FUEL
·
2024
·
IF 6.7
Fengli Yang,Qing Xia,Guangpin Lin,Yi Su,Wei Lin,Huabing Zhang,Guobin Liang
Saccharide is the main product of biomass hydrolysis, which can be converted into high-value-added chemicals and fuels, and the isomerization of glucose to fructose is a decisive step in biomass conversion technology. In this research, the isomerization reaction of glucose catalyzed by MgCl 2 -700 in water has been studied, and the fructose yield and selectivity reached 36.4 % and 79.3 %, respectively in an aqueous system at 120 °C for 60 min. The results of electrospray ionization tandem mass spectrometry (ESI-MS) and density functional theory (DFT) show that [MgCl(aq)] + formed in an aqueous system coordinated with glucose for isomerization reaction, and the binding of C1/C2 sites of the β-glucopyranose and [MgCl(aq)] + promoted the 1,2-intramolecular hydride shift of glucose, thus rapidly converting glucose into fructose.
研究领域:
生物化学
·
催化化学
✓ 已验证
DOI
高影响因子
THERMOCHIMICA ACTA
·
2011
·
IF 1.908
Xiaohong Zhao,Xueqiao Xie,Yongsheng Yan
Binodal data of poly(propylene glycol) PPG 400 + salt ((NH 4 ) 2 SO 4 , MgSO 4 , and KCl) + H 2 O systems were determined at T = 298.15 K, and binodal data of PPG 400 + KAc + H 2 O system was determined at T = (298.15, 308.15, and 318.15) K. Moreover, the binodal curves were fitted to Merchuk equation, and the tie-lines were fitted to both the Othmer–Tobias and Bancroft equations. The effects of temperature and salts on the binodals and tie-lines of the investigated ATPSs were also studied. Finally, the salting-out ability of salts on the polymer was obtained by fitting the Setschenow-type equation to the tie-line data to derive a salting-out coefficient ( k S ) for each salt. For potassium salts, the relative effectiveness of the anions in inducing the phase segregation in ATPSs follows the Hofmeister series. The relative salting-out effectiveness of the sulfate salts is influenced by complexation of the cations by PPG.
研究领域:
化学工程
·
生物技术
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DOI
高影响因子
LAB ON A CHIP
·
2021
·Vol. 21
·期 14
·2684-2690
·
IF 6.799
Chunmei Zhou,Pingan Zhu,Xing Han,Rui Shi,Ye Tian,Liqiu Wang
An aqueous two-phase system (ATPS) is considered as a promising candidate for biological applications due to its excellent permeability, selective separation, rapid mass transfer and all-biocompatible nature. However, it remains difficult to generate ATPS emulsions with controllable and prolonged stability due to ultra-low interfacial tension. Here, we present a transient double emulsion (TDE) technique to address this challenge. The method involves two steps: (i) water-in-oil-in-water (W1/O/W2) transient double emulsion droplets are first produced with droplet microfluidics by introducing an additional middle oil phase, and (ii) the W1/O/W2 droplets dewet into oil-in-water and ATPS droplets in a controllable manner. Using the TDE method, both dextran-in-polyethylene glycol (PEG) and PEG-in-dextran ATPS droplets can be generated with high uniformity (coefficient of variation (CV) ranging from 0.66% to 2.55%), a wide range of droplet sizes (∼100 to 250 μm in radius), and tunability in the generation frequency (∼4 to 170 Hz). Tuning the oil viscosity controls the destabilization time for on-demand dewetting and releasing of aqueous core droplets. The stability of ATPS droplets is significantly improved by storing aqueous droplets in double emulsion vessels, which would benefit various applications, such as small molecule encapsulation.
