Journal of Water Process Engineering
·
2022
·
IF 7.34
Yonglei Wang,Xinglin Wang,Liping Tian,Yanan Li,Guilin He,Haiyang Yu,Hua Qi,Wentao Sun
Algal blooms in lake and reservoir water lead to water quality problems, including water odors, algal toxins, and disinfection byproducts . A novel dissolved air flotation process that incorporates positively charged bubbles (PosiDAF) can effectively remove algae in lakes and reservoirs. Chitosan, as a modified bubble agent in the PosiDAF process, has attracted extensive attention due to its natural nontoxicity and good flocculation properties. In this paper, three types of amphiphilic chitosan with different lengths of carbon chains were prepared via amphiphilic modification and were used as bubble modifiers to remove microcystis aeruginosa in the PosiDAF process. The effects of algae removal under different dosage of modifiers were compared, and the role of algal organic matter was investigated. Results showed that the water solubility of chitosan was markedly enhanced after amphiphilic modification, while thermal stability were decreased. When using butyl-N-2 hydroxypropyl trimethyl chitosan ammonium chloride (C4-HTCC), the effect of modified air flotation was the best, and the removal ratio of algae was 93.47% when the C4-HTCC dosage was 1.0 mg/L. The presence of AOM enhanced the overall effect of algae removal, and electrostatic attraction was the primary adhesion mechanism of the microbubble-amphiphilic chitosan-algal organic matter-algal cell system. In addition, adsorption bridging and net trapping also have positive effects on algae removal.
研究领域:
环境科学
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水处理技术
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DOI
高影响因子
Environmental Science and Ecotechnology
·
2024
·
IF 14
Yinjie Zhu,Jian Ding,Xiaoxiong Wang,Xuejian Wang,Huansheng Cao,Fei Teng,Shishi Yao,Zhiru Lin,Yuelu Jiang,Yi Tao
Harmful cyanobacterial blooms (HCBs) pose a global ecological threat. Ultraviolet C (UVC) irradiation at 254 nm is a promising method for controlling cyanobacterial proliferation, but the growth suppression is temporary. Resuscitation remains a challenge with UVC application, necessitating alternative strategies for lethal effects. Here, we show synergistic inhibition of Microcystis aeruginosa using ultraviolet A (UVA) pre-irradiation before UVC. We find that low-dosage UVA pre-irradiation (1.5 J cm −2 ) combined with UVC (0.085 J cm −2 ) reduces 85% more cell densities compared to UVC alone (0.085 J cm −2 ) and triggers mazEF -mediated regulated cell death (RCD), which led to cell lysis, while high-dosage UVA pre-irradiations (7.5 and 14.7 J cm −2 ) increase cell densities by 75–155%. Our oxygen evolution tests and transcriptomic analysis indicate that UVA pre-irradiation damages photosystem I (PSI) and, when combined with UVC-induced PSII damage, synergistically inhibits photosynthesis. However, higher UVA dosages activate the SOS response, facilitating the repair of UVC-induced DNA damage. This study highlights the impact of UVA pre-irradiation on UVC suppression of cyanobacteria and proposes a practical strategy for improved HCBs control.
研究领域:
环境科学
·
微生物学
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Wei Xia,Yuyang Wang,Shaohang Shen,Hu Yang
Harmful algal blooms (HABs) are becoming increasingly frequent in freshwater lakes and coastal water. In this study, a novel actinia-shaped composite coagulant (ATP@CS) comprising cationic starch (CS) and attapulgite (ATP), covalently bonded by a silane coupling agent, was successfully designed and fabricated for HAB mitigation. ATP@CS showed superior performance in the harvesting of Microcystis aeruginosa , a kind of typical algae, with a maximum harvesting efficiency exceeding 98.0%. The embedded ATP in ATP@CS as the weighted inert remarkably improved the settlement properties of the formed flocs. Settling velocity increased with increased ATP content in this composite coagulant, and complete settlement was immediately achieved after coagulation by ATP@CS with an ATP-to-CS mass ratio higher than 2.0. A higher coagulant dosage was required to achieve the optimum alga removal efficiency due to the charge neutralization and bridging effects of the CS as the tentacle of this actinia-shaped composite coagulant. The presence of ATP in this composite coagulant promoted the formation of large, compact, durable, and rapidly regrown flocs during coagulation. ATP@CS as an efficient coagulant has notable potential applications in HAB removal from water owing to its remarkable advantages of enhanced settlement property, high ecological safe, notable HAB removal efficiency, low cost, and environment friendliness.
