Tissue Engineering Part C-Methods
·
2024
·Vol. 30
·期 3
·113-129
·
IF 2.7
Xiaoxiao Li,Weikang Zhao,Dandan Zhou,Pei Li,Chen Zhao,Qiang Zhou,Yiyang Wang
The decellularized extracellular matrix (ECM) of cartilage is a widely used natural bioscaffold for constructing tissue-engineered cartilage due to its good biocompatibility and regeneration properties. However, current decellularization methods for accessing decellularized cartilaginous tissues require multiple steps and a relatively long duration to produce decellularized cartilage. In addition, most decellularization strategies lead to damage of the microstructure and loss of functional components of the cartilaginous matrix. In this study, a novel decellularization strategy based on a hydrostatic pressure (HP) bioreactor was introduced, which aimed to improve the efficiency of producing integral decellularized cartilage pieces by combining physical and chemical decellularization methods in a perfusing manner. Two types of cartilaginous tissues, auricular cartilage (AC) and nucleus pulposus (NP) fibrocartilage, were selected for comparison of the effects of ordinary, positive, and negative HP-based decellularization according to the cell clearance ratio, microstructural changes, ECM components, and mechanical properties. The results indicated that applying positive HP improved the efficiency of producing decellularized AC, but no significant differences in decellularization efficiency were found between the ordinary and negative HP-treated groups. However, compared with the ordinary HP treatment, the application of the positive or negative HP did not affect the efficiency of decellularized NP productions. Moreover, neither positive nor negative HP influenced the preservation of the microstructure and components of the AC matrix. However, applying negative HP disarranged the fibril distribution of the NP matrix and reduced glycosaminoglycans and collagen type II contents, two essential ECM components. In addition, the positive HP was beneficial for maintaining the mechanical properties of decellularized cartilage. The recellularization experiments also verified the good biocompatibility of the decellularized cartilage produced by the present bioreactor-based decellularization method under positive HP. Overall, applying positive HP-based decellularization resulted in a superior effect on the production of close-to-natural scaffolds for cartilage tissue engineering.Impact statementIn this study, we successfully constructed a novel hydrostatic pressure (HP) bioreactor and used this equipment to produce decellularized cartilage by combining physical and chemical decellularization methods in a perfusing manner. We found that positive HP-based decellularization could improve the production efficiency of integral decellularized cartilage pieces and promote the maintenance of matrix components and mechanical properties. This new decellularization strategy exhibited a superior effect in the production of close-to-natural scaffolds and positively impacts cartilage tissue engineering.
研究领域:
组织工程
·
生物医学工程
·
软骨生物学
·
机械工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2022
·Vol. 11
·期 15
·2190
·
IF 5.561
Yaqiong Zhang,Zhi-Hong Zhang,Ronghai He,Riyi Xu,Lei Zhang,Xianli Gao
Using high hydrostatic pressure (HHP) to treat liquid foods can improve their aroma; however, no information about the effects of HHP on soy sauce aroma has yet been reported. The effects of HHP on the aroma of soy sauce fermented for 30 d were investigated using quantitative descriptive analysis (QDA), SPME–GC–olfactometry/MS, hierarchical cluster analysis (HCA) and principal component analysis (PCA). Results showed that the pressure used during HHP treatment had a greater influence on soy sauce aroma than the duration of HHP. Compared to the control, soy sauce that was treated with HHP at 400 MPa for 30 min (HHP400–30) obtained the highest sensory score (33% higher) by increasing its sour (7%), malty (9%), floral (27%) and caramel-like (47%) aromas, while decreasing its alcoholic (6%), fruity (6%) and smoky (12%) aromas; moreover, the aroma of HHP400–30 soy sauce was comparable with that of soy sauce fermented for 180 d. Further investigation demonstrated that HHP (400 MPa/30 min) enhanced the OAVs of compounds with sour (19%), malty (37%), floral (37%), caramel-like (49%) and other aromas (118%), and lowered the OAVs of compounds with alcoholic (5%), fruity (12%) and smoky (17%) aromas. These results were consistent with the results of the QDA. HHP treatment positively regulated the Maillard, oxidation and hydrolysis reactions in raw soy sauce, which resulted in the improvement and accelerated formation of raw soy sauce aroma. HHP was capable of simultaneously improving raw soy sauce aroma while accelerating its aroma formation, and this could treatment become a new alternative process involved in the production of high-quality soy sauce.Keywords:soy sauce;high hydrostatic pressure;aroma compound;Aspergillus oryzae;soybean
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Nanoelectronics and Optoelectronics
·
2017
·Vol. 12
·期 10
·1056-1061
·
IF 0.497
Li Shengbin,Hu Bingxu,Zhang Fan
In this paper, we report the fabrication and characterizations of polyvinylidene fluoride (PVDF)/chitosan and PVDF/polymeric methyl methacrylate (PMMA)/titanium oxide (TiO2) materials membranes for waterproofing applications. The detailed results revealed that with the increase
of chitosan proportion, the anti-hydrostatic pressure value of PVDF/chitosan raised first and then declined. When the proportion of PVDF and chitosan reaches 92:8, the anti-hydrostatic pressure value reaches to 5437 Pa, however, when chitosan exceeds the value, its hydrophobicity was reduced.
