyrethroid insecticide widely used to control insect pests . Insect exposure to LCT may cause abnormal accumulation of reactive oxygen species (ROS) and result in oxidative damage. Heat shock proteins (HSPs) may help protect against oxidative stress . However, little is known about the role of HSPs in response to LCT in the green peach aphid , Myzus persicae . This insect is an important agricultural pest causing severe yield losses in crops. In this study, we characterized a cDNA sequence ( MpHsp70 ) encoding a member of the HSP70 family in M. persicae . MpHsp70 encoded a 623 amino acid protein putatively localized in the cytosol. The highest expression level of MpHsp70 occurred in fourth-instar nymphs. Treatment of M. persicae with LCT resulted in oxidative stress and significantly increased H 2 O 2 and malondialdehyde levels. This led to an elevated transcription level of MpHsp70 . Injection of H 2 O 2 into M. persicae also upregulated the MpHsp70 expression level, suggesting that MpHsp70 is responsive to ROS, particularly H 2 O 2 , induced by LCT. Recombinant MpHSP70 protein was expressed in Escherichia coli . E. coli cells overexpressing MpHSP70 exhibited significant tolerance to H 2 O 2 and the ROS generators, cumene hydroperoxide and paraquat . This indicated that MpHSP70 protects against oxidative stress. Furthermore, knockdown of MpHsp70 by RNA interference resulted in increased susceptibility in apterous adults of M. persicae to LCT. These findings indicate that MpHsp70 plays an important role in defense against LCT-induced oxidative stress and insecticide susceptibility in M. persicae .
研究领域:
昆虫学
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分子生物学
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毒理学
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Zengting Niu,Guotong Sun,Mingtao Tan,Shanchun Yan,Dun Jiang
Heavy metals are prevalent environmental contaminants in pest habitats. This study investigates the tolerance of Hyphantria cunea larvae to β-cypermethrin under cadmium (Cd) stress. Exposure to Cd markedly enhanced larval tolerance to β-cypermethrin. The P450 enzyme was found to be crucial in triggering this cross-tolerance. Notably, Cd exposure significantly upregulated the expression of HcCYP333A29 and HcCYP6AE184 both before and after β-cypermethrin treatment. Silencing these genes reduced the β-cypermethrin tolerance in Cd-exposed larvae. Furthermore, overexpression of HcCYP333A29 and HcCYP6AE184 significantly improved the tolerance of Sf9 cells and Drosophila to β-cypermethrin. Silencing of HcDicer-1, a key gene in the miRNA synthesis pathway, significantly diminished the β-cypermethrin tolerance in Cd-treated larvae. The miR-2739 controlled overexpression of HcCYP333A29, while miR-2854 controlled HcCYP6AE184 overexpression, both of which were essential for β-cypermethrin cross-tolerance in H. cunea larvae under Cd exposure. Collectively, Cd exposure induces cross-tolerance to β-cypermethrin in H. cunea larvae through miRNA-mediated post-transcriptional regulation.
研究领域:
环境毒理学
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分子生物学
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昆虫学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2019
·
IF 5.714
Xueke Liu,Chang Liu,Peng Wang,Yiran Liang,Jing Zhan,Zhiqiang Zhou,Donghui Liu
Alpha-cypermethrin (Alpha-CP), an important pyrethroid pesticide , has been widely used for pest control in agriculture and parasite control in livestock farms. Thus, alpha-CP is easily exposed to wild birds and poultry, which may pose a potential risk to birds. Alpha-CP and its metabolites have been detected in many environmental samples, including poultry and wild birds. We studied the distribution and metabolism of alpha-CP and its metabolites in embryo development and newborn chick. The results showed that metabolites were the main residual forms of alpha-CP in different stages of life and might increase the exposure risk of bird and its offspring . Metabolomics investigation of newborn chick exhibited that the metabolic profiles of chicks were disturbed, especially lipid metabolism . The concentrations of cis-DCCA and trans-DCCA were high in the first and second weeks of chick growth, indicating that chicks have limited ability to further metabolize and excrete cis-DCCA and trans-DCCA during the early stages of chicks. Toxicokinetics of alpha-CP in adult hens showed that alpha-CP was rapidly metabolized to acid metabolites, which could be further metabolized and excreted. The results about metabolism of alpha-CP in different stages of chicken indicate that the ability of the embryo and early chick to metabolize alpha-CP and its metabolites was the weakest. Therefore, it is of important significance to focus on evaluating the ecological risk of cypermethrin on birds at different stages of life cycle.