研究领域:
环境科学
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水处理技术
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产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Wei Xia,Dapeng Jiang,Jintao Liu,Jun Cai,Zhonghua Xi,Hu Yang
A series of novel cationic modified actinia-shaped composite coagulant (AMS-C), with similar tentacle length and distribution but different charge density (CD), was successfully designed and fabricated by combination of a cationic graft starch and attapulgite (ATP). AMS-C shows a high efficiency in coagulative removal of Microcystis aeruginosa from water over a wide pH range. The algae-harvesting efficiency of optimized AMS-C can reach to 92.27% only within 1.0 min after settlement and its maximal harvesting efficiency is as high as 99.25% at the optimum dosage of 1.5 mg/L. This can be attributed to its special composited structure with abundant cationic long tentacle chains. CD of AMS-C is a key structural factor. AMS-C with a relatively high CD obviously enhanced the coagulation efficiency and settling performance through the improved charge neutralization, besides, the distinct long tentacle chains of AMS-C allowed its easy accessibility and tightly contacted with the algal cells, and thus facilitated the formation of large, dense and fast regrowing algal flocs by the enhanced bridging and sweeping effects. The aforementioned effects were together contributed to the effective removal of algae. The effective interactions between microalga cells and the composite coagulants were also verified using extended Deryaguin-Landau-Verwey-Overbeek theory. Moreover, AMS-C was able to remove Microcystins-LR without destroying the cells, and still maintained a high algae-harvesting efficiency in real water bodies. Therefore, AMS-C, with the advantages of high-performance, environmentally-friendliness and low-cost, has notably promising application prospects in effective treatment of harmful algal blooms.
研究领域:
环境科学
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生物学
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DOI
PubMed
高影响因子
WATER RESEARCH
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2017
·
IF 6.942
Zong-Yao Qian,Jian Ma,Chun-lei Sun,Zhi-Gang Li,Qi-Ming Xian,Ting-Ting Gong,Bin Xu
Freshwater resources are under stress around the world due to rapid urbanization and excessive water consumption. Cyanobacterial blooms have occurred frequently in surface waters, which produced toxic secondary metabolites causing a potential harm to aquatic ecosystems and humans. In this study, the relationship between different types of nitrogen source and the algal growth of Anabaena flos-aquae , which was isolated from Dianchi Lake in southern China, was investigated. Experiments were accomplished by using four types of isotope tracers including 15 N-ammonium chloride, 15 N-sodium nitrate, 15 N-urea, 15 N- l -alanine in culture medium to characterize the biosynthesis of 15 N-anatoxin-a (ATX-A), which is a major algal toxin from A. flos-aquae , through liquid chromatography-tandem mass spectrometry (LC-MS/MS). The results showed that all these four types of nitrogen can be incorporated into algal cells. The ATX-A production with urea as the nitrogen source was much higher than that with the other three types of nitrogen. The 15 N labeling experiments further demonstrated that the uptake of organic nitrogen nutrients was significantly greater than that of inorganic nitrogen . These results provide new evidence and deeper insight to explore the biosynthesis of ATX-A in the specific strain of A. flos-aquae .
