FOOD RESEARCH INTERNATIONAL
·
2024
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IF 7
Chenyu Song,Wei Xu,Cuie Guang,Ting Xue,Wanmeng Mu
Patulin (PAT) is a mycotoxin commonly found in fruits and vegetables, prompting the need for effective removal and detoxification methods, which have garnered significant research attention in recent years. Among these methods, the utilization of microbial-derived enzymes stands out due to their mild operating conditions, specificity in targeted functional groups, and the production of non-toxic by-products, making it a preferred degradation approach. In this study, a novel PAT-degrading enzyme derived from Cyberlindnera fabianii ( Cyfa -SDR) was identified, demonstrating its highest catalytic activity at pH 7.0 and 80 °C against PAT. This temperature tolerance level represents the highest reported for PAT-degrading enzymes to date. The enzyme was further characterized as a short-chain dehydrogenase through analysis of its amino acid composition, conserved GXXXGXG motif, and dependency on NADPH. Moreover, the study evaluated the efficiency of PAT degradation by Cyfa -SDR at varying substrate and enzyme concentrations, surpassing the performance of other PAT-degrading enzymes, thus highlighting its substantial potential for the biological control of PAT. In conclusion, the enzymatic treatment using the PAT-degrading enzyme Cyfa -SDR presents a viable and promising solution for enhancing the quality and safety of fruit juice.
研究领域:
食品科学
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微生物学
·
酶学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2021
·Vol. 13
·期 15
·1795-1802
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IF 2.896
Feng Wang,De-Bin Wan,Yu-Dong Shen,Yuan-Xin Tian,Zhi-Li Xiao,Zhen-Lin Xu,Jin-Yi Yang,Yuan-Ming Sun,Bruce D. Hammock,Hong Wang
The natural mycotoxin tenuazonic acid (TeA) in foods is identified as the most toxic mycotoxin among the over 70 kinds of secondary toxic metabolites produced by Alternaria alternata. Some hapten–antibody-mediated immunoassays have been developed for TeA detection in food samples, but these methods show unsatisfactory sensitivity and specificity. In this study, a rationally designed hapten for TeA mycotoxin generated with computer-assisted modeling was prepared to produce a highly specific camel polyclonal antibody, and an indirect competitive chemiluminescence enzyme immunoassay (icCLEIA) was established with a limit of detection of 0.2 ng mL−1 under optimized conditions. The cross-reactivity results showed that several analogs and some common mycotoxins had negligible recognition by the anti-TeA polyclonal antibody. The average recoveries spiked in fruit juices were determined to be 92.7% with an acceptable coefficient of variation, and good correlations between icCLEIA and liquid chromatography tandem mass spectrometry (LC-MS/MS) results were obtained in spiked samples. This developed icCLEIA for TeA detection with significantly improved sensitivity and satisfactory specificity is a promising alternative for environmental monitoring and food safety.
研究领域:
食品科学
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免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2025
·
IF 6.1
Wenxuan Li,Yaxi Xu,Meifei Wang,Yuyang Wang,Jinchao Wei,Di Chen
A simple and efficient effervescence-assisted salting-out assisted liquid-liquid extraction (EA-SALLE) method was developed for the rapid extraction of pyrethroid insecticides in fruit juices and herbal extracts. By integrating effervescence extraction with SALLE, the extraction and phase separation processes were conducted simultaneously, significantly simplifying the experimental procedure and reducing the extraction time to just 3 min. Key factors influencing the method's efficiency were systematically optimized. Combined with gas chromatography with an electron capture detector, enabled the determination of nine pyrethroid insecticides, demonstrating excellent linearity (R 2 > 0.99), low limits of detection (0.03–0.17 ng/mL), satisfactory accuracy (recoveries ranging from 83.0 % to 107.9 %), and good precision (RSD < 9.1 %). The proposed EA-SALLE method offers a simple, efficient, and cost-effective sample preparation technique. It is characterized by ease of operation, short extraction time, and high sensitivity, providing an effective tool for the monitoring and assessment of pesticide residues in complex samples.
研究领域:
分析化学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2019
·
IF 3.858
Yinghe Ji,Zhaorui Meng,Jing Zhao,Hanqing Zhao,Longshan Zhao
A sensitive, fast, eco-friendly ultrasound assisted liquid–liquid microextraction (LLME) in combination with hydrophobic deep eutectic solvent (DES) method was developed to preconcentrate and extract sulfonamides in fruit juices, prior to high-performance liquid chromatographic (HPLC) analysis. The DES was synthesized with trioctylmethylammonium chloride (TAC) and 2-octanol at the molar ratio of 1:2. Some parameters which affected the extraction efficiency were investigated and optimized, including the volume of DES, extraction method, extraction time, pH. Under the optimum conditions, good linearity ( r = 0.9999) in the range of 0.1–50 µg mL −1 was obtained. The limit of detection (LOD) was 0.02–0.05 µg mL −1 . And the accuracy of the developed method was confirmed by analysis of spike method, the recoveries were in the range of 88.09–97.84% at the spike levels of 2–20 µg mL −1 in fruit juices.