研究领域:
毒理学
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生态学
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动物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Jian Zhu,Yunyan Lai,Yinhua Wu,Jiali Wang,Jiaqi Wei,Hanhong Xu
We screened the contact activity of 32 commercial essential oils (EOs) and their synergistic effect with β-cypermethrin against Blattella germanica. Results showed that the most effective EOs against B. germanica were from Illicium verum, Syzygium aromaticum, and Cinnamomum camphora, with LD50 values of less than 500 μg/insect. The most potent synergistic effects of β-cypermethrin on B. germanica were from Dysphania ambrosioides and Mentha canadensis. Both oils have a co-toxic factor of 133.33. The results of the major compound testing of the EOs showed that trans-anisaldehyde and thymol have the best insecticidal activity against B. germanica, with LD50 values of 141.30 and 138.61 μg/insect, respectively. The compounds with the best synergistic effect on β-cypermethrin were γ-terpinene and linalool at a concentration of 0.5%. The co-toxic factors for γ-terpinene and linalool were 150 and 133.33, respectively, which were similar to the synergistic effect observed with 2% piperonyl butoxide.
研究领域:
昆虫学
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植物化学
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害虫管理
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2015
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IF 3.391
Xueke Liu,Zhigang Shen,Peng Wang,Chang Liu,Guojun Yao,Jiayuan He,Donghui Liu,Zhiqiang Zhou
Isomerization of chiral pesticides in residue analysis has not drawn much attention which can cause wrong decisions on the enantioselective environmental behavior. A residue analysis method for α-cypermethrin and its three acid metabolites, cis/trans-3-(2,2-dichlorovinyl)-2,2-dimethyl cyclopropanecarboxylic acid (cis/trans-DCVA) and 3-phenoxybenzoic acid (3-PBA), in foods such as chicken, honey, milk and pork has been established, in which the isomerization of α-cypermethrin was minimized. The target compounds were determined by GC–ECD after a derivatization reaction with 1,1,1,3,3,3-hexafluoroisopropanol. The conditions of the method were optimized by Taguchi orthogonal experiment. Under the optimized conditions, good linearity was obtained with correlation coefficients ranging from 0.9964 to 0.9998. The limits of detection were 0.001–0.005 mg kg −1 . The recoveries were 70.9–114.9% with relative standard deviations of 0.6–15%. In addition, ratios of isomerization ranged only from 2.3% to 7.0%. This method was applied in commercial animal food products.
研究领域:
分析化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2021
·Vol. 28
·期 34
·47099-47106
·
IF 4.223
Jiang Wenqi,Yao Guojun,Jing Xu,Liu Xueke,Liu Donghui,Zhou Zhiqiang
Heavy metals may coexist with pesticides in farmland through wastewater irrigation, application of pesticides and chemical fertilizers, or unappropriated waste disposal. Heavy metals are toxic to soil microorganism, which may influence the environmental behavior of pesticides subsequently. In this study, the influence of Cd 2+ and Pb 2+ on the degradation of α-cypermethrin and its metabolites, 3-phenoxphenoxybenzoic acid (3-PBA) and 3-(2’,2’-dichlorovinyl)-2,2-dimethylcyclopropane carboxylic acid (DCCA), were investigated through soil incubation experiment. It was found heavy metals like Cd 2+ and Pb 2+ will inhibit the degradation of α-cypermethrin, especially at high concentrations. Pb 2+ has a stronger inhibitory effect on the degradation of α-cypermethrin than Cd 2+ in the same concentration. With the presence of 10 mg/kg Pb 2+ , the half-life of α-cypermethrin increased from 41.1 to 99.9 days, even the half-life was 129.3 days with 50 mg/kg of Pb 2+ . Besides, heavy metals influenced the chiral selective degradation of α-cypermethrin. The enantiomer fraction was near 0.5 when 10 mg/kg of heavy metals existed. Furthermore, the adverse effects of heavy metals on soil urease, catalase, and sucrase activity were assayed. In tested concentrations (10 and 50 mg kg -1 ), the heavy metals result in strong inhibition of the activity of the enzymes present on soil, jeopardizing the biodegradation by the microbiome and which may inhibit the degradation of α-cypermethrin.
