SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2024
·
IF 4.3
Liqing Lai,Jinyi Li,Yudong Huang,Huafeng Liu,Xinye Lin,Luqiang Huang,Daliang Li
Mercury, one of the various harmful metals, is particularly significant in affecting aquatic organisms, currently gaining more attentions and sparking discussions. In response to the limitations of traditional detections, fluorescent probes have emerged as a promising solution with some advantages, such as weaker background interference, shorter processing time, higher accuracy. Thus, a novel fluorescent probe, FS-Hg-1 , has been developed for assessing mercury ion (Hg 2+ ) concentrations in aquatic products. This probe displays specific recognition of mercury ions in fluorescence spectra. Notably, FS-Hg-1 exhibits a distinct color change to pink when combined with Hg 2+ (with a 948-fold increase in absorption at 568 nm) and a substantial fluorescence change towards Hg 2+ (361-fold increase, excitation at 562 nm, emission at 594 nm) in N, N-dimethylformamide. The probe boasts a detection limit of 0.14 μM and rapid reaction with Hg 2+ within 10 s, showing an excellent linear correlation with [Hg 2+ ] in the range of 0 to 10 μM. Through thorough analysis using FS-Hg-1 , the results align with those from the standard method ( P > 0.05), with spiked recovery rates ranging from 108.4% to 113.2%. With its precise recognition, low detection limit, and remarkable sensitivity, this fluorescent assay proves effective in mercury concentration determination in aquatic samples without interference. The potential of FS-Hg-1 is promising for speedy detection of residual Hg 2+ and holds significance in ensuring food safety.
研究领域:
环境科学
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分析化学
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食品安全
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产品关联
DOI
PubMed
高影响因子
Foods
·
2021
·Vol. 10
·期 2
·419
·
IF 4.35
Pei Hu,Zhentao Sun,Yunwen Shen,Yiwen Pan
Hydrogen peroxide (H2O2) has been reported to be used for the illegal treatment of fishery products in order to obtain “fake” freshness. Residues of H2O2in food may be of toxicology concern. In this study, a nonenzymatic sensor was developed based on Fe@PCN-224 metal–organic frameworks wrapped by Nafion to detect H2O2concentration. The hybrid structure of Fe@PCN-224 was fabricated by incorporated free FeIIIions into the center of PCN-224, which was ultra-stable due to the strong interactions between Zr6and the carboxyl group. Scanning electron spectroscopy images exhibited that Nafion sheets crossed together on the surface of Fe@PCN-224 nanoparticles to form a hierarchical and coherent structure for efficient electron transfer. Electrochemical investigations showed that the Fe@PCN-224/Nafion/GCE possessed good linearity from 2 to 13,000 μM (including four orders of magnitude), low detection limits (0.7 μM), high stability in continuous monitoring (current remained nearly stable over 2300 s) and in long-term measurement (current decreased 3.4% for 30 days). The prepared nanohybrid modified electrode was effectively applied to H2O2detection in three different fishery products. The results were comparable to those measured using photometrical methods. The developed electrochemical method has a great potential in detecting the illegal management of fishery products with H2O2.Keywords:Fe@PCN-224;Nafion;nonenzymatic sensor;hydrogen peroxide;fishery products
研究领域:
食品安全检测
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电化学分析
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材料科学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 5
·1-9
·
IF 5.3
Zhang Guiyun,Lai Zhiwei,Xie Jianhua,Yang Weiqiang
Methylmercury (CH 3 Hg + ), a lipophilic environmental pollutant, accumulates in fish, shellfish, and other organisms, posing significant risks to human health through the food chain. Developing a convenient and sensitive analytical method for CH 3 Hg + detection is crucial for reducing costs and enhancing the efficiency of food safety testing. In this study, we prepared an octyl-modified silica isoporous membrane on the indium tin oxide (ITO) electrode (Octyl-SIM/ITO) via the electrochemical-assisted self-assembly (EASA) method using octyltrimethoxysilane (O-TES) as the functional organosilane. The Octyl-SIM/ITO electrode exhibits vertically-ordered nanochannels and strong hydrophobic affinity, enabling selective penetration and enrichment of weakly polar analytes. Utilizing square wave anodic stripping voltammetry (SWASV), the Octyl-SIM/ITO electrode demonstrates superior electrochemical response signals for CH 3 Hg + detection, achieving a detection limit as low as 4 nM. This method allows for accurate and reproducible detection of CH 3 Hg + in fish and oyster samples with minimal sample preparation, offering promising potential for portable in situ detection. Graphical
