Agronomy-Basel
·
2022
·Vol. 12
·期 11
·2724
·
IF 3.949
Qian Yang,Xia Yang,Jinlei Zhu,Tian Wei,Min Lv,Yongfeng Li
A population ofEchinochloa crus-galli(L.) P. Beauv obtained from direct-seeding rice fields in Jiangxi Province, China, exhibited high resistance levels (13.5-fold) to the acetyl-CoA carboxylase (ACCase)-inhibiting herbicide cyhalofop-butyl. Compared with the susceptible (S) population, this resistant (R) population evolved a cross-resistance to aryloxyphenoxypropionates (APPs) herbicides metamifop (2.9-fold) and fenoxapro-p-ethyl (4.1-fold), cyclohexanediones (CHDs) herbicide clethodim (4.7-fold), phenyl pyrazoline (DEN) herbicide pinoxaden (6.4-fold), and evolved multiple-resistance to acetolactate synthase (ALS)-inhibiting herbicide penoxsulam (3.6-fold), and auxin mimic herbicides quinclorac (>34.7-fold) and florpyrauxifen-benzyl (2.4-fold).ACCasegene sequencing did not reveal the existence of any known mutation point conferring with herbicide resistance. In addition, three metabolic inhibitors—one glutathione—S-transferase (GST) inhibitor (NBD-Cl), and two cytochrome P450 inhibitors (malathion and PBO)—did not reverse the cyhalofop-butyl resistance. Furthermore, enhanced metabolic rates of more than 60% 24 h after treatment with the active compound cyhalofop acid was observed in R plants compared to S plants. Hence, enhanced metabolism activity endows a non-target-site resistance to cyhalofop-butyl in the R population ofE. crus-galli. Future research will be required to determine what metabolizing enzyme genes are responsible for cyhalofop-butyl resistance inE. crus-galli.Keywords:resistance mechanisms;metabolism;cyhalofop-butyl;Echinochloa crus-galli;resistance pattern;multiple-resistance
研究领域:
植物学
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遗传学
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农学
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DOI
高影响因子
JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY
·
2024
·
IF 1.3
Liang Limei,Wu Minghui,Huang Pengmian,Liu Jiyong
This work is based on the precursor compound cyproflanilide and designs a series of methoxy containing meta-diamine compounds. By replacing the fluorine atom on the phenyl ring in cyproflanilide with methoxy group, 17 derivatives (12a-12q) were obtained with yields ranging from 46 to 92%, which were characterized by high-resolution mass spectrometry, and nuclear magnetic resonance (1H and 13C) spectroscopy and infrared. The insecticidal activities were evaluated against the Plutella xylostella and Spodoptera frugiperda. Compound N-(3-((2-bromopropane-4-(perfluoropropan-2-yl))-6-(trifluoromethyl)phenyl)methoxyphenyl)-N-(cyclopropylmethyl)-6-fluoronicotinamide (12q) showed significant insecticidal activity against the Plutella xylostella with a mortality rate of 97.67% at a concentration of 1 mg L-1. It also exhibited moderate activity against the Spodoptera frugiperda at a concentration of 0.1 mg L-1, which can be used as a template for structure modification to obtain higher insecticidal activity.
研究领域:
农药化学
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有机合成
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农业昆虫学
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DOI
高影响因子
JOURNAL OF ECONOMIC ENTOMOLOGY
·
2016
·Vol. 110
·期 1
·230-238
·
IF 1.609
Xu Chunmei,Ding Jinfeng,Zhao Yunhe,Luo Jian,Mu Wei,Zhang Zhengqun
To better understand the application prospect of cyantraniliprole against the Asian corn borer, Ostrinia furnacalis, a diet-incorporation bioassay was adopted to determine the toxicity of cyantraniliprole against the fourth instar of O. furnacalis. Moreover, other experiments were conducted to examine effects of sublethal levels of cyantraniliprole on larval development, nutrient utilization, and reproduction. In this study, cyantraniliprole showed a high toxicity to fourth-instar larvae of O. furnacalis at dosages of 0.05 μg/g (LC5), 0.11 μg/g (LC20), 0.20 μg/g (LC40), and 0.26 μg/g (LC50) administered through artificial diet. At three sublethal dosages, cyantraniliprole inhibited larval feeding processes, decreased the relative growth rate, the relative consumption rate, the efficiency of food ingestion, and the efficiency of food digestion, as well as decreased the contents of nutrients such as proteins, lipids, and carbohydrates. All these effects go against to the normal growth of O. furnacalis, including reductions in larval and pupal weight, the extension of the larval and pupal period, the prolongation of the adult preoviposition period, total preoviposition period, and mean generation time, and the reduction of adult longevity, oviposition period, and eggs laid by female adults. Together, these changes resulted in the reduction of the intrinsic rate of increase. Hence, even at sublethal concentrations, cyantraniliprole can affect population dynamics by reducing the survival rate, fecundity, and population parameters. This result provides useful information for developing control strategies for O. furnacalis.