In addition, the fibrous membrane prepared from PVDF/PMMA/TiO2 was adopted to make a thermal treatment and 6% TiO2 nanoparticles were added to improve the intensity and anti-water pressure of the fibers. The detailed experiments revealed that when the reaction temperature
reach to 50 °C, the anti-hydrostatic pressure of fibrous membrane is far better than fibrous membrane prepared from PVDF/chitosan. Thus, the results confirmed that by adding 6% TiO2 nanoparticles improves the anti-hydrostatic pressure of fibrous membrane. The observed results
shows that chitosan and TiO2 blended with PVDF are promising material for waterproofing applications.
研究领域:
材料科学
·
高分子化学
✓ 已验证
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2018
·
IF 3.52
Qiang Xia,Yunfei Li
This investigation aimed to examine the effects of a novel processing pattern, combining high hydrostatic pressure (HHP) with germination, on the embryo growth and physicochemical characteristics of wholegrain brown rice (WBR). WBR grains were firstly subjected to mild HHP stress (30–90 MPa/5 min) and then incubated at 37 °C for 52 h for obtaining germinated samples (GBR). The results showed that HHP shock resulted in a delayed embryo growth of WBR grains, maintaining acceptable sprouting rates ranging from 65% to 76% when germination was finished. The contents of gama-aminobutyric acid in GBR were greatly increased responding to HHP stress, showing pressure intensities dependent. Total digestible and resistant starch contents in samples stressed at 60/90 MPa were decreased, mainly associated with high pressure-induced amorphization as revealed by SEM imaging and FTIR , which promoted starch hydrolysis during germination. Besides, the levels of zinc and iron were influenced by HHP pretreatments due to the high pressure-mediated degradation behavior for phytic acids. The storability of HHP-stressed GBR grains was significantly enhanced through reducing free fatty acids formation and maintaining color stability during a storage testing. These results obtained from the current work demonstrated that mild HHP stress pretreatment prior to germination process could be used as a promising strategy to modulate certain physicochemical characteristics of WBR products.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Zhongzheng Xu,Mingwei Zhao,Yiming Zhang,Pan Wang,Yining Wu,Lin Li,Xin Cui,Ning Sun,Caili Dai
Superhydrophobic wettability inspired by the “lotus effect” is an intriguing property in nature, but it is rarely applied in oilfield development. Superhydrophobic nanomaterials can effectively solve the problem of “high injection pressure and insufficient water injection” in low permeability oilfields by changing the properties of the core surface, which is of great significance to the development of low permeability oilfields. Herein, we report a novel type of superhydrophobic nanoparticle (SHNP) simply modified by fluorinated long chains, and a stable nanofluid is successfully prepared by compounding surfactant. The prepared SHNP nanofluid has an excellent core drag reduction effect, and the drag reduction rate is 1.35 times that of conventional NPs under the same conditions. The SHNPs assemble on the core surface to form a large number of micro/nanorough structures, which can effectively reduce the surface roughness of the core. The PIV experimental results show that compared with the injection of pure water, the center flow velocity in the subsequent water flooding center increases by 98.27% after the injection of SHNPs nanofluid (the flow velocity decreases by 53.45% after the injection of NPs). Moreover, bubble probe AFM technology has successfully shown that bubbles can be captured through the SHNPs superhydrophobic interface to form a gaseous film. By using the barrier effect of the gaseous film, the liquid–solid interface is converted to a liquid–gas-solid interface, thereby reducing the large resistance caused by the direct contact between the liquid and solid. Such SHNPs with simple modification and low cost have broad application potential in oilfield development.
