FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Yu Zhang,Zhiqing Lin,Qi Yao,Jian He,Haotian Feng,Wenyi Zhang,Zhigang Liu,Tian Yuan,Xuebo Liu,Long Ding
This study aimed to investigate the effect of milk peptides on irritable bowel syndrome (IBS). The mice were intragastrally administered with casein or whey protein hydrolysates at a dose of 1 g/kg body weight/day for 24 days and were subjected to Citrobacter rodentium infection and water avoidance stress from day 7 to 24. Results indicated that casein and whey protein hydrolysates effectively reduced diarrhea, anxiety, and visceral hypersensitivity in IBS mice.. Casein and whey protein hydrolysates regulated gut microbiota composition and increased the abundance of short-chain fatty acid-producing bacteria, such as Alloprevotella and Alistipes . Whey protein hydrolysate significantly increased the mRNA levels of zonula occludens-1 (ZO-1) and claudin-1 in the colon, while casein hydrolysate significantly improved the mRNA levels of occludin. Casein and whey protein hydrolysates both decreased the levels of pro-inflammatory cytokines including interleukin-6 (IL-6), interleukin-1β (IL-1β), and tumor necrosis factor-α (TNF-α), while increased the level of anti-inflammatory cytokine interleukin-10 (IL-10). Importantly, casein and whey protein hydrolysates significantly reduced colonic mast cell activation and decreased prostaglandin E2 (PGE2) production. Moreover, three novel casein-derived cyclooxygenase-2 (COX2)-inhibitory peptides RGPF, FPK, and NPW were identified with IC 50 values of 0.36 ± 0.03, 0.64 ± 0.01, and 1.10 ± 0.09 mM, respectively and predicted to form hydrogen bonds and hydrophobic interactions with the residues of the active site of COX2. This study highlighted the potential of milk peptides as bioactive ingredients in functional foods for managing IBS.
研究领域:
营养学
·
免疫学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2019
·
IF 3.571
Jia-Nan Yan,Jia-Run Han,Xin-Yu Jiang,Wen-Hui Shang,Wen-Gang Jin,Yi-Nan Du,Hai-Tao Wu
Involvement of DNA in gelation and microstructural properties of scallop (Patinopecten yessoensis) male gonad hydrolysates (SMGHs) and corresponding hybrid gel with κ-carrageenan (SMGHs/κ-C) was studied using DNase pretreatment. Although DNase pretreatment significantly transformed SMGHs from weak gels to liquid, it made SMGHs have a superior synergistic effect on gel formation with κ-C by evidence of 2.7-fold G′ and 1.1-fold melting temperature. However, the relaxation time (T21 and T23), functional groups, and flocculation behavior were comparable between SMGHs/κ-C and SMGHs/DNase/κ-C. Moreover, SMGHs/DNase/κ-C exhibited a denser network with more numerous patches and larger void spaces. These results suggest that DNA contributes to the gel formation of SMGHs whereas restricts more cationic peptides in SMGHs to bind sulfate groups in κ-C during gel formation.
研究领域:
食品科学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Food Chemistry-X
·
2025
·
IF 8.2
Xiao Wang,Hongru Liu,Hui He,Xinyu Luo,Hang Yang,Khushwant S. Bhullar,Bingjie Chen,Di Su,Tiantian Zhao,Wenzong Zhou
The objective of this study was to develop an efficient method for decolorizing and deodorizing Asian swamp eel (ASE) hydrolysate. At an activated carbon (AC) dosage of 0.5 % and pH 4.0, insoluble peptides from trichloroacetic acid (TCA), which have poor antioxidant activity, were effectively removed. Meanwhile, other nitrogenous fractions with better antioxidant activity were retained, achieving a decolorization rate of 74.02 ± 0.38 %. Orthogonal partial least squares discriminant analysis (OPLS-DA) successfully distinguished the solid phase microextraction gas chromatograph-mass spectrometery (SPME-GC–MS) results of samples treated with different deodorization methods (ASE stock hydrolysate, AC treatment, AC + complex bacteria, AC + yeast, and AC + cyclodextrin). A total of 22 differential volatile compounds with variable importance projection (VIP) values greater than 1 were identified. The combination of AC treatment and cyclodextrin encapsulation effectively removed undesirable odors, enhancing the sensory quality and market competitiveness of the final product.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food & Function
·
2019
·Vol. 10
·期 9
·5531-5543
·
IF 3.241
Zhiyu Fang,Yutao Chen,Ge Wang,Tao Feng,Meng Shen,Bin Xiao,Jingyi Gu,Weimin Wang,Jia Li,Yongjun Zhang