研究领域:
环境科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
Food Analytical Methods
·
2023
·Vol. 16
·期 3
·633-649
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IF 2.9
Li Fan,Wang Zhanhua,Zhu Bingqi,Xu Xiaoying,Liu Zhu
Anesthetics are widely applied in cultivation and transportation of aquatic products to reduce the metabolism of fish and avoid some stress reactions. The overdose of using anesthetics or the occurrence of their residues in the aquatic products may negatively affect human health after consumption. A rapid screening procedure and a new analytical method for the simultaneous determination of 14 anesthetics was established in this study. The compounds were extracted from fish and shrimp matrices by sodium acetate buffer solution, cleaned up via solid-phase extraction, and then were analyzed using an ultrahigh-performance liquid chromatography system coupled with a tandem quadrupole-time-of-flight mass spectrometer (UPLC-Q-TOF–MS) in a positive-ion mode. Methodological verification analyses, including linearity, precision, recovery, limit of detection (LOD), and limit of quantification (LOQ), were conducted to validate the method. The correlation coefficient of the calibration curves of each anesthetic exceeded 0.99. The average recoveries of this analysis method varied from 72.36 to 111.27% with relative standard deviations less than 5%. The detection limit of anesthetics reached 0.3–0.7 μg/kg. Agilent Mass Hunter Personal Compound Database Library (PCDL) Manager was used to establish a database of 14 anesthetics and 8 isotope internal standards, which enabled a quick screening and analysis without standard substances. The results of the spiked blank samples demonstrated reliability of the screening method with mass deviations less than 5 ppm and the scores determined by relative molecular mass, retention time, isotope ratio, and distribution all greater than 85. In addition, according to the fragmentation information of mass spectrometry, the fragmentation rules of some anesthetics were summarized. This method was validated with reliable results in quantitative and qualitative analysis and could be used as regulatory measures for anesthetics in aquatic products.
研究领域:
食品安全
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分析化学
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环境科学
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产品关联
DOI
高影响因子
Antibiotics-Basel
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2022
·Vol. 11
·期 10
·1427
·
IF 5.222
Hongli Ye,Siman Li,Yinfeng Xi,Yongfu Shi,Xiaorui Shang,Dongmei Huang
Antibiotic drug residues are crucial to ensure food safety and minimize risk to human health. Herein, a sensitive high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS) method was developed and validated for the determination of antibiotic residues (mainly amphenicols) consisting of chloramphenicol (CAP), thiamphenicol (TAP), florfenicol (FF), and florfenicol amine (FFA) in aquatic products. Amphenicols were well separated on a Kinetex F5 (100 mm × 3.0 mm, 2.6 µm) chromatographic column with the mobile phases of 1 mM ammonium acetate aqueous solution and methanol solution and measured after positive and negative electrospray ionizations using four internal standards. To our knowledge, it was the first time to report the good performance of F5 column and four internal standards for the determination of amphenicols. The established method featured a good linear relationship between chromatographic peak area ratios and the concentrations of amphenicols (R2> 0.992), a wide and low detection matrix-based range of 0.01–5 μg/L, a low detection limit of 0.01 μg/kg, etc. The spiked assays evidenced the accuracy and reliability of the developed method with the recoveries between 84.0 and 105%, the intraday relative standard deviations (RSDs) over the range of 0.769–13.7%, and the interday RSDs over the range of 0.582–13.3%. Finally, the proposed method was applied to investigate amphenicol residues in various aquatic products, including fish, shrimp, crab, shellfish, and other aquatic species.Keywords:antibiotics;amphenicols;detection;sensitivity;aquatic products;HPLC–MS/MS