研究领域:
环境科学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2019
·
IF 4.527
Peng Gu,Qi Li,Weizhen Zhang,Zheng Zheng,Xingzhang Luo
Eastern China is a typical region that suffers from harmful cyanobacterial blooms. Numerous studies have focused on bloom formation mechanisms; however, the detailed mechanisms remained unclear. Our study explored the influence of four metal ions (Ca, Cu, Pb and Cd) on Microcystis aeruginosa to determine their effects on bloom formation. We found that Ca concentrations higher than 100 mg L −1 contributed to cyanobacterial bloom formation. The presence of Ca triggered the anti-oxidation process and promoted the secretion of extracellular polysaccharides , thus inducing aggregation of algal cells and enhancing their buoyancy 2.1-fold more than the control ( p ＜0.05). The reverse regulation of dissolved CO 2 to bicarbonate by carbonic anhydrase formed a large amount of carbonate and decreased the growth rate by 38–56%. Cu (>0.1 mg L −1 ) presented significant toxicity to algal cells while Pb (>1 mg L −1 ) suppressed the algal growth rate due to the acidic condition. Cd (<0.1 mg L −1 ) exhibited no apparent toxicity to the algae. Furthermore, as the buoyancy increased, Cd was likely to facilitate the formation of cyanobacterial blooms, which needs further research. These findings can provide a theoretical basis for eutrophic lake management and contribute to the development of water quality and wastewater discharge standards.
研究领域:
环境科学
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水生生态学
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水资源管理
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产品关联
DOI
PubMed
高影响因子
Water
·
2025
·Vol. 17
·期 17
·2561
·
IF 3
Xiaojia Huang,Yuanyuan Hu,Xiaowei Cheng,Weibin Pan
Cylindrospermopsis raciborskiiis a toxin-producing cyanobacterium that is easy to overlook. It has strong environmental adaptability and is currently spreading around the world and gradually dominating to form a persistent bloom, causing ecological and environmental risks and drinking water safety issues. In this study, we systematically investigated the inhibitory effects of oleic acid onC. raciborskiiand elucidated the underlying mechanisms through morphological observation, physiological assays, and bioinformatics analysis. Our results demonstrated that oleic acid strongly inhibits the growth ofC. raciborskii, with a 72 h half-maximal effective concentration (EC50) of 0.903 mg·L−1. At 1.6 mg·L−1, oleic acid achieved an inhibition rate of 99.5% within 48 h, indicating rapid suppression of cyanobacterial growth. Physiological analyses revealed that oleic acid severely impaired photosynthetic activity, as evidenced by significant reductions in key parameters (rETRmax, α, Fv/Fm, and Fv/Fo) and altered photosynthetic pigment composition, suggesting structural and functional damage to the photosynthetic apparatus. Morphological observations further showed that oleic acid disrupted filament integrity, inducing cell shrinkage, cytoplasmic vacuolation, cell wall detachment, membrane rupture, and eventual cellular disintegration. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis indicated that oleic acid interferes with multiple metabolic processes, including nutrient and cofactor synthesis, membrane transport, and signal transduction, ultimately triggering algal cell death. This study highlights oleic acid as a promising eco-friendly agent for mitigatingC. raciborskiiblooms, offering potential applications in ecological prevention and emergency bloom control.