研究领域:
昆虫学
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害虫管理
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农业科学
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DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2024
·
IF 3.8
Minghao Jiang,Xumiao Wang,Wei Hu,Zezhou Wang,Huaiji Guan,Ning Zhao,Min Liao,Haiqun Cao
BACKGROUND Chinese sprangletop [ Leptochloa chinensis (L.) Nees] control is threatened by resistance to acetyl-CoA carboxylase (ACCase)-inhibiting herbicides. In this study, a L. chinensis population, HFLJ18, that survived cyhalofop-butyl [aryloxyphenoxypropionate (APP) herbicide, CyB] treatment was collected from a rice field in Lujiang County, Anhui Province, China. This study aimed to evaluate the susceptibility of HFLJ18 to herbicides with different modes-of-action and investigate the potential mechanisms of resistance to CyB. RESULTS The HFLJ18 population exhibited high levels of resistance to CyB (10.92-fold) and showed resistance to the ACCase inhibitors metamifop (4.63-fold) and fenoxaprop- P -ethyl (8.39-fold), but was susceptible to clethodim, pinoxaden, florpyrauxifen-benzyl, oxadiazon and pretilachlor. Target gene sequencing revealed a novel Trp-to-Gly substitution at codon position 2027 of ACCase in the resistant plants. Molecular docking revealed that the spatial structure of ACCase changed significantly following the substitution, as indicated by reduced H-bonds. A newly derived cleaved amplified polymorphic sequence (dCAPS) marker was subsequently developed to detect the Trp-2027-Gly mutation in the ACCase of L. chinensis . Additionally, pretreatment with the cytochrome P450 (P450) inhibitor piperonyl butoxide (PBO) and the glutathione S-transferase (GST) inhibitor 4-chloro-7-nitrobenzoxadiazole (NBD-Cl) did not reverse resistance to CyB, suggesting that nontarget-site resistance mechanisms were not involved in CyB resistance in the HFLJ18 population. CONCLUSION Overall, the resistance to CyB in the HFLJ18 population derived from the mutation of ACCase gene, and to the best of our knowledge, this is the first report of the ACCase Trp-2027-Gly mutation conferring resistance to ACCase-inhibiting herbicides in grass species. © 2024 Society of Chemical Industry.
研究领域:
植物科学
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分子生物学
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农业化学
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产品关联
DOI
PubMed
高影响因子
SENSORS
·
2020
·Vol. 20
·期 3
·884
·
IF 3.275
Jinjin Xu,Rongrong Zhang,Chenxi Liu,Aili Sun,Jiong Chen,Zeming Zhang,Xizhi Shi
A highly selective and sensitive molecularly imprinted electrochemiluminescence (MIECL) sensor was developed based on the multiwall carbon nanotube (MWCNT)-enhanced molecularly imprinted quantum dots (MIP-QDs) for the rapid determination of cyfluthrin (CYF). The MIP-QDs fabricated by surface grafting technique exhibited excellent selective recognition to CYF, resulting in a specific decrease of ECL signal at the MWCNT/MIP-QD modified electrode. Under optimal conditions, the MIECL signal was proportional to the logarithm of the CYF concentration in the range of 0.2 µg/L to 1.0 × 103µg/L with a determination coefficient of 0.9983. The detection limit of CYF was 0.05 µg/L, and good recoveries ranging from 86.0% to 98.6% were obtained in practical samples. The proposed MIECL sensor provides a novel, rapid, high sensitivity detection strategy for successfully analyzing CYF in fish and seawater samples.Keywords:molecularly imprinted polymers;quantum dot;cyfluthrin;electrochemiluminescence sensor;fish samples
研究领域:
分析化学
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环境科学
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2022
·
IF 9.231
Wenjuan Wu,Changsheng Li,Desheng Liu,Jiawen Ji,Jianhui Zhu,Huizhe Lu,Bin Fu,Yongqiang Ma
A high-quality antibody production strategy is significant for immunoassay . In this work, four general haptens were proposed based on the 3D structure and surface electrostatic potential of molecular modeling. It was found that the sensitivity and specificity of polyclonal antibodies (pAbs) mainly depended on the bond angle of shapes liked “V” between haptens and proteins and hydrophobic parts of haptens. The quantified process was employed to obtain pAbs against cyhalofop-butyl and its metabolites (CAFs), with the IC 50 value of 4.9 μg·L −1 under optimal conditions. The limit of quantization (LOQ) of the ultrasensitive icELISA in brown rice was 2 μg·kg −1 . The recoveries were 74%–110%, with a coefficient of variation (CV) less than 10%. This study indicated that the hapten property approach led to an improved immunoassay.