研究领域:
石油工程
·
材料科学
·
界面化学
✓ 已验证
产品关联
DOI
高影响因子
CHEMOSPHERE
·
2020
·
IF 5.778
Melani Purnika Mudugamuwa Arachchige,Taihua Mu,Mengmei Ma
Novel pectin derived from sweet potato residue was modified by high hydrostatic pressure (HHP)-assisted pectinase and then used for Pb 2+ removal from aqueous solutions. The removal characteristics and mechanisms were also investigated. Results showed that modified sweet potato pectin exhibited greater adsorption performances for Pb 2+ than that of natural ones, and showed excellent eco-friendly properties and good potential for adsorption of some other heavy metals (such as Cu 2+ ). The adsorption curves were much more conformed to Langmuir model, and the highest capacity for Pb 2+ adsorption was 263.15 mg/g with 1.00% pectin at pH 7. Chemical adsorption process of pectin for Pb 2+ absorption involved O-containing functional groups (O–H, COO-), cation exchange, and along with electrostatic interactions. Overall, the results in this study indicated that sweet potato pectin modified with HHP-assisted pectinase had the potential to become an environmentally friendly coagulant-flocculant agent for the heavy metal adsorption, especially for Pb 2+ .
研究领域:
食品科学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2020
·
IF 5.778
Junliang Wang,Weicheng Zheng,Yuqing Zhang,Shuang Song,I-Ming Chou,Mian Hu,Zhiyan Pan
Degradation of phenol by sodium persulfate (SPS) in hot compressed water (HCW) was investigated in a lab-built fused quartz tube reactor (FQTR) coupled with Raman spectroscopy system. The species of S 2 O 8 2− , SO 4 2− , HSO 4 − , SO 3 2− and HSO 3 − in the reaction system were qualitatively and quantitatively analyzed by Raman spectroscopy. The hydrothermal stability of phenol and SPS at different temperature and the degradation of phenol by SPS were also studied. The results indicated that phenol was not stable in aqueous solution above 200 °C, and that only SO 4 2− was generated in the hydrolysis of SPS at temperatures below 50 °C, and SO 4 2− and HSO 4 − were generated at higher temperatures. The maximum conversion rate (90.93%) and mineralization efficiency (38.88%) of phenol by SPS was obtained at reaction temperature of 300 °C with 180 min reaction time. During the degradation of phenol by SPS, HSO 4 − was the main product and S∗ (not detected by Raman spectroscopy) exhibits a positive correlation with temperature. In addition, a degradation pathway of phenol by SPS was proposed. The degradation data for the kinetic analysis indicated that the reaction followed pseudo first-order kinetics, and the reaction rate constants ( k s ) were given as k 50 °C = 0.0083 min −1 , k 100 °C = 0.0197 min −1 , k 200 °C = 0.0498 min −1 , k 300 °C = 0.0619 min −1 and k 400 °C = 0.0505 min −1 at 30 min reaction. Moreover, the activation energy (12.580 kJ mol −1 ), the enthalpy change (9.064 kJ mol −1 ) and the entropy change (−222.104 J mol −1 ) of the reaction were also calculated.
研究领域:
环境科学
·
化学工程
·
光谱学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Chemistry Frontiers
·
2019
·Vol. 3
·期 6
·1052-1061
·
IF 0
Tingting Lin,Xing Su,Kai Wang,Minjie Li,Hongwei Guo,Lulu Liu,Bo Zou,Yu-Mo Zhang,Yifei Liu,Sean Xiao-An Zhang
Stimuli-responsive fluorescent materials which can change their optical signal outputs upon changes in ambient physical or chemical parameters exhibit great application potential for comprehensive environmental monitoring. In this article, a new aggregation-induced emission fluorescent switch, 1',3′,3′-trimethyl-5′-(4-(1,2,2-triphenylvinyl)phenyl)spiro[chromene-2,2′-indoline]-6-carbonitrile (TPE-Sp-CN), was synthesized and applied in the quantitative detection of three parameters, namely pH value, sulfite anion and hydrostatic pressure, with different molecular states. According to the analysis of the optical spectra, TPE-Sp-CN can distinguish water pH values from 0 to 4, the detection limit of sulfite anion is 1.04 ppm, and the pressure sensing range is 1 atm to 6 GPa with the sensing sensitivity of a 9.876 nm GPa−1 shift in the PL spectrum. Furthermore, TPE-Sp-CN can quantitatively sense all three parameters with good linear relationships, which is rarely reported. Also, it is promising to use TPE-Sp-CN as an indicator to monitor the environment or disasters that are related to the above parameters, such as harmful geological activities.
研究领域:
材料科学
·
环境监测
·
化学传感
✓ 已验证
产品关联
DOI
FOOD CHEMISTRY
·
2022
·
IF 9.231
Sinan Zhang,Zhenhong Zheng,Chuyao Zheng,Yadong Zhao,Zhuo Jiang
The mechanism of the high hydrostatic pressure (HHP) effect on horseradish peroxidase (HRP) is still unclear. The activity, thermal stability and structural changes of HRP after HHP treatments were studied in this work. Compared with the untreated sample, the enzyme activity reduces by 36% after 800 MPa processing. The results indicated that the conformation of the enzyme active center changes under pressure. Furthermore, HHP also changes the conformation of disulfide bonds and some secondary structures in HRP. These structural and conformational changes induce decreased activity. In addition, differential thermal scanning (DSC) results showed that the thermal denaturation temperature decreased from 103.74 °C to 85.78 °C after pressure treatment, suggesting HRP molecules formed large aggregates after pressure treatment. In this study, the interaction mechanism between pressure and enzyme was studied as well, and the results can provide some guidance for the application of HHP technology in fruit and vegetable products processing.