研究领域:
食品安全
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分析化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2021
·Vol. 26
·期 16
·4975
·
IF 4.412
Xuan Zhang,Yunhua Hui,Changling Fang,Yuan Wang,Feng Han,Xiaoyi Lou,Essy Kouadio Fodjo,Youqiong Cai,Cong Kong
A sensitive and reliable method was developed to determine methylene blue (MB) and its metabolite residues, including azure A (AZA), azure B (AZB), and azure C (AZC) in aquatic products by HPLC–MS/MS. The samples were extracted by acetonitrile and cleaned up by alumina-neutral (ALN) cartridges. The analytes were separated on a Sunfire C18 column (150 mm × 2.1 mm, 5 µm). The method was validated according to the European criteria of Commission Decision 2002/657/CE. Good linearity between 1–500 µg/L was obtained with correlation coefficients (R2) greater than 0.99. The limit of quantification (LOQ) was 1.0 µg/kg. The average recoveries at three levels of each compound (1, 5, and 10 µg/kg) were demonstrated to be in the range of 71.8–97.5%, with relative standard deviations (RSDs) from 1.05% to 8.63%. This method was suitable for the detection of methylene blue and its metabolite residues in aquatic products.Keywords:high-performance liquid chromatography–tandem mass spectrometry;methylene blue;disinfectant;aquatic products
研究领域:
分析化学
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食品安全
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Xuan Zhang,Changling Fang,Dongmei Huang,Guangxin Yang,Yunyu Tang,Yongfu Shi,Cong Kong,Pei Cao,Youqiong Cai
A method for the determination of 8 biogenic amines in aquatic products and their derived products was established by HPLC-MS/MS without derivatization. The samples were extracted by 5% perchloric acid solution. N -hexane was used to clean the extract. The analytes were separated by a column of ACQUITY UPLC HSS T3 (100 mm × 2.1 mm, 1.8 µm), and gradient eluted with a mixed solution of (0.5% formic acid) and acetonitrile. Good linearity was obtained with correlation coefficients (R 2 ) >0.99. This method achieved higher sensitivity (from 0.1 mg/kg for tyramine, 2-phenylethylamine and tryptamine to 1.0 mg/kg for spermidine, spermine, cadaverin, histamine and putrescine). The average recoveries were demonstrated in the range of 70.9%–113.1%, with relative standard deviations (RSDs) from 0.33% to 10.81%. This method was suitable for the detection of BAs in aquatic products and their products.
研究领域:
食品科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2016
·
IF 4.052
Zheng-zhong Lin,Hong-yuan Zhang,Ai-hong Peng,Yi-dong Lin,Lu Li,Zhi-yong Huang
Magnetic molecularly imprinted polymers (MMIPs) were synthesized through precipitation polymerization using malachite green (MG) as template, methacrylic acid as monomer, ethylene dimethacrylate as crosslinker, and Fe 3 O 4 magnetite as magnetic component. MMIPs were characterized by scanning electron microscopy, Fourier transform infrared spectrometry, and vibrating sample magnetometry. Under the optimum condition, the MMIPs obtained exhibited quick binding kinetics and high affinity to MG in the solution. Scatchard plot analysis revealed that the MMIPs contained only one type of binding site with dissociation constant of 24.0 μg mL −1 . The selectivity experiment confirmed that the MMIPs exhibited higher selective binding capacity for MG than its structurally related compound (e.g., crystal violet). As a sorbent for the extraction of MG in sample preparation, MMIPs together with the absorbed analytes could easily be separated from the sample matrix with an external magnet. After elution with methanol/acetic acid (9:1, v/v), MG in the eluent was determined by high-performance liquid chromatography coupled with UV detector with recoveries of 94.0–115%. Results indicated that the as-prepared MMIPs are promising materials for MG analysis in aquatic products.