研究领域:
免疫学
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分析化学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Xiaoyue Yu,Jinqiu Sun,Yongjie Yang,Jianping Zhang,Yongliang Lu,Wei Tang
This study investigated the multiple herbicide resistance (MHR) mechanism of one Echinochloa crus-galli population that was resistant to florpyrauxifen-benzyl (FPB), cyhalofop-butyl (CHB), and penoxsulam (PEX). This population carried an Ala-122-Asn mutation in the acetolactate synthase (ALS) gene but no mutation in acetyl-CoA carboxylase (ACCase) and transport inhibitor response1 (TIR1) genes. The metabolism rate of PEX was 2-fold higher, and the production of florpyrauxifen-acid and cyhalofop-acid was lower in the resistant population. Malathion and 4-chloro-7-nitrobenzoxadiazole (NBD-Cl) could reverse the resistance, suggesting that cytochrome P450 (CYP450) and glutathione S-transferase (GST) contribute to the enhanced metabolism. According to RNA-seq and qRT-PCR validation, two CYP450 genes (CYP71C42 and CYP71D55), one GST gene (GSTT2), two glycosyltransferase genes (rhamnosyltransferase 1 and IAAGLU), and two ABC transporter genes (ABCG1 and ABCG25) were induced by CHB, FPB, and PEX in the resistant population. This study revealed that the target mutant and enhanced metabolism were involved in the MHR mechanism in E. crus-galli.
研究领域:
植物科学
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杂草科学
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分子生物学
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遗传学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2024
·
IF 4.2
Min Liao,Minghao Jiang,Xumiao Wang,Wei Hu,Ning Zhao,Haiqun Cao
Acetyl-CoA carboxylase (ACCase)-inhibiting herbicides are among the most commonly used herbicides to control grassy weeds, especially Leptochloa chinensis , in rice fields across China. Herein, we collected a suspected resistant (R) population of L. chinensis (HFLJ16) from Lujiang county in Anhui Province. Whole plant dose response tests showed that, compared with the susceptible (S) population, the R population showed high resistance to cyhalofop-butyl (22-fold) and displayed cross-resistance to metamifop (9.7-fold), fenoxaprop- P -ethyl (18.7-fold), quizalofop- P -ethyl (7.6-fold), clodinafop-propargyl (12-fold) and clethodim (8.4-fold). We detected an amino acid substitution (Cys-2088-Arg) in the ACCase of resistant L. chinensis . However, ACCase gene expression levels were not significantly different ( P > 0.05) between R plants and S plants, without or with cyhalofop-butyl treatment. Furthermore, pretreatment with piperonyl butoxide (PBO, a cytochrome P450 monooxygenase (CYP450) inhibitor) or 4-chloro-7-nitrobenzoxadiazole (NBD-Cl, a glutathione-S-transferase (GST) inhibitor), inhibited the resistance of the R population to cyhalofop-butyl significantly (by approximately 60% and 26%, respectively). Liquid chromatography tandem mass spectrometry analysis showed that R plants metabolized cyhalofop-butyl and cyhalofop acid (its metabolite) significantly faster than S plants. Three CYP450 genes, one GST gene, and two ABC transporter genes were induced by cyhalofop-butyl and were overexpressed in the R population. Overall, GST-associated detoxification, CYP450 enhancement, and target-site gene mutation are responsible for the resistance of L. chinensis to cyhalofop-butyl.
研究领域:
植物科学
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分子生物学
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杂草科学
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除草剂抗性
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POLYHEDRON
·
2025
·
IF 2.6
Yuan-Zhen Wang,Liu Yang,Jia-Jia Kou,Yu-Qing Zhan,Yi-Lin Niu,Yu-Long Liu
Flumioxazin, a nitrophenyl herbicide widely used in agriculture, poses potential health risks due to its residue in agricultural products. This study elucidates the fabrication of a novel electrochemical sensor, MnO@C , specifically designed for the efficient detection of flumioxazin. The sensor MnO@C was created by annealing Mn-BTC , synthesized via a straightforward solvothermal method. The electrochemical behavior of MnO@C for detecting flumioxazin was assessed through DPV. The findings revealed that MnO@C demonstrated a wide detection range, achieving an exceptionally low LOD at 597 fmol/L for flumioxazin. Moreover, MnO@C exhibited exceptional duplicability, reliability, and anti-interference ability. The sensor MnO@C was also effectively utilized for the quantitative detection of flumioxazin in diverse samples. The results highlight the substantially practical utility of MnO@C in food and environment detection, which provides broad-spectrum environmental resistance and fast, efficient detection for flumioxazin residue. Additionally, a thorough analysis of the mechanism of MnO@C for monitoring flumioxazin was undertaken.
