Frontiers in Microbiology
·
2025
·
IF 4.5
Ting-Bin Hao,Peng-Yu Lai,Zhan Shu,Ran Liang,Zhi-Yun Chen,Ren-Long Huang,Yang Lu,Adili Alimujiang
Water scarcity is an escalating environmental concern. The model diatom, Phaeodactylum tricornutum, holds promise as a potential cell factory for the production of high-value natural compounds. However, its dependence on saline water cultivation restricts its use in areas facing water shortages. Although numerous studies have delved into the metabolic mechanisms of plants under water stress, there is a limited understanding when it comes to microalgae. In our study, we employed polyethylene glycol (PEG) to simulate water scarcity conditions, and assessed a range of parameters to elucidate the metabolic responses of P. tricornutum. Water stress induced the generation of reactive oxygen species (ROS), curtailed the photosynthetic growth rate, and amplified lipid content. Our insights shed light on the physiology of P. tricornutum when subjected to water stress, setting the stage for potential applications of microalgae biotechnology in regions grappling with water scarcity.
研究领域:
藻类生物学
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代谢组学
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产品关联
DOI
PubMed
高影响因子
FOOD MICROBIOLOGY
·
2023
·
IF 5.3
Shuxiang Liu,Ruimin Xue,Wen Qin,Xiaojuan Yang,Qinghua Ye,Qingping Wu
Lawn-harvest method uses a solid medium (e.g., tryptic soy agar, TSA) to produce bacterial lawns and is widely accepted for the culture of microorganisms in microbial studies of low-moisture foods (LMFs, foods with water activity less than 0.85). It produces desiccation-tolerant cells with higher D-values in LMFs; however, little is known about the molecular mechanisms underlying bacterial resistance . Salmonella enterica Enteritidis PT 30 ( S. Enteritidis), the most pertinent pathogen in LMFs, was cultured in TSA and tryptic soy broth (TSB). Cells were harvested and inoculated on filter papers to assess their performance under a relative humidity of 32 ± 2%. Transcriptome analysis of cultured cells during long-term desiccation (24, 72, and 168 h) was conducted in TruSeq PE Cluster Kit (Illumina) by paired-end methods. Lawn-cultured S. Enteritidis cells have stronger survivability (only decreased by 0.78 ± 0.12 log after 130 d of storage) and heat tolerance (higher D/β value) than those from the broth method. More desiccation genes of lawn-cultured cells were significantly upregulated from growth to long-term desiccation. Differentially expressed genes were the most enriched in the ribosome and sulfur metabolism pathways in the lawn- and broth-cultured groups. This study tracked the transcriptomic differences between two cultured groups in response to long-term desiccation stress and revealed some molecular mechanisms underlying their different suitability in microbial studies of LMFs.
研究领域:
微生物学
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食品安全
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分子生物学
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产品关联
DOI
PubMed
高影响因子
RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
·
2019
·Vol. 66
·期 5
·806-817
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IF 0.94
An Y. Q.,Sun L.,Wang X. J.,Sun R.,Cheng Z. Y.,Zhu Z. K.,Yan G. G.,Li Y. X.,Bai J. G.
Abstract This study investigated whether vanillic acid (VA) mitigated dehydration stress responses in blueberry ( Vaccinium corymbosum L.), and analyzed potential mechanisms mediating this activity. We pretreated 2‑year-old blueberry plants with 40 μM VA for two days, and then induced dehydration stress by irrigating with nutrient solution containing 10% (w/v) polyethylene glycol 6000 for two days. VA pretreatment increased the transcript levels of genes encoding eight antioxidant enzymes in leaves, including iron superoxide dismutase, chloroplast copper/zinc superoxide dismutase, cytoplasmic copper/zinc superoxide dismutase, catalase, guaiacol peroxidase, glutathione peroxidase, glutathione reductase, and dehydroascorbate reductase. These increased transcript levels were consistent with enhanced activities of superoxide dismutase and glutathione peroxidase and elevated contents of reduced glutathione and ascorbate. Subjecting the V--A‑treated blueberry to dehydration stress further enhanced expression levels of these genes, compared with the control plants subjected only to dehydration stress, increased the contents of endogenous VA, proline, and soluble sugars, enhanced the relative water content and osmotic potential, and reduced the levels of superoxide anion, hydrogen peroxide, and malondialdehyde. So pretreatment of blueberry with VA reduces lipid peroxidation and mitigates dehydration stress by enhancing the endogenous VA content, activating the expression of antioxidant enzyme genes, and increasing the levels of proline and soluble sugars.
研究领域:
植物生理学
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胁迫生物学
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分子生物学
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产品关联
DOI
高影响因子
Biology-Basel
·
2025
·Vol. 14
·期 9
·1303
·
IF 3.5
Tengyu Liu,Rui Han,Yuhan Jiang,Jiamin Sun,Haiyun Wu,Qigen Liu
Water depth is a crucial environmental factor influencing temperature, oxygen availability, light intensity, and ultimately the physiology, behavior, and distribution of aquatic animals. This study investigated the physiological responses of two common carp varieties—the Qingtian paddy field carp, a core component of the Qingtian rice-fish co-culture system, and the Xingguo red carp, a typical pond-domesticated species—to extreme shallow aquatic environments (5–20 cm depth). Since traditional blood sampling fails to capture dynamic stress levels, we used a real-time glucose biosensor combined with biochemical assays to continuously monitor stress and adaptive reactions. The results show that the Qingtian carp exhibits superior adaptation to shallow water conditions, marked by higher stress tolerance, faster recovery, and efficient lipid-based energy utilization. These findings highlight the value of biosensor-based approaches in aquaculture and affirm the critical role of the Qingtian carp in sustainable integrated rice–fish systems. The domestication of common carp in rice paddies (5–20 cm depth) is challenging, as the fish must withstand drastic fluctuations in temperature and dissolved oxygen, restricted movement, and bird predation without the option of diving. The effects of stress responses in different species of carp in shallow-water environments remain poorly understood, particularly with fluctuating water levels where real-time monitoring is challenging. This study employed a glucose biosensor system enabling real-time monitoring, together with biochemical analysis techniques capable of evaluating multiple physiological indicators, to investigate shallow-water adaptation in Qingtian paddy field carp and Xingguo red carp. Our results quantitatively reveal, for the first time, the differing physiological stress thresholds of the two carp strains under shallow water. The Qingtian paddy field carp exhibited a higher tolerance to shallow water and showed faster recovery from prolonged stress. Furthermore, the total cholesterol and triglyceride contents of Qingtian paddy field carp gradually increased with prolonged shallow-water stress, reflecting the activation of lipid metabolic pathways. These findings highlight the advantages of biosensor technology in aquatic stress research and a strong support of the core element of paddy domesticated carp in the Globally Important Agricultural Heritage Systems.
研究领域:
鱼类生理学
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水产养殖学
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环境应激生物学
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代谢生理学
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生物传感器技术
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可持续农业系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）