研究领域:
分析化学
·
环境科学
·
食品科学
✓ 已验证
DOI
高影响因子
ANALYTICAL LETTERS
·
2024
·
IF 1.6
Xinxing Jiang,Jihong Fu,Dandan Li,Wenxia Xue,Yaxue Zhang
An effective and sensitive SERS substrate (TPDH-COF/Au NPs) on a covalent organic framework (TPDH-COF) decorated with gold nanoparticles (Au NPs) was fabricated via electrostatic self-assembly and successfully applied for the determination of thiabendazole (TBZ) in fruit juice. The “raspberry-like” structure SERS substrate combined the properties of the abundant “hot spots” between the Au NPs and the adsorption performance of TPDH-COFs for trace analytes. Excellent SERS performance was observed, which achieved a high enhancement factor value of 2.01 × 108 for rhodamine 6 G, with considerable reproducibility and excellent long-term stability of at least 28 d. The limit of detection was estimated to be 5 ppb for TBZ in fruit juice. The result demonstrated that the TPDH-COF/Au NPs have great potential for SERS analyses and determination of pesticide residues in foods.
研究领域:
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Xianqi Wang,Jie Wang,Beibei Hu,Yuyue Zhang,Hongzhe Tian
Nitrogen doped carbon quantum dots (N-CQDs) were synthesized by a hydrothermal method. A fluorescence sensor for rapid determination of organophosphorus pesticides residue was developed using the N-CQDs based on acetylcholine enzyme inhibition. Cu 2+ effectively quenched the strong fluorescence of N-CQDs, which could be recovered due to the complexation between copper ions and thiocholine, the hydrolysis product of acetylthiocholine catalyzed by acetylcholinesterase. Organophosphorus pesticides inhibited the activity of acetylcholinesterase, resulting in fluorescence quenching of N-CQDs. Fluorescence inhibiting efficiency depends on the concentration of the analytes, and therefore, the sensor could be used for quantitative analysis of organophosphorus pesticides residue. The average recoveries of the analytes ranged from 85.33 % to 110.67 % at the spiked concentrations of 0.015 mg/L, 0.04 mg/L and 0.1 mg/L, and the limits of detection were 2.89 ∼6.40 ng/mL. The developed method had the advantages of simple operation, fast speed, low cost, expensive apparatus-free, and environmental friendliness, which could be used for rapid screening of organophosphorus pesticides residue in real samples.
研究领域:
分析化学
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环境科学
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食品科学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Yongna Zhao,Dandan Du,Qiannan Li,Wenjie Chen,Qingchen Li,Qingzhu Zhang,Ning Liang
A highly binding dummy template molecularly imprinted polymers (Fe 3 O 4 -NH 2 @GO@MIPs) was synthesized used Fe 3 O 4 -NH 2 @GO as carrier by dummy surface molecular imprinting technique (DSMIT) for the first time. Fe 3 O 4 -NH 2 @GO@MIPs were characterized by fourier transform infrared (FT-IR), scanning electron microscope (SEM), transmission electron microscope (TEM), thermogravimetric analysis (TGA), brunauer emmett teller (BET) and vibrating specimen magnetometer (VSM). Adsorption and selectivity experiments of Fe 3 O 4 -NH 2 @GO@MIPs and non-imprinted polymers (Fe 3 O 4 -NH 2 @GO@NIPs) verified that the Fe 3 O 4 -NH 2 @GO@MIPs had a better selectivity and adsorption properties for five pyrethroids pesticides. Adsorption test results revealed that the adsorption behaviors of pyrethroids onto Fe 3 O 4 -NH 2 @GO@MIPs followed Langmuir equation adsorption isotherm and pseudo-second-order kinetic models. Fe 3 O 4 -NH 2 @GO@MIPs were used as sorbent in magnetic solid phase extraction (MSPE) to extract five pyrethroids from fruit juice samples, following desorption with acidified ethyl acetate, the pyrethroids were quantified by gas chromatography with electron capture detection (GC-ECD). The developed method MSPE coupled with GC-ECD achieved satisfactory recovery (72.1–106.8%) with RSD less than 10.8%, and the detection limits (LOD) for fenpropathrin (FENP), lambda-cyhalothrin (LC), cyfluthrin (CYF), fenvalerate (FEN) and deltamethrin (DEL) ranged from 0.007 to 0.015 μg/L. Compared to other methods, the procedure MSPE-GC-ECD based on Fe 3 O 4 -NH 2 @GO@MIPs in this work demonstrated to be a more convenient and practical method for the separation, enrichment, and detection of pyrethroids pesticides from fruit juice samples.
研究领域:
分析化学
·
环境科学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）