研究领域:
环境微生物学
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水生生态学
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毒理学
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分子生理学
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藻类生物学
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生态毒理学
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产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2025
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IF 11.3
Yu Wu,Linna Shao,Cai Cheng,Xianyong Kou,Shi Huang,Liexiong Zhang,Jie Yu,Xuehan Xu,Xingyu Xiang,Xiaoliang Guo,Lingling Feng
Harmful Microcystis blooms (HMBs) and their hepatotoxic metabolites microcystins (MCs) pose escalating threats to aquatic biodiversity and public health due to their recalcitrance to conventional remediation approaches. To address this dual challenge, mesoporous silica (MSN) and hollow mesoporous organosilica (HMON) nanostructures were engineered via structure-guided design principles, enabling the development of the crude extract microcystinase A (CMlrA)-tailored immobilized platform (CMlrA@MSN and CMlrA@HMON) for synchronized HMBs suppression and MCs detoxification. Systematic optimization of carrier physicochemical properties (including specific surface area, hydrophilicity, and pore size) significantly enhanced the immobilization efficiency and catalytic durability of CMlrA. Kinetic modeling revealed 201.3% and 44.1% K cat / K m enhancements in MC-LR degradation for CMlrA@MSN and CMlrA@HMON relative to free CMlrA. Immobilized enzyme systems achieved 77.06% and 83.03% growth inhibition rates in Microcystis aeruginosa FACHB-905 after six-day treatment. This biocatalytic immobilization strategy establishes an eco-efficient framework for synergistic HMB suppression and MC degradation, providing the technical groundwork for practical water remediation systems.
研究领域:
环境科学
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生物技术
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生态毒理学
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DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2017
·Vol. 25
·期 7
·6300-6307
·
IF 2.741
Zhang Li,Liu Jutao,Zhang Dawen,Luo Linguang,Liao Qiegen,Yuan Lijuan,Wu Naicheng
Poyang Lake is the largest freshwater lake in China and an important drinking water source. Since the year 2000, toxic cyanobacteria have been observed frequently in Poyang Lake. In the present study, spatial and seasonal variations of microcystins (MCs; MC-RR, MC-YR, and MC-LR) in water column were examined monthly from January to December (except the months of March, May, and November) in 2013, by using ultra-high-performance liquid chromatography-electrospray ionization tandem triple quadrupole/mass spectrometry (UPLC-MS/MS). MC-RR was the most dominant variant, followed by MC-LR, while MC-YR was detected in low concentration. Total MC concentrations (intracellular + extracellular MCs) ranged from 1.26 to 9916.05 ng/L, with an average of 469.99 ng/L, and only 3.14% (6 out of 192 samples) of the water samples contained MC concentrations that exceeded the drinking water guideline level of 1 μg/L for MC-LR proposed by the World Health Organization (WHO). MC concentrations in water column showed obvious seasonal variations in Poyang Lake. Intracellular and extracellular MCs were both at a low level from January to April but increased quickly from June to August and decreased dramatically thereafter. Intracellular MCs exhibited similar spatial distribution pattern with extracellular MCs. Both intracellular and extracellular MC concentrations in eastern bays and around Songmen Mountain of Poyang Lake were higher than other regions. Intracellular MC concentrations were positively correlated with Chl a ( r = 046, P < 0.01), pH ( r = 0.25, P < 0.01), cyanobacterial biomass ( r = 0.40, P < 0.01), and temperature ( r = 0.36, P < 0.01) but negatively correlated with TN ( r = − 0.28, P < 0.01), suggesting that TN, cyanobacteria biomass, pH, and temperature might be regulating factors for MC production in Poyang Lake.
研究领域:
环境科学
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湖泊生态学
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水生毒理学
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环境监测
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淡水生态学
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DOI
高影响因子
WATER RESEARCH
·
2016
·
IF 5.991
Haomin Huang,Xi Xiao,Fang Lin,Hans-Peter Grossart,Zeyu Nie,Lijuan Sun,Chen Xu,Jiyan Shi
The effects of allelochemicals on cyanobacterial blooms have been observed for more than 20 years; however, the use of these compounds, usually involving a “direct-added” mode, has clear disadvantages, such as a short activity period or temporarily excessive localized concentration. Here, a simulated-allelopathy mode to facilitate the application of allelochemicals was proposed and tested on Microcystis aeruginosa . The continuous-release beads of 5,4′-dihydroxyflavone (DHF) were constitutive of a polymer matrix and showed a high drug-loading rate (47.18%) and encapsulation efficiency (67.65%) with a theoretical release time of approximately 120 d. Cyanobacterial growth tests showed that the DHF beads had long-term inhibition effects (>30 d), whereas those of “direct-added” DHF to cells lasted a maximum of 10 d. The beads also continuously affected the superoxide dismutase, catalase , and lipid peroxidation of M. aeruginosa . The inhibitory effects of DHF beads on cyanobacterial growth increased as initial cell densities of M. aeruginosa decreased, suggesting that the beads inhibit cyanobacterial activity more effectively in the early bloom phase. Consequently, the anti-cyanobacterial beads represent a novel application mode of allelochemicals with long-term inhibitory effects on cyanobacterial growth. Our study demonstrates that the successful application of allelochemicals offers great potential to control harmful cyanobacterial blooms, especially at the initial stage of development.