Caenorhabditis elegans is an important model organism for studying stress response mechanisms and identifying genetic pathways that influence longevity. The present study was designed to explore the in vivo-antioxidant potential and the probable mechanism of acid hydrolysates prepared from A. auricula polysaccharides (AAPHs-F) with the optimal acid hydrolysis conditions using Box–Behnken design, and C. elegans was used as a model organism. The effects of AAPHs-F on the locomotory behavior, lifespan, activities of antioxidant-related enzymes and levels of antioxidants in C. elegans were studied. In addition, the potential of AAPHs-F in up-regulating the expression of antioxidant-related genes in C. elegans, such as daf-16, skn-1, sod-1, sod-2 and sir-2.1, and the inhibition of cell apoptosis of C. elegans were also discussed. The results indicated that AAPHs-F could significantly increase the U-Turn frequency of nematodes, extend their lifespan, enhance antioxidant systems including superoxide dismutase (SOD) by 70.60%, catalase (CAT) by 73.45% and glutathione reductase (GR) by 258.68% (p < 0.01), increase the level of glutathione (GSH) by 110.22% (p < 0.01), and decrease the level of reactive oxygen species (ROS) and malondialdehyde (MDA) by 31.86% and 46.16% (p < 0.01), respectively. Quantitative real-time polymerase chain reaction (qRT-PCR) results showed that AAPHs-F could up-regulate mRNA expression levels of daf-16, skn-1, sir, sod-1 and sod-2 in wild-type C. elegans (>1.3 fold) when treated at a concentration of 0.1 mg mL−1 (p < 0.05 or p < 0.01). AAPHs-F was concluded to be heteropolysaccharides composed of mannose, glucose and galactose with a molar ratio of 12.7 : 3.25 : 1. The molecular weight of AAPHs-F was determined to be 885.37 Da. Furthermore, AAPHs-F is mainly formed of (1 → 3)-linked-α-D-glucopyranose, and carboxyl or acetamide is present in the molecule. In summary, our studies provide evidence that AAPHs-F helps improve the antioxidant defense system, and up-regulation of stress and longevity related genes suggests the possible involvement of these genes in the prevention of stress damage in C. elegans.
研究领域:
生物化学
·
分子生物学
·
遗传学
✓ 已验证
DOI
PubMed
高影响因子
Collagen and Leather
·
2024
·Vol. 6
·期 1
·1-11
·
IF 0
Dai Hongjie,Cao Yingnan,Fu Yu,Tang Mi,Feng Xin,Ma Liang,Zhang Yuhao
Fish scales, considered as low-value by-products, contain peptides and hydroxyapatite that can be applied to produce peptide chelated calcium directly. This study developed a sustainable and one-pot fabrication method for the peptide-chelated calcium from fish scale hydrolysates (FSP-Ca). During pepsin hydrolysis, the releases of peptides (FSP), calcium, and phosphate from fish scales occurred simultaneously, and the chelation was also effectively performed. After a 6-h hydrolysis, the yield of FSP was 46.18%, and the dissolution rate of calcium was 49.53%. Under the optimal conditions (pH 7, chelation time of 25 min, and chelation temperature of 48 °C), a high chelation rate of 86.16% was obtained, with a calcium content of 81.8 mg/g. The results of UV absorption, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and scanning electron microscopy (SEM) confirmed the successful chelation between FSP and calcium derived from fish scales. The –NH 2 , –COO – , N–H, C=O, C–H, and –OH groups in FSP participated in the formation of FSP-Ca. Graphical Abstract
研究领域:
食品科学
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生物材料
·
环境科学
✓ 已验证
DOI
PROCESS BIOCHEMISTRY
·
2015
·
IF 2.516
Xiang Zou,Jing Yang,Xu Tian,Meijing Guo,Zhenghua Li,Yunzheng Li
Polymalic acid (PMA) is a water-soluble biopolymer with many attractive properties for pharmaceutical applications. Its monomer, l -malic acid, is a potential C4 platform chemical, which is widely used in the food industry. In this study, a new strain with a high PMA yield from xylose was isolated and identified as Aureobasidium pullulans based on the morphological and phylogenetic analyzes. This strain produced PMA from xylose at a comparable or higher rate than glucose, and PMA produced from xylose had a similar molecular weight to that produced from glucose. A high final PMA titer of 80.4 g/L (91.2 g/L of malic acid after hydrolysis) was achieved using fed-batch fermentation with xylose in a 5-L fermentor at a PMA productivity of 0.52 g/L h. Corncob hydrolysate could be better utilized producing 28.6 g/L of PMA (or 32.4 g/L malic acid) in batch fermentation. PMA was further characterized for its chemical structure. This strain can be used to produce PMA and malic acid from xylose and lignocellulosic biomass hydrolysates.