研究领域:
环境科学
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土壤微生物学
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产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2019
·Vol. 76
·期 3
·1039-1047
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IF 3.255
Chao Hu,Wei Wang,Di Ju,Gao-Man Chen,Xiao-Ling Tan,David Mota-Sanchez,Xue-Qing Yang
BACKGROUND Recent work has shown that two codling moth ( Cydia pomonella ) glutathione S -transferase genes (GSTs), CpGSTd1 and CpGSTd3 , can metabolize λ-cyhalothrin, one of the recommended insecticides for C. pomonella control worldwide. However, systematical characterization of delta and epsilon GSTs, especially their potential contributions in the metabolism of λ-cyhalothrin, is currently still lacking in C. pomonella . RESULTS In this study, a total of nine cDNA sequences were identified in C. pomonella , including four in the delta and five in the epsilon subclasses. RT-qPCR showed that seven GSTs were ubiquitously expressed at all developmental stages, and CpGSTe2 , CpGSTe3 , and CpGSTe4 were mainly expressed in larvae. The mRNA levels of CpGSTd2 , CpGSTd4 , and CpGSTe5 were significantly higher in male than in female adults. Tissue-specific expression analysis revealed that the CpGSTe2 , CpGSTe3 , and CpGSTe4 were highly expressed in the midgut while CpGSTd2 and CpGSTd4 were predominantly expressed in the Malpighian tubules. The transcripts of these GSTs (except CpGSTe1 ) were co-expressed following exposure to LD 10 of λ-cyhalothrin for 3 h. Recombinant CpGSTd4, CpGSTe2, and CpGSTe3 proteins expressed in Escherichia coli displayed glutathione-conjugating activity toward 1-chloro-2,4-dinitrobenzene. In addition, λ-cyhalothrin could inhibit the activity of recombinant CpGSTd4, CpGSTe2, and CpGSTe3 enzymes, but only recombinant CpGSTe3 showed λ-cyhalothrin metabolic capacity, with 21.88 ± 1.09% of parental compound being depleted within 1 h. CONCLUSION These data show that CpGSTe3 is a third GST gene, encoding an enzyme that metabolizes λ-cyhalothrin in C. pomonella . © 2019 Society of Chemical Industry
研究领域:
昆虫学
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分子生物学
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农药科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
·
IF 6.1
Yu-Xi Liu,Chao Hu,Yu-Ting Li,Ping Gao,Xue-Qing Yang
While previous studies have reported G protein-coupled receptor (GPCR)-mediated insecticide resistance in various arthropods, the understanding of GPCR-associated resistance mechanisms in Cydia pomonella remains limited. In this study, a total of 95 CpGPCR genes categorized into four families were identified in C. pomonella. Results revealed high expression levels of the majority of the CpGPCRs during the first larval stage and in the head of C. pomonella. Exposure to lambda-cyhalothrin significantly increased the expression of 15 CpGPCRs, including CpGPCR70, which is highly expressed in all larval stages and shows the highest expression in the midgut. RNA interference (RNAi) demonstrated that downregulation of CpGPCR70 leads to reduced expression of key resistance-related genes and a decreased tolerance of larvae to lambda-cyhalothrin. These findings indicate that CpGPCR70 plays a crucial role in regulating the expression of detoxifying genes involved in lambda-cyhalothrin resistance, offering valuable insights for the development of more effective pest control strategies.
研究领域:
昆虫学
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分子生物学
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害虫防治
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产品关联
DOI
PubMed
高影响因子
Biosensors-Basel
·
2023
·Vol. 13
·期 3
·309
·
IF 5.4
Jingyang Zhu,Xinru Yin,Weiyi Zhang,Meilian Chen,Dongsheng Feng,Yong Zhao,Yongheng Zhu
Chlorpyrifos (CHL), profenofos (PRO) and cypermethrin (CYP) are widely used in combination to increase crop yields. However, these three pesticides can cause serious harm to human health and do not easily degrade. In this study, a novel visible paper sensor has been prepared successfully and different colorimetric reactions were utilized to detect the three pesticides simultaneously. The sensor was constructed by grafting a zwitterionic polymer onto a cellulose filter (CF) and placing it on a glass surface modified with PDMS. The branch shape was designed to form multiple detection areas, which were modified with specific pesticides and corresponding chromogenic reagents. The as-prepared colorimetric platform exhibited high sensitivity, a short detection time, a good linear response and a low detection limit (LOD) for the three pesticides (chlorpyrifos: y = 46.801 − 1.939x, R2= 0.983, LOD = 0.235 mg/L; profenofos: y = 40.068 + 42.5x, R2= 0.988, LOD = 4.891 mg/L; cypermethrin: y = 51.993 + 1.474x, R2= 0.993, LOD = 4.053 mg/L). The comparison of the results obtained by the proposed paper sensor and those obtained by spectrophotometry further revealed the stability and reliability of the paper sensor. In particular, the color intensity of the interaction between the pesticides and coloring agents could be directly observed by the human eye. The consistency of the colorimetric/optical assay was proven in real target pesticide samples. Thus, this sensing strategy provides a portable, cost-effective, accurate and visualized paper platform, which could be suitable for application in the fruit and vegetable industry for monitoring CHL, PRO and CYP in parallel.Keywords:microfluidic paper-based analytical devices;detection;chlorpyrifos;profenofos;cypermethrin