研究领域:
生物工程
·
化学工程
·
材料科学
✓ 已验证
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2020
·
IF 5.065
Yajing Pan,Xiao Sun,Man Qi,Ru Qin,Xiangjing Che,Yongqiang Zhang
The growing demand for vanadium and molybdenum in the high-tech industry has spurred researches into recycling from a variety of sources. A green and simple process for separation of vanadium and molybdenum was proposed by aqueous two-phase systems consisting of non-ionic surfactants (triblock copolymer, L35; octaphenyl polyoxyethylene, Triton X-100 and polyethylene glycol , PEG2000) and sodium sulfate without any extractant. The effects of aqueous pH, temperature, vanadium and molybdenum concentration，and different surfactants on separation performance of vanadium and molybdenum were systematically studied. The results indicated that the extraction rate of molybdenum and vanadium increased from 73% to 92% and from 11% to 30%, respectively, with increasing aqueous pH from 0.6 to 0.9. The extraction rate of vanadium decreased and the extraction rate of molybdenum increased with increasing temperature from 313.15 K to 343.15 K. Furthermore, with strengthening of molybdenum concentration from 1 g/L to 10 g/L and vanadium concentration from 1 g/L to 5 g/L, the extraction rate of vanadium and molybdenum increased. The sequence on separation efficiency of both metals is L35, Triton X-100 and PEG2000 from high to low. The extraction rate of molybdenum reached 91% and the extraction rate of vanadium was below 18% and the separation factor of both metals was above 47 under the optimal conditions of aqueous pH of 0.70–0.75 and extracting temperature of 343.15 K for L35 + Na 2 SO 4 + H 2 O aqueous two-phase system. It has obvious advantages in terms of safety production, environmental protection compared with traditional organic solvent extraction method .
研究领域:
化学工程
·
环境科学
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产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2013
·
IF 2.487
Qiang Wu,Dong-Qiang Lin,Shan-Jing Yao
The aqueous two-phase system (ATPS) with polyethylene glycol (PEG) and hydroxypropyl starch (HPS) was evaluated for the extraction of human immunoglobulin G (IgG) from human serum albumin (HSA). The partitions of IgG in PEG/sulphate, PEG/phosphate, PEG/Dextran and PEG/HPS ATPSs were compared, and the results indicated that PEG/HPS system was most suitable for IgG extraction. The effects of the concentrations of PEG, HPS and NaCl addition and pH on the partition of IgG and HSA in PEG/HPS ATPS were investigated. It was found that with 15% NaCl addition at pH 8.0 IgG could be largely recovered in the top PEG-rich phase and most of HSA kept in the bottom phase. In addition, the response surface methodology (RSM) was used to optimize the extraction of IgG from the protein mixture of IgG and HSA. The optimal conditions were obtained as 12% (w/w) PEG 4000, 18% (w/w) HPS and 10% (w/w) NaCl at pH 8.0. The extraction yield of IgG in the top phase was 99.2% and the purification factor could reach 5.28. The back extractions of IgG into a phosphate-rich bottom phase were also studied. The total purification factor was 5.73 with the yield of 84.0%. The results indicated that PEG/HPS ATPS might be a promising alternative for the primary recovery of IgG from the complicated feedstock.
研究领域:
生物化学
·
蛋白质工程
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DOI
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2021
·Vol. 193
·期 11
·3753-3764
·
IF 2.926
Chen Xiaochun,Huang Xiaoquan,Tang Yiwen,Zhang Lei
Nuclease P1 (NP1) can hydrolyze nucleic acids into four 5′-mononucleotides, which are widely used in the pharmaceutical and food industries. In this paper, an aqueous two-phase system (ATPS) was developed to purify NP1 from Penicillium citrinum . Polyethylene glycol (PEG) and nucleotides salts were studied to form ATPSs, among which PEG3000/disodium guanosine monophosphate (GMPNa 2 ) was researched, including the phase composition and pH. Using 14% (w/w) PEG3000 and 20% (w/w) GMPNa 2 ATPS at pH 5.0, the best recovery and purification factor, 82.4% and 3.59, were obtained. The recovery of NP1 was 98.3% by the separation of ultrafiltration from the PEG-rich phase. The recycling use of GMPNa 2 was also studied, and 95.1% of GMPNa 2 in the salt-rich phase was obtained with the addition of ethanol as the solvent. These results showed that the ATPS was effective for purification of NP1.