研究领域:
生物化学
·
工业微生物学
✓ 已验证
产品关联
DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2020
·
IF 7.053
Wenqi Cao,Linfan Shi,Gengxin Hao,Jun Chen,Wuyin Weng
Microfluidized gelatin hydrolysates (MGH) with decreasing molecular weight from MGH-A to MGH-E were prepared, and their emulsion properties were investigated. The molecular weight, particle size, and emulsifying activity of MGH decreased as gelatin degraded by enzymatic hydrolysis with neutral protease increased. Dramatic decreases in the surface hydrophobicity and emulsifying stability of MGH were observed as the content of peptide fractions of 18 and 23 kDa increased. The average droplet sizes of emulsions based on MGH-A, MGH-B, MGH-C, and MGH-D were 622.1, 558.8, 389.7, and 1058.9 nm, respectively, and the MGH-E-based emulsion exhibited creaming. While the flow index of the MGH-A-based emulsion was greater than 1, those of the other emulsions were less than 1. Optical and confocal laser scanning microscopic images showed fine and uniform droplet distributions without flocculation or coalescence in the MGH-A-, MGH-B-, and MGH-C-based emulsions. The encapsulation efficiency (EE) of co-enzyme Q 10 (CoQ 10 ) in the spray-dried encapsulation prepared from MGH-B was 84.3%, and EE generally decreased with increasing or decreasing degradation degree of MGH. No obvious differences were observed among the surface images of encapsulations prepared using MGH-A, MGH-B, and MGH-C, and aggregated distributions were found in the MGH-D- and MGH-E-based encapsulations. The storage stability and in vitro bioaccessibility of CoQ 10 encapsulated by MGH-E were poorer than those of CoQ 10 encapsulated by the other wall materials. The results of the current study indicate that molecular weight and particle size have significant effects on the emulsion and encapsulation properties of MGH.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2016
·Vol. 182
·期 1
·55-66
·
IF 1.606
Fu Ruijie,Yao Kai,Zhang Qisheng,Jia Dongying,Zhao Jiayuan,Chi Yuanlong
A series of collagen hydrolysates (CHs) were prepared from pigskin shavings by using pepsin (PCH), trypsin (TCH), Alcalase (ACH), HCl (HCH), and NaOH (NCH). Their physicochemical properties, including degree of collagen hydrolysis, molecular weight distribution, electric charge, and microstructure, were investigated, and their flocculation performance was evaluated in a kaolin suspension, at varied pHs and concentrations. PCH exhibited high flocculation capability under acidic and neutral conditions, and its efficiency for removing suspended particles was approximately 80% at a concentration of 0.05 g/L. TCH, ACH, HCH, and NCH showed almost no flocculation capability. The flocculation capability of PCH could be mainly due to a combination of optimal molecular weight distribution and electric charge. This study could provide an environment-friendly natural flocculant and also proposes a promising approach for the reuse of collagen wastes.
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研究领域:
生物化学
·
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 3
·1079
·
IF 4.927
Henghui Zhang,Zhijun Zhang,Dongliang He,Shuying Li,Yongping Xu
Botanical oils are staple consumer goods globally, but as a by-product of oil crops, meal is of low utilization value and prone to causing environmental problems. The development of proteins in meal into bioactive peptides, such asPerillapeptide, through biotechnology can not only solve environmental problems, but also create more valuable nutritional additives. In the present work, the hydrolysis process ofPerillameal protein suitable for industrial application was optimized with the response surface methodology (RSM) on the basis of single-factor experiments. Alcalase was firstly selected as the best-performing among four proteases. Then, based on Alcalase, the optimal hydrolysis conditions were as follows: enzyme concentration of 7%, hydrolysis temperature of 61.4 °C, liquid-solid ratio of 22.33:1 (mL/g) and hydrolysis time of 4 h. Under these conditions, the degree of hydrolysis (DH) ofPerillameal protein was 26.23 ± 0.83% and the DPPH scavenging capacity of hydrolysate was 94.15 ± 1.12%. The soluble peptide or protein concentration ofPerillameal protein hydrolysate rose up to 5.24 ± 0.05 mg/mL, the ideal yield of which was estimated to be 17.9%. SDS-PAGE indicated that a large proportion of new bands in hydrolysate with small molecular weights appeared, which was different from the originalPerillameal protein. The present data contributed to further, more specific research on the separation, purification and identification of antioxidant peptide from the hydrolysate ofPerillameal protein. The results showed that the hydrolysis ofPerillameal protein could yield peptides with high antioxidant activity and potential applications as natural antioxidants in the food industry.Keywords:Perillameal protein;hydrolysate;response surface methodology;antioxidant activity;peptide