研究领域:
食品科学
·
酶学
✓ 已验证
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Mingwei Zhao,Yuxin Xie,Lin Li,Caili Dai,Zhongzheng Xu,Yunbo Dong,Hongbo Zeng
Decreasing injection pressure and increasing water injection are crucial methods to enhance oil recovery in low permeability reservoirs. However, the high injection pressure, constrained by the reservoir's low porosity and permeability, limits the amount of injected water, consequently resulting in low recovery. In this study, inspired by the ''lotus effect'' of superhydrophobic wettability, we synthesized superhydrophobic nanoparticles by grafting fluorine-containing long chains onto the surfaces of SiO 2 nanoparticles. Nanofluids with small particle size (7 nm), high stability, and strong hydrophobicity (162°) were prepared by compounding modified SiO 2 nanoparticles with surfactants. The functional properties of the nanofluids revealed a depressurization rate of 23.13 % and the 23.90 % increase in flow rate at the channel center. Modified nanoparticles altered the surface wettability from hydrophilic to superhydrophobic. Surface free energy, determined using a three-probe-liquid method, demonstrated a significant reduction after hydrophobic modification. The surface hydrophobic interaction was tested and quantified by AFM measurements. The results show that the adhesion force is much larger than that of the unmodified hydrophilic surface. Through these analyses, we propose an efficient depressurization mechanism facilitated by the superhydrophobic layer formed by modified SiO 2 nanoparticles. This work offers technical and theoretical support for the efficient development of low permeability reservoirs.
研究领域:
石油工程
·
材料科学
·
界面化学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Xiangxiang Sun,Ahmed S.M. Saleh,Yifan Lu,Zhuangzhuang Sun,Xiuyun Zhang,Xiangzhen Ge,Huishan Shen,Xiuzhu Yu,Wenhao Li
In this study, proso millet starch was isolated and subjected to treatment with ultra-high pressure (UHP), cold plasma (CP), or their combination to modify its functional properties. The changes in structural, physicochemical, and digestive properties of proso millet starch after these treatments were investigated. The proso millet native starch granules showed irregular and polygonal shapes with a smooth surface. Treatments with CP or UHP at low pressures did not change the morphological properties or crystalline structure type of proso millet starch granules, while the treatment with UHP at 600 MPa and CP resulted in a complete gelatinization of starch. Also, UHP treatment at high pressure, followed by CP treatment, destroyed the partial crystalline region and reduced the short-range order of proso millet starch. Besides, a combination of UHP and CP treatment promoted the depolymerization of long chains in proso millet starch. Moreover, the combined treatments could enhance the resistance to high temperature and shearing and improve the pasting stability of starch. Furthermore, the combined treatment could increase the slowly digestible starch content. Therefore, the combination of UHP and CP treatment can be suggested for modifying the functional properties of proso millet starch and promoting its industrial applications.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2024
·
IF 5.3
Lin Li,Yunbo Dong,Xiao Jin,Zizhao Wang,Jinming Liu,Caili Dai
In water injection development of low-permeability reservoirs, the elevated flow resistance between the rock surface and the injected water results in enhanced injection pressure. Reducing the thickness of interfacial water film to expand flow radius is a new thought to achieve a decrease in injection pressure. In this work, a hydroxyl-containing cationic surfactant tetradecyl methyl dihydroxy-ethyl ammonium bromide (HTTAB) was designed and synthesized. The introduction of hydroxyl groups enhances the binding to the negatively-charged rock surface. HTTAB can modify the strong hydrophilic rock surface to a hydrophobic state, with the water contact angle increasing from 20.2° to 110.3°. HTTAB shows excellent drag-reducing effect, maintaining a drag reduction rate of over 30 % even after 30 PV of flushing. Particle Image Velocimetry (PIV) experiments show that after HTTAB adsorption, the interfacial slip velocity and centerline velocity can increase by 1.67 times and 33.66 % respectively, and the flow radius expands significantly. In addition, the thickness of the interfacial water film is obtained using Atomic Force Microscope (AFM). The results show that the thickness of the interfacial water film is reduced significantly from 79.42 nm to 8.85 nm. This study provides important guidance for the design of drag-reducing agents and the understanding of interfacial drag-reduction behavior.
研究领域:
石油工程
·
界面化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）