研究领域:
生物化学
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工业微生物学
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产品关联
DOI
PubMed
高影响因子
BIOTECHNOLOGY LETTERS
·
2025
·Vol. 47
·期 1
·1-11
·
IF 2.1
Fu Xiao-Qian,Leong Hui-Yi,Qiao Liang-Zhi,Zhou Jia-Nan,Hu Wei,Yao Shan-Jing,Lin Dong-Qiang
Objective Adeno-associated viruses (AAVs) are widely used as gene therapy vectors due to their safety, stability, and long-term expression characteristics. The objective of this work is to develop an aqueous two-phase system (ATPS) as a universal platform for the separation and purification of AAVs. Results This study utilized polyethylene glycol (PEG)/salt ATPSs to separate and purify various AAV serotypes, including AAV5, AAV8, and AAV9, which focusing on serotype-specific performance and partial empty capsid removal. The results showed that all the AAV serotypes were mainly enriched in the interphase of ATPS, with achieving high recovery (> 95%) and impurity removal (> 95%). The PEG/sodium citrate ATPS was serotype-independent, but the process optimization of component concentrations for each serotype was necessary to attain the best performance. Notably, a single-step aqueous two-phase extraction also demonstrated the ability to remove some amount of empty capsids from the crude cell lysate, with removal rate ranging from 4 to 25%. Conclusions The results demonstrated the practical applicability of PEG/sodium citrate ATPS in separating and purifying different AAV serotypes, which addressing key challenges in gene therapy vector production.
研究领域:
生物技术
·
病毒学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LANGMUIR
·
2019
·
IF 3.683
Jie Li,Feng Zhang,Leilei Jiang,Liang Yu,Lixiong Zhang
In the present work, a novel microchannel device was developed and used for the preparation of core–shell microspheres combining with a dextran/poly(ethylene glycol) diacrylate (DEX/PEGDA) aqueous two-phase system. Silica@silica core–shell microspheres were prepared as a model material. Silica@silica core–shell microspheres with different sizes of cores and thicknesses of shells were prepared by using different flowrate ratios of DEX/silica and PEGDA/silica aqueous solutions. The content of colloidal silica and the calcination temperature have a significant effect on the texture properties of the prepared core–shell microspheres. The surface area decreased from 199 to 177 m2/g with an increase in the colloidal silica content from 30 to 60 wt %. For a specific colloidal silica content (50 wt %), with the increase in calcination temperature from room temperature to 650 °C, the total pore volume went through a maximum of 0.7 cm3 g–1 with a surface area of 178 m2 g–1 and pore size of 7.32 nm at 450 °C. Due to the accumulation of metal nanoparticles in DEX, different metal nanoparticles (Ni and Pd) were successfully introduced into the core of the core–shell microspheres for the preparation of silica/metal nanoparticles@silica core–shell microsphere catalysts. The catalysts showed similar catalytic performance as the metal nanoparticles for hydrogenation of 4-nitrophenol with a conversion higher than 95%. However, the core–shell microsphere catalyst is much easier to recover. The reuse experiments indicated that the core–shell catalyst has high stability.
研究领域:
材料科学
·
化学工程
·
催化化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
·
2017
·
IF 2.497
Hongxia Pan,Ting Yang,Jialing Chen,Xuejun Cao
Cefprozil, a semi-synthetic antibiotic, has been used in pediatric therapy. The purpose of this study was to improve the yield of cefprozil and obtain purer product in an aqueous two-phase system composed of P ADB and P MDB . P ADB and P MDB were synthesized using acrylic acid, methacrylic acid, 2-dimethylaminoethyl methacrylate, and butyl methacrylate. Cefprozil was synthesized with 7-amino-3-(1-propenyl)-4-cephalosporanic acid (7-APRA) as the main ring, ( R )-amino-(4-hydroxyphenyl) acetic acid methyl ester hydrochloride (D-HPGME-HCl) as the acyl donor and the immobilized penicillin acylase as the catalyst. 7-APRA and D-HPGME-HCl were concentrated in the P MDB -rich phase, while cefprozil was in the P ADB -rich phase. The optimal partitiona coefficient of cefprozil was 1.84 with 40 mM KSCN, while the enzyme reaction yield of cefprozil reached 99.39% compared with 77.61% in the aqueous-phase system. The recovery of the two copolymers was up to 96.01% at pH 3.54. After crystallization, the purity of the product reached 88.02%, and no copolymer remained in the product. The combination of cefprozil∙DMF was detected by gas chromatography-mass spectrometry, FT-IR spectroscopy, thermogravimetric analysis, and electrospray ionization mass spectrometry. The results showed that no chemical bond existed between cefprozil and DMF.