研究领域:
食品科学
·
生物技术
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2023
·Vol. 12
·期 18
·3468
·
IF 5.2
Chongzhen Sun,Hongyan Li,Xiaodan Hui,Yurong Ma,Zhina Yin,Qingsong Chen,Cong Chen,Hui Wu,Xiyang Wu
Mulberry leaf protein hydrolysates (HMP), and their in vitro gastrointestinal digests (GHMP), have shown favorable chemical antioxidant activities. The aim of this study is to investigate the potential protective effects of HMP and GHMP against 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced oxidative stress in human erythrocytes. The inhibition rate of hemolysis, the reactive oxygen species (ROS) level, the concentration of malondialdehyde (MDA), the reduced glutathione (GSH) and oxidized glutathione (GSSH), and the enzymatic activities of total superoxide dismutase (SOD), catalase (CAT), and cellular glutathione peroxidase (GSH-Px) were evaluated as the biomarkers of oxidative status in human erythrocytes. The results showed that HMP and GHMP effectively inhibit the occurrence of erythrocyte hemolysis in the range of 0.025–1.0 mg/mL, and the inhibition rates of HMP and GHMP reached 92% and 90% at concentrations of 0.4 mg/mL and 1.0 mg/mL, respectively. HMP and GHMP reduced the AAPH-induced oxidative hemolysis damage via suppressing the generation of ROS by inhibiting the formation of MDA, maintaining the balance of GSH/GSSG, and preserving the activities of the antioxidant enzymes, including SOD, GSH-Px, and CAT. Our findings revealed that both HMP and GHMP could be used as natural antioxidants, and have the potential for further application in the development of functional foods.Keywords:mulberry leaf;digested protein hydrolysates;erythrocytes;oxidative stress;natural antioxidants
研究领域:
营养学
·
食品科学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 3.8
Kanghong Wang,Chaozhong Xu,Wei Xiong,Shanshan Tong,Jia Ouyang,Xiaoli Gu
Efficient removal of fermentation inhibitors, particularly furan aldehydes like furfural is crucial for enhancing microbial fermentation efficiency in lignocellulosic biomass processing. In this study, zeolitic imidazolate framework-8 with different molar ratios were synthesized by regulating the Zn 2 + /2-methylimidazole molar ratio (from 1:4–1:15), and their structural properties and adsorption performance were systematically evaluated. The results showed that the pore structure was crucial to the adsorption performance, and the pore structure was optimal when the Zn 2+ /2-methylimidazole ratio was 1:8. The optimized zeolitic imidazole framework-8 showed rapid, selective and efficient adsorption of furfural under different operating conditions, thereby preventing the formation of inhibitors from affecting fermentation while minimizing sugar loss. This study provides a generalizable design principle for the development of adsorbents in biomass conversion. This work provides a scalable solution for biomass hydrolysate detoxification, with potential cost advantages due to the material’s reusability and high adsorption capacity compared to conventional resin-based methods.
研究领域:
生物工程
·
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Yuyan Wei,Ling Xie,Bertrand Muhoza,Qian Liu,Shiqing Song
The present study investigated the impact of four chicken liver protein hydrolysate-based cat food attractants on palatability. Aroma compounds were analyzed in these attractants, which were subsequently sprayed onto four different types of cat foods. Results revealed that CF4 exhibited the highest intake ratio and the first choice ratio, followed by CF2 sample. Orthogonal partial least-squares discriminant analysis (OPLS-DA) demonstrated significant differences among 50 volatile compounds identified from the four cat foods. Using variable importance in projection (VIP) values, we selected 17 key flavor compounds responsible for distinguishing between the four cat foods. Peptides with a molecular mass <180 Da showed correlation with nonanoic acid and cedrol, while those >3000 Da correlated with hexanoic acid ethyl ester. Regression coefficients (RCs) calculated from partial least-squares regression (PLSR) results showed positive correlations between compound content and palatability for six compounds, whereas negative correlations were observed for ten compounds. Validation experiments confirmed that nonanal, 2-propylpyridine, and 3-octen-2-one enhanced palatability and correlated with peptides ranging from 180 to 500 Da; conversely, nonanoic acid ethyl ester and 3-methyl-pentanoic acid reduced palatability and correlated with peptides ranging from 1000 to 3000 Da.
研究领域:
食品科学
·
动物营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）