Cuiyi Liang,Qiong Wang,Wen Wang,Carol Sze Ki Lin,Yunzi Hu,Wei Qi
Lignocellulosic biomass is the most abundant feedstock in production of bio-based fuels and materials. In order to achieve high conversion efficiency, enzyme cocktail has been assessed by numerous studies as a powerful tool to allocate diverse specific activities in one matrix. This study reported an efficient cellulase cocktail produced by co-cultivation of several Penicillium strains with lytic polysaccharide monooxygenases (LPMOs) producing strains. The optimal inoculum proportion was screened with hydrolysis yield as the indicator and through ANOVA analysis. When blending the consortium-derived enzyme cocktail with commercial cellulase preparation, the highest hydrolysis yield reached 88.7%, equivalent to 32% increase compared to using commercial cellulase alone. The behind structure-effect relationship between the substrate and enzymatic degradation was illustrated. Comparative analysis of the enzyme components, activities and the strains transcriptome provided a comprehensive evaluation and insights of the proposed Penicillium consortium, indicating it as a potent cellulase cocktail producer for enhancing lignocellulose biodegradation.
Tao Xu,Yongheng Zhong,Qi Chen,Lipeng Wu,Shengyang Ji,Bowen Yang,Yongzhu Zhang,Jianfu Shen,Baiyi Lu
This study applied ferulic acid, sinapic acid, and p -coumaric acid to synthesise esters with cassava starch and investigated their digestibility , GI and starch structure alterations. Starch after esterification contained less rapidly digestible starch (as low as 44.59%) and more resistant starch. Sinapic acid starch ester (SASE) had the lowest hydrolysis rate, which was 61.04% in this study. The GI of ferulic acid, p -coumaric acid, and sinapic acid starch esters were 53.5, 58.4, and 47.1, respectively. They were all significantly decreased compared with native cassava starch (81.5) (P < 0.05). The steric hindrance of phenolic acid moiety and increased hydrophobicity led to the reduced digestibility of starch esters. All these results indicated that phenolic acid starch esters could help to reduce postprandial blood glucose spikes, and therefore became a potential tool for diabetes management.
危险品化学品经营许可证（带存储）