研究领域:
分析化学
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食品安全
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE CHINESE CHEMICAL SOCIETY
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2021
·Vol. 68
·期 9
·1739-1747
·
IF 1.967
Xiaoqing Wang,Jing Zhang,Zimeng Xu,Chen Rao,Leilei Pi,Yaqin Fu,Yubing Dong,Chen Shen,Lanying Yao,Chunhua Xiong
The MCTS-g-AT was developed as a novel magnetic adsorbent with core-shell structure. Its unique synthesis route endows MCTS-g-AT with green, convenient, economical and safe characteristics, and also good selective adsorption of Hg(II). The material was characterized using an appropriate set of spectroscopic and analytical techniques (SEM, EDS, XRD, VSM, FTIR, and TGA). Its adsorption kinetics fits the pseudo-secondary model well and adsorption isotherm fits the Langmuir equation well, suggesting the mechanism of chemisorption with monolayer adsorption. The results of the thermodynamic parameters indicate spontaneous and endothermic process. Column adsorption experiments showed that the maximum adsorption capacity of Hg(II) was 207.6 mg/g (298 K). Moreover, the Hg(II) adsorbed on composite microspheres was completely eluted by 3 mol/L HNO 3 or 4 mol/L HCl. Furthermore, MCTS-g-AT adsorption column combined with spectrophotometer can achieve more accurate detection of Hg(II) in aquatic products.
研究领域:
环境科学
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材料科学
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分析化学
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DOI
高影响因子
Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment
·
2020
·
IF 2.34
Leilei Chen,Haipu Li,Yang Liu,Yue Li,Zhaoguang Yang
The occurrence of 28 common antibiotics in wild and farmed aquatic products from different species was comprehensively investigated in the present study. The results showed that a larger number of antibiotics could be detected quantitatively in wild samples, while the farmed samples had higher concentrations. Twenty, 17, and 14 target compounds were found in the muscles of wild molluscs, wild fish and farmed fish with total concentrations of 2.02–16.4 ng g–1, 0.51–11.9 ng g–1, and
研究领域:
环境科学
·
公共卫生
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毒理学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2013
·Vol. 29
·期 4
·653-656
·
IF 0.735
Wang Wen-wen,Gao Fu-kai,Li Gui-zhi,Liu Zhen-bo,Liu Yong-ming
A simple, environmentally friendly and high efficient extraction method was proposed for the determination of five phthalates in aquatic products by gas chromatography combined with mass spectrometry detector (GC-MS). When this method was adopted, samples were pretreated by modified QuEChERS(quick, easy, cheap, effective, rugged and safe) method. An environmentally friendly extractant(ethanol aqueous solution) replaced toxic acetonitrile for extracting phthalates in the protein-matrix samples. Fluorescence quenching spectra of bovine serum albumin(BSA) with phthalates show that there was a high-affinity interaction between phthalate and protein, decreasing the extraction efficiency of phthalates from fish samples. However, in the 80%(volume fraction, φ ) ethanol aqueous solution, a slow but full protein denaturation takes place, which would cause the unfolding of protein and the release of phthalates. Meanwhile, the fat-soluble impurities are extracted less in φ (ethanol) 80% aqueous solution than in either φ (ethanol) 100% or hydrophobic solvents in the liquid-liquid extraction procedure. Therefore the purification steps were greatly simplified. Overall recoveries were 81.77%–90.5% with limits of detection between 2.53 and 9.61 μg/L, and relative standard deviation values at 1.15%–4.85%. The proposed approach was satisfactorily applied to the determination of phthalates in real aquatic products, such as fish, shrimp and oyster.
研究领域:
分析化学
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环境科学
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产品关联
DOI
高影响因子
Chemosensors
·
2022
·Vol. 10
·期 11
·457
·
IF 4.229
Zhenyu Lu,Yufei Hu,Gongke Li,Ling Xia
A fluorescence adamantane three-dimensional porous organic framework (AdaPOF) was synthesized via a Suzuki coupling reaction. The AdaPOF showed excellent fluorescence performance with a relative high quantum yield and fluorescence stability. Due to its excellent selectivity to tetracycline (TC), a fluorescence sensor based on AdaPOF was constructed for TC determination. The selective sensing mechanism of the AdaPOF towards TC was studied by density functional theory (DFT) calculation experiments. An AdaPOF–based fluorescence method for TC determination was established, with the linear range of 0.1–9.0 μmol/L (R2= 0.9959) and the limit of detection (S/N= 3) of 43 nmol/L. Moreover, the fluorescence method was used to the determination of TC in aquatic products and the recoveries were ranged from 94.4% to 103.8%. The results obtained by this fluorescence method were consistent with those of the high-performance liquid chromatography (HPLC) method in the TC determination.Keywords:adamantane;porous organic framework;tetracycline;fluorescence sensor;aquatic products
研究领域:
分析化学
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食品科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）