研究领域:
分析化学
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环境科学
·
农业科学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Yahui He,Yajie Liu,Xinyu Liu,Yao Zhu,Yaling Yang,Jing Wang,Baoguo Sun
Due to its common use as an insecticide during agricultural production, residual lambda-cyhalothrin (LC) in food and the environment presents a significant human health risk. Developing an accurate LC detection method that does not require pretreatment remains a challenge. This study developed a novel yellow carbon dots molecularly imprinted (YCDs@MIPs) polyvinylidene fluoride (PVDF) composite (YCDs@MIPs@PVDF) fluorescent membrane for rapid visual LC detection. This method required no pretreatments during analysis since the LC could be specifically adsorbed when the samples passed through the YCDs@MIPs@PVDF membrane, causing fluorescence quenching, while matrix-interfering components could be removed for the porous structure of the membrane. A smartphone was used for rapid visual LC detection based on membrane fluorescence color changes in a dark-box ultraviolet analyzer. In food with a 15 % PVDF content and 6 mL added YCDs@MIPs, LC was quantitatively detected in a broad concentration range (5–500 μg/L) with a limit of detection (LOD) of 0.039 μg/L to 0.16 μg/L, which was lower than the strictest EU2019/92 limit of 10 μg/L. The analysis time was reduced from over 8 h when using the GB 23200.121–2021 and GB/T20769–2008 national standard methods to 30 min, while the analytical procedure decreased from six steps to two. The visual detection strategy combining the YCDs@MIPs@PVDF composite fluorescent membrane with a smartphone provided a new rapid detection technique for composite membrane purification and fluorescence signal output function.
研究领域:
食品安全
·
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
Food Science and Human Wellness
·
2020
·
IF 2.455
Chengwei Yu,Xu Huang,Yawei Fan,Zeyuan Deng
Pyrethroid is widely used in developing countries and is a potential threat to human health. So putting forward an effective pyrethroid cleaning method is of vital importance. Clearance of fenpropathrin, cypermethrin and deltamethrin in cabbage by using ultrasonic treatment accompanied with different baking soda concentrations were investigated in this study. The results of response surface methodology showed that the maximum clearance rate was 70.61% for fenpropathrin under the optimal power, treated time, treated temperature and baking soda concentration, which were 260.02 W, 10 min, 20.15 °C and 0.014 g/mL, respectively. Similarly, the maximum removal efficiency was 73.72% for cypermethrin under 240 W, 13.84 min, 19.53 °C and 0.014 g/mL, and 92.15% for deltamethrin under 238.99 W, 12.42 min, 25 °C and 0.013 g/mL. The clearance rate of pyrethroid by this method was significant higher ( P < 0.05) than traditional cleaning methods (bathing and running water washing). The contents of the compositions such as vitamin C, total soluble sugar, protein and dietary fiber, texture and sensory evaluation of cabbage were not found obvious changes after the ultrasonic treatment combined with baking soda ( P > 0.05). Results showed that ultrasonic treatment combined with baking soda was an effective way to eliminate the fenpropathrin, cypermethrin and deltamethrin in cabbage without influencing cabbage quality.
研究领域:
食品安全
·
农业科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
·
2022
·
IF 6.76
Jiexiang Liu,Ruili Bai,Xiaoguang Zhang
The SB (DTAB, TTAB, CTAB, OTAB)-AP complex was obtained by modifying attapulgite (AP) with surfactant of SB (DTAB, TTAB, CTAB, OTAB). The structures were characterized by XRD, FT-IR, TGA/DTA and SEM. A new lamellar structure is formed by introducing surfactant. The basal spacing increases with the increase of alkyl chain length of cationic surfactants . Then, the beta-cypermethrin (BCT) composites of BCT/SB-AP, BCT/DTAB (TTAB, CTAB, OTAB)-SB-AP were prepared and investigated. The basal spacings of BCT composites were larger than those of SB-AP and the corresponding DTAB (TTAB, CTAB, OTAB)-AP. The existence of BCT and surfactants molecules into BCT composites was further confirmed. The BCT/DTAB (TTAB, CTAB, OTAB)-SB-AP have the smaller specific surface areas than DTAB (TTAB, CTAB, OTAB)-AP due to the increase of AP exfoliation in the presence of SB. Fabrication mechanism of BCT composites was put forward. Release behaviors of BCT were investigated and analyzed in different buffer solutions. The existence of mixed-surfactants influenced distinctly the release rates. BCT composites had the slow release property. The release behaviors were well fitted by pseudo second-order and parabolic diffusion models. This study provided a new approach to improve the utilization efficiency of pesticide and could be applied as a pesticide delivery system.
研究领域:
材料科学
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农业科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）