研究领域:
环境科学
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食品科学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2025
·
IF 11.3
Yingying Qian,Houyu Yu,Qihuan He,Dandan Fan,Gerben J. Zylstra,Hancheng Wang,Xing Huang
The extensive application of cyhalofop-butyl, an aryloxyphenoxypropionate (AOPP) herbicide, for weed control in rice fields inevitably results in environmental pollution and ecological toxicity. Microbial degradation of cyhalofop-butyl is garnering immense research attention. This study revealed that Sphingobium yanoikuyae B1 can efficiently degrade the cyhalofop-butyl, completely breaking down 30 mg/L within 12 h via ester bond hydrolysis, forming cyhalofop acid. A novel hydrolase gene cbeH (37.39% identity) was identified in B1. CbeH exhibited catalytic activity by hydrolyzing the ester bond (C-O) in cyhalofop-butyl and other AOPP herbicides, yielding AOPP acids. Additionally, it catalyzed the cleavage of the C-N bond in amide herbicides propanil and diuron, as well as the C-C bond in the aromatic compound 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid. Sequence alignments and site-directed mutagenesis indicated the catalytic triad Ser-His-Asp in CbeH serves as active center cleaving C-O, C-C, and C-N bonds. Comparative analysis revealed the gene cbeH , highly conserved within Sphingomonads , was located within multiple insertion sequences on the plasmid of Rhodococcus strains. CbeH was widespread in Proteobacteria and Actinobacteria, with its host strains prevalent in aromatic-polluted environments. This study provides novel enzyme resources for remediating combined pollution and enhances our understanding of the genetic organization and environmental distribution of trifunctional pollutant-degrading genes.
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产品关联
DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
·
2019
·
IF 2.883
Xinyun Xu,Jiahao Wang,Ting Yu,Haohan Nian,Hui Zhang,Guangli Wang,Feng Li
Aryloxyphenoxypropionate (AOPP) herbicides with a chiral center are widely used to selectively remove annual and perennial grasses. However, the study of the enzymes involved in enantioselective degradation of AOPP herbicides is limited. A novel family VIII carboxylesterase gene, estwx , hydrolyzed the ester bond cleavage of AOPP herbicides to form the corresponding acid and alkyl side chain alcohol, was cloned from Brevundimonas sp. strain QPT-2 and overexpressed in E. coli BL21. The purified recombinant EstWX was shown to hydrolyze a wide range of AOPP herbicides with various catalytic efficiencies. The enantioselectivity assay indicated that EstWX preferentially catalyzed the hydrolysis of the R -enantiomer of AOPP herbicides. The S73, K76, Y196 together with W368 residues, especially S73 and Y196, were essential for the catalytic function of the carboxylesterase. EstWX is a promising candidate for the bioremediation of multiple AOPP herbicide-contaminated environments and for future mechanism studies on the enantioselective degradation of chiral AOPP herbicides.
研究领域:
环境科学
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微生物学
·
酶学
✓ 已验证
产品关联
DOI
高影响因子
Microbial Cell Factories
·
2017
·Vol. 16
·期 1
·1-10
·
IF 3.681
Zhang Hui,Li Mengya,Li Jie,Wang Guangli,Liu Yuan
Quizalofop- p -ethyl (QPE) is a post-emergence herbicide that effectively controls grass weeds and is often detected in the environment. However, the biochemical and molecular mechanisms of QPE degradation in the environment remains unclear. In this study, a highly effective QPE-degrading bacterial strain J-2 was isolated from acclimated activated sludge and identified as a Pseudomonas sp., containing the QPE breakdown metabolite quizalofop acid (QA) identified by Liquid Chromatography-Ion Trap-Mass Spectrometry (LC-IT-MS n ) analysis. A novel QPE hydrolase esterase-encoding gene qpeH was cloned from strain J-2 and functionally expressed in Escherichia coli BL21 (DE3). The specific activity of recombinant QpeH was 198.9 ± 2.7 U mg −1 for QPE with K
m
and K
cat
values of 41.3 ± 3.6 μM and 127.3 ± 4.5 s −1 . The optimal pH and temperature for the recombinant QpeH were 8.0 and 30 °C, respectively and the enzyme was activated by Ca 2+ , Cd 2+ , Li + , Fe 3+ and Co 2+ and inhibited by Ni 2+ , Fe 2+ , Ag + , DEPC, SDS, Tween 80, Triton X, β-mercaptoethanol, PMSF, and pCMB. In addition, the catalytic efficiency of QpeH toward different AOPP herbicides in descending order was as follows: fenoxaprop-P-ethyl > quizalofop-P-tefuryl > QPE > haloxyfop-P-methyl > cyhalofopbutyl > clodinafop-propargyl. On the basis of the phylogenetic analysis and multiple sequence alignment, the identified enzyme QpeH, was clustered with esterase family V, suggesting a new member of this family because of its low similarity of amino acid sequence with esterases reported previously.
研究领域:
环境科学
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分子生物学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）