研究领域:
药物化学
·
生物工程
·
制药工程
✓ 已验证
产品关联
DOI
高影响因子
CHINESE JOURNAL OF CATALYSIS
·
2021
·
IF 8.271
Shujing Zhang,Hong Ma,Yuxia Sun,Xin Liu,Meiyun Zhang,Yang Luo,Jin Gao,Jie Xu
Tandem catalysis for the hydrogenation rearrangement of furfural (FA) provides an attractive solution for manufacturing cyclopentanone (CPO) from renewable biomass resources. The CuNi/Al-MCM-41 catalyst was synthesized and afforded excellent catalytic performance with 99.0% conversion and 97.7% selectivity to CPO in a near-neutral solution under 2.0 MPa H 2 at 160 °C for 5 h, much higher than those on other molecular sieve supports including MCM-41, SBA-15, HY, and ZSM-5. A small amount of Al highly dispersed in MCM-41 plays an anchoring role and ensures the formation of highly dispersed CuNi bimetallic nanoparticles (NPs). The remarkably improved catalytic performance may be attributed to the bimetallic synergistic and charge transfer effects. In addition, the initial FA concentration and the aqueous system pH required precise control to minimize polymerization and achieve high selectivity of CPO. Fourier transform infrared spectroscopy and mass spectra results indicated that polymerization was sensitive to pH values. Under acidic conditions, FA and intermediate furfuryl alcohol polymerize, while the intermediate 4-hydroxy-2-cyclopentenone mainly polymerizes under alkaline conditions, blocking the cascade of multiple reactions. Therefore, near-neutral conditions are most suitable for minimizing the impact of polymerization. This study provides a useful solution for the current universal problems of polymerization side reactions and low carbon balance for biomass conversion .
研究领域:
催化化学
·
生物质转化
·
材料科学
✓ 已验证
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2017
·
IF 3.359
Ai He,Bing Dong,Xueting Feng,Shun Yao
In this research, two types of aqueous two-phase systems (ATPS) based on n -alkyl-tropinium and n -alkyl-quinolinium bromide ionic liquids (ILs) + salt + water have been developed and studied experimentally at different temperature. The binodal curves are fitted to nonlinear Merchuk relationship. For both investigated ILs, the longer the cation alkyl chain is, the greater its ability for ATPSs formation is; meanwhile the ability of the potential salts used for phase separation was compared and sorted, and high temperature was not beneficial to form these tropinium/quinolinium ILs–based ATPSs. On the basis of above results, the new method of extraction for ginsenosides from crude extracts of Panax Ginseng C. A. Mey using IL-ATPS was developed for the first time. Under appropriate conditions, a relatively high extraction efficiency (99.5%), partition coefficient (K = 651) and selective enrichment for total saponins could be obtained. Furthermore, the target ginsenosides can be recovered from the IL-rich phase and the enrichment is conducive to improve their antioxidant activity. Above research is expected to provide meaningful reference for the separation of similar bioactive compounds.
研究领域:
植物化学
·
分离科学
·
天然产物研究
✓ 已验证
DOI
高影响因子
ACS Sustainable Chemistry & Engineering
·
2025
·
IF 7.3
Xiang Chen,Hao Li,Yichao Lu,Zhichun Liu,Zhongsen Ma,Yajie Zhang
2,5-Furandicarboxylic acid (FDCA) has been extensively verified as a biobased aromatic ring platform compound. The development of mild and efficient novel oxidation technologies is important for FDCA preparation technologies. In this study, the efficient oxidation of 5-hydroxymethylfurfural (HMF) to produce FDCA by the TEMPO/NaClO/KBr single-phase aqueous catalytic oxidation system was systematically investigated, and the mechanism was analyzed in detail. Under the optimal reaction conditions, TEMPO as the main catalyst directly catalyzed the oxidation of HMF to FDCA, and the HMF conversion and FDCA yield both reached 100%. The oxidant NaClO could achieve a quantitative reaction (NaClO/HMF = 3:1(mol)). In addition, the reaction was conducted at mild room temperature and innovatively utilized a single aqueous system as the reaction solvent. This establishes a new method for the oxidative preparation of FDCA with the potential for technical scale-up.
研究领域:
化学工程
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）