研究领域:
环境科学
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水生生态学
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植物学
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产品关联
DOI
PubMed
高影响因子
Journal of Environmental Sciences
·
2025
·
IF 6.3
Wei Zhang,Xu Zeng,Jiayi Dong,Yibin Su,Erik Jeppesen,Liqing Wang,Sicheng Yin
Microorganism-based biocontrol strategies for harmful cyanobacterial blooms have gained increasing attention due to their ecological compatibility and species-specific targeting. Despite this, research on algicidal bacteria effectiveness against the invasive cyanobacterium Raphidiopsis raciborskii remains limited. Research has been conducted on the Bacillus subtilis strain CH21, which was isolated from Chaohu Lake in China. exhibiting potent indirect anticyanobacterial activity against R. raciborskii . Within 48 h of exposure, the cell-free filtrate of strain CH21 achieved 96.2 % removal efficiency of R. raciborskii cells. Physiological and morphological analyses revealed severe disruption of the photosynthetic apparatus (Fv/Fm and Yield values reduced to zero within 16 h), oxidative stress (elevation in malondialdehyde content and reactive oxygen species, reduction in superoxide dismutase and catalase activity), and cellular damage, including pseudovacuole collapse, the degradation of polyphosphate granules and cytoplasmic leakage. Metabolomic profiling identified surfactin-class lipopeptides as putative bioactive agents, which demonstrated exceptional stability across a wide thermal (25–121 °C) and pH (3–13) range. These findings highlight B. subtilis CH21 as a promising candidate for targeted biocontrol of R. raciborskii blooms, supporting the development of environmentally mitigation technologies to be used in cases where it is difficult to control R. raciborskii by external nutrient loading reduction or if the response to such loading reductions are slow.
研究领域:
微生物学
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环境科学
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应用生态学
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生物技术
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藻类学
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代谢组学
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产品关联
DOI
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2023
·Vol. 30
·期 15
·45388-45397
·
IF 5.8
Li Min,Wang Yayao,Xiao Jianxing,Yan Xingfu,Liu Bingru
Allelochemicals are essential agents for the biological control of harmful blooms. It is crucial to identify efficient algal suppressors and understand their mechanisms. This study reports the inhibition of Microcystis aeruginosa growth by 6 phenolic acids derived from plants’ secondary metabolites. The inhibitory effect of phenolic acids was significantly influenced by exposure dose and phenolic acid species. Caffeic acid has the most efficient algal inhibition ability (96 h-EC 50 of 5.8 mg/L). In contrast, the other 5 analogs (cinnamic acid, p -coumaric acid, 3-hydroxycinnamic acid, ferulic acid, and isoferulic acid) showed a weak inhibition effect or promotion effect with the exposure dose of 5–100 mg/L. ROS and chlorophyll a content tests combined with metabolomics analysis revealed that caffeic acid could induce the ROS accumulation of M. aeruginosa . They mainly disturbed nucleotide, amino acid, and fatty acid metabolism, leading to the downregulation of most metabolites, including toxins of microcystin LR and cyanopeptolin A, and the precursors of some unpleasant terpenoids. It has been suggested that caffeic acid is an effective agent for controlling M. aeruginosa blooms.
研究领域:
环境科学
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水生生态学
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植物化学